Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging.

In this study, a laparoscopic imaging device and a light source able to select wavelengths by bandpass filters were developed to perform multispectral imaging (MSI) using over 1000 nm near-infrared (OTN-NIR) on regions under a laparoscope. Subsequently, MSI (wavelengths: 1000-1400 nm) was performed using the built device on nine live mice before and after tumor implantation. The normal and tumor pixels captured within the mice were used as teaching data sets, and the tumor-implanted mice data were classified using a neural network applied following a leave-one-out cross-validation procedure. The system provided a specificity of 89.5%, a sensitivity of 53.5%, and an accuracy of 87.8% for subcutaneous tumor discrimination. Aggregated true-positive (TP) pixels were confirmed in all tumor-implanted mice, which indicated that the laparoscopic OTN-NIR MSI could potentially be applied in vivo for classifying target lesions such as cancer in deep tissues.

Evaluation of laser-induced plasma ablation focusing on the difference in pulse duration.

A pulsed laser cause vaporization of tissue by plasma if a laser can provide high-density energy within a very short pulse duration. Such phenomena are called laser-induced plasma ablation. The influence of the laser-induced plasma ablation for tissue is unclear because the ablation mechanism is differing regardless of two lasers provide almost the same power density. The two kinds of lasers' vaporization mechanism (Nanosecond laser output could cause an optical breakdown in the air depending on power density and pulse duration of the laser and Femtosecond laser output could cause a breakdown only on solids surface since pulse irradiation time is shorter than energy transfer time) are evaluated by using thermal damage and destruction of tissue. The experimental results show that nanosecond laser caused vaporization without thermal damage and destruction at the tissue approximant 300 µm away from the ablation area. The pulsed laser which has high power density and longer pulse duration than energy transfer time is suitable for plasma ablation not depending on thermal process.