RESUMEN
Antimicrobial and probiotic properties were sought in Tunisian raw camel milk. One hundred and forty strains were isolated for this purpose. Initially, a screening based on growth kinetics under gastrointestinal tract (GIT) conditions allowed us to select only one strain that showed good resistance to pH 3 and 5% bovine bile. This isolate named LC38 was assayed for its autoaggregation capacity, surface hydrophobicity, antibiotic sensitivity, haemolytic and antimicrobial activities. The findings revealed strain LC38 sensitivity to several antibiotics, no haemolytic activity and antimicrobial activities against six pathogenic bacteria with an inhibition diameter that varies between 28 and 43 mm. Furthermore, this study revealed that this strain had good autoaggregation characteristics after 18 h of incubation and a high surface hydrophobicity that enhanced its adhesion ability to epithelial cells and for biofilm formation. Strain LC38 was submitted to classical identification with API50CH and to 16S rRNA gene sequencing, which revealed that the strain could be allocated to Lactiplantibacillus plantarum. Analysis of the structural composition of strain LC38 by high-performance liquid chromatography (HPLC) and Fourier transform-infrared (FTIR) spectroscopy analysis showed that probiotic suspension contains organic acids (lactic acid, succinic acid and citric acids). The application of Lactiplantibacillus plantarum LC38 on wound site in a diabetic rat model enhanced significantly wound healing activity and accelerated the wound closure after 14 days of wound induction. Altogether, these results demonstrated that LC38 isolate from camel milk has a number of promising properties that make it a prominent candidate with strong wound healing potential presumably achieved through its antimicrobial activities.
Asunto(s)
Diabetes Mellitus Experimental , Probióticos , Animales , Camelus , Bovinos , Leche , ARN Ribosómico 16S/genética , Ratas , Ratas Wistar , Cicatrización de HeridasRESUMEN
Previous studies have brought to light the toxic effect of cerium chloride (CeCl3) but very little is known about the oxidative brain injury caused by this metal. Medical plants have a well-recognized role in the management of damage caused by pollutants such as CeCl3. Syzygium aromaticum, a potent natural source of bioactive compounds and rich in secondary metabolites, has a broad range of biological functions. The aim of this study is to investigate the capacity of Syzygium aromaticum ethanol extract (ESA) to improve the adverse effects of CeCl3 in the brain tissue. Adult mice were exposed to CeCl3 (20 mg/kg body weight [BW]), with or without ESA, for 60 days. We investigate mice's behavior, damages of cholinergic system and oxidative stress parameters in mice brain. In the present study, in vitro test confirmed that ESA has antioxidant capacity attributed to the presence of flavonoids, polyphenols, and tannins contents. In vivo study showed that CeCl3 caused brain injuries manifested in memory impairment, increase in acetylcholinesterase activity, oxidative stress biomarkers (lipid, proteins, enzymatic and non-enzymatic antioxidant systems), and histopathological alteration in brain tissue. Addition of ESA repaired memory impairment, decreased acetylcholinesterase activity, restored oxidative state, and prevented histopathological alteration. In conclusion, the experimental results showed the protective effects of ethanol extract of Syzygium aromaticum against cerium-induced brain damage.
Asunto(s)
Encéfalo/efectos de los fármacos , Cerio/toxicidad , Fármacos Neuroprotectores/uso terapéutico , Síndromes de Neurotoxicidad/tratamiento farmacológico , Extractos Vegetales/uso terapéutico , Syzygium/química , Animales , Antioxidantes/metabolismo , Encéfalo/metabolismo , Ratones , Fármacos Neuroprotectores/aislamiento & purificación , Síndromes de Neurotoxicidad/etiología , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/aislamiento & purificaciónRESUMEN
CONTEXT: Since antiquity, Pistacia lentiscus L. (Anacardiaceae) fruit oil (PLFO) has been used as a remedy for primary health care such as burn treatment. OBJECTIVE: This study assesses the healing effect of PLFO on CO2 laser fractional burn in a rat model. MATERIALS AND METHODS: The study was carried out on 18 adult male Wistar rats. A second-degree laser burn (wound area = 2.2 cm2) was inflicted in the dorsal region by the application of CO2 fractional laser within the following parameters; Energy level: 25 MJ and Depth level: 4. After applying laser, the rats were divided into three groups: the first was treated with saline solution, the second with a reference cream 'CYTOL BASIC®' (0.13 µg/mm2) and the third with PLFO (0.52 µL/mm2). All treatments were topically administered for eight days. The healing effect was assessed using macroscopic, histological and biochemical parameters. RESULTS: After eight days, the higher percentage of wound healing contraction was observed among the PLFO-treated group (100%) followed by the 'CYTOL BASIC®' treated group (61.36%) and untreated group (32.27%). During the treatment, the PLFO-treated group showed less erythema, less crusting/scabbing, higher general wound appearance scores and a high content of collagen (220.67 ± 7.48 mg/g of tissue) than the other groups. DISCUSSION AND CONCLUSION: The current study has shown, for the first time, the healing effect of PLFO on CO2 laser fractional burn. Their wound healing effect could be attributed to their antimicrobial, anti-inflammatory and antioxidant effects.
Asunto(s)
Quemaduras/tratamiento farmacológico , Fármacos Dermatológicos/farmacología , Frutas/química , Láseres de Gas , Pistacia/química , Extractos Vegetales/farmacología , Aceites de Plantas/farmacología , Piel/efectos de los fármacos , Cicatrización de Heridas/efectos de los fármacos , Animales , Antiinfecciosos/aislamiento & purificación , Antiinfecciosos/farmacología , Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/farmacología , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Quemaduras/etiología , Quemaduras/metabolismo , Quemaduras/patología , Colágeno/metabolismo , Fármacos Dermatológicos/aislamiento & purificación , Modelos Animales de Enfermedad , Hidroxiprolina/metabolismo , Masculino , Fitoterapia , Extractos Vegetales/aislamiento & purificación , Aceites de Plantas/aislamiento & purificación , Plantas Medicinales , Ratas Wistar , Piel/metabolismo , Piel/patología , Factores de TiempoRESUMEN
BACKGROUND: Despite the achievements in modern medicine, there is no effective natural treatment of second-degree burns till now. Therefore, the purpose of this study was to assess the wound-healing efficiency of three extracted oils, from the prickly pear, pumpkin and linseed on partial-thickness burns induced by fractional CO2 laser (an innovative up-to-date technique) in rats. MATERIALS AND METHODS: Thirty rats completing partial-thickness burns by fractional CO2 laser were randomly divided into five groups. The burns were measured, photographed, and topically treated daily with saline solution, "CYTOL BASIC," pumpkin-, linseed-, and prickly pear-extracted oils (0.52 µL/mm(2) of oil) until day 7. The response to treatments was assessed by macroscopic, histologic, and biochemical parameters. RESULTS: Due to their chemical composition, the extracted oils showed significant improvements over the control and reference groups during the trial for both general wound appearance and crusting. On day 7, the prickly pear, linseed, and pumpkin oils showed a significant decrease in the healing time (0 cm(2), 0.15 cm(2), and 0.18 cm(2), respectively) when compared to the control and reference groups (1.49 cm(2) and 0.85 cm(2)). Histologic assessment of the prickly pear oil-treated group revealed good healing proprieties compared with the other groups. The collagen content in prickly pear oil-treated group was found to be significantly greater (270.67 ± 7.48) than that in all other groups. CONCLUSIONS: Our experiment has shown, for the first time, a scientific evidence of the efficiency of extracted oils of prickly pear, pumpkin, and linseed on partial-thickness burns.
Asunto(s)
Quemaduras/tratamiento farmacológico , Magnoliopsida , Fitoterapia , Aceites de Plantas/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos , Animales , Peso Corporal , Quemaduras/etiología , Quemaduras/patología , Colágeno/metabolismo , Cucurbita , Evaluación Preclínica de Medicamentos , Lino , Láseres de Gas/efectos adversos , Masculino , Opuntia , Aceites de Plantas/farmacología , Distribución Aleatoria , Ratas Wistar , Piel/metabolismo , Piel/patologíaRESUMEN
BACKGROUND: Increasing natural drug demand for pharmaceutical uses has encouraged scientifics all over the world to explore medicinal plants recognized as efficient remedies. In this context, extracted oil from pumpkin seeds (Cucurbita pepo L.) is an interesting target, as it is composed with prominent pharmacological properties to possible wound healing treatments. METHODS: The composition and content of certain bioactive constituents of the cold pressed oil obtained from pumpkin seeds (Cucurbita pepo L.) were analyzed and studied for their wound healing properties. Uniform wounds were induced on the dorsum of 18 rats, randomly divided into three groups. The wounds were photographed, and topically treated with saline solution (control group), 0.13 mg/mm(2) of a reference drug ("Cicaflora cream®"), and 0.52 µl/mm(2) of pumpkin's oil each 2 days until the first group is completely healing and so far biopsies were histologically assessed. RESULTS: The composition and content of tocopherols, fatty acids, and phytosterols were determined. The results showed an excellent quality of pumpkin oil with high content of polyunsaturated fatty acids (Linoleic acid: 50.88 ± 0.106 g/100 g of total fatty acids), tocopherols (280 ppm) and sterols (2086.5 ± 19.092 ppm). High content of these bioactive components were in agreement with an efficient wound healing by the mean of an in vivo study. In fact, morphometric assessment and histological findings revealed healed biopsies from pumpkin oil treated group of rats, unlike untreated group, and a full re-epithelialization with reappearance of skin appendages and well organized collagen fibers without inflammatory cells. CONCLUSIONS: This study showed the significance of oil from pumpkin seeds (Cucurbita pepo L.) as a promising drug to healing wounds in animal assays. As a whole, pumpkin's oil would be recommended in the nutritional and medicinal purposes.
Asunto(s)
Cucurbita/química , Evaluación Preclínica de Medicamentos/métodos , Aceites de Plantas/farmacología , Cicatrización de Heridas/efectos de los fármacos , Animales , Antioxidantes/química , Antioxidantes/farmacología , Ácidos Grasos/análisis , Células HeLa , Humanos , Masculino , Pruebas de Sensibilidad Microbiana , Fitosteroles/análisis , Fitosteroles/química , Aceites de Plantas/química , Ratas Wistar , Semillas/química , Tocoferoles/análisis , Triglicéridos/análisisRESUMEN
CONTEXT: Medicinal plants have been recognized as useful remedies for primary health care. Accordingly, Cucurbita pepo L. (Cucurbitaceae) (pumpkin) and Linum usitatissimum (L.) Griesb. (Linaceae) (linseed) which have extracted oil with prominent pharmacological properties are investigated as possible burn healing treatments. OBJECTIVE: The present study assesses the healing potential of pumpkin and linseed extracted oils on rats. MATERIALS AND METHODS: Uniform deep second-degree burns were induced on the dorsum of 24 rats, randomly divided into four groups. The burns were measured, photographed, and topically treated with saline solution, "Cytol Centella®", pumpkin, and linseed-extracted oils (0.52 µl/mm(2) of oil) each 2 d (up until day 33). Post-burning of the 33rd day, biopsies were histologically assessed. RESULTS: At the end of the experiment, the rat groups treated with linseed, pumpkin oils, and "Cytol Centella®" had higher percentage of wound contraction (98.68, 96.71, and 92.54%, respectively) than the control group (58.38%). Wound biopsies from rats treated with extracted oils showed the best tissue regeneration proprieties as compared with the other groups. The histomorphometric analysis of biopsies revealed that linseed oil could significantly stimulate angiogenesis (55.6% ± 7.25). The pumpkin oil, and Cytol Centella® could significantly increase the collagen production 64.9% ± 5.94, and 61.2% ± 7.36, respectively. DISCUSSION AND CONCLUSION: Overall, our study has given for the first time scientific evidence of the healing efficiency of pumpkin and linseed oils on burn-wounds.
Asunto(s)
Quemaduras/tratamiento farmacológico , Cucurbita , Lino , Aceite de Linaza/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos , Animales , Quemaduras/patología , Evaluación Preclínica de Medicamentos/métodos , Aceite de Linaza/aislamiento & purificación , Aceite de Linaza/farmacología , Masculino , Aceites de Plantas/aislamiento & purificación , Aceites de Plantas/farmacología , Aceites de Plantas/uso terapéutico , Ratas , Ratas Wistar , Semillas , Cicatrización de Heridas/fisiologíaRESUMEN
Almond gum is a naturally occurring polymer produced by almond trees and shrubs. Its abundance, as well as its low cost production makes it a potential feedstock for use in food and pharmaceuticals. In this regard, almond gum oligosaccharides were enzymatically generated, purified and their monosaccharide composition assessed using gas chromatography-flame ionization detector. Oligosaccharide analyses show that the most prominent residues were galactose and arabinose with traces of xylose, rhamnose, glucose and mannose. The glycosyl linkage positions were analyzed using gas chromatography - mass spectrometry showing a main chain composed of galactose units [â3)-Gal-(1â] branched mainly with arabinose residues [Ara-(1â]. The potent role of the generated oligosaccharides on rats wound healing was investigated. They have been applied either alone or supplemented, as active substance, with cream formulation, on full-thickness wound created on the dorsum of the rats. The effect of oligosaccharides was assessed by measuring the wound closure percentage, reaching an average of around 100% when applied alone or supplemented to cream formulation. The healing percentage for the control group was only 74.3% at the same day. The histological evaluation of skin sections visualized by light microscopy revealed an improved collagen deposition and an increased fibroblast and vascular densities.
Asunto(s)
Oligosacáridos/uso terapéutico , Prunus dulcis/química , Piel/lesiones , Cicatrización de Heridas/efectos de los fármacos , Envejecimiento , Animales , Cromatografía , Oligosacáridos/aislamiento & purificación , Oligosacáridos/farmacología , Ratas , Ratas WistarRESUMEN
The present study undertakes the extraction of a novel polysaccharide from Lepidium sativum (PLS) and the determination of its physicochemical composition and antioxidant properties, as well as its potential wound healing activity in alloxan-induced diabetic rats. This polysaccharide presented a lighter natural color, whose luminosity (L∗), red-green intensity (a∗), and blue-yellow intensity (b∗) were recorded at 63.26, 5.87, and 27.28, respectively. The PLS was structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, UV spectrum, high performance liquid chromatography (HPLC), gas chromatography (GC), nuclear resonance magnetic (NMR), and high-pressure gel filtration chromatography. The FT-IR and UV spectra showed the characteristic band of polysaccharides. According to HPLC, the crude PLS is a heteropolysaccharide composed of glucose, xylose, and galactose. Results obtained by 1H NMR indicated that PLS consisted of three monosaccharide residues with α and ß anomers. This novel polysaccharide had an average molecular weight of 98.51 kDa and displayed potential antioxidant activities determined through three different assays: scavenging activity against 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, and reducing power. These results strongly support the beneficial effects of the PLS to accelerate wound healing in diabetic rats. Indeed, its application significantly increased wound contraction percentage (98 ± 1.11%) after 14 days of experiment. Furthermore, the histological assessment of the PLS-treated group demonstrated complete reepithelialized wounds by accelerating collagen synthesis. In general, the findings affirmed that PLS is efficient on wound closure in alloxan-induced diabetic rats.
Asunto(s)
Antioxidantes , Diabetes Mellitus Experimental , Aloxano/farmacología , Animales , Antioxidantes/química , Antioxidantes/farmacología , Lepidium sativum , Polisacáridos/química , Polisacáridos/farmacología , Ratas , Espectroscopía Infrarroja por Transformada de Fourier , Cicatrización de HeridasRESUMEN
Despite advances in diabetes care, impaired diabetic wound healing remains a significant clinical problem. The present study was aimed at developing a novel cream based on Ginkgo biloba extract and investigating its wound healing effect on full-thickness wounds in diabetic rats. The topical formulated oil-in-water emulsion-based cream contains Ginkgo biloba aqueous extract in an amount of about 1% to 5% as an active agent. The prepared formula was subjected to physicochemical assessment and pharmacotechnical characterization. Eighteen alloxan-induced diabetic rats completing full-thickness excisional skin wounds were randomly divided into three groups topically treated with either a normal saline (control group), the reference drug ("Cytol Centella cream®"), and cream based on the Ginkgo biloba extract. The response to treatment was assessed by macroscopic, qualitative, and quantitative histopathological analysis. The prepared formula showed good physicochemical properties. The rheological behavior of the prepared cream followed a non-Newtonian pseudoplastic pattern at different storage temperatures. The cream, which is a macroemulsion with uniform size distribution, remained stable for 6 months. Skin tolerance studies confirmed the compatibility of the cream with the skin. During the experimental trial, the cream based on the Ginkgo biloba-treated group showed significant improvements over the control and reference groups for both general wound appearance and healing dynamics. This increased rate of closure of wounds in diabetic rats was associated with increased collagen synthesis. Our findings showed that the cream could be a promising and innovative topical treatment with Ginkgo biloba extract for the management of acute diabetic wounds.
Asunto(s)
Diabetes Mellitus Experimental/tratamiento farmacológico , Ginkgo biloba/química , Extractos Vegetales/farmacología , Crema para la Piel/farmacología , Piel/efectos de los fármacos , Cicatrización de Heridas/efectos de los fármacos , Administración Tópica , Animales , Diabetes Mellitus Experimental/complicaciones , Diabetes Mellitus Experimental/patología , Modelos Animales de Enfermedad , Masculino , Ratas , Ratas Wistar , Piel/patologíaRESUMEN
The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model. LWSP is a heteropolysaccharide that consists of glucose, mannose, xylose and arabinose. Three different tests were used to evaluate the antioxidant activity of this carbohydrate. The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5â¯mg/ml was 99.77%. The total antioxidant capacity of LWSP at 12â¯mg/ml was equivalent to 166.61⯵g acsorbic acid. LWSP displayed a high protection effect against DNA damage induced byhydroxyl radical. No hemolytic activity was observed towards human erythrocytes. LWSP was tested in functional properties. The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively). In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8â¯days of injury. According to the histological assessment, the LWSP-treated group had a higher content of hydroxyproline (846. 67⯱â¯92.28â¯mg/g tissue) than the other groups. Overall, the findings demonstrate that the application of this novel LWSP may open promising opportunities for burn wound healing in modern medicine.
Asunto(s)
Antioxidantes/química , Quemaduras/tratamiento farmacológico , Lino/química , Extractos Vegetales/química , Polisacáridos/química , Semillas/química , Cicatrización de Heridas/efectos de los fármacos , Animales , Antioxidantes/farmacología , Benzotiazoles/química , Línea Celular , Emulsiones/química , Eritrocitos/citología , Depuradores de Radicales Libres/metabolismo , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Hidroxiprolina/metabolismo , Rayos Láser , Masculino , Extractos Vegetales/farmacología , Polisacáridos/farmacología , Ratas Wistar , Ácidos Sulfónicos/químicaRESUMEN
This work was conducted to evaluate the compatibility between physicochemical, antioxidant and morphological properties of polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa reinforced by poly (vinyl alcohol) (PVA) composed films at different ratios of FRP/PVA: F1 (70:30), F2 (50:50), F3 (30:70) and PVA (100% PVA) and the potential wound healing effects. As assessed, FRP/PVA prepared films were heterogeneous, slightly opaque with a rough surface as ascertained by Fourier transform infrared spectroscopy, scanning electron microscopy and colorimetric parameters. Even, X-ray diffraction and glass transition results revealed a semi-crystalline structure of FRP composed films which decreased with increasing PVA ratios. The antioxidant activities of composite films depicted that F1 exhibited the highest antioxidant activity in vitro. Therefore, F1 was found to promote significantly the wound healing, after eight days of treatment, evidenced by higher wound appearance scores and a higher content of collagen (885.12⯱â¯20.35â¯mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL BASIC® and PVA-treated groups. All together, the marine-derived polysaccharide gave a substantial pledge for the development of biodegradable films as a potent antioxidant material and a promising agent for tissue regeneration.
Asunto(s)
Quemaduras/fisiopatología , Rayos Láser/efectos adversos , Polisacáridos/química , Polisacáridos/farmacología , Alcohol Polivinílico/química , Rhodophyta/química , Cicatrización de Heridas/efectos de los fármacos , Animales , Antioxidantes/química , Antioxidantes/farmacología , Antioxidantes/uso terapéutico , Quemaduras/etiología , Quemaduras/metabolismo , Quemaduras/patología , Colágeno/metabolismo , Femenino , Hidroxiprolina/metabolismo , Polisacáridos/uso terapéutico , Ratas , Ratas Wistar , SolubilidadRESUMEN
This work aimed to the development of chitosan and protein isolate composite hydrogels, for carotenoids-controlled delivery and wound healing. By increasing the concentration of the protein isolate, chitosan hydrogels were more elastic at a protein isolate concentration not exceeding 15% (w/w). Chitosan-protein isolate composite hydrogels revealed low cytotoxicity towards MG-63 osteosarcoma cells. Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study. Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0). Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities. The in vivo healing potential of hydrogels in rats' models was further studied. Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.
