RESUMEN
While plants produce reactive oxygen species (ROS) for stress signaling and pathogen defense, they need to remove excessive ROS induced during stress responses in order to minimize oxidative damage. How can plants fine-tune this balance and meet such conflicting needs? Here, we show that XANTHINE DEHYDROGENASE1 (XDH1) in Arabidopsis thaliana appears to play spatially opposite roles to serve this purpose. Through a large-scale genetic screen, we identified three missense mutations in XDH1 that impair XDH1's enzymatic functions and consequently affect the powdery mildew resistance mediated by RESISTANCE TO POWDERY MILDEW8 (RPW8) in epidermal cells and formation of xanthine-enriched autofluorescent objects in mesophyll cells. Further analyses revealed that in leaf epidermal cells, XDH1 likely functions as an oxidase, along with the NADPH oxidases RbohD and RbohF, to generate superoxide, which is dismutated into H2O2 The resulting enrichment of H2O2 in the fungal haustorial complex within infected epidermal cells helps to constrain the haustorium, thereby contributing to RPW8-dependent and RPW8-independent powdery mildew resistance. By contrast, in leaf mesophyll cells, XDH1 carries out xanthine dehydrogenase activity to produce uric acid in local and systemic tissues to scavenge H2O2 from stressed chloroplasts, thereby protecting plants from stress-induced oxidative damage. Thus, XDH1 plays spatially specified dual and opposing roles in modulation of ROS metabolism during defense responses in Arabidopsis.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimología , Arabidopsis/metabolismo , Xantina Deshidrogenasa/metabolismo , Arabidopsis/genética , Arabidopsis/microbiología , Proteínas de Arabidopsis/genética , Ascomicetos/patogenicidad , Resistencia a la Enfermedad/genética , Resistencia a la Enfermedad/fisiología , Interacciones Huésped-Patógeno/genética , Interacciones Huésped-Patógeno/fisiología , Enfermedades de las Plantas/microbiología , Especies Reactivas de Oxígeno/metabolismo , Xantina Deshidrogenasa/genéticaRESUMEN
Upon penetration of the host cell wall, the powdery mildew fungus develops a feeding structure named the haustorium in the invaded host cell. Concomitant with haustorial biogenesis, the extrahaustorial membrane (EHM) is formed to separate the haustorium from the host cell cytoplasm. The Arabidopsis resistance protein RPW8.2 is specifically targeted to the EHM where it activates haustorium-targeted resistance against powdery mildew. RPW8.2 belongs to a small family with six members in Arabidopsis (Arabidopsis thaliana). Whether Homologs of RPW8 (HR) 1 to HR4 are also localized to the EHM and contribute to resistance has not been determined. Here, we report that overexpression of HR1, HR2, or HR3 led to enhanced resistance to powdery mildew, while genetic depletion of HR2 or HR3 resulted in enhanced susceptibility, indicating that these RPW8 homologs contribute to basal resistance. Interestingly, we found that N-terminally YFP-tagged HR1 to HR3 are also EHM-localized. This suggests that EHM-targeting is an ancestral feature of the RPW8 family. Indeed, two RPW8 homologs from Brassica oleracea tested also exhibit EHM-localization. Domain swapping analysis between HR3 and RPW8.2 suggests that sequence diversification in the N-terminal 146 amino acids of RPW8.2 probably functionally distinguishes it from other family members. Moreover, we found that N-terminally YFP-tagged HR3 is also localized to the plasma membrane and the fungal penetration site (the papilla) in addition to the EHM. Using this unique feature of YFP-HR3, we obtained preliminary evidence to suggest that the EHM is unlikely derived from invagination of the plasma membrane, rather it may be mainly synthesized de novo.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Arabidopsis/microbiología , Membrana Celular/metabolismo , Proteínas de la Membrana/metabolismo , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Ascomicetos/patogenicidad , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Brassica/química , Resistencia a la Enfermedad , Interacciones Huésped-Patógeno , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Proteínas de la Membrana/genética , Enfermedades de las Plantas/microbiología , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente , Transporte de ProteínasRESUMEN
