The secondary structure of a fourteen-nucleotide fragment of the hairpin ribozyme.
Biochem Biophys Res Commun
; 232(2): 444-8, 1997 Mar 17.
Article
em En
| MEDLINE
| ID: mdl-9125198
ABSTRACT
A fourteen nucleotide RNA has been synthesized and its secondary structure investigated by non-denaturing polyacrylamide gel electrophoresis and 1H nuclear magnetic resonance spectroscopy. This fourteen nucleotide RNA corresponds to the hairpin loop end of the proposed secondary structure of the (-)sTRSV hairpin ribozyme. Non-denaturing polyacrylamide gel electrophoresis indicates that the fourteen nucleotide RNA exists predominantly as a monomer at a 1 mM strand concentration. Four peaks are found in the imino hydrogen region of the 1H NMR spectrum of the fourteen nucleotide RNA at this concentration. One-dimensional nuclear Overhauser effect spectroscopy of the imino hydrogen region of the 1H NMR spectrum gives results consistent with a model of the secondary structure of the fourteen nucleotide RNA having a three nucleotide hairpin loop and two double-stranded stems separated by a single bulged adenosine.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Catalítico
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos