Periplasmic expression of part of the major rotavirus capsid protein VP7 containing all the three antigenic regions in Escherichia coli.
Gene
; 177(1-2): 155-62, 1996 Oct 24.
Article
em En
| MEDLINE
| ID: mdl-8921861
ABSTRACT
Part of the porcine rotavirus outer capsid protein VP7 containing all the three antigenic regions was expressed as a chimeric protein with bacterial alkaline phosphatase (AP) in E. coli. The construct contains an ompF promoter, the DNA encoding the signal sequence and the first 12 amino acids of mature OmpF, part of vp7 and the DNA encoding mature AP. The chimeric protein is stable, retains the biological property of AP and ability to react with polyclonal antiserum against the virus, and can be exported through the bacterial inner membrane into the periplasm.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Capsídeo
/
Rotavirus
/
Proteínas do Capsídeo
/
Antígenos Virais
Idioma:
En
Revista:
Gene
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Austrália