Development and applications of enhanced green fluorescent protein mutants.
Biotechniques
; 24(3): 462-6, 468-71, 1998 Mar.
Article
em En
| MEDLINE
| ID: mdl-9526659
ABSTRACT
The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Luminescentes
/
Mutação
Limite:
Animals
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos