Molecular characterization and RSV Co-infection of Nicotiana benthamiana with three distinct begomoviruses.

Geminiviruses constitute a family of plant viruses with characteristic twinned quasi-icosahedral virions and a small circular DNA genome. Geminiviruses, especially begomoviruses, cause substantial economic losses in tropical and subtropical regions globally. Geminiviruses use the host's transcriptional mechanisms to synthesize their mRNAs. They are considered as an attractive model to understand the transcription mechanism of their host plants. Experiments were conducted to identify transcriptional start sites (TSSs) of the three begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Corchorus yellow vein virus (CoYVV), and Ramie mosaic virus (RamV). We first rub-inoculated Rice stripe tenuivirus (RSV), a segmented negative-sense RNA virus that uses cap-snatching to produce capped viral mRNAs, into N. benthamiana. After the inoculation, RSV-infected N. benthamiana were super-infected by CoYVV, CLCuMuV, or RamV, respectively. The capped-RNA leaders snatched by RSV were obtained by determining the 5'-ends of RSV mRNA with high throughput sequencing. Afterwards, snatched capped-RNA leaders of RSV were mapped onto the genome of each begomovirus and those matching the begomoviral genome were considered to come from the 5' ends of assumed begomoviral mRNAs. In this way, TSSs of begomoviruses were obtained. After mapping these TSSs onto the genome of the respective begomovirus, it was found very commonly that a begomovirus can use many different TSSs to transcribe the same gene, producing many different mRNA isoforms containing the corresponding open reading frames (ORFs).

Effect of cadmium on the regulatory mechanism of steroidogenic pathway of Leydig cells during spermatogenesis.

Cadmium is a male reproductive toxicant that interacts with a variety of pathogenetic mechanisms. However, the effect of cadmium on the regulatory mechanism of the steroidogenic pathway of Leydig cells during spermatogenesis is still ambiguous. Light microscopy, Western blot, immunohistochemistry, immunofluorescence, and quantitative polymerase chain reaction were performed to study the regulatory mechanism of the steroidogenic pathway of Leydig cells during spermatogenesis. The results indicated that in the control group, Leydig cells showed dynamic immunoreactivity and immunosignaling action with a strong positive significant secretion of 3ß-hydroxysteroid hydrogenase (3ß-HSD) in the interstitial compartment of the testis. Leydig cells showed a high active regulator mechanism of the steroidogenic pathway with increased the proteins and genes expression level of steroidogenic acute regulatory protein (STAR), cytochrome P450 cholesterol (CYP11A1), cytochrome P450 cholesterol (CYP17A1), 3ß-hydroxysteroid hydrogenase (3ß-HSD) 17ß-hydroxysteroid hydrogenase (17ß-HSD), and androgen receptor (AR) that maintained the healthy and vigorous progressive motile spermatozoa. However, on treatment with cadmium, Leydig cells were irregularly dispersed in the interstitial compartment of the testis. Leydig cells showed reduced immunoreactivity and immunosignaling of 3ß-HSD protein. Meanwhile, cadmium impaired the regulatory mechanism of the steroidogenic process of the Leydig cells with reduced protein and gene expression levels of STAR, CYP11A1, CYP17A1, 3ß-HSD, 17ß-HSD, and AR in the testis. Additionally, treatment with cadmium impaired the serum LH, FSH, and testosterone levels in blood as compared to control. This study explores the hazardous effect of cadmium on the regulatory mechanism of the steroidogenic pathway of Leydig cells during spermatogenesis.

Potassium and zinc improves physiological performance, nutrient use efficiency, and productivity of wheat.

Despite the critical role of balanced nutrition in crop productivity, the use of potash (K) and zinc (Zn) is not much practiced by Pakistani farmers. The reduced nutrient uptake and crop productivity together increase the costs associated with fertilization and revisit farmers' confidence in the efficacy and profitability of fertilizers. To address this problem, a field study was conducted in the research area of the MNS-University of Agriculture, Multan, in collaboration with Engro Fertilizers Limited. The research plan consisted of five treatments, including T1 = control (without N, P, K, and Zn fertilizers), T2 = NP in practice (NP at 32-23-0 kg acre-1), T3 = recommended NP (NP at 48-34.5 kg acre-1), T4 = balanced NPK (NP+K at 48-34.5-30 kg acre-1), and T5 = balanced NPK + Zn (NPK+Zn at 48-34.5-30 + 7.5 kg acre-1). Wheat was used as a test crop, and its growth, yield, and physiological and nutritional parameters were studied. The results indicated that NPK+Zn balanced nutrition increased plant height, spike length, photosynthetic rate, water use efficiency, transpiration rate, stomatal conductance, and grain yield by 13%, 15%, 44%, 60%, 63%, 39%, and 78%, respectively, compared with the control. It was found that the combined application of NP, K, and Zn improved the recovery efficiency of applied nutrients, i.e., nitrogen recovery efficiency (NRE) by 230%, phosphorus recovery efficiency (PRE) by 136%, potassium recovery efficiency (KRE) by 135%, and zinc recovery efficiency (ZnRE) by 136% compared to NP-alone application. Agronomic use efficiency of applied fertilizers, such as potassium agronomic use efficiency (KAUE) by 71%, phosphorus agronomic use efficiency (PAUE) by 72%, nitrogen agronomic use efficiency (NAUE) by 70%, and zinc agronomic use efficiency (ZnAUE) by 72%, was observed compared to NP-alone application. The results showed that NPUE, PPUE, NPUE, and ZnPUE were reduced by 5%, 3%, 3%, and 5%, respectively, compared to NP-alone application. Our findings suggest that K and Zn should be made an essential part of wheat nutrition management for higher yield and better quality of produce.

Effects of graded levels of dietary protein supplementation on milk yield, body weight gain, blood biochemical parameters, and gut microbiota in lactating ewes.

Diet-associated characteristics such as dietary protein levels can modulate the composition and diversity of the gut microbiota, leading to effects on the productive performance and overall health of animals. The objective of this study was to see how changes in dietary protein levels affect milk yield, body weight gain, blood biochemical parameters, and gut microbiota in lactating ewes. In a completely randomized design, eighteen ewes were randomly assigned to three groups (n = 6 ewes/group), and each group was assigned to one of three dietary treatments with different protein contents. The ewes' groups were fed on 8.38% (S-I), 10.42% (S-m), and 13.93% (S-h) dietary protein levels on a dry basis. The body weight gain and milk yield were greater (p < 0.05) in ewes fed the S-h dietary treatment than in those fed the S-m and S-1 diets, respectively. However, milk protein contents were similar (p > 0.05) across the treatments. The blood glucose, total protein, cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein, lactate, creatinine, and C-reactive protein contents of lactating ewes were not influenced (p > 0.05) by different dietary protein levels. The alanine transaminase, aminotransferase, and lactate dehydrogenase activities were also not changed (p > 0.05) across the groups. However, blood urea nitrogen and albumin contents of lactating ewes were changed (p < 0.05) with increasing levels of dietary protein, and these metabolite concentrations were higher (p < 0.05) for S-h than the rest of the treatments. In the different treatment groups, Firmicutes and Bacteroidetes were found to be the most dominant phyla. However, the abundance of Lachnospiraceae species decreased as dietary protein levels increased. Within the Bacteroidetes phylum, Rikenellaceae were more abundant, followed by Prevotellaceae, in ewes fed the S-m diet compared to those fed the other diets. Based on the results, feeding at an optimal protein level improved milk yield and body weight gain through modifying the digestive tract's beneficial bacterial communities. The results of blood metabolites suggested that feeding higher-protein diets has no negative impact on health.

Fate of 15N-labelled urea as affected by long-term manure substitution.

Quantifying the fate of fertilizer nitrogen (N) is essential to develop more sustainable agricultural fertilization practices. However, the fate of chemical fertilizer N, particularly in long-term manure substitution treatment regimes, is not fully understood. The present study aimed to investigate the fate of 15N-labelled urea in a chemical fertilizer treatment (CF, 240 kg 15N ha-1) and N manure 50 % substitution treatment (1/2N + M, 120 kg 15N ha-1 + 120 kg manure N ha-1) in two continuous crop seasons, based on a 10-year long-term experiment in the North China Plain (NCP). The results showed that manure substitution greatly enhanced 15N use efficiency (15NUE) (39.9 % vs. 31.3 %) and suppressed 15N loss (6.9 % vs. 7.5 %) compared with the CF treatment in the first crop. However, the N2O emissions factor in the 1/2N + M treatment was increased by 0.1 % (0.5 kg 15N ha-1 for CF vs. 0.4 kg 15N ha-1 for 1/2N + M) compared with the CF treatment, although N leaching and NH3 volatilization rates decreased by 0.2 % (10.8 kg 15N ha-1 for CF vs. 5.1 kg 15N ha-1 for 1/2N + M) and 0.5 % (6.6 kg 15N ha-1 for CF vs. 2.8 kg 15N ha-1 for 1/2N + M), respectively. In which, only NH3 volatilization presented significantly difference between treatments. It is important to note that in the second crop, the residual 15N in soil (0-20 cm) remained mostly in the soil for the CF (79.1 %) and the 1/2N + M treatment (85.3 %), and contributed less to crop N uptake (3.3 % vs. 0.8 %) and leached losses (2.2 % vs. 0.6 %). This proved that manure substitution could enhance the stabilization of chemical N. These results suggested that long-term manure substitution effectively increases NUE, suppresses N loss, and improves N stabilization in soil, but negative impacts such as N2O emissions due to climate change should be investigated further.

Transgenerational effect of Afidopyropen on Bemisia tabaci Gennadius (Homoptera: Aleyrodidae).

Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) is the most devastating insect-pest in cotton crop. It is vector of the cotton leaf curl virus (CLCV) and is responsible for huge losses to cotton industry in Pakistan and worldwide. It is mainly controlled by insecticides but the injudicious use of insecticides has resulted in insecticide resistance and population resurgence in addition to various harmful effects on the humans, non-target organisms and the environment. Transgenerational studies are very helpful to choose a best insecticidal option. In the current study, age-stage two-sex life table analysis was used to identify transgenerational effects of sublethal doses of afidopyropen. The adults of B. tabaci were treated with three concentrations of afidopyropen i.e., LC10, LC30 and LC50. The results indicated significant changes in the progeny i.e. the fecundity decreased in treated population; and female and male longevity of their progeny were more in control as compared to treated populations. Similarly, population parameters like intrinsic rate of growth (r), net reproductive rate (R0) and limiting rate of growth (λ) were significantly decreased in the treated adult progeny with values of 0.08-0.11, 4.85-7.46 and 1.09-1.12 per day, respectively. Based on the reduced biotic potential, afidopyropen can be suggested as an effective alternative option for the management of B. tabaci.

Role of sugarcane industrial byproducts on soil physicochemical properties and metal accumulation in rice.

Sugar industry produces a variety of organic byproducts causing disposal as well as environmental issues. This study investigated the safe use of these byproducts in assessment of soil physicochemical properties and metal accumulation in rice. A field experiment was performed with following treatments: control only NPK (CF), NPK + sugarcane bagasse (SB), NPK + press mud (PM), NPK + sugarcane vinasse (SV), NPK + SB + PM (SB + PM), NPK + SB + SV (SB + SV), NPK + PM + SV (PM + SV), NPK + SB + PM + SV (SB + PM + SV). Total byproduct input was (1.25 t ha-1) as recommended for organic inputs in the local area. The results indicated that integrated use of these amendments with chemical fertilization improved soil properties and rice yield. Organic matter was significantly improved in SB + SV (191.3%), SB + PM + SV (164.4%), and SB + PM (150.9%). Total N was significantly enhanced in SB + SV (193%), SB + PM + SV (166%), and SB + PM (152.5%); extractable P was high in SB (103%), PM + SV (89.7%), and SB + PM (51%); extractable K was significantly improved in PM (39.6%) and SB (33.4%); extractable Zn was significantly enhanced in SB (1172.8%), SV (829.2%) and PM (819.1%) in soil. Rice grain yield was significantly enhanced in SB (213.1%) and PM (208.8%) while combined application also improved the yield with reference to the CF. The application of SB + PM improved N (58.7%), P (27.4%), K (11.5%), and Zn (166.4%) concentration in grain, while metal accumulation was within the permissible limit. Zn concentration was significantly enhanced in SB + PM (166.4%) whereas the concentration of Cd and Pb was significantly reduced with the application of byproducts. Health assessment results showed no harmful effects for humans. Results conclude that these byproducts are good nutrients source and improve soil physicochemical properties without any health hazards.

Biostimulants induce positive changes in the radish morpho-physiology and yield.

An ever-increasing population has issued an open challenge to the agricultural sector to provide enough food in a sustainable manner. The upsurge in chemical fertilizers to enhance food production had resulted in environmental problems. The objective of the current study is to assess the utilization of biostimulants for sustainable agricultural production as an alternative to chemical fertilization. For this purpose, two pot experiments were conducted to examine the response of radish against individual and combined applications of biostimulants. In the first experiment, the effects of chemical fertilizer (CK), glycine (G), lysine (L), aspartic acid (A), and vitamin B complex (V) were studied. The results demonstrated that V significantly improved the transpiration rate (81.79%), stomatal conductance (179.17%), fresh weight (478.31%), and moisture content (2.50%). In the second experiment, tested treatments included chemical fertilizer (CK), Isabion® (I), glycine + lysine + aspartic acid (GLA), moringa leaf extract + GLA (M1), 25% NPK + M1 (M2). The doses of biostimulants were 5g L-1 glycine, 1g L-1 lysine, 2g L-1 aspartic acid, and 10 ml L-1 moringa leaf extract. The photosynthetic rate improved significantly with GLA (327.01%), M1 (219.60%), and M2 (22.16%), while the transpiration rate was enhanced with GLA (53.14%) and M2 (17.86%) compared to the Ck. In addition, M1 increased the stomatal conductance (54.84%), internal CO2 concentration (0.83%), plant fresh weight (201.81%), and dry weight (101.46%) as compared to CK. This study concludes that biostimulants can effectively contribute to the sustainable cultivation of radish with better growth and yield.

A Novel Protein Elicitor (PELL1) Extracted from Lecanicillium lecanii Induced Resistance against Bemisia tabaci (Hemiptera: Aleyrodidae) in Gossypium hirsutum L.

Protein elicitors play a key role in signaling or displaying plant defense mechanism and emerging as vital tools for biocontrol of insects. This study was aimed at the characterization of the novel protein elicitor isolated from entomopathogenic fungi Lecanicillium lecanii (V3) strain and its activity against whitefly, Bemisia tabaci, in cotton (Gossypium hirsutum L.). The sequence of purified elicitor protein showed 100% similarity with hypothetical protein LEL_00878 (Cordyceps confragosa RCEF 1005) (GenBank accession no. OAA81333.1). This novel protein elicitor has 253 amino acid residues and 762 bp with a molecular mass of 29 kDa. Their combatant protein was expressed in Escherichia coli using pET-28a (+) plasmid. Bioassay was revealed to quantify the impact of numerous concentrations of protein (i.e., 58.32, 41.22, and 35.41 µg/ml) on the fecundity rate of B tabaci on cotton plants. Bioassay results exhibited a significant effect (P ≤ 0.001) of all the concentrations of protein on the fecundity rate of B. tabaci. In addition, the gene expression analysis found a significant upregulation of the major genes associated with salicylic acid (SA) and jasmonic acid (JA) defense pathways in elicitor protein-treated plants. Our results showed that the potential application of novel protein elicitor derived from Lecanicillium lecanii will be used as future biointensive controlling approaches against whitefly, Bemisia tabaci.

Correction: Path analysis based on genetic association of yield components and insects pest in upland cotton varieties.

[This corrects the article DOI: 10.1371/journal.pone.0260971.].

The Impact of Seeding Density and Nitrogen Rates on Forage Yield and Quality of Avena sativa L.

Green forage is an excellent feed source for livestock. It is an integral part of livestock production to accomplish the demands for butter, milk, and other derivatives for human utilization. Livestock contributes 11.39% towards the gross domestic product of Pakistan and 58.33% in agricultural farming. Livestock face shortage or insufficient supply of green fodder during the winter season, which ultimately reduces milk yield. Oat (Avena sativa L.) is a major forage crop in the winter season; however, several biotic and abiotic factors negatively affect its yields. Low soil fertility, particularly nitrogen deficiency, is regarded as one of the few reasons responsible for the low forage yield of oat. Low organic matter content in the soil, suboptimal agronomic practices, and harsh climatic conditions are the other major reasons for low oat yield. Seed rate and different nitrogen rates significantly alter green forage yield and quality of oat. This study assessed the impact of different seeding densities and nitrogen (N) doses on the forage yield of oat. Three seeding densities (70, 80, and 90 kg ha-1) and five N doses (0, 40, 80, 120, and 160 kg ha-1) were included in the study. The interactive effect of seeding density and N doses significantly altered green forage yield and quality attributes of oat. The highest green forage yield (54.67 t ha-1) was noted for the interaction among 90 kg seed rate ha-1 and 160 kg N ha-1. Similarly, the highest germination count (140 m-2), number of tillers (5.97 m-2), plant height (122.97 cm), number of leaves per plant (24.50 m-2), leaf area per tiller (123.18 cm2), fresh weight (5.47 kg m-2), dry weight (1692 g m-2), dry matter yield (20.90 t ha-1), crude protein (10.54%), crude fiber (31.62%), and total ash (9.39%) were recorded for the interactive effect of 90 kg seed rate ha-1 and 160 kg N ha-1. Economic analysis revealed that interaction between 90 kg seed rate ha-1 with 120 and 160 kg N ha-1 was superior to others with higher benefit: cost ratio and net economic returns. It is recommended that the oat seed rate of forage oat crop must be kept at 90 kg ha-1 and it should be supplied 120 kg N ha-1 for higher yield, better quality, and more economic returns.

Toxicological Effects of Sewage Water on Chick Embryonic Development.

For toxicity research, a total of 100 fertilized nonincubated eggs were used for this study. There were two trials in this experiment which were further divided into 2 phases based on a different days of sewage water treatment and observation days. In each trial, 50 eggs were used and divided into 5 groups. Group A, B, and C were treated with three different concentrations of pure and diluted sewage water (100%, 70%, and 30%), respectively. Control group D was given 0.3 ml saline solution (0.9% NaCl) and group E was uninjected. Different parameters such as the embryo's body weight, body length, forelimb length, hindlimb length, and head diameter were determined. In trial 1, eggs were treated with sewage water on 7th day of incubation and opened on 8th day (phase I) and 9th day (phase II). When the trial 1 (phase I) findings were compared to the control groups, it was observed that body weight, body length, forelimb length, and hindlimb length were highly statistically significant differences (p < 0.01), but the head diameter was not significant (p > 0.05). Phase II result showed embryo's head diameter was a highly statistically significant difference (p < 0.01), whereas forelimb length was significant (p < 0.05), and body weight, body length, and hindlimb length were nonsignificant (p > 0.05). In trial 2, eggs were treated with sewage water on 14th day of incubation and opened on 15th day (phase I) and 16th day (phase II). Results of 15th day showed a highly statistically significant (p < 0.01) difference in hindlimb length, while body weight, body length, forelimb length, and head diameter were nonsignificant (p > 0.05). Phase II of trial 2 showed that on 16th day, body weight, body length, forelimb length, hindlimb length, and head diameter showed a nonsignificant (p > 0.05) difference between experimental and control groups. Embryos were observed to be deforming on the 9th day (after 48 hours of exposure to sewage water). Other phases showed no signs of deformation. Except on 8th day of incubation, dose-related mortalities were present in experimental groups, while the control group showed no mortality.

