The
electron microscope provides easy confirmation in
Gaucher's disease. This may be done with conventional ultrathin sections or, more quickly, by
negative staining of
tissues minced in 1 percent
ammonium acetate. Typical helical structures associated with
glucocerebroside storage were easily demonstrated with
tissues that had been fixed in
formaldehyde solution and embedded in
paraffin blocks.
Similar success was obtained with splenic
tissue that had been in a
museum jar for 17 years.(AU)