Cloning and expression of the Campylobacter jejuni lon gene detected by RNA arbitrarily primed PCR.
FEMS Microbiol Lett
; 165(2): 329-34, 1998 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-9742705
ABSTRACT
Fingerprinting of RNA by arbitrarily primed PCR was used to identify a heat-inducible gene in Campylobacter jejuni. Comparing RNA fingerprints from C. jejuni cells before and after 20 min of heat shock at 48 degrees C, a differentially amplified PCR product was identified which displayed a high degree of homology to bacterial lon genes. By screening C. jejuni genomic libraries, the entire lon gene was cloned and sequenced. It encodes a protein of 791 amino acids with a calculated molecular mass of 90.2 kDa. Alignment of the Lon amino acid sequence with that of other bacterial species revealed an overall identity of up to 56.6% (Helicobacter pylori). Northern and RNA dot blot experiments confirmed heat induction of the C. jejuni lon gene, revealing a maximum 6-8-fold increase in the level of specific mRNA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Serina Endopeptidases
/
Reação em Cadeia da Polimerase
/
Campylobacter jejuni
/
Genes Bacterianos
/
Proteínas de Choque Térmico
Limite:
Humans
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Alemanha