RESUMEN
Severe droughts have caused widespread tree mortality across many forest biomes with profound effects on the function of ecosystems and carbon balance. Climate change is expected to intensify regional-scale droughts, focusing attention on the physiological basis of drought-induced tree mortality. Recent work has shown that catastrophic failure of the plant hydraulic system is a principal mechanism involved in extensive crown death and tree mortality during drought, but the multi-dimensional response of trees to desiccation is complex. Here we focus on the current understanding of tree hydraulic performance under drought, the identification of physiological thresholds that precipitate mortality and the mechanisms of recovery after drought. Building on this, we discuss the potential application of hydraulic thresholds to process-based models that predict mortality.
Asunto(s)
Sequías , Estrés Fisiológico/fisiología , Árboles/fisiología , Aclimatación/fisiología , Desastres Naturales , Árboles/anatomía & histología , Árboles/genética , Agua/metabolismo , Agua/fisiología , Xilema/metabolismoRESUMEN
Natural selection is an important driver of genetic and phenotypic differentiation between species. For species in which potential gene flow is high but realized gene flow is low, adaptation via natural selection may be a particularly important force maintaining species. For a recent radiation of New World desert shrubs (Encelia: Asteraceae), we use fine-scale geographic sampling and population genomics to determine patterns of gene flow across two hybrid zones formed between two independent pairs of species with parapatric distributions. After finding evidence for extremely strong selection at both hybrid zones, we use a combination of field experiments, high-resolution imaging, and physiological measurements to determine the ecological basis for selection at one of the hybrid zones. Our results identify multiple ecological mechanisms of selection (drought, salinity, herbivory, and burial) that together are sufficient to maintain species boundaries despite high rates of hybridization. Given that multiple pairs of Encelia species hybridize at ecologically divergent parapatric boundaries, such mechanisms may maintain species boundaries throughout Encelia.
Asunto(s)
Asteraceae/genética , Clima Desértico , Hibridación Genética , Selección Genética , Ecosistema , Flujo Génico , Aptitud Genética , Herbivoria , México , Salinidad , Agua , VientoRESUMEN
Many plant leaves have two layers of photosynthetic tissue: the palisade and spongy mesophyll. Whereas palisade mesophyll consists of tightly packed columnar cells, the structure of spongy mesophyll is not well characterized and often treated as a random assemblage of irregularly shaped cells. Using micro-computed tomography imaging, topological analysis, and a comparative physiological framework, we examined the structure of the spongy mesophyll in 40 species from 30 genera with laminar leaves and reticulate venation. A spectrum of spongy mesophyll diversity encompassed two dominant phenotypes: first, an ordered, honeycomblike tissue structure that emerged from the spatial coordination of multilobed cells, conforming to the physical principles of Euler's law; and second, a less-ordered, isotropic network of cells. Phenotypic variation was associated with transitions in cell size, cell packing density, mesophyll surface-area-to-volume ratio, vein density, and maximum photosynthetic rate. These results show that simple principles may govern the organization and scaling of the spongy mesophyll in many plants and demonstrate the presence of structural patterns associated with leaf function. This improved understanding of mesophyll anatomy provides new opportunities for spatially explicit analyses of leaf development, physiology, and biomechanics.
Asunto(s)
Células del Mesófilo , Hojas de la Planta , Tamaño de la Célula , Células del Mesófilo/fisiología , Fotosíntesis , Hojas de la Planta/fisiología , Microtomografía por Rayos XRESUMEN
The epiphytic resurrection-or desiccation-tolerant (DT)-fern Pleopeltis polypodioides can survive extreme desiccation and recover physiological activity within hours of rehydration. Yet, how epiphytic DT ferns coordinate between deterioration and recovery of their hydraulic and photosynthetic systems remains poorly understood. We examined the functional status of the leaf vascular system, chlorophyll fluorescence, and photosynthetic rate during desiccation and rehydration of P. polypodioides. Xylem tracheids in the stipe embolized within 3-4 h during dehydration. When the leaf and rhizome received water, tracheids refilled after â¼24 h, which occurred along with dramatic structural changes in the stele. Photosynthetic rate and chlorophyll fluorescence recovered to predesiccation values within 12 h of rehydration, regardless of whether fronds were connected to their rhizome. Our data show that the epiphytic DT fern P. polypodioides can utilize foliar water uptake to rehydrate the leaf mesophyll and recover photosynthesis despite a broken hydraulic connection to the rhizome.
Asunto(s)
Desecación , Polypodiaceae/metabolismo , Agua/metabolismoRESUMEN
Xylella fastidiosa (Xf) is the bacterial pathogen responsible for Pierce's Disease (PD) in grapevine (Vitis vinifera L.) and numerous diseases in agriculturally and ecologically important species. Current theory suggests that localized inoculations via insect feeding lead to bacterial spread through the xylem, reducing water transport capacity, leading to declines in productivity, and ultimately death. Yet, the underlying mechanisms of Xf-induced mortality are not fully understood. In this study, we documented the development of PD symptoms over 12-13 weeks postinoculation. Subsequently assessed photosynthetic capacity, starch storage, and stem hydraulics in four grapevine genotypes (two PD-resistant and two PD-susceptible), comparing those physiological changes to control plants. PD-susceptible genotypes showed a coordinated decline in photosynthesis, starch storage, and stem hydraulics, whereas Xf-inoculation led to no change in starch and stem hydraulics in the PD-resistant genotypes. Together these data support the idea of a link between loss of hydraulic conductivity due to tylosis production with a downstream photosynthetic decline and starch depletion in the PD-susceptible genotypes. Our data support the theory that hydraulic failure and carbon starvation underlie plant mortality resulting from PD.
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Vitis , Fotosíntesis , Enfermedades de las Plantas/microbiología , Almidón , Vitis/fisiología , XylellaRESUMEN
Leaves balance CO2 and radiative absorption while maintaining water transport to maximise photosynthesis. Related species with contrasting leaf anatomy can provide insights into inherent and stress-induced links between structure and function for commonly measured leaf traits for important crops. We used two walnut species with contrasting mesophyll anatomy to evaluate these integrated exchange processes under non-stressed and drought conditions using a combination of light microscopy, X-ray microCT, gas exchange, hydraulic conductance, and chlorophyll distribution profiles through leaves. Juglans regia had thicker palisade mesophyll, higher fluorescence in the palisade, and greater low-mesophyll porosity that were associated with greater gas-phase diffusion (gIAS ), stomatal and mesophyll (gm ) conductances and carboxylation capacity. More and highly-packed mesophyll cells and bundle sheath extensions (BSEs) in Juglans microcarpa led to higher fluorescence in the spongy and in proximity to the BSEs. Both species exhibited drought-induced reductions in mesophyll cell volume, yet the associated increases in porosity and gIAS were obscured by declines in biochemical activity that decreased gm . Inherent differences in leaf anatomy between the species were linked to differences in gas exchange, light absorption and photosynthetic capacity, and drought-induced changes in leaf structure impacted performance via imposing species-specific limitations to light absorption, gas exchange and hydraulics.
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Dióxido de Carbono , Desecación , Células del Mesófilo , Fotosíntesis , Hojas de la Planta/anatomía & histologíaRESUMEN
Maintaining high rates of photosynthesis in leaves requires efficient movement of CO2 from the atmosphere to the mesophyll cells inside the leaf where CO2 is converted into sugar. CO2 diffusion inside the leaf depends directly on the structure of the mesophyll cells and their surrounding airspace, which have been difficult to characterize because of their inherently three-dimensional organization. Yet faster CO2 diffusion inside the leaf was probably critical in elevating rates of photosynthesis that occurred among angiosperm lineages. Here we characterize the three-dimensional surface