Asunto(s)
Materiales Biocompatibles/farmacología , Braquiuros/metabolismo , Carotenoides/química , Quitosano/química , Hidrogeles/química , Proteínas de Mariscos/química , Cicatrización de Heridas/efectos de los fármacos , Animales , Antioxidantes/química , Materiales Biocompatibles/química , Carotenoides/metabolismo , Carotenoides/farmacología , Línea Celular , Supervivencia Celular/efectos de los fármacos , Liberación de Fármacos , Humanos , Hidrogeles/metabolismo , Concentración de Iones de Hidrógeno , Ratas , Ratas Wistar , Solubilidad , TemperaturaRESUMEN
Medicinal plants have been used as a source of effective and safe alternative therapeutic agents for various ailments including inflammation. In fact, the aim of this study is to assess the topical anti-inflammatory and antioxidative potential effects of Cucurbita pepo (pumpkin), Linum usitatissimum (linseed), and Opuntia ficus indica (prickly pear) oils on acute inflammation using carrageenan-induced paw edema model. The study was conducted on 36 rats splitted in 6 groups: a normal control group and 5 carrageenan-treated groups (1%), each treated with either a normal saline, the reference drug ("Inflocine®" 2 mg/paw), pumpkin, linseed, or prickly pear oils (25 µl/paw). The response to these treatments was mainly assessed by the measuring of edema paw size, hematological and biochemical analysis, oxidative stress testing, and histological study. All the studied seed oils especially prickly pear oil proved to be efficient in treating acute inflammation. The oil-treated groups revealed a significant (p < 0.05) decrease in the clinical signs of inflammation, hematological parameters (white blood cells and platelets), concentrations of CRP and fibrinogen, and congestion compared to the normal saline-treated group. The results also showed that the tested oils, endowed with a radical scavenging ability, could significantly increase the activities of SOD, CAT, and GPx in carrageenan-treated skin by reducing the lipid peroxidation and protein oxidation (TBARS, AOPP). The anti-inflammatory effect of the tested oils was closely related to both their antioxidant properties as well as their bioactive compounds (polyunsaturated fatty acids, vitamin E, and phytosterols). For the first time, the findings of the current study highlight the "in vivo" anti-inflammatory property of pumpkin, linseed, and prickly pear oils on carrageenan-induced acute inflammation by regulating inflammatory mediators and oxidative stress markers.
Asunto(s)
Antiinflamatorios/farmacología , Antioxidantes/farmacología , Cucurbita/química , Lino/química , Aceite de Linaza , Opuntia/química , Estrés Oxidativo/efectos de los fármacos , Enfermedad Aguda , Animales , Antiinflamatorios/química , Antioxidantes/química , Biomarcadores/metabolismo , Inflamación/tratamiento farmacológico , Inflamación/metabolismo , Inflamación/patología , Aceite de Linaza/química , Aceite de Linaza/farmacología , Masculino , Ratas , Ratas WistarRESUMEN
This work was undertaken to determine the structural characteristics of a novel polysaccharide, Sorghum Water-Soluble Polysaccharide (SWSP), extracted from Sorghum (Sorghum bicolor (L.)) seeds and to assess its in vitro hemolytic and antioxidant activities as well as its in vivo wound healing ability to treat burns induced by fractional CO2 laser. Results obtained by 13C, 1H and HSQC Nuclear magnetic resonance (NMR) indicated that SWSP showed the characteristic bands of polysaccharides. Scanning electron microscopy of SWSP showed a rough surface with numerous cavities. SWSP exhibited strong antioxidant activities determined through three different assays: ferrous chelating activity, reducing power and DNA nicking. Also, no hemolytic activity was detected towards human erythrocytes. On the other hand, the application of SWSP hydrogel on the burn site in a rat model significantly improved wound appearance and accelerated the wound closure after 8â¯days of induction. Histological assessment of biopsies also demonstrated complete re-epithelialized wounds with a full epidermal regeneration. Thus, SWSP hydrogel which was efficient on wound closure might be useful as a wound healing agent in modern medicine.
Asunto(s)
Quemaduras/fisiopatología , Hemólisis/efectos de los fármacos , Rayos Láser/efectos adversos , Polisacáridos/química , Polisacáridos/farmacología , Sorghum/química , Cicatrización de Heridas/efectos de los fármacos , Animales , Antioxidantes/química , Antioxidantes/farmacología , Quemaduras/etiología , Quemaduras/metabolismo , Quemaduras/patología , Hidroxiprolina/metabolismo , Ratas , Ratas Wistar , Semillas/químicaRESUMEN
OBJECTIVE: The aim of this study was to investigate the chemical composition and antioxidant properties of Urtica urens L.(Uu) as well as its anti-inflammatory effect on carrageenan (CARR)-induced paw oedema in rats. METHODS: The leaves were extracted using ethanol (EtOH) and water. The extracts were analysed for proximate composition and antioxidant activity using standard chemical analysis methods. RESULTS: The proximate analysis showed that Uu leaves contained appreciable percentages of dry mass, ashes, carbohydrates, proteins, lipids, sugars, anthocyanin, carotenoid and fibre. Results showed that EtOH extract contained the highest amount of total phenolics, flavonoids, tannins, ortho-diphenols and flavonols. It decreased the paw oedema after CARR administration, and ameliorated the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and the malondialdehyde (MDA). CONCLUSIONS: Uu displayed a high potential as a natural source of minerals, phytochemicals and antioxidant properties. EtOH extract exhibited a significant inhibition against CARR-induced inflammation.
Asunto(s)
Antiinflamatorios/farmacología , Antioxidantes/farmacología , Edema/tratamiento farmacológico , Fitoquímicos/análisis , Fitoterapia , Extractos Vegetales/farmacología , Hojas de la Planta/química , Urticaceae/química , Animales , Carragenina/toxicidad , Cromatografía Liquida , Edema/inducido químicamente , Masculino , Malondialdehído/metabolismo , Estrés Oxidativo/efectos de los fármacos , Ratas , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
The present study aimed to evaluate the in vitro antibacterial and antioxidant activities and the in vivo wound healing performance of a noval exopolysaccharides (EPS-Ca6) produced by Lactobacillus sp.Ca6 strain. The results showed that EPS-Ca6 had a potential antioxidant activity determined through four different assays: DPPH scavenging activity, reducing power, ß-carotene bleaching by linoleic acid assay, and Metal chelating activities. It also exhibited significant antibacterial activity against pathogenic bacteria Salmonella enterica and Micrococcus luteus. The wound healing activity of the EPS-Ca6, using excision wound model in rats, showed that this novel EPS accelerated significantly wound healing activity as compared to the control group, and a total closure was achieved after 14days of wound induction. Furthermore, histological examination of biopsies showed fully re-epithelialized wound with a complete epidermal regeneration. Overall the finding indicates that the EPS-Ca6 might be useful as a wound healing agent in modern medicine.
Asunto(s)
Antibacterianos/farmacología , Antioxidantes/farmacología , Quelantes/farmacología , Lactobacillus/química , Polisacáridos Bacterianos/farmacología , Piel/efectos de los fármacos , Cicatrización de Heridas/efectos de los fármacos , Animales , Peso Corporal/efectos de los fármacos , Hidroxiprolina/metabolismo , Masculino , Ratas , Ratas Wistar , Piel/metabolismoRESUMEN
The present paper investigated the efficiency of Urtica dioica (U. dioica) on hemostatic and wound healing activities. U. dioica leaf extracts were evaluated for their antibacterial and antioxidant effects as well as their flavonoid and polyphenol content. The hydroethanolic extract (EtOH-H2OE), showing the most potent antibacterial and antioxidant activities in vitro, thanks to its flavonoid and polyphenol richness, was selected for hemostatic and wound healing evaluation. Twenty-four rats completing full-thickness wounds were split into four groups. The wounds were topically treated with saline solution, glycerol, "CICAFLORA," and U. dioica EtOH-H2OE (50 µL/mm2) until day 11. The wound healing effect was assessed by macroscopic, histological, and biochemical parameters. Rats treated with EtOH-H2OE showed fast wound closure (92.39%) compared to the control animals (60.91%) on the 11th day of wounding (P < 0.01). Histopathological and biochemical explorations showed full epidermal regeneration and an improvement of the hydroxyproline content in the U. dioica EtOH-H2OE treated rats. Analysis of fatty acids and sterols by GC-MS showed the presence of unsaturated fatty acids and a high concentration of lupeol known for their involvement in reepithelialization. These results prove the efficiency of U. dioica EtOH-H2OE in wound healing and supported its traditional use.