Plants use a tightly regulated immune system to fight off various pathogens. Phospholipase D (PLD) and its product, phosphatidic acid, have been shown to influence plant immunity; however, the underlying mechanisms remain unclear. Here, we show that the Arabidopsis mutants pldα1 and pldδ, respectively, exhibited enhanced resistance and enhanced susceptibility to both well-adapted and poorly adapted powdery mildew pathogens, and a virulent oomycete pathogen, indicating that PLDα1 negatively while PLDδ positively modulates post-penetration resistance. The pldα1δ double mutant showed a similar infection phenotype to pldα1, genetically placing PLDα1 downstream of PLDδ. Detailed genetic analyses of pldδ with mutations in genes for salicylic acid (SA) synthesis (SID2) and/or signaling (EDS1 and PAD4), measurement of SA and jasmonic acid (JA) levels, and expression of their respective reporter genes indicate that PLDδ contributes to basal resistance independent of EDS1/PAD4, SA, and JAsignaling. Interestingly, while PLDα1-enhanced green fluorescent protein (eGFP) was mainly found in the tonoplast before and after haustorium invasion, PLDδ-eGFP's focal accumulation to the plasma membrane around the fungal penetration site appeared to be suppressed by adapted powdery mildew. Together, our results demonstrate that PLDα1 and PLDδ oppositely modulate basal, post-penetration resistance against powdery mildew through a non-canonical mechanism that is independent of EDS1/PAD4, SA, and JA.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimología , Ascomicetos/fisiología , Fosfolipasa D/metabolismo , Enfermedades de las Plantas/inmunología , Ácido Salicílico/metabolismo , Arabidopsis/genética , Arabidopsis/inmunología , Arabidopsis/microbiología , Proteínas de Arabidopsis/genética , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Regulación de la Expresión Génica de las Plantas , Fosfolipasa D/economía , Fosfolipasa D/genética , Enfermedades de las Plantas/microbiología , Inmunidad de la PlantaRESUMEN
The Arabidopsis thaliana resistance to powdery mildew8.2 (RPW8.2) protein is specifically targeted to the extrahaustorial membrane (EHM) encasing the haustorium, or fungal feeding structure, where RPW8.2 activates broad-spectrum resistance against powdery mildew pathogens. How RPW8.2 activates defenses at a precise subcellular locale is not known. Here, we report a comprehensive mutational analysis in which more than 100 RPW8.2 mutants were functionally evaluated for their defense and trafficking properties. We show that three amino acid residues (i.e., threonine-64, valine-68, and aspartic acid-116) are critical for RPW8.2-mediated cell death and resistance to powdery mildew (Golovinomyces cichoracearum UCSC1). Also, we reveal that two arginine (R)- or lysine (K)-enriched short motifs (i.e., R/K-R/K-x-R/K) make up the likely core EHM-targeting signals, which, together with the N-terminal transmembrane domain, define a minimal sequence of 60 amino acids that is necessary and sufficient for EHM localization. In addition, some RPW8.2 mutants localize to the nucleus and/or to a potentially novel membrane that wraps around plastids or plastid-derived stromules. Results from this study not only reveal critical amino acid elements in RPW8.2 that enable haustorium-targeted trafficking and defense, but also provide evidence for the existence of a specific, EHM-oriented membrane trafficking pathway in leaf epidermal cells invaded by powdery mildew.
