Added value and limitations of amyloid-PET imaging: review and analysis of selected cases of mild cognitive impairment and dementia.

Amyloid-positron emission tomography (PET) imaging of the brain detects elevated amyloid-beta (amyloid-ß) neuritic plaques in vivo, which can be helpful in appropriately selected cases of mild cognitive impairment (MCI) and dementia, when Alzheimer's disease remains a possible etiology, after a comprehensive clinical evaluation. We reviewed cases of cognitively impaired patients who underwent amyloid-PET imaging because of diagnostic uncertainty. Pre- and post-PET elements of diagnosis and management were first compared, to assess impact of scan results on clinical decision-making, and then an analysis of those decisions was undertaken in appropriate clinical situations, to delineate the added value and limitations of amyloid-PET imaging. The potential benefits and limitations of this diagnostic tool are important to understand in an era when the utility of such scans in clinical practice is evolving.

Assessment of slot sizes in self-ligating brackets using electron microscopy.

OBJECTIVE: To measure the slot dimensions of 0.022 inch self-ligating upper central incisor brackets from six manufacturers using electron microscopy, to compare the measured dimensions with the manufacturers' published dimensions, and to determine if the walls of the slots were parallel. MATERIALS: Six self-ligating upper central incisor brackets from four manufacturers (SmartClip and Clarity SL, 3M Unitek, Monrovia, CA, USA; Speed, Strite Industries Ontario, Canada; Damon MX, Ormco, Orange, CA, USA; In-Ovation R and In-Ovation C, Dentsply GAC, Bohemia NY, USA) were imaged with a scanning electron microscope and the slots heights measured. Intra-operator repeatability and accuracy were determined. RESULTS: All brackets had slot sizes that were significantly larger (p < 0.05) than the stated 0.022 inch. Speed brackets were 5.1 per cent larger (0.02311 inch) and the closest to the published dimension. The SmartClip brackets were 14.8 per cent larger (0.02526 inch) than the quoted slot size of 0.022 inch. In most brackets the distances between the slot walls was generally greater further from the bracket bases. CONCLUSIONS: The actual measurements of upper central incisor self-ligating brackets from six manufacturers were larger than the manufacturers' stated dimension, and the walls of the slots diverged from the bracket bases.

Branching of the p-nitrophenol (PNP) degradation pathway in burkholderia sp. Strain SJ98: Evidences from genetic characterization of PNP gene cluster.

Aerobic microbial degradation of p-nitrophenol (PNP) has been classically shown to proceed via 'Hydroquinone (HQ) pathway' in Gram-negative bacteria, whereas in Gram-positive PNP degraders it proceed via 'Benzenetriol (BT) pathway'. These pathways are characterized by the ring cleavage of HQ and BT as terminal aromatic intermediates respectively. Earlier reports on PNP degradation have indicated these pathways to be mutually exclusive. We report involvement of both 'HQ' and 'BT' ring cleavage pathways in PNP degradation by Burkholderia sp. strain SJ98. Genetic characterization of an ~41 Kb DNA fragment harboring PNP degradation gene cluster cloned and sequenced from strain SJ98 showed presence of multiple orfs including pnpC and pnpD which corresponded to previously characterized 'benzenetriol-dioxygenase (BtD)' and 'maleylacetate reductase (MaR)' respectively. This gene cluster also showed presence of pnpE1 and pnpE2, which shared strong sequence identity to cognate sub-units of 'hydroquinone dioxygenase' (HqD). Heterologous expression and biochemical characterization ascertained the identity of PnpE1 and PnpE2. In in vitro assay reconstituted heterotetrameric complex of PnpE1 and PnpE2 catalyzed transformation of hydroquinone (HQ) into corresponding hydroxymuconic semialdehyde (HMS) in a substrate specific manner. Together, these results clearly establish branching of PNP degradation in strain SJ98. We propose that strain SJ98 presents a useful model system for future studies on evolution of microbial degradation of PNP.