c-Jun expression after cerebral hyperstimulation differs between rats and marmosets.

Immediate early genes (IEGs) are a fundamental element in the way we respond and adapt to a variety of stimuli. We have recently reported that IEG response, as measured by c-Fos expression, is different between rodents and primates. Here, we further extend this analysis by assessing the expression of c-Jun, one of the main complements of c-Fos, under the same stimulation protocol. For this, we investigated the immunohistochemical expression of c-Jun (and compared with that previously shown for c-Fos) after stimulation with pentylenetetrazol in the cingulate gyrus, motor cortex, piriform cortex, inferior temporal cortex, and visual cortex of rats and marmosets (Callithrix jacchus), both male and female. Overall the immunohistochemical expression of c-Jun was more intense but remained elevated for a shorter duration in marmosets as compared to rats. These results are in contrast to what we had previously shown for c-Fos. Furthermore, in terms of the temporal profile, c-Fos and c-Jun expression occurred in a complementary manner in rats-the peak of c-Fos expression coincided with low levels of c-jun expression-and in a superimposed manner in marmosets-the peak of c-Fos expression coincided with the peak of c-Jun expression. Since Fos proteins may form dimers with Jun proteins and together control late gene expressions in the cell nucleus, this different expression profile between primates and rodents may bear meaningful impact for how the nervous system reacts and adapts to stimulation.

Insulin-like growth factor-1 (IGF-1) promotes primordial follicle growth and reduces DNA fragmentation through the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signalling pathway.

We investigated the effects of insulin-like growth factor 1 (IGF-1) on the morphology and follicular activation of ovine preantral follicles cultured in situ and whether the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway is involved in IGF-1 action in the sheep ovary. Ovine ovarian fragments were fixed for histological and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) analyses (fresh control) or cultured in supplemented alpha-minimum essential medium (α-MEM+; control) or α-MEM+ with IGF-1 (1, 10, 50, 100 or 200ngmL-1) for 7 days. Follicles were classified as normal or atretic, primordial or growing and the oocyte and follicle diameters were measured. DNA fragmentation was evaluated by TUNEL assay. Proliferating cell nuclear antigen (PCNA) immunohistochemistry was performed on the fresh control, α-MEM+ and 100ngmL-1 IGF-1 samples. Inhibition of PI3K activity was performed through pretreatment with the PI3K inhibitor LY294002 and phosphorylated AKT (pAKT) expression was analysed after culture in the absence or presence of LY294002. IGF-1 at 100ngmL-1 increased (P<0.05) follicular activation compared with α-MEM+ and decreased TUNEL-positive cells (P<0.05) compared with other treatments. PCNA-positive cells also increased (P<0.05) in 100ngmL-1 IGF-1. LY294002 significantly inhibited follicular activation stimulated by α-MEM+ and 100ngmL-1 IGF-1 and reduced pAKT expression in follicles. Overall, IGF-1 at 100ngmL-1 promoted primordial follicle activation, cell proliferation and reduced DNA fragmentation after in situ culture through the PI3K/AKT pathway.

Plant bZIP transcription factors responsive to pathogens: a review.

Transcription factors of the basic leucine zipper (bZIP) family control important processes in all eukaryotes. In plants, bZIPs are master regulators of many central developmental and physiological processes, including morphogenesis, seed formation, abiotic and biotic stress responses. Modulation of the expression patterns of bZIP genes and changes in their activity often contribute to the activation of various signaling pathways and regulatory networks of different physiological processes. However, most advances in the study of plant bZIP transcription factors are related to their involvement in abiotic stress and development. In contrast, there are few examples of functional research with regard to biotic stress, particularly in the defense against pathogens. In this review, we summarize the recent progress revealing the role of bZIP transcription factors in the biotic stress responses of several plant species, from Arabidopsis to cotton. Moreover, we summarize the interacting partners of bZIP proteins in molecular responses during pathogen attack and the key components of the signal transduction pathways with which they physically interact during plant defense responses. Lastly, we focus on the recent advances regarding research on the functional role of bZIPs in major agricultural cultivars and examine the studies performed in this field.

Higher throughput workflow with sensitive, reliable and automatic quantification of myelination in vitro suitable for drug screening.

Multiple sclerosis (MS) is the most common demyelinating autoimmune disease of the central nervous system (CNS). Immune-mediated myelin and axonal damage that is accompanied by chronic axonal loss causing destruction of the myelin sheaths are hallmarks of MS. While great strides have been made in understanding the molecular underpinnings of re-/myelination, currently no remyelination therapy is available for MS. As myelination is a complex process that is not fully understood, we sought to develop a systematic, reliable, automated and quantitative higher throughput screening method. We aimed to quantitate myelin sheaths in vitro with high sensitivity at the single cell level suitable for testing small compound libraries. To this end, we miniaturised in vitro retinal ganglion cell-oligodendrocyte precursor cell (RGC-OPC) co-cultures into a multi-well plate format. This allowed us to maintain the reciprocal interaction of live axons and oligodendrocytes (OLs) to ensure compact myelin formation. To quantify our co-cultures, we developed a novel computer vision algorithm to precisely measure myelination. We demonstrated efficacy of our system with known pro-differentiating compounds BQ3020 and XAV939 which exhibited robust, efficient, and dose dependent effects on myelination. Through this combination of experimental and technical advances, we have developed a method allowing systematic and reliable testing of remyelinating compound efficacy.

Marmosets have a greater diversity of c-Fos response after hyperstimulation in distinct cortical regions as compared to rats.

Previous evidence indicated a potential mechanism that might support the fact that primates exhibit greater neural integration capacity as a result of the activation of different structures of the central nervous system, as compared to rodents. The current study aimed to provide further evidence to confirm previous findings by analyzing the patterns of c-Fos expression in more neocortical structures of rats and marmosets using a more robust quantitative technique and evaluating a larger number of brain areas. Nineteen Wistar rats and 21 marmosets (Callithrix jacchus) were distributed among control groups (animals without injections) and animals injected with pentylenetetrazol (PTZ) and euthanized at different time points after stimulus. Immunohistochemical detection of c-Fos was quantified using unbiased and efficient stereological cell counting in eight neocortical regions. Marmosets had a c-Fos expression that was notably more widely expressed (5× more cells) and longer lasting (up to 3 hr) than rats. c-Fos expression in rats presented similar patterns of expression according to the function of the brain cortical structures (associative, sensorial, and motor functions), which was not observed for marmosets (in which no clear pattern could be drawn, and a more diverse profile emerged). Our results provide evidence that the marmoset brain has a greater neuronal activation after intense stimulation by means of PTZ and a more complex pattern of brain activation. We speculate that these functional differences may contribute for the understanding of the different neuronal processing capacities of the neocortex in these mammals' orders.

Associations between Cues of Sexual Desire and Sexual Attitudes in Portuguese Women.

Sexuality is defined as a multidimensional experience that involves genital, mental, and bodily components. It is also assumed as a basic condition inherent to the human existence that encourages the search for love, intimacy, sex, and proximity to others. The main objective of this study is to assess the relationship between cues of sexual desire and sexual attitudes in Portuguese women. This is a cross-sectional study with 804 Portuguese women to whom the protocol was applied. It included an informed consent, a sociodemographic questionnaire, a questionnaire related to intimacy, a scale of sexual attitudes, and the scale of cues of sexual desire. The protocol was applied via Google Forms due to the current pandemic situation (COVID-19). Differences were found in sexual attitudes and the cues of sexual desire in terms of sociodemographic characteristics, as well as in terms of women's intimacy. Significant correlations were found between the brief sexual attitudes scale (BSAS) and the cues of sexual desire scale (CSDS). Age, sexual orientation, relation nature, sexual practices, visual proximity cues, erotic explicit cues, and sensory explicit cues explain, altogether, 25% of the total sexual attitudes. Additionally, age, sexual orientation, the relation's nature, sexual practices, visual proximity cues, emotional bonding cues, romantic implicit cues, erotic explicit cues, and sensory explicit cues explain, altogether, 30% of the permissiveness. Sexual attitudes are developed under the influence of sociodemographic variables, variables related to women's intimacy, and cues of sexual desire, which are new data in the study of sexual attitudes and have implications at the level of gender issues.

Corrigendum: Ecophysiological Traits of Invasive C3 Species Calotropis procera to Maintain High Photosynthetic Performance Under High VPD and Low Soil Water Balance in Semi-Arid and Seacoast Zones.

[This corrects the article DOI: 10.3389/fpls.2020.00717.].

Different resource-use strategies of invasive and native woody species from a seasonally dry tropical forest under drought stress and recovery.

Exotic plants in semiarid region have developed strategies for efficient use or capture of resources. They have become invasive and outperform native species. To understand which factors could explain the success of invasive woody species in a semiarid region, several physiological traits were analyzed in young plants of two invasive and two native species exposed to different water availability. Invasive plants showed low leaf construction cost, high phosphorus and nitrogen contents, reduced loss of instantaneous energy use efficiency, and smaller specific leaf area when compared to native species. This strategy led to a higher biomass gain and a high root/shoot ratio in both water treatments. After rehydration, invasive plants showed faster recovery and higher rates of CO2 assimilation. This resilience is fundamental for species in semiarid regions, and also increase uptake of nutrients. Maintaining a high photosynthetic rate, whenever there is water availability is a strategy that increases the performance of the species in relation to biomass gain. The low leaf construction cost and the fast recovery of the photosynthetic metabolism of invasive plants after limiting water resources explains the success of these species, and suggests that their potential may increase under prolonged and severe drought seasons.

Ecophysiological Traits of Invasive C3 Species Calotropis procera to Maintain High Photosynthetic Performance Under High VPD and Low Soil Water Balance in Semi-Arid and Seacoast Zones.

The evergreen C3 plant Calotropis procera is native to arid environments. Thus, it grows under high vapor pressure deficit (VPD), intense light, and severe drought conditions. We measured several ecophysiological traits in C. procera plants growing in semi-arid and seacoast environments to assess the attributes that support its photosynthetic performance under these contrasting conditions. Gas exchange analysis, primary metabolism content, nutrients, the antioxidant system, and leaf anatomy traits were measured under field conditions. In the semi-arid environment, C. procera was exposed to a prolonged drought season with a negative soil water balance during the 2 years of the study. Calotropis procera plants were exposed to a positive soil water balance only in the rainy season in the seacoast environment. The leaves of C. procera showed the same photosynthetic rate under high or low VPD, even in dry seasons with a negative soil water balance. Photosynthetic pigments, leaf sugar content, and the activity of antioxidant enzymes were increased in both places in the dry season. However, the anatomical adjustments were contrasting: while, in the semi-arid environment, mesophyll thickness increased in the driest year, in the seacoast environment, the cuticle thickness and trichome density were increased. The ability to maintain photosynthetic performance through the seasons would be supported by new leaves with different morpho-anatomical traits, with contrasting changes between semi-arid and seacoast environments. Furthermore, our results suggest that an efficient antioxidative system and leaf sugar dynamics can contribute to protecting the photosynthetic machinery even under severe drought.

Root Adaptation via Common Genetic Factors Conditioning Tolerance to Multiple Stresses for Crops Cultivated on Acidic Tropical Soils.

Crop tolerance to multiple abiotic stresses has long been pursued as a Holy Grail in plant breeding efforts that target crop adaptation to tropical soils. On tropical, acidic soils, aluminum (Al) toxicity, low phosphorus (P) availability and drought stress are the major limitations to yield stability. Molecular breeding based on a small suite of pleiotropic genes, particularly those with moderate to major phenotypic effects, could help circumvent the need for complex breeding designs and large population sizes aimed at selecting transgressive progeny accumulating favorable alleles controlling polygenic traits. The underlying question is twofold: do common tolerance mechanisms to Al toxicity, P deficiency and drought exist? And if they do, will they be useful in a plant breeding program that targets stress-prone environments. The selective environments in tropical regions are such that multiple, co-existing regulatory networks may drive the fixation of either distinctly different or a smaller number of pleiotropic abiotic stress tolerance genes. Recent studies suggest that genes contributing to crop adaptation to acidic soils, such as the major Arabidopsis Al tolerance protein, AtALMT1, which encodes an aluminum-activated root malate transporter, may influence both Al tolerance and P acquisition via changes in root system morphology and architecture. However, trans-acting elements such as transcription factors (TFs) may be the best option for pleiotropic control of multiple abiotic stress genes, due to their small and often multiple binding sequences in the genome. One such example is the C2H2-type zinc finger, AtSTOP1, which is a transcriptional regulator of a number of Arabidopsis Al tolerance genes, including AtMATE and AtALMT1, and has been shown to activate AtALMT1, not only in response to Al but also low soil P. The large WRKY family of transcription factors are also known to affect a broad spectrum of phenotypes, some of which are related to acidic soil abiotic stress responses. Hence, we focus here on signaling proteins such as TFs and protein kinases to identify, from the literature, evidence for unifying regulatory networks controlling Al tolerance, P efficiency and, also possibly drought tolerance. Particular emphasis will be given to modification of root system morphology and architecture, which could be an important physiological "hub" leading to crop adaptation to multiple soil-based abiotic stress factors.

Phakopsora pachyrhizi triggers the jasmonate signaling pathway during compatible interaction in soybean and GmbZIP89 plays a role of major component in the pathway.

The biotrophic fungus Phakopsora pachyrhizi is currently the major pathogen affecting soybean production worldwide. It has already been suggested for the non-host interaction between P. pachyrhizi and Arabidopsis thaliana that the fungus in early infection induces jasmonic acid (JA) pathway to the detriment of the salicylic acid (SA) pathway as a mechanism to the establishment of infection. In this study, we verified that this mechanism might also be occurring during the compatible interaction in soybean (Glycine max L. Merril). It was demonstrated that P. pachyrhizi triggers a JA pathway during the early and late stages of infection in a susceptible soybean cultivar. The expression of the GmbZIP89 was induced in a biphasic profile, similarly to other JA responsive genes, which indicates a new marker gene for this signaling pathway. Additionally, plants silenced for GmbZIP89 (iGmZIP89) by the virus-induced gene silencing (VIGS) approach present lower severity of infection and higher expression of pathogenesis related protein 1 (PR1). The lower disease severity showed that the iGmbZIP89 plants became more resistant to infection. These data corroborate the hypothesis that the GmbZIP89 may be a resistance negative regulator. In conclusion, we demonstrated that P. pachyrhizi mimics a necrotrophic fungus and activates the JA/ET pathway in soybean. It is possible to suppose that its direct penetration on epidermal cells or fungal effectors may modulate the expression of target genes aiming the activation of the JA pathway and inhibition of SA defense.

Genome-wide identification and expression analysis of dormancy-associated gene 1/auxin repressed protein (DRM1/ARP) gene family in Glycine max.

Dormancy-Associated gene 1/Auxin Repressed protein (DRM1/ARP) genes are responsive to hormones involved in defense response to biotic stress, such as salicylic acid (SA) and methyl jasmonate (MeJA), as well as to hormones that regulate plant growth and development, including auxins. These characteristics suggest that this gene family may be an important link between the response to pathogens and plant growth and development. In this investigation, the DRM1/ARP genes were identified in the genome of four legume species. The deduced proteins were separated into three distinct groups, according to their sequence conservation. The expression profile of soybean genes from each group was measured in different organs, after treatment with auxin and MeJA and in response to the nematode Meloidogyne javanica. The results demonstrated that this soybean gene family is predominantly expressed in root. The time auxin takes to alter DRM1/ARP expression suggests that these genes can be classified as a late response to auxin. Nevertheless, only the groups 1 and 3 are induced in roots infected by M. javanica and only group 3 is induced by MeJA, which indicates a high level of complexity in expression control mechanisms of DRM1/ARP family in soybean.

Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.

The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium.

Supplemented powdered coconut water (ACP-406®) promotes growth of goat secondary follicles and oocyte meiotic resumption.

The objective of this study was to test the efficiency of powdered coconut water (ACP-406®) base-medium without or with the addition of supplements on in vitro culture of isolated goat secondary follicles. Follicles were cultured for 18 days in α-MEM or in ACP-406®, both without supplements (referred to as α-MEM and ACP, respectively), or both supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine, and ascorbic acid (referred to as α-MEM+ and ACP+). Follicular morphology, antrum formation, follicular and oocyte diameter, levels of glutathione (GSH), and chromatin configuration after in vitro maturation were evaluated. At the end of culture, ACP-406® base-medium (without or with supplements) showed a higher (P < 0.05) percentage of normal follicles than α-MEM (without or with supplements). Antrum formation was similar among α-MEM+, ACP and ACP+, and significantly higher than α-MEM without supplements. The follicular diameter was greater in ACP+ than α-MEM, and similar to other treatments. Moreover, fully and daily grown rates were higher (P < 0.05) in ACP-406® base-medium (without or with supplements) than α-MEM (without or with supplements). Levels of GSH were similar between ACP+ and α-MEM+ treatments. Both ACP+ and α-MEM+ allowed meiotic resumption without a significant difference between the two groups. In conclusion, supplemented ACP-406® base-medium maintained follicular survival and promoted the development as well as meiotic resumption of isolated goat secondary follicles cultured in vitro for 18 days.

Centralidade do trabalho e saúde mental / Centrality of work for mental health

Partindo da noção de trabalho vivo, Christophe Dejours apresenta a tese da centralidade do trabalho para a subjetividade e a saúde mental. Para tanto, traz à baila a discussão sobre: a atividade, a insuficiência da tarefa e a experiência de fracasso diante do real; bem como a inteligência do corpo e a afetividade, marcando sua transformação à medida que realiza a atividade, em uma relação dinâmica entre o corpo, a subjetividade e a atividade. Não sendo o trabalho algo solipsista, tais elementos se entrecruzam em meio a outrem, o que requisita a cooperação e os aspectos complexos da formulação de regras para a própria atividade e demanda a confiança, a deliberação coletiva. É nesse coletivo, no viver juntos, que pode ocorrer o reconhecimento que, realizado sobre o que é feito, permite a realização de si no campo social, voltando-se para quem o faz, para uma identidade, que é o que protege a saúde mental. Na ausência da convivialidade e reconhecimento, o trabalho produz sofrimento patógeno. Desse modo, Dejours demonstra que nunca há neutralidade do trabalho no que diz respeito à subjetividade e à identidade, possibilitando a realização de si ou sua destruição

Based on the notion of living work, Christophe Dejours presents the thesis of the centrality of work for subjectivity and mental health. To do so, he discusses activity, the insufficiency of the task, the experience of failure in the face of reality, and the intelligence of the body and affectivity, marking its transformation as it performs the activity in a dynamic relationship between the body, subjectivity, and the activity. Rather than configuring a solipsistic text, such elements intersect each other, requiring cooperation and the complex aspects of formulating rules for the activity itself, which requires trust and collective deliberation. It is in this collective, in living together, that recognition can occur, which , based on what is done, enables self-realization in the social field, turning to those who do it and to an identity, which is what protects mental health. In the absence of conviviality and recognition, work produces pathogenic suffering. Thus, the author shows that work is never neutral toward subjectivity and identity, enabling self-realization or its own destruction

Arbuscular mycorrhizal fungi and foliar phosphorus inorganic supply alleviate salt stress effects in physiological attributes, but only arbuscular mycorrhizal fungi increase biomass in woody species of a semiarid environment.

Salinity may limit plant growth especially in arid and semiarid regions. Arbuscular mycorrhizal fungi (AMF) and the supply of inorganic phosphorus (Pi) could alleviate the negative effects of such stress by improvement in stomatal conductance, photosynthesis and biomass. The aim of this study is to evaluate the ecophysiological performance of Cenostigma pyramidale (Tul.) E. Gagnon & G. P. Lewis (Fabaceae) in a greenhouse under salinity conditions in combination with the supply of AMF and leaf Pi. The experiment was conducted in a factorial design considering two levels of salinity (+NaCl and -NaCl), two levels of AMF (+AMF and -AMF) and two levels of leaf Pi supply (+Pi and -Pi). The variables gas exchange, leaf primary metabolism, dry biomass and nutrients were measured. Plants with AMF under non-saline conditions presented a high photosynthesis and biomass. In saline conditions, AMF promoted lower decrease in photosynthesis, high shoot dry matter and low content of leaf and root Na+ and Cl-. Plants treated with leaf Pi increased biomass and photosynthetic pigments under both conditions and accumulated more Cl- in shoots under salinity conditions. When combined, AMF * Pi increased photosynthesis only in non-saline conditions. Plants under salinity conditions without AMF and Pi had higher decreases in gas exchange and high content of Cl- in roots. Therefore, C. pyramidale plants improved their metabolism under both growth conditions in the presence of AMF, Pi or a combination of both. However, the greatest increases in growth and tolerance to salinity occurred in the isolated presence of AMF.

Arbuscular mycorrhizal fungi improve photosynthetic energy use efficiency and decrease foliar construction cost under recurrent water deficit in woody evergreen species.

Plants suffer recurrent cycles of water deficit in semiarid regions and have several mechanisms to tolerate low water availability. Thus, arbuscular mycorrhizal fungi (AMF) can alleviate deleterious effects of stress. In this study, Cynophalla flexuosa plants, a woody evergreen species from semiarid, when associated with AMF were exposed to two consecutive cycles of water deficit. Leaf primary metabolism, specific leaf area (SLA), leaf construction cost (CC) and photosynthetic energy use efficiency (PEUE) were measured. The maximum stress occurred on seven days (cycle 1) and ten days (cycle 2) after suspending irrigation (photosynthesis close to zero). The rehydration was performed for three days after each maximum stress. In both cycles, plants submitted to water deficit showed reduced gas exchange and leaf relative water content. However, Drought + AMF plants had significantly larger leaf relative water content in cycle 2. At cycle 1, the SLA was larger in non-inoculated plants, while CC was higher in inoculated plants. At cycle 2, Drought + AMF treatment had lower CC and large SLA compared to control, and high PEUE compared to Drought plants. These responses suggest AMFs increase tolerance of C. flexuosa to recurrent water deficit, mainly in cycle 2, reducing the CC, promoting the improvement of SLA and PEUE, leading to higher photosynthetic area. Thus, our result emphasizes the importance of studies on recurrence of water deficit, a common condition in semiarid environments.