Asunto(s)
Extractos Vegetales/administración & dosificación , Urtica dioica/química , Cicatrización de Heridas/efectos de los fármacos , Heridas y Lesiones/tratamiento farmacológico , Administración Tópica , Animales , Antibacterianos , Antioxidantes/administración & dosificación , Antioxidantes/química , Escherichia coli/efectos de los fármacos , Escherichia coli/patogenicidad , Flavonoides/química , Hemostáticos/administración & dosificación , Hemostáticos/química , Humanos , Extractos Vegetales/química , Hojas de la Planta/química , Polifenoles/química , Ratas , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/patogenicidad , Heridas y Lesiones/microbiología , Heridas y Lesiones/patologíaRESUMEN
The aim of this work was to evaluate the antioxidant and hemolytic activities as well as the in vivo wound healing performance of a novel polysaccharide (FWEP) extracted from fenugreek (Trigonella foenum-graecum) seeds. The antioxidant activity was evaluated in vivo and in vitro using various assays. Results showed that FWEP exhibited strong antioxidant activities but no hemolytic activity was observed towards bovine erythrocytes. The application of FWEP hydrogel on the wound site in a rat model enhanced significantly wound healing activity and accelerated the wound closure after 14days of wound induction. Histological examination also demonstrated fully re-epithelialized wound with a complete epidermal regeneration. Altogether, these evidences demonstrated that FWEP had strong wound healing potential presumably achieved through its antioxidant activities.
Asunto(s)
Antioxidantes/farmacología , Hemólisis/efectos de los fármacos , Polisacáridos/farmacología , Semillas/química , Piel/efectos de los fármacos , Trigonella/química , Cicatrización de Heridas/efectos de los fármacos , Animales , Antioxidantes/química , Biomarcadores/metabolismo , Compuestos de Bifenilo/química , Tiempo de Sangría , Peso Corporal/efectos de los fármacos , Peróxido de Hidrógeno/metabolismo , Hidroxiprolina/metabolismo , Hierro/química , Masculino , Oxidación-Reducción/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Picratos/química , Polisacáridos/química , Ratas , Ratas Wistar , Piel/metabolismo , Fenómenos Fisiológicos de la Piel/efectos de los fármacos , beta Caroteno/metabolismoRESUMEN
In order to find new topical anti-inflammatory agents, we had recourse to a medicinal plant. This work was designed to determine the topical anti-inflammatory effect of Pistacia lentiscus fruit oil (PLFO), using carrageenan-induced paw edema rat model, and to evaluate its effects on oxidative stress. The topical anti-inflammatory activity of PLFO was compared to Inflocine® and estimated by measuring the diameter of paw edema, for 5 hours at a 1-hour interval. After that the rats were scarified and the inflamed paw tissue was removed for the exploration of some parameters of oxidative stress and histopathology. PLFO showed a significant anti-inflammatory activity in comparison with the Inflocine. The percentages of edema inhibition were 70% and % 51.5% (p < 0.01), respectively, after five hours. The treatment with PLFO and Inflocine led to significant increases (p ≤ 0.05) in the activities of CAT, SOD, and GPX and significant decreases in the MDA level and AOPP activity in the paw tissue after Carr injection, in comparison with the Carr group. Therefore, our findings demonstrate that PLFO might accelerate the development of new drugs which could be used scientifically as a source for natural health products in the treatment of topical inflammation.
RESUMEN
The sap of the date palm "Lagmi" is a clear liquid, rich in sugars and minerals, with a pleasant flavour. Folk remedies based on the use of "Lagmi" for wound healing are still practiced. However, no studies investigated the relevance of "Lagmi" for wound healing. Therefore, the aim of this study was to identify the in vivo healing properties of "lagmi" on mechanically wounded wistar rats. Injured rats were divided into three groups: a first group treated by "lagmi", a second reference group processed by CICAFLORA(®) and a third untreated control group. On the 12th day of the experiment, total healing in the first group was reached, while healing was incomplete in the other groups. The sap seems to accelerate cell proliferation and contribute to faster healing with a gain of more than 30% as compared to CICAFLORA(®). Chemical Analysis of "Lagmi" showed important radical scavenging activity and high total antioxidant capacity. Features reported to help healing process and/or provides a favourable environment for tissue healing in wound sites. Extensive characterization of "Lagmi" phenolic and flavonoid compounds by High Resolution LC-MS (LC-HRESIMS) analysis indicates "Lagmi" is an important source of known anti-inflammatory compounds as well as promising wound healing candidates.