Activation of human neutrophils by calcium carbonate polymorphs.
Dig Dis Sci
; 42(6): 1283-9, 1997 Jun.
Article
em En
| MEDLINE
| ID: mdl-9201096
ABSTRACT
Gallstone formation is frequently accompanied by inflammation of the gallbladder mucosa. Some gallstone components such as cholesterol, calcium bilirubinate, and calcium hydroxyapatite have been previously shown to activate neutrophils. We investigated the effect on neutrophils of the calcium carbonate polymorphs aragonite, calcite, and vaterite (all found in gallstones). By chemiluminescence, superoxide, and degranulation assay, all three crystals were shown to cause rapid activation of neutrophils. The potency of the crystals was aragonite > vaterite > calcite. In vivo, crystals may be plasma-protein-coated before they encounter neutrophils; therefore some experiments were repeated using crystals that had been preincubated with plasma. For aragonite and vaterite, protein adsorption decreased the chemiluminescence response by approximately 50%. In contrast, protein-coated calcite crystals elicited a greater chemiluminescence response than did uncoated crystals. In summary, the calcium carbonate polymorphs are potent activators of neutrophils and thus have the potential to contribute to gallstone-associated cholecystitis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbonato de Cálcio
/
Ativação de Neutrófilo
Tipo de estudo:
Etiology_studies
Limite:
Humans
Idioma:
En
Revista:
Dig Dis Sci
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos