The scientific publication score - a new tool for summarizing evidence and data quality criteria of biomedical publications.

The number of biomedical research articles increases by over 2.5 million publications each year, making it difficult to stay up to date. In this study, we introduce a standardized search and evaluation tool to combat this issue. Employing crowdsourcing, a large database of publications is gathered. Using a standardized data entry format, coined the "scientific publication score" (SPS), specific publication results can be easily aggregated, thereby allowing fast and accurate comparisons for clinical questions. The SPS combines two quality dimensions. The first captures the quality of evidence of the study using the evidence criteria defined by the Centre for Evidence-Based Medicine, Oxford, UK. The second is more fine-grained and considers the magnitude of statistical analyses on individual and specific results. From 2014 to 2019, experts of the European Bone and Joint Infection Society (EBJIS) were asked to enter data of relevant publications about prosthetic joint infection. Data and evidence levels of specific results were averaged, summarized and ranked. A total of 366 publications were divided into two groups: (I) risk factors (e.g., host-related factors, pre- and postoperative issues) with 243 publications and (II) diagnostic methods (e.g., laboratory tests, imaging methods) with 123 publications. After ranking, the highest score for risk factors of prosthetic joint infection were calculated by the SPS for anemia (mean 3.50 ±  SD 0.91), malignancy (mean 3.17 ±  SD 0.29) and previous alloarthroplasty (mean 3.00 ±  SD 0.35). A comparison of the full SPS ranking with the ranking determined at the 2018 International Consensus Meeting (ICM) on Musculoskeletal Infection resulted in a Spearman rank correlation coefficient of 0.48 and a p  value of 0.0382. The diagnostic methods ranked highest by the SPS were aspirate leucocyte count (mean 3.15 ±  SD 1.21), interleukin 6 (mean 3.14 ±  SD 1.07) and aspirate (neutrophils over 80 %) (mean 3.12 ±  SD 0.63). The comparison to the ICM ranking yielded a Spearman rank correlation coefficient of 0.91 and a p  value of 0.0015. Our pilot study evaluated a new tool for the quality assessment of specific results based on the quality of the source publication. The SPS is suitable for a ranking of specific results by evidence and data quality criteria important for systematic reviews.

The Analysis of Bacterial Results in the Kidney Perfusion Fluid and Bone Allografts in Human Multiorgan Donors.

In 19 young human multiorgan donors, we simultaneously analyzed the bacterial contamination of the kidney perfusion fluid and all retrieved bone allografts. Donor exclusion criteria were done according to the American and European Association of Tissue Banks excluding all patients with perforating wounds. The kidney perfusate revealed a contamination in 17 of 19 (89.5%) donors. Allograft testing demonstrated positive bacterial growth in 34 of 76 allografts (44.7%). Microorganisms originated from the normal skin flora and could be related to contamination during the harvesting procedure. In 5 cases we cultured identical bacterial subspecies in both cultures as a possible sign for systemic bacterial spreading during the multiorgan harvesting procedure.

Strain adaptive bone remodelling in total joint replacement.

Histomorphologic analyses of artificial joint components implanted into bone need special technology for processing and for documentation; published histological work systematically done therefore is rare. The histopathology, three-dimensionally analyzed in a complete sequence of sections is, however, the only precise answer in terms of biocompatibility and bone response. A complete analysis allows a type-related predictable prognosis of an implantation that is at least comparable to a finite element analysis with respect to load transfer to host bone. The histopathologic collection of the ZOW Munich is comprised of more than 5000 nondemineralized bone and joint specimens and more than 500 artificial joint components implanted in the human skeleton for up to 25 years. Fifty-nine implant-bone specimens without signs of loosening already have been processed and analyzed systematically. According to the strain-adapted bone remodelling, different types of anchorage clearly were differentiated and their morphologic substrate could be worked out. Based on that, the cemented standard anchorage could be distinguished histologically from the cemented press-fit procedure, and the noncemented press-fit from the porous ingrowths pattern. In terms of the topography of the bony integration, the proximal and distal press-fit and ingrowth pattern were analyzed; beside that, the cemented and noncemented epiphyseal resurfacings could be defined histologically. In all histologic specimens the remodelling appeared as a result of stress-related strain, reflecting stiffness of the implant and the resistance of bone to deformation. It clearly was worked out that all success of cemented components is based on preserved cancellous bone honeycombs stiffened by bone cement, representing an adaptation of bone in terms of stiffness to the stiff implants.

Good preservation of initial mechanical properties in lipid-extracted, disinfected, freeze-dried sheep patellar tendon grafts.

Patellar tendon allografts can be used for anterior cruciate reconstruction to avoid morbidity of autografts on the donor side. Secondary processing of allografts is important for reducing immunological reactions, bacterial contamination and improving storage. We analyzed the effects of processing on the mechanical properties of patellar tendon grafts in 20 sheep. Group I (n = 10) was deep-frozen at -80 degrees C. Group II (n = 10) was processed by a lipid extraction/ freeze-drying method, including iodoacetic acid disinfection. The contralateral tendons, freeze-dried by dehydration in a vacuum at -50 degrees C for 3 hours, served as controls. We measured failure stress: group I (53, SD 14 MPa), control (26, SD 15 MPa) (p = 0.04); group II (49, SD 13 MPa), control (28, SD 5 MPa) (p = 0.08). Failure strain, normalized stiffness, and energy to failure were similar in the groups. Our method of extended processing did not change the properties of the deep-frozen patellar tendons. Therefore in vivo experiments can be used when studying the effects of transplantation on mechanical properties.

Regeneration of the donor side after autogenous fibula transplantation in 53 patients: evaluation by dual x-ray absorptiometry.

We determined the degree of fibular regeneration at the donor site, using radiographs and dual x-ray absorptiometry, in 53 patients who underwent autogenous nonvascularized fibular transplantation for tumor reconstruction in long bones (mean follow-up 15 (3-26) years). Logistic regression was used to determine whether gender, age at transplantation, time since transplantation, bone mineral density (BMD), and length of the graft were associated with fibular regeneration. 26 patients had spontaneous complete bone regeneration. Younger age at transplant was the only predictor of fibular regeneration. In predicting fibular regeneration, sensitivity was 96% and specificity 74%, using 15 years of age as a cut-off. In the long-term follow-up, we found only gradual changes in the BMD and the values ranged from 24% to 217%. We found no correlations of bone mineral density with age, gender, length of the graft, or time since transplantation.