RESUMEN
Bioaerosols in the form of microscopic airborne particles pose pervasive risks to humans and livestock. As either fully active components (e.g., viruses, bacteria, and fungi) or as whole or part of inactive fragments, they are among the least investigated pollutants in nature. Their identification and quantification are essential to addressing related dangers and to establishing proper exposure thresholds. However, difficulties in the development (and selection) of detection techniques and an associated lack of standardized procedures make the sensing of bioaerosols challenging. Through a comprehensive literature search, this review examines the mechanisms of conventional and advanced bioaerosol detection methods. It also provides a roadmap for future research and development in the selection of suitable methodologies for bioaerosol detection. The development of sample collection and sensing technology make it possible for continuous and automated operation. However, intensive efforts should be put to overcome the limitations of current technology as most of the currently available options tend to suffer from lengthy sample acquisition times and/or nonspecificity of probe material.
Asunto(s)
Microbiología del Aire , Atmósfera , Aerosoles , Bacterias , Hongos , HumanosRESUMEN
The present paper reports the assembly and pesticide sensing application of a nanometal organic framework [Cd(atc)(H2O)2]n ('atc'=2-aminoterephthalic acid). The assembly of the NMOF film has been achieved by sequential dipping of a 2-aminobenzylamine (2-ABA) modified indium tin oxide (ITO) slide in organic linker 'atc' and metal ion 'Cd(2+)' solutions. The different structural and morphological characteristics of the NMOF thin film have been characterized. The availability of pendent -COOH functional groups on the assembled NMOF film is exploited to synthesize a pesticide immunosensor by conjugating the NMOF film with anti-parathion antibody. This immunosensor has been explored for the electrochemical impedance spectroscopy (EIS) based analysis of parathion in the concentration range of 0.1-20ng/mL. The proposed detection is specific with respect to other organophosphate compounds, e.g. malathion, paraoxon, fenitrothion, monochrotophos and dichlorovos. The proposed sensor shows the detection limit of 0.1ng/mL and it is applicable for analysis of parathion in a rice sample. The sensor's performance is validated by comparting the obtained results with gas chromatographic data.