Delayed rejection of soluble tumor necrosis factor receptor-secreting tumor allografts.
Transplantation
; 65(1): 113-20, 1998 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-9448155
ABSTRACT
BACKGROUND:
Exogenous soluble tumor necrosis factor receptor (TNFR) has been shown to be an effective immunosuppressant. It has yet to be tested whether tissues secreting soluble TNFR, when transplanted into a foreign host, could locally generate immunosuppression and therefore manifest prolonged survival.METHODS:
A murine tumor line was transfected with the gene encoding a chimeric protein consisting of the extracellular domain of the human 75-kDa TNFR fused to the Fc region of the human IgG1 heavy chain. This tumor line was then injected into allogeneic recipients.RESULTS:
Transfected tumor cells were shown to secrete soluble TNFR. When transplanted into minor histocompatibility antigen-disparate allogeneic recipients, these tumor cells grew as a solid tumor and resisted rejection, whereas untransfected tumors and interleukin-4 receptor transfectant controls were rejected within 4 weeks. The resistance to rejection could be reversed by coadministration of an anti-TNFR monoclonal antibody.CONCLUSIONS:
Prolongation of graft survival can be achieved by genetically altering transplanted tissue to secrete soluble cytokine receptors.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores do Fator de Necrose Tumoral
/
Rejeição de Enxerto
/
Imunossupressores
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Transplantation
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos