Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization.
J Cell Biol
; 77(3): 743-51, 1978 Jun.
Article
em En
| MEDLINE
| ID: mdl-98531
ABSTRACT
The preparation of a specific antiserum (anti-CaBP) against the calcium-binding protein (CaBP) of the chorioallantoic membrane (CAM) is described. The anti-CaBP appeared to be specific for the CaBP by immunodiffusion and immunoelectrophoresis. Application of the anti-CaBP in immunofluorescence histochemistry revealed that the CaBP is present in the CAM only at developmental ages corresponding with the expression of the calcium transport function of the membrane. Furthermore, the CaBP is localized to the ectoderm of the CAM, appears to be exposed to the entire external surface of the ectoderm, and can be shown to be associated with cells enzymatically dissociated from the CAM. These results are consistent with a functional role of the CaBP in the CAM calcium transport process.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Membranas Extraembrionárias
Limite:
Animals
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
1978
Tipo de documento:
Article