Asunto(s)
Aminoácidos/inmunología , Proteínas de Arabidopsis/inmunología , Arabidopsis/inmunología , Inmunidad de la Planta , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Arabidopsis/genética , Arabidopsis/microbiología , Proteínas de Arabidopsis/genética , Ascomicetos , Análisis Mutacional de ADN , ADN de Plantas/genética , Resistencia a la Enfermedad , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Enfermedades de las Plantas/inmunología , Enfermedades de las Plantas/microbiología , Hojas de la Planta/citología , Hojas de la Planta/microbiología , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/inmunología , Plantas Modificadas Genéticamente/microbiologíaRESUMEN
Powdery mildew fungal pathogens penetrate the plant cell wall and develop a feeding structure called the haustorium to steal photosynthetate from the host cell. Here, we report that the broad-spectrum mildew resistance protein RPW8.2 from Arabidopsis thaliana is induced and specifically targeted to the extrahaustorial membrane (EHM), an enigmatic interfacial membrane believed to be derived from the host cell plasma membrane. There, RPW8.2 activates a salicylic acid (SA) signaling-dependent defense strategy that concomitantly enhances the encasement of the haustorial complex and onsite accumulation of H(2)O(2), presumably for constraining the haustorium while reducing oxidative damage to the host cell. Targeting of RPW8.2 to the EHM, however, is SA independent and requires function of the actin cytoskeleton. Natural mutations that impair either defense activation or EHM targeting of RPW8.2 compromise the efficacy of RPW8.2-mediated resistance. Thus, the interception of haustoria is key for RPW8-mediated broad-spectrum mildew resistance.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Ascomicetos/crecimiento & desarrollo , Enfermedades de las Plantas/genética , Ácido Salicílico/metabolismo , Actinas/metabolismo , Arabidopsis/inmunología , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Citoesqueleto/metabolismo , ADN de Plantas/genética , Regulación de la Expresión Génica de las Plantas , Interacciones Huésped-Patógeno , Peróxido de Hidrógeno/metabolismo , Inmunidad Innata , Datos de Secuencia Molecular , Enfermedades de las Plantas/inmunología , Enfermedades de las Plantas/microbiología , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/inmunología , Plantas Modificadas Genéticamente/metabolismoRESUMEN
The RPW8 locus of Arabidopsis thaliana confers broad-spectrum resistance to powdery mildew pathogens. In many A. thaliana accessions, this locus contains two homologous genes, RPW8.1 and RPW8.2. In some susceptible accessions, however, these two genes are replaced by HR4, a homolog of RPW8.1. Here, we show that RPW8.2 from A. lyrata conferred powdery mildew resistance in A. thaliana, suggesting that RPW8.2 might have gained the resistance function before the speciation of A. thaliana and A. lyrata. To investigate how RPW8 has been maintained in A. thaliana, we examined the nucleotide sequence polymorphisms in RPW8 from 51 A. thaliana accessions, related disease reaction phenotypes to the evolutionary history of RPW8.1 and RPW8.2, and identified mutations that confer phenotypic variations. The average nucleotide diversities were high at RPW8.1 and RPW8.2, showing no sign of selective sweep. Moreover, we found that expression of RPW8 incurs fitness benefits and costs on A. thaliana in the presence and absence of the pathogens, respectively. Our results suggest that polymorphisms at the RPW8 locus in A. thaliana may have been maintained by complex selective forces, including those from the fitness benefits and costs both associated with RPW8.
Asunto(s)
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Genes de Plantas , Ascomicetos/patogenicidad , Secuencia de Bases , ADN de Plantas/genética , Evolución Molecular , Variación Genética , Datos de Secuencia Molecular , Fenotipo , Filogenia , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Plantas Modificadas Genéticamente , Homología de Secuencia de Ácido Nucleico , Factores de TiempoRESUMEN
The Resistance to Powdery Mildew 8 (RPW8) locus confers broad-spectrum resistance to powdery mildew in Arabidopsis thaliana. There are four Homologous to RPW8s (BrHRs) in Brassica rapa and three in Brassica oleracea (BoHRs). Brassica napus (Bn) is derived from diploidization of a hybrid between B. rapa and B. oleracea, thus should have seven homologs of RPW8 (BnHRs). It is unclear whether these genes are still maintained or lost in B. napus after diploidization and how they might have been evolved. Here, we reported the identification and sequence polymorphisms of BnHRs from a set of B. napus accessions. Our data indicated that while the BoHR copy from B. oleracea is highly conserved, the BrHR copy from B. rapa is relatively variable in the B. napus genome owing to multiple evolutionary events, such as gene loss, point mutation, insertion, deletion, and intragenic recombination. Given the overall high sequence homology of BnHR genes, it is not surprising that both intragenic recombination between two orthologs and two paralogs were detected in B. napus, which may explain the loss of BoHR genes in some B. napus accessions. When ectopically expressed in Arabidopsis, a C-terminally truncated version of BnHRa and BnHRb, as well as the full length BnHRd fused with YFP at their C-termini could trigger cell death in the absence of pathogens and enhanced resistance to powdery mildew disease. Moreover, subcellular localization analysis showed that both BnHRa-YFP and BnHRb-YFP were mainly localized to the extra-haustorial membrane encasing the haustorium of powdery mildew. Taken together, our data suggest that the duplicated BnHR genes might have been subjected to differential selection and at least some may play a role in defense and could serve as resistance resource in engineering disease-resistant plants.
