Scanning electron microscopy of rat's liver: application of freeze-fracture and freeze-drying techniques
Brooks, Stanley E. H; Haggis, Geoffrey H.
Lab Invest
; 29(1): 60-4, 1973.
Article
in English
| MedCarib | ID: med-12184
Portal vein perfusion of rat's liver was carried out with Ringer's solution followed by 2.5 per cent glutaraldehyde. Liver samples were postifixed in osmium tetroxide, washed in distilled water, snap-frozen in Freon-12, and fractured at -150§C. After freeze-drying at -80§C., a thin layer of carbon and gold was applied under vacuum. Under the scanning electron microscope, the sinusoidal fenestrations and the relationships of the endothelial cells to hepatocyte microvilli and reticulin fibres were delineated. Images of bile canaliculi and adjoining plasma membranes were obtained. These techniques have great potential for the study of a variety of liver diseases. (AU)
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