Functional expression of the murine D2, D3, and D4 dopamine receptors in Xenopus laevis oocytes.
FEBS Lett
; 420(2-3): 191-5, 1997 Dec 29.
Article
em En
| MEDLINE
| ID: mdl-9459308
The different murine D2-type dopamine receptors (D2L, D2S, D3L, D3S, and D4) were expressed in Xenopus laevis oocytes. The D2-type receptors were all similarly and efficiently expressed in Xenopus oocytes and were shown to bind the D2 antagonist [125I]sulpride. They were all shown to activate Cl- influx upon agonist stimulation. Using the diagnostic inhibitor bumetanide, we were able to separate the Na+/K+/2Cl- cotransporter component of the Cl- influx from the total unidirectional Cl- influx. The D3L subtype was found to operate exclusively through the bumetanide-insensitive Cl- influx whereas the other D2-type receptors acted on the Na+/K+/2Cl- cotransporter as well. The pertussis toxin sensitivity of the receptor-activated chloride influx via the Na+/K+/2Cl- cotransporter varied between the various D2-type receptors showing that they may couple to different G proteins, and activate different second messenger systems.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Regulação da Expressão Gênica
/
Receptores Dopaminérgicos
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Israel