RESUMEN
Staphylococcus aureus is the most common contagious pathogen associated with bovine subclinical mastitis. Current diagnosis of S. aureus mastitis is based on bacteriological culture of milk samples and somatic cell counts, which lack either sensitivity or specificity. Identification of milk proteins that contribute to host defense and their variable responses to pathogenic stimuli would enable the characterization of putative biomarkers of subclinical mastitis. To accomplish this, milk whey samples from healthy and mastitic dairy cows were analyzed using a label-free quantitative proteomics approach. In total, 90 proteins were identified, of which 25 showed significant differential abundance between healthy and mastitic samples. In silico functional analyses indicated the involvement of the differentially abundant proteins in biological mechanisms and signaling pathways related to host defense including pathogen-recognition, direct antimicrobial function, and the acute-phase response. This proteomics and bioinformatics analysis not only facilitates the identification of putative biomarkers of S. aureus subclinical mastitis but also recapitulates previous findings demonstrating the abundance of host defense proteins in intramammary infection. All mass spectrometry data are available via ProteomeXchange with identifier PXD007516.
Asunto(s)
Proteínas de Fase Aguda/genética , Péptidos Catiónicos Antimicrobianos/genética , Mastitis Bovina/genética , Proteoma/genética , Infecciones Estafilocócicas/genética , Proteína de Suero de Leche/genética , Proteínas de Fase Aguda/inmunología , Proteínas de Fase Aguda/aislamiento & purificación , Animales , Péptidos Catiónicos Antimicrobianos/inmunología , Péptidos Catiónicos Antimicrobianos/aislamiento & purificación , Bovinos , Femenino , Mastitis Bovina/inmunología , Mapeo de Interacción de Proteínas , Proteoma/inmunología , Proteoma/aislamiento & purificación , Proteómica/métodos , Infecciones Estafilocócicas/inmunología , Staphylococcus aureus/crecimiento & desarrollo , Staphylococcus aureus/patogenicidad , Suero Lácteo/química , Proteína de Suero de Leche/inmunología , Proteína de Suero de Leche/aislamiento & purificaciónRESUMEN
This dataset is associated with our research article 'Identification of host-defence related proteins using label-free quantitative proteomic analysis of milk whey from cows with Staphylococcus aureus Subclinical mastitis' published in International Journal of Molecular Sciences. Milk samples were collected from cows suffering from S. aureus-associated subclinical mastitis and the proteins abundance were identified in comparison with samples collected from the control cows using liquid chromatography-mass spectrometry (LC-MS)-based label free proteomics analysis. Following the MS measurements, the raw spectra were processed using MaxQuant-Andromeda software and the protein identification was carried out through a search against Uniprot FASTA files of the Bos taurus reference proteome. Perseus software analysis was applied for computation of protein abundance. The raw file presented in this DiB was deposition at PRIDE repository in the PRIDE repository with the dataset identifier PXD007516.