Effect of Vegetable Waste on Growth Performance and Hematology of Broiler Chicks.

Vegetable waste (spinach, potato, and cauliflower) is a rich and natural source of nutrients, potentially good for supplying minerals, essential amino acids, and antioxidants to the birds. Relatively, its cost very low, easily to accessible, easily process & pose little risk of illness. The aim of present study was to evaluate the effect of vegetable waste (VW) as feed supplement on growth performance and hematology of broiler chicks. For this purpose, a total of 200 (4 days old) vaccinated chicks were acquired from a commercial hatchery Multan which was acclimated for three weeks (21 days) on basal starter feed after that 25-day-old chicks with uniform body weight were allocated according to a CRD (completely randomized design) into four dietary treatments with three replicates of each contained 15 chicks in 12 pens. In dietary treatments, chicks were feed with basal feed (BF) and supplemented feed with vegetable waste (VW) of spinach, potato, and cauliflower. For this purpose, the dietary treatments included control treatment (T 1) (100% BF+0% VW) and other dietary treatments (T 2) (75% BF+25% VW), (T 3) (50% BF+50% VW), and (T 4) (25% BF+75% VW). The body weight, feed intake, food conversion ratio (FCR), and mortality were checked on weekly and daily basis. For hematology analysis, after the 1st experimental week (25-day-old chicks) and the last 5th experimental week (56-day-old chicks), the samples of blood were gathered from the wing's veins of two birds from each treatment in random way. At the end of five weeks (35 days), birds with uniform average body weight were selected per treatment with three replicates (2 bird/replicate) and then were manually slaughtered according to the Halal method to analyze the weight of internal body organs of broilers by physical and statistical analysis (ANOVA). There was no significant effect (P > 0.05) on feed intake and FCR among all the dietary treatments. But in average, body weight and BWG were higher in treatment (T 2) (P < 0.01) than all other dietary treatments (T 3) and (T 4) and control treatment (T 1). The blood constituents in this study showed that broilers in control treatment (T 1) and other dietary treatments (T 2), (T 3), and (T 4) fed on different doses were significantly (P < 0.01) different from each other. The week 5 (W 5) shows higher values of blood constituents (P < 0.01) than week 1 (W 1). The carcass yield of the chicks fed on different doses showed that they were significantly different (P < 0.01) among the dietary treatments. The VW inclusion 0%, 25%, 50%, and 75% had positive effect on blood constituents and carcass yield of the broiler chicks; they were significantly (P < 0.01) different among the treatments.

Stink bug Agonoscelis spp. (Heteroptera: Pentatomidae) - An emerging threat for seed production in alfalfa crop (Medicago sativa L.) and their successful management.

Fodder crops play an important role in sustainable agriculture as they provide feed for animals, which is ultimately converted to human food. Alfalfa is one of the most important fodder crops having high nutritive value for livestock. However, seed production of alfalfa crop is seriously affected by several factors and the highest reduction in seed yield is caused by stink bug infestation. The current study evaluated different insecticides to control stink bugs during 2016-17. The efficacy of ten insecticides, i.e., acephate, dimethoate, malathion, chlorpyriphos, bifenthrin, lambdacyhalothrin, deltamethrin, acetamiprid, imidacloprid and carbosulfan was tested on Agonoscelis spp. (Heteroptera Pentatomidae). The mortality of stink bug was recorded at one, three, five, seven, ten and fifteen days after insecticide application. Similarly, the population of pollinators was recorded before and one, three and five days after the application of insecticides. It was observed that acetamiprid (81.14%) and acephate (80.65%) caused the highest mortality of stink bug and proved most effective. Insecticides application decreased the pollinators' population one day after spray; however, it was rehabilitated three days after insecticide application. Insecticide application increased seed yield from 28.05 kg/acre (during last four year without chemical control) to 116 kg/acre in 2016-17 (with chemical control). It is concluded that acetamiprid and acephate can be successfully used in integrated management program of increasing alfalfa seed production.

Farmers' knowledge, perception and management of mango mealy bug, Drosicha mangiferae Green (Hemiptera: Monophlebidae), on Mangifera indica in Punjab, Pakistan.

