Regional Changes in Posterior Corneal Surface During a 6-Month Follow-up Period After tPRK, FS-LASIK, and SMILE.

PURPOSE: To investigate changes in corneal curvature in different zones of the posterior corneal surface during a 6-month follow-up period after transepithelial photorefractive keratectomy (tPRK), femtosecond laser-assisted excimer laser in situ keratomileusis (FS-LASIK), and small incision lenticule extraction (SMILE). METHODS: The study included a total of 202 eyes, including 65, 77, and 60 that underwent tPRK, FS-LASIK, and SMILE, respectively. Elevation data for the posterior surface were obtained preoperatively (pre), as well as 1 week (pos1w), 1 month (pos1m), 3 months (pos3m), and 6 months (pos6m) postoperatively. Changes in posterior corneal curvature (M) were analyzed in the central (diameter: 0 to 3 mm), paracentral (diameter: 3 to 6 mm), and peripheral (diameter: 6 to 9 mm) regions. RESULTS: Over all follow-up periods, the central region of the posterior surface in all patients became flatter (P < .05), with FS-LASIK showing the largest change, whereas the paracentral and peripheral regions became steeper. The posterior curvature changes between pre and pos6m, determined before and after correction for ablated stromal depth, tended to follow similar trends in the three regions and after the three surgeries. There was also no significant correlation (P > .05) between the changes in the mean curvature (M, recorded between pre and pos6m) and each of the refractive error corrections, the changes in spherical aberration postoperatively, the optical zone diameter, ablated stromal depth, and residual stromal bed thickness in the central and peripheral regions, but the correlation was significant in the paracentral region. CONCLUSIONS: The postoperative changes in posterior corneal shape followed different trends in the central, paracentral, and peripheral regions. The FS-LASIK group exhibited the most notable changes in posterior corneal curvature, especially in the central region. These changes were statistically correlated with variations in spherical aberration, and ablated and residential stromal thickness in the paracentral region. [J Refract Surg. 2022;38(11):708-715.].

Long-term Effects of Riboflavin Ultraviolet-A-Induced CXL With Different Irradiances on the Biomechanics of In Vivo Rabbit Corneas.

PURPOSE: To evaluate the long-term effects of ultraviolet-A corneal cross-linking (CXL) with different irrandiances on the biomechanical properties of rabbit corneas and the corresponding changes in stromal microstructure. METHODS: The study involved the left eyes of 85 healthy white Japanese rabbits, randomly divided into five groups (n = 16 to 18 each). After removing the epithelium, the first four groups were exposed to riboflavin (0.22% concentration by volume) and ultraviolet-A (370 nm) at different CXL irradiations but with the same total dose (5.4 J/cm2). The four groups were defined as standard CXL (SCXL; 3 mW/cm2 for 30 minutes, n = 17), accelerated CXL1 (ACXL1; 9 mW/cm2 for 10 minutes, n = 16), accelerated CXL2 (ACXL2; 18 mW/cm2 for 5 minutes, n = 17), and accelerated CXL3 (ACXL3; 30 mW/cm2 for 3 minutes, n = 17). The control group (n = 18) was treated with riboflavin without ultraviolet-A exposure. Nine months after CXL, 10 corneas from each group were tested ex vivo under inflation, and the tangent modulus (Et) was estimated using an inverse analysis process. The remaining six to eight specimens in each group were examined by electron microscopy to determine the mean fibril diameter and interfibrillar spacing. RESULTS: The SCXL and ACXL1 groups showed statistically significant differences in Et at all stresses (0.005, 0.010, and 0.015 MPa) analyzed compared to the control group (all P < .01), but the differences were non-significant in the ACXL3 group (P = 1.000, .785, and .679, respectively). For the ACXL2 group, there was no statistical difference in Et under the low stress of 0.005 MPa (P = .155), but the differences became significant at 0.010 and 0.015 MPa when compared with the control group (all P < .05). CONCLUSIONS: CXL had a significant effect on corneal biomechanics in both standard and accelerated procedures. However, standard CXL was the most effective, and this effectiveness decreased gradually with increasing ultraviolet-A power intensity. [J Refract Surg. 2022;38(6):389-397.].

Changes in Corneal Biomechanical Properties in PRK Followed by Two Accelerated CXL Energy Doses in Rabbit Eyes.

PURPOSE: To evaluate whether photorefractive keratectomy (PRK) combined with the two commonly delivered energy doses in accelerated corneal cross-linking (A-CXL) could help the cornea maintain its preoperative stiffness level. METHODS: A total of 72 corneas of 36 healthy white Japanese rabbits were randomly divided into four equal groups. The groups included an untreated control group and three that had undergone PRK. After tissue ablation, one of the latter three groups (PRK group) was left untreated, whereas the other two were exposed to riboflavin (0.22% concentration by volume) and ultraviolet-A (370 nm) with the same irradiation (30 mW/cm2) but different CXL energy doses of 1.8 J/cm2 (PXL group) and 2.7 J/cm2 (PXH group). Dynamic Scheimpflug analyzer (Corvis ST; Oculus Optikgeräte GmbH) measurements of stiffness parameter at first applanation (SP-A1), Stress-Strain Index (SSI), and other dynamic corneal response parameters were taken 3 days preoperatively and 1 month postoperatively. Subsequently, ex vivo inflation testing was performed and the tangent modulus of each specimen was estimated using an inverse analysis process. RESULTS: In comparison to the control group, the tangent modulus at a stress of 10 kPa decreased by 8.9% in the PRK group and increased by 10.6% and 22.4% in the PXL and PXH groups, respectively. SP-A1 decreased postoperatively in the PRK group (P < .05), indicating an overall stiffness reduction of -7.4, -3.5, and -5.3 mm Hg/mm in PRK, PXL, and PXH groups, respectively. The material stiffness parameter SSI remained almost unchanged in the PRK group (P = .989), increased slightly in the PXL group (8.3%, P = .077), and increased significantly in the PXH group (11.1%) (P < .05). CONCLUSIONS: Biomechanical deterioration following PRK was significant and could not be fully compensated for by ACXL with either 1.8 or 2.7 J/cm2 doses. The increased value of corneal overall stiffness was higher in A-CXL with 2.7 J/cm2 energy than with 1.8 J/cm2 energy. [J Refract Surg. 2021;37(12):853-860.].

Effect of Mydriasis-Caused Intraocular Pressure Changes on Corneal Biomechanical Metrics.

Purpose: To evaluate the dependence of biomechanical metrics on intraocular pressure (IOP). Methods: 233 refractive surgery patients were included in this study-all were examined 3 times with the Corvis ST before and after dilation, and the differences (∆) in the main device parameters were assessed. The data collected included the biomechanically corrected IOP (bIOP), the central corneal thickness (CCT), and six dynamic corneal response (DCR) parameters, namely DA, DARatio2mm, IIR, SP-A1, CBI, and SSI. Participants were divided into three groups according to the changes in patients' bIOP after mydriasis. Results: Intra-operator repeatability was generally high in most of the DCR parameters obtained before and after dilation. The mean changes in bIOP and CCT after dilation were -0.12 ± 1.36 mmHg and 1.95 ± 5.23 µm, respectively. Only ∆DARatio2mm, ∆IIR, and ∆CBI exhibited a statistically significant correlation with ∆CCT (p < 0.05). The changes in all DCR parameters, especially ∆DA and ∆SP-A1 were also correlated with ∆bIOP (p < 0.01)-a 1-mmHg change in bIOP was associated, on average, with 5.612 and -0.037 units of change in SP-A1 and DA, respectively. In contrast, the weakest correlation with ∆bIOP was exhibited by ∆SSI. Conclusion: Most corneal DCR parameters, provided by the Corvis ST, were correlated with IOP, and more weakly with CCT. Changes experienced in CCT and IOP should therefore be considered in studies on corneal biomechanics and how it is affected by disease progression and surgical or medical procedures.