Influence of N-acetylcysteine treatment on endotoxin-induced microcirculatory disturbances.
Intensive Care Med
; 24(9): 967-72, 1998 Sep.
Article
em En
| MEDLINE
| ID: mdl-9803334
ABSTRACT
OBJECTIVES:
To determine the influence of N-acetylcysteine (NAC) in a treatment model, its effects on endotoxin-induced leukocyte-endothelial cell adhesion, vascular leakage, and venular microhemodynamics in postcapillary venules of rat mesentery.DESIGN:
Prospective, randomized, controlled, experimental study.SETTING:
Animal research laboratory.SUBJECTS:
40 male Wistar rats.INTERVENTIONS:
The rats randomly received one of four treatments infusion of saline (SAL) or Escherichia coli lipopolysaccharides (LPS) followed by treatment with saline (SAL) or NAC (150 mg.kg-1 body weight) 30 min after induction of endotoxemia. MEASUREMENTS AND MAINRESULTS:
Leukocyte adherence, red blood cell velocity, and vessel diameters in postcapillary venules of rat mesentery were evaluated every 30 min over a period of 120 min using in vivo videomicroscopy. Vascular permeability was determined by measuring the extravasation of fluorescence-labeled albumin. Venular wall shear rate was calculated from red cell velocity, and vessel diameter. NAC in rats without endotoxemia (SAL + NAC group) compared to the control group (SAL + SAL) did not change microcirculatory parameters in postcapillary venules of rat mesentery. In both LPS-treated groups (LPS + SAL and LPS + NAC), leukocyte adherence increased after just 30 min. NAC treatment prevented a further increase in leukocyte adherence and attenuated the extravasation of fluorescence-labeled albumin during endotoxemia. Venular diameters remained unchanged, while erythrocyte velocity decreased in the LPS + SAL group. This led to a lower venular wall shear rate in this group.CONCLUSIONS:
Treatment with NAC attenuates endotoxin-induced leukocyte adherence and macromolecular leakage in postcapillary venules of rat mesentery, showing that NAC is also effective after the onset of endotoxemia.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetilcisteína
/
Vênulas
/
Permeabilidade Capilar
/
Endotoxemia
/
Eritrócitos
/
Leucócitos
Tipo de estudo:
Observational_studies
Limite:
Animals
Idioma:
En
Revista:
Intensive Care Med
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Alemanha