Incorporation of cells into an ELISA system enhances antigen-driven lymphokine detection.
J Immunol Methods
; 205(2): 163-8, 1997 Jul 14.
Article
em En
| MEDLINE
| ID: mdl-9294598
ABSTRACT
The ability to measure successfully the levels of Th1 or Th2 cytokines during an in vitro antigen-driven, polyclonal T-cell response has proven to be more difficult than expected. Here we describe the development of a highly sensitive cell-based ELISA (celELISA) technique for the detection of murine Th1 and Th2 cytokines. The celELISA combines the quantification aspects of the conventional sandwich ELISA with the sensitivity of the ELISPOT assay. The celELISA was particularly useful for the improved detection of IL-2, IL-4, and to a lessor extent, IFN-gamma.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Ensaio de Imunoadsorção Enzimática
/
Linfocinas
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Reino Unido