Docosapentaenoic acid (22:5,n-3): metabolism and effect on prostacyclin production in endothelial cells.
Prostaglandins Leukot Essent Fatty Acids
; 55(4): 287-92, 1996 Oct.
Article
em En
| MEDLINE
| ID: mdl-8951998
ABSTRACT
Eicosapentaenoic acid (EPA, 205,n-3) and docosahexaenoic acid (DHA, 226, n-3), the two main fatty acids of fish oil, have been shown to inhibit prostacyclin production and to be actively interconverted, leading to the accumulation of docosapentaenoic acid (DPA, 225,n-3) in endothelial cell phospholipids. We have investigated the effect of supplementing endothelial cells with DPA on their capacity to produce prostacyclin. We found that endothelial cells incubated for 22 h with 25 microM DPA bound to albumin (fatty acid/albumin ratio of 1.3) produced two-fold less prostacyclin compared to control cells when stimulated with endogenous arachidonic acid-mobilizing agents such as bradykinin and calcium ionophore A23187. Since the formation of prostacyclin from 0.1-15 microM exogenous arachidonic acid was also reduced, it is suggested that prostacyclin inhibition observed in DPA-treated cells might not proceed from a reduction of arachidonic acid availability only. Such an inhibition was already observed after 1 h incubation of the cells with DPA, and with 2-20 times lower DPA concentrations. The inhibition might depend on EPA which was formed by retroconversion of DPA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endotélio Vascular
/
Epoprostenol
/
Ácidos Graxos Insaturados
Limite:
Animals
Idioma:
En
Revista:
Prostaglandins Leukot Essent Fatty Acids
Assunto da revista:
ENDOCRINOLOGIA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
França