Characterization of an ammonium transport protein from the peribacteroid membrane of soybean nodules.
Science
; 281(5380): 1202-6, 1998 Aug 21.
Article
em En
| MEDLINE
| ID: mdl-9712587
ABSTRACT
Nitrogen-fixing bacteroids in legume root nodules are surrounded by the plant-derived peribacteroid membrane, which controls nutrient transfer between the symbionts. A nodule complementary DNA (GmSAT1) encoding an ammonium transporter has been isolated from soybean. GmSAT1 is preferentially transcribed in nodules and immunoblotting indicates that GmSAT1 is located on the peribacteroid membrane. [14C]methylammonium uptake and patch-clamp analysis of yeast expressing GmSAT1 demonstrated that it shares properties with a soybean peribacteroid membrane NH4+ channel described elsewhere. GmSAT1 is likely to be involved in the transfer of fixed nitrogen from the bacteroid to the host.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glycine max
/
Proteínas de Transporte
/
Proteínas de Soja
/
Proteínas de Transporte de Cátions
/
Compostos de Amônio Quaternário
Idioma:
En
Revista:
Science
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Austrália