In 2004-05, during the December to February about 141 mango farmers were interviewed during peak activity of mango mealy bug in southern Punjab, Pakistan. The objective was to know the farmers' knowledge, perceptions and practices in the management of mango mealy bug. Most of the farmers (94.33%) reported that Chaunsa variety (king of all mango varieties) was susceptible to mango and irrigation water was the major source of flare up of this pest. Basudin and Supracide were the most commonly used insecticides as 72.92 and 51.77 percent farmers gave positive response and grease bands were applied for the control of mango mealy bug by the majority of the respondents. Hundred percent yield losses was told by 22.7 percent respondents whereas 75 percent, 50 percent and 25 percent losses were reported by 39.7, 31.9 and 14.2 insecticidal spray did not show satisfaction to the respondents for the control of fertilized females of mango mealy bug coming down from the trees. Lack of knowledge about the pest, lack of money, adulterated and shortage of pesticides, lack of unity amongst farmers Further the growers' views were tested in the field for confirmation and small land holdings were the main constraints for the control of mango mealy bug.

Association of blood groups with hepatitis C viremia.

Hepatitis C virus remained a public health problem with approximately half of the patients untreated and undiagnosed. Chronic HCV is a leading cause of cirrhosis, fibrosis, hepatocellular carcinoma and other hepatic morbidities. Active HCV has a prevalence rate of about 1% (71 million). By July, 2019, 10 million population of Pakistan was declared to have active HCV infection. According to World Health Organization, 23,720 people died of hepatitis-related complexities in Pakistan in 2016. Individuals with certain types of ABO blood groups were more susceptible to diverse kinds of infections. For instance, blood types A and AB predisposed individuals to severe malaria, while type O conferred resistance to the many of the protozoan agent. This study was designed to explore the association of hepatitis C viremia to blood groups, Rh factors, age and gender distribution among Pakistani population. Total 246 participants were screened for HCV in Taqwa diagnostics laboratory, Multan and 200 were found positive. They were divided into 4 groups on the basis of their age. First group included patients ranging from 17 to 25 (52), second, third and fourth group included patients from 26 to 34 (92), 35 to 43 (42) and 44 to above (14) respectively. Confirmed Hepatitis C patients were subjected to analysis of blood group, Rh factor and viral load. Results demonstrated that patients having 'O' blood group (60.37%) were reported for high viral load than any of the other blood groups in the patients of Southern Punjab, Pakistan. Furthermore, Rh-negative factor (26.42) was associated with high viral load than that of the Rh-positive factor (73.58). Disclosure practiced that age group (26-34) was reported for the high viral load than that of the any other group of this study. Females were more aggressively affected by HCV Viremia than male because the mean viral load among the females was higher than that of the males. Greater social awareness and gender-sensitive healthcare is necessary to improve the experiences of patients with HCV.

Correction: The impact of Fosetyl-Aluminium application timing on Karnal bunt suppression and economic returns of bread wheat (Triticum aestivum L.).

[This corrects the article DOI: 10.1371/journal.pone.0244931.].

Application of K and Zn Influences the Mineral Accumulation More in Hybrid Than Inbred Maize Cultivars.

Maize (Zea mays L.) is an important crop used for feeding humans and cattle globally. Deficiency of potassium (K) and zinc (Zn) adversely impacts the maize crop productivity and quality. However, the application of these nutrients shows variant responses in different maize cultivars. To understand this perspective, the current study aimed at investigating K and Zn's optimal concentration in different hybrid and inbred maize cultivars. The treatments were based on three zinc levels (0, 6, and 12 mg Zn kg-1) and K levels (0, 30, and 60 mg kg-1), and their respective combinations. The experiment results showed that combined fertilization approaches of Zn and K (Zn12K60) improved the plant biometric, and physiological attributes of maize crop. The results revealed a significant increase in plant height (45%), fresh weight (70%), and dry weight (45%). Similarly, physiological attributes significantly improved the relative water content (76.4%), membrane stability index (77.9%), chlorophyll contents (170%), and photosynthetic rate (130%) in both inbred and hybrid genotypes. Furthermore, Zn and K (Zn12K60) increased transpiration rate (E), stomatal conductance (Ci), and internal CO2. In conclusion, maize hybrids (Neelam and DK-6142) were observed best compared with inbred (Afghoi and P-1543) cultivars with the combined application of Zn and K (Zn12K60). Thus, these inbred varieties should be preferred for fodder requirement with optimum fertilizer (Zn12K60) application in Zn deficient soils.