Sensory processing and engagement: a systematic review / Processamento sensorial e engajamento: uma revisão sistemática

Abstract The ability of children to organize themselves sensorially in a school environment to remain engaged in their routine activities has been minimally explored by occupational therapists. This study aimed to identify research on sensory processing and engagement carried out by occupational therapists over the past decade. Moreover, it seeks to analyze the depth and quality of discussions regarding the relationship between sensory processing and children's engagement in their occupations. A systematic review was conducted utilizing the descriptors: sensory processing, engagement, and occupational therapy. These descriptors were combined through the AND operator in the VHL, PubMed, and Web of Science databases. Included articles, whether in English or other languages, defined and measured sensory processing; highlighted a relationship between child sensory processing and occupational engagement; were published within the past 10 years (2008 to 2018); underwent peer-review; are available in full; involved participants aged 0 to 7 years; and were authored by occupational therapists. The review process was performed by two authors using predefined data fields, which included study quality indicators. The results identified a mere nine articles on this specific theme. An assessment based on the OT Seeker scale revealed scores of moderate magnitude. This suggests a pressing need for occupational therapists to invest in the expansion of discerning research, aiming to elevate scientific evidence regarding the relationship between sensory processing and occupational engagement.

Resumo A capacidade das crianças de se organizarem sensorialmente em ambiente escolar para se manterem engajadas em suas atividades rotineiras tem sido pouco explorada por terapeutas ocupacionais. Este estudo teve como objetivo identificar pesquisas sobre processamento sensorial e engajamento realizadas por terapeutas ocupacionais na última década, bem como avaliar a qualidade das discussões sobre a relação entre processamento sensorial e engajamento de crianças em suas ocupações. Trata-se de uma revisão sistemática realizada a partir da busca dos descritores: processamento sensorial, engajamento e terapia ocupacional, combinados segundo o operador AND, nas plataformas BVS, PubMed e Web of Science. Os artigos incluídos, tanto em inglês e como em outras línguas, definiram e mediram processamento sensorial; apontaram relação entre processamento sensorial e engajamento ocupacional infantil; foram publicados nos últimos 10 anos (de 2008 a 2018); revisados ​​por pares; estão disponíveis na íntegra; envolveram participantes com idade entre 0 e 7 anos, e foram escritos por terapeutas ocupacionais. As revisões foram realizadas por duas autoras usando campos de dados predefinidos, incluindo indicadores de qualidade do estudo. Os resultados identificaram apenas nove artigos sobre esse tema específico. A análise da classificação, com base na escala OT Seeker, revelou escores de magnitude moderada, o que sugere a necessidade de terapeutas ocupacionais investirem na ampliação de pesquisas criteriosas a fim de aumentar os níveis de evidência científica sobre a relação entre processamento sensorial e engajamento ocupacional.

Predominance of rotavirus P[4]G2 in a vaccinated population, Brazil.

We identified 21 rotaviruses in 129 patients with diarrhea in a Brazilian city with high rotavirus vaccine coverage. All rotaviruses were genotype P[4]G2 with 1 mixed infection with P[NT]G9. Although virus predominance could have occurred randomly, the vaccine may be less protective against P[4]G2. Prospective surveillance is urgently needed.

[Anthropometric cranial measurements of normal newborn in Sergipe--Northeast of Brazil]. / Estudo antropométrico do crânio de recém-nascidos normais em Sergipe.

The anthropometric measurement of the skull is essential for the evaluation of the newborn. The anthropometric measurements utilized at the present time are based in the results obtained for more than five decades, which are not able to determine a national pattern mostly likely due to some ethnic influences. We carried out an analytical descriptive study in a maternity hospital in Aracaju-Sergipe, Northeast of Brazil. Measurements of cephalic perimeter, biauricular and anteroposterior distances, cephalic index and fontanels were obtained from 450 newborns with gestational age from 37 and 42 weeks; 49.3% were male and 50.6% female. The cephalic perimeter ranged from 30.0 cm to 39.8 cm with mean value of 34.14+/-2.48 P50 34 cm, and cephalic index ranged from 0.69 and 1.13 with mean value of 0.98+/-0.06 P50 1. A comparison was made between brazilian and foreign studies; the results of the newborn from Sergipe were closer to results obtained in the southeast region than the ones obtained in the northeast region itself. The possibility of ethnical influences was raised as well as the need to design a multicentric study in order to define an anthropometric profile of the Brazilian newborn.