RESUMEN
Powdery mildew fungi form feeding structures called haustoria inside epidermal cells of host plants to extract photosynthates for their epiphytic growth and reproduction. The haustorium is encased by an interfacial membrane termed the extrahaustorial membrane (EHM). The atypical resistance protein RPW8.2 from Arabidopsis is specifically targeted to the EHM where RPW8.2 activates haustorium-targeted (thus broad-spectrum) resistance against powdery mildew fungi. EHM-specific localization of RPW8.2 suggests the existence of an EHM-oriented protein/membrane trafficking pathway during EHM biogenesis. However, the importance of this specific trafficking pathway for host defense has not been evaluated via a genetic approach without affecting other trafficking pathways. Here, we report that expression of EHM-oriented, nonfunctional RPW8.2 chimeric proteins exerts dominant negative effect over functional RPW8.2 and potentially over other EHM-localized defense proteins, thereby compromising both RPW8.2-mediated and basal resistance to powdery mildew. Thus, our results highlight the importance of the EHM-oriented protein/membrane trafficking pathway for host resistance against haustorium-forming pathogens such as powdery mildew fungi.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Ascomicetos , Resistencia a la Enfermedad , Estructuras Fúngicas/metabolismo , Interacciones Huésped-Patógeno , Enfermedades de las Plantas/microbiología , Hojas de la Planta , Plantas Modificadas Genéticamente , Transporte de ProteínasRESUMEN
Sphingolipids comprise a major class of structural materials and lipid signaling molecules in all eukaryotic cells. Over the past two decades, there has been a phenomenal growth in the study of sphingolipids (i.e., sphingobiology) at an average rate of â¼1000 research articles per year. Sphingolipid studies in plants, though accounting for only a small fraction (â¼6%) of the total number of publications, have also enjoyed proportionally rapid growth in the past decade. Concomitant with the growth of sphingobiology, there has also been tremendous progress in our understanding of the molecular mechanisms of plant innate immunity. In this review, we (i) cross examine and analyze the major findings that establish and strengthen the intimate connections between sphingolipid metabolism and plant programmed cell death (PCD) associated with plant defense or disease; (ii) highlight and compare key bioactive sphingolipids involved in the regulation of plant PCD and possibly defense; (iii) discuss the potential role of sphingolipids in polarized membrane/protein trafficking and formation of lipid rafts as subdomains of cell membranes in relation to plant defense; and (iv) where possible, attempt to identify potential parallels for immunity-related mechanisms involving sphingolipids across kingdoms.
RESUMEN
Numerous fungal and oomycete pathogens penetrate the plant cell wall and extract nutrition from the host cells by a feeding structure called the haustorium. We recently revealed that the Arabidopsis resistance protein RPW8.2 is specifically targeted to the extrahaustorial membrane (EHM) for activation of haustorium-targeted resistance to powdery mildew pathogens. Consistent with its EHM-localization, RPW8.2 contains a putative transmembrane (TM) domain at its N-terminus. Here, we show that translational fusion of YFP to the N-terminus of RPW8.2 results in localization of YFP-RPW8.2 to both the plasma membrane and the EHM, and loss of RPW8.2's defense function. We also show that deletion of the TM domain results in mis-localization of the RPW8.2-YFP fusion protein and extremely low levels of accumulation. These results indicate that an intact N-terminal TM domain is necessary for EHM-specific localization and defense function of RPW8.2. In addition, we show that when expressed from the strong constitutive 35S viral promoter, RPW8.2 accumulates at low levels in the EHM insufficient to activate resistance, highlighting the importance of stronger spatiotemporal expression of RPW8.2 from its native promoter. Taken together, our results indicate that accurate and adequate spatiotemporal expression and localization of RPW8.2 is key to activation of resistance at the host-pathogen interface.