The impact of Fosetyl-Aluminium application timing on Karnal bunt suppression and economic returns of bread wheat (Triticum aestivum L.).

Fungal pathogens exert severe qualitative and quantitative damages to wheat crop. Karnal bunt of wheat caused by Tilletia indica Mitra, Mundkur is a severe threat to global food security. Nonetheless, T. indica is regulated as a quarantine pest in numerous countries, which further aggravates the situation. Tolerant varieties and appropriate management practices for Karnal bunt are imperative to meet the global wheat demands. This two-year study explored the impact of fungicide [Fosetyl-Aluminium (Aliette)] application timing on allometric traits, disease suppression and economic returns of bread wheat. Four bread wheat cultivars differing in their tolerance to Karnal bunt were used in the study. Fungicide was applied as either seed treatment (ST), foliar application at heading (FAH) or ST + FAH, whereas no application (NA) was taken as control. Lasani-08 performed better than the rest of the cultivars in terms of allometric traits (plant height, leaf area, crop growth rate, photosynthesis, and chlorophyll content), yield and economic returns. Nonetheless, minimal disease severity was recorded for Lasani-08 compared to other cultivars during both years. The ST improved allometric traits of all cultivars; however, ST + FAH resulted in higher yield and economic returns. Cultivar Pasban-90 observed the highest disease severity and performed poor for allometric traits, yield and economic returns. It is concluded that ST + FAH of Fosetyl-Aluminium could be a pragmatic option to cope Karnal bunt of wheat. Nonetheless, Pasban-90 must not be used for cultivation to avoid yield and quality losses.

Effect of host plant on cornucopia of mango fruit flies (Diptera: Tephritidae) and their triumphant management in context of climate change.

A study was performed to assess the preference of fourteen mango cultivars for fruit flies and their management by bagging. So the choice of Tephritid flies to mango cultivars during fruiting phase is crucial. Fourteen different cultivars of mango viz., 'Dusehri', 'Malda', 'Langra' early cultivars, 'Chaunsa', 'Fajri Klan', 'Sensation' medium whereas 'Sanglakhi', 'Retaul-12', 'Mehmood Khan', 'Tukhmi', 'Kala Chaunsa', 'Chitta Chaunsa', 'Dai Wala' and 'Sobey De Ting' late cultivars were assessed for their suitability for fruit flies. The results indicate that the population density of fruit flies was higher on late cultivars like 'Sanglakhi' (20.61 percent), 'Mehmood Khan' (20.22 percent) and 'Reutal-12' (19.92 percent) were proved to be highly susceptible to fruit flies. Among these the cultivar 'Reutal-12' was selected being commercial and future cultivar for the management of fruit flies through bagging. The results reported that the attack of tephritid fruit flies and other insect pests were zero in bagged fruits as compared with control. It was further recorded that the bagged fruits has maximum average fruit weight i.e. 203.50 and 197.83 g per fruit was noted in those treatments where butter paper bag and brown paper bag was wrapped with better coloration as compared with un-bagged fruit with 159.5 g per fruit. Similarly, on an average fruit length were more i.e. 90.17, 91.33 mm in bagged fruit and 85.33 in un-bagged fruits. Furthermore, bagged fruits have zero incidence of disease with reduced fruit crack, fruit sunburn, mechanical damage, bird damage, fruit blemished and agrochemical residues on the fruit. So, it is concluded that the special attention should be given on 'Reutal-12' for the management of fruit flies when devising an IPM program for the control of fruit flies. Further, bagging has proved to be the good agricultural practices for the production of quality mango.