Super-resolution microscopy for the study of store-operated calcium entry.

The use of a variety of techniques based on super-resolution (SR) microscopy unveiled a close and complex relationship between cytoskeleton reorganization and SOCE. By using SR microscopy many new proteins involved in SOCE regulation have been identified over the last few years. Many enigmas remain unsolved in this highly dynamic field, however, recent developments in SR microscopy promise new answers soon. In the present review, we describe the most relevant findings in SOCE components and SOCE modulation using different methods derived from SR microscopy.

Colecistectomia aberta com preservação do músculo reto abdominal: pós-operatório menos doloroso? / Preservation of open cholecystectomy with abdominal rectus muscle: less postoperative pain

Objetivo: Observar se a técnica de colecistectomia aberta com preservação do músculo reto abdominal proporcionaaos pacientes um pós-operatório menos doloroso. Métodos: Estudo prospectivo, exploratório e documental de 50 pacientes com diagnóstico clínico e imaginológico compatíveis com colecistopatia crônica calculosa,submetidos à colecistectomia aberta com preservação do músculo reto abdominal, no Hospital São José,em Criciúma/SC, no período de janeiro a julho de 2007. Foram excluídos do estudo pacientes com idade inferiora 13 anos, pacientes com colecistopatia aguda, pacientes com complicações em árvore biliar e pacientes com quadro de peritonite generalizada. Métodos: Quanto ao perfil da amostra, a maioria dos pacientes era do sexo feminino (82%), sendo a média de idade do total de pacientes 50,22 anos. Dentre aequipe de médicos e cirurgiões residentes que participaram das cirurgias, 84% negaram dificuldades técnicas. O número de perfurações de vesícula biliar durante o trans-operatório ocorreu em 20 pacientes (40%). O tempo de internação foi de 2 dias para 49 pacientes e 3 dias para 01 paciente. Quanto à dor no pós-operatório, 68% dos pacientes afirmaram não estar sentindo dor no primeiro dia do pós-operatório, 22% relataram dor branda, 8% dor moderada e 2% dor severa. Conclusões: De acordo com este estudo a dor sentida no pós-operatório foi relatada como mínima ou ausente pela maioria dos pacientes.

Objective: To observe if open cholecystectomy with rectus abdominal muscle preservation propitiate for patients a less pain in postoperative.Methods: Prospective, exploratory and documental study of 50 patients with clinical and image diagnosis compatible with chronic calculi cholecystitis treated with open cholecystectomy with rectus muscle preservation, in São José Hospital, Criciúma/SC, from January until July of 2007. It was excluded patients under of 13 years old, patients with acute cholecystitis, patients with complications in bile duct and patients with generalized peritonitis. Results: About sample profile, most patients werefemale sex (82%) and the average age of total patients was 50,22 years. In the staff of surgeons doctors and resident surgeons doctors that participate of surgeries, 84% declared nonexistent technique difficulties. The number of gallbladder perforations during the surgery occurred in 20 patients (40%). The time of internment was 2 days for 49 patients and 3 for 01 patient. Aboutthe pain result in postoperative, 68% of patients said that they were not felling pain in first day of postoperative,22% report light pain, 8% moderate pain and 2% severe pain. Conclusions: According to this study the pain felton postoperative was minimum or absent for the most patients.