Active uptake of serotonin as viability parameter of stored platelets.
Beitr Infusionsther Transfusionsmed
; 32: 84-7, 1994.
Article
em En
| MEDLINE
| ID: mdl-9422104
ABSTRACT
The platelet-plasma serotonin (5-HT) exchange in different apheresis products and platelet concentrates (PCs) from buffy coats was studied for quality control over a period of 7 days. In PCs with steadily decreasing pH due to inappropriate gas exchange the platelet 5-HT concentration increases significantly (> 10% positive netto uptake) during the 1st day of storage. Thereafter platelet serotonin drops rapidly with t1/2 = 1 day at pH < 6.5, but platelet counts were constant and 5-HT uptake was inhibited concomitantly. Plasma 5-HT increases as pH decreases. This can be enforced by competitive inhibition of serotonin uptake due to imipramine which also moderates the total 5-HT loss. However, in PC with stable pH the operative 5-HT pump keeps steady-state conditions. The efficacy of the transmembrane uptake mechanism can be adequately monitored by the assessment of the 5-HT platelet/plasma ratio as a new platelet viability parameter.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Preservação de Sangue
/
Serotonina
Limite:
Humans
Idioma:
En
Revista:
Beitr Infusionsther Transfusionsmed
Assunto da revista:
CIENCIAS DA NUTRICAO
/
HEMATOLOGIA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Alemanha