Moving from ecological impacts to social vulnerability in data-scarce places.

Managers need to trace social impacts and vulnerability caused by environmental change all the way to its driving forces to target key system components for intervention. However, most available scientific evidence deals with either the ecological impacts of direct drivers or the value of ecosystem benefits to people. Our matrix-based tool combines these types of evidence to make environmental management problems traceable through a structured yet flexible procedure. The tool consists of a series of matrices that sequentially link direct drivers of environmental change, landscapes, ecological conditions, benefits to people, and stakeholder types. Qualitative matrices result from the integration and synthesis of available evidence from literature reviews, and where data is scarce, these are used to elicit quantitative scores from expert opinion. Expert scoring of links and multiplication of matrices allow for estimating the impacts of each driver of environmental change on each stakeholder type and using this information as input to assess stakeholders' vulnerability through impact-influence diagrams. Applying the tool to the Argentine Gran Chaco, a globally threatened ecoregion, yielded a transparent and reliable picture of this data-scarce place, with important management implications. Tracing stakeholder impacts back to direct drivers confirmed that further encroachment of cleared areas around indigenous lands will increase the vulnerability of this social group. Also, assessing confidence levels for every social-ecological link suggested that incentivizing peasant farmers to restore natural forage supply represents a management opportunity to reverse degradation. Our tool makes interdisciplinary frameworks of linked ecological and social systems operational so managers can use the best available knowledge of a place and account for uncertainty to make environmental management decisions.

Cystic echinococcosis in South America: systematic review of species and genotypes of Echinococcus granulosus sensu lato in humans and natural domestic hosts.

OBJECTIVE: To systematically review publications on Echinococcus granulosus sensu lato species/genotypes reported in domestic intermediate and definitive hosts in South America and in human cases worldwide, taking into account those articles where DNA sequencing was performed; and to analyse the density of each type of livestock that can act as intermediate host, and features of medical importance such as cyst organ location. METHODS: Literature search in numerous databases. We included only articles where samples were genotyped by sequencing since to date it is the most accurate method to unambiguously identify all E. granulosus s. l. genotypes. Also, we report new E. granulosus s. l. samples from Argentina and Uruguay analysed by sequencing of cox1 gene. RESULTS: In South America, five countries have cystic echinococcosis cases for which sequencing data are available: Argentina, Brazil, Chile, Peru and Uruguay, adding up 1534 cases. E. granulosus s. s. (G1) accounts for most of the global burden of human and livestock cases. Also, E. canadensis (G6) plays a significant role in human cystic echinococcosis. Likewise, worldwide analysis of human cases showed that 72.9% are caused by E. granulosus s. s. (G1) and 12.2% and 9.6% by E. canadensis G6 and G7, respectively. CONCLUSIONS: E. granulosus s. s. (G1) accounts for most of the global burden followed by E. canadensis (G6 and G7) in South America and worldwide. This information should be taken into account to suit local cystic echinococcosis control and prevention programmes according to each molecular epidemiological situation.

Nature representation in South American protected areas: country contrasts and conservation priorities.

BACKGROUND: South America faces strong environmental pressures as a result of agriculture and infrastructure expansion and also of demographic growth, demanding immediate action to preserve natural assets by establishing protected areas. Currently, 7.1% of the (sub)continent is under strict conservation categories (I to IV, IUCN), but the spatial distribution of these 1.3 × 106 km2 is poorly understood. We evaluated the representation of nature within the networks of protected areas, map conservation priorities and assess demographic, economic or geopolitical causes of existing protection patterns. METHODS: We characterized nature representation by looking at two components: the extent and the equality of protection. The first refers to the fraction of territory under protection, while the second refers to the homogeneity in the distribution along natural conditions of this protected fraction. We characterized natural conditions by either 113 biogeographical units (specifically, ecoregions) or a series of limited and significant climatic, topographic and edaphic traits. We analyzed representation every ten years since 1960 at national and continental levels. In the physical approach, histograms allowed us to map the degree of conservation priorities. Finally, we ranked the importance of different economic or geopolitical variables driving the observed distributions with a random forest technique. RESULTS: Nature representation varied across countries in spite of its priority in conservation agendas. In Brazil, Peru and Argentina there are still natural conditions with no formal protection, while in Bolivia and Venezuela, protected areas incorporate the natural diversity in a more balanced manner. As protected networks have increased their extent, so did their equality across and within countries over time. Our maps revealed as top continental priorities the southern temperate, subhumid and fertile lowland environments, and other country-specific areas. Protection extent was generally driven by a low population density and isolation, while other variables like distance to frontiers, were relevant only locally (e.g., in Argentina). DISCUSSION: Our description of the spatial distribution of protected areas can help societies and governments to improve the allocation of conservation efforts. We identified the main limitations that future conservation efforts will face, as protection was generally driven by the opportunities provided by low population density and isolation. From a methodological perspective, the physical approach reveals new properties of protection and provides tools to explore nature representation at different spatial, temporal and conceptual levels, complementing the traditional ones based on biodiversity or biogeographical attributes.

Long-term Satellite NDVI Data Sets: Evaluating Their Ability to Detect Ecosystem Functional Changes in South America.

In the last decades, South American ecosystems underwent important functional modifications due to climate alterations and direct human intervention on land use and land cover. Among remotely sensed data sets, NOAA-AVHRR "Normalized Difference Vegetation Index" (NDVI) represents one of the most powerful tools to evaluate these changes thanks to their extended temporal coverage. In this paper we explored the possibilities and limitations of three commonly used NOAA-AVHRR NDVI series (PAL, GIMMS and FASIR) to detect ecosystem functional changes in the South American continent. We performed pixel-based linear regressions for four NDVI variables (average annual, maximum annual, minimum annual and intra-annual coefficient of variation) for the 1982-1999 period and (1) analyzed the convergences and divergences of significant multi-annual trends identified across all series, (2) explored the degree of aggregation of the trends using the O-ring statistic, and (3) evaluated observed trends using independent information on ecosystem functional changes in five focal regions. Several differences arose in terms of the patterns of change (the sign, localization and total number of pixels with changes). FASIR presented the highest proportion of changing pixels (32.7%) and GIMMS the lowest (16.2%). PAL and FASIR data sets showed the highest agreement, with a convergence of detected trends on 71.2% of the pixels. Even though positive and negative changes showed substantial spatial aggregation, important differences in the scale of aggregation emerged among the series, with GIMMS showing the smaller scale (≤11 pixels). The independent evaluations suggest higher accuracy in the detection of ecosystem changes among PAL and FASIR series than with GIMMS, as they detected trends that match expected shifts. In fact, this last series eliminated most of the long term patterns over the continent. For example, in the "Eastern Paraguay" and "Uruguay River margins" focal regions, the extensive changes due to land use and land cover change expansion were detected by PAL and FASIR, but completely ignored by GIMMS. Although the technical explanation of the differences remains unclear and needs further exploration, we found that the evaluation of this type of remote sensing tools should not only be focused at the level of assumptions (i.e. physical or mathematical aspects of image processing), but also at the level of results (i.e. contrasting observed patterns with independent proofs of change). We finally present the online collaborative initiative "Land ecosystem change utility for South America", which facilitates this type of evaluations and helps to identify the most important functional changes of the continent.

Opportunities drive the global distribution of protected areas.

BACKGROUND: Protected areas, regarded today as a cornerstone of nature conservation, result from an array of multiple motivations and opportunities. We explored at global and regional levels the current distribution of protected areas along biophysical, human, and biological gradients, and assessed to what extent protection has pursued (i) a balanced representation of biophysical environments, (ii) a set of preferred conditions (biological, spiritual, economic, or geopolitical), or (iii) existing opportunities for conservation regardless of any representation or preference criteria. METHODS: We used histograms to describe the distribution of terrestrial protected areas along biophysical, human, and biological independent gradients and linear and non-linear regression and correlation analyses to describe the sign, shape, and strength of the relationships. We used a random forest analysis to rank the importance of different variables related to conservation preferences and opportunity drivers, and an evenness metric to quantify representativeness. RESULTS: We find that protection at a global level is primarily driven by the opportunities provided by isolation and a low population density (variable importance = 34.6 and 19.9, respectively). Preferences play a secondary role, with a bias towards tourism attractiveness and proximity to international borders (variable importance = 12.7 and 3.4, respectively). Opportunities shape protection strongly in "North America & Australia-NZ" and "Latin America & Caribbean," while the importance of the representativeness of biophysical environments is higher in "Sub-Saharan Africa" (1.3 times the average of other regions). DISCUSSION: Environmental representativeness and biodiversity protection are top priorities in land conservation agendas. However, our results suggest that they have been minor players driving current protection at both global and regional levels. Attempts to increase their relevance will necessarily have to recognize the predominant opportunistic nature that the establishment of protected areas has had until present times.

Implementation of new tools in molecular epidemiology studies of Echinococcus granulosus sensu lato in South America.

The aim of this work was to determine Echinococcus granulosus sensu lato species and genotypes in intermediate and definitive hosts and in human isolates from endemic regions of Argentina and Brazil including those where no molecular data is available by a combination of classical and alternative molecular tools. A total of 227 samples were isolated from humans, natural intermediate and definitive hosts. Amplification of cytochrome c oxidase subunit I gene fragment was performed and a combination of AluI digestion assay, High Resolution Melting analysis (HRM) assay and DNA sequencing was implemented for Echinococcus species/genotype determination. E. granulosus sensu stricto (G1) was found in sheep (n=35), cattle (n=67) and dogs (n=5); E. ortleppi (G5) in humans (n=3) and cattle (n=108); E. canadensis (G6) in humans (n=2) and E. canadensis (G7) in pigs (n=7). We reported for the first time the presence of E. ortleppi (G5) and E. canadensis (G6) in humans from San Juan and Catamarca Argentinean provinces and E. canadensis (G7) in pigs from Cordoba Argentinean province. In this work, we widened molecular epidemiology studies of E. granulosus s. l. in South America by analyzing several isolates from definitive and intermediate hosts, including humans from endemic regions were such information was scarce or unavailable. The presence of different species/genotypes in the same region and host species reinforce the need of rapid and specific techniques for accurate determination of Echinococcus species such as the ones proposed in this work.

Vegetation Productivity in Natural vs. Cultivated Systems along Water Availability Gradients in the Dry Subtropics.

The dry subtropics are subject to a rapid expansion of crops and pastures over vast areas of natural woodlands and savannas. In this paper, we explored the effect of this transformation on vegetation productivity (magnitude, and seasonal and long-term variability) along aridity gradients which span from semiarid to subhumid conditions, considering exclusively those areas with summer rains (>66%). Vegetation productivity was characterized with the proxy metric "Enhanced Vegetation Index" (EVI) (2000 to 2012 period), on 6186 natural and cultivated sampling points on five continents, and combined with a global climatology database by means of additive models for quantile regressions. Globally and regionally, cultivation amplified the seasonal and inter-annual variability of EVI without affecting its magnitude. Natural and cultivated systems maintained a similar and continuous increase of EVI with increasing water availability, yet achieved through contrasting ways. In natural systems, the productivity peak and the growing season length displayed concurrent steady increases with water availability, while in cultivated systems the productivity peak increased from semiarid to dry-subhumid conditions, and stabilized thereafter giving place to an increase in the growing season length towards wetter conditions. Our results help to understand and predict the ecological impacts of deforestation on vegetation productivity, a key ecosystem process linked to a broad range of services.

The imprint of humans on landscape patterns and vegetation functioning in the dry subtropics.

Dry subtropical regions (DST), originally hosting woodlands and savannas, are subject to contrasting human pressures and land uses and different degrees of water limitation. We quantified how this variable context influences landscape pattern and vegetation functioning, by exploring the associations between three groups of variables describing (i) human pressures (population density, poverty, and market isolation) and climate (water availability), (ii) landscape pattern (woody cover, infrastructure, paddock size, etc.), and (iii) vegetation functioning (magnitude and stability of primary productivity), in regions of Asia, Africa, Australia, and America. We collected data from global socioeconomic databases and remote sensing products for 4525 samples (representing uncultivated and cultivated conditions), located along 35 transects spanning semiarid to subhumid conditions. A Reciprocal Averaging ordination of uncultivated samples revealed a dominant gradient of declining woody cover accompanied by lower and less stable productivity. This gradient, likely capturing increasing vegetation degradation, had a negative relationship with poverty (characterized by infant mortality) and with market isolation (measured by travel time to large cities). With partial overlaps, regions displayed an increasing degradation ranking from Africa to South America, to Australia, to North America, and to Asia. A similar analysis of cultivated samples, showed a dominant gradient of increasing paddock size accompanied by decreasing primary productivity stability, which included all regions except Asia. This gradient was negatively associated with poverty and population density. A unique combination of small paddocks and high infrastructure differentiated Asian cultivated samples. While water availability gradients were related to productivity trends, they were unrelated to landscape pattern. Our comparative approach suggests that, in DST, human pressures have an overwhelming role driving landscape patterns and one shared with water availability shaping vegetation functioning.

La estimación de la superficie agrícola: antecedentes y una propuesta metodológica / Croped aerea estimates: background and methodological proposal

Numerosos actores socioeconómicos y políticos utilizan las estimaciones de la superficie cultivada para planificar, reducir la incertidumbere o mejorar la asiganción de recursos. Para resultar confiables y útiles, las estimaciones deben basarse en una metodología debidamente documentada, reproducible para el espacio y en el tiempo, idependiente del observador, evaluable de manera cuantitativa. ¿En qué medida se satisfacen los criterio anteriores en Argentina?. Más allá de su utilidad, la información disponible incorpora fuentes de incertidumbre que afectan seriamente las estimaciones. Estas icluyen las dificulatdes para referir las estimaciones a un área determinada, las posibilidades de los informantes de integrar la información local, la ausencia de protocolos claros y diferencias asociadas a la heterogeneidad de formación, motivación y compromiso de los informantes. La comparación de las estimaciones en dos agencias independientes para una año particular arroja, para las mismas áreas, diferencias de hasta el 24 por ciento en el área sembrada con trigo. Esta diferencia es muy superior a las variaciones interanuales que pretenden detectarse. El análisis multiespectral y multitemporal de imágenes satelitales permite discriminar tipos de cobertura del suelo sobre la base de su comportamiento fenológico. La combinación de información satelital provenientes de sensores con distinta resolución espacial ofrece enormes posibilidades para descripción de los tipos de cobertura del suelo y la estimación de superficies agrícolas. En tal sentido se presenta una propuesta operativa, basada en el uso de imágenes Landsat TM, SAC-C y AVHRR/NOAA, para la evolución regional de la superficie cultivada en Mercosur