Transfection of TTF-1 gene induces thyroglobulin gene expression in undifferentiated FRT cells.
Biochim Biophys Acta
; 1354(2): 171-81, 1997 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-9396634
ABSTRACT
The thyroglobulin gene, the substrate for thyroid hormone biosynthesis, is not expressed in the FRT cell line, which, even though it manifests the polarised epithelial phenotype, does not express any of the thyroid functional properties. Two transcription factors, TTF-1 and Pax-8, have been implicated in thyroid specific expression of the thyroglobulin gene. FRT cells contain Pax-8 but they lack TTF-1. In this paper, we show that transfection of TTF-1 expression vectors in FRT cells results in activation of thyroglobulin gene expression. If the expression vector encoded for TTF-1-ER, a fusion gene coding for the entire TTF-1 protein fused to the hormone-binding domain of the steroid receptor, under the control of the RSV promoter, thyroglobulin gene expression was controlled by estrogen. These data provide a direct demonstration that TTF-1 activates the chromosomal thyroglobulin promoter. Since transfection of TTF-1 expression vectors in non-thyroid cell types did not result in thyroglobulin gene expression, it is suggested that Pax-8, in addition, perhaps, to a specific cellular environment, might be required for thyroid specific expression of the thyroglobulin gene.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tireoglobulina
/
Glândula Tireoide
/
Fatores de Transcrição
/
Proteínas Nucleares
/
Regulação da Expressão Gênica
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Itália