RESUMEN
The retention of fluoride in clean and plaque-covered demineralized enamel in vivo was measured 1 wk after a single application of a MFP solution with low or high content of free fluoride. Demineralization of the enamel was induced in vivo during a 4-wk period prior to application of fluoride by applying orthodontic bands on a pair of premolars scheduled for extraction of orthodontic reasons. The band from one tooth of each pair was then removed and the tooth cleaned (clean enamel lesion). The band on the contralateral tooth (plaque-covered enamel lesion) was left in place. The MFP solutions were applied and the teeth extracted after one more week. The chemical form of fluorine in the lesions was analyzed by conventional acid etching of the enamel combined with KOH dissolution and by a nondestructive surface analysis using ESCA to detect intact monofluorophosphate ions. The ESCA measurements showed that MFP was only present in the clean enamel lesions, indicating complete hydrolysis by dental plaque. Alkali soluble fluoride could be extracted from both plaque-covered and clean enamel lesions. Conflicting results regarding the amount of fluoride uptake in the lesions were obtained with the acid etching and the ESCA technique. It was thus not clearly established whether plaque enhanced fluoride uptake in carious lesions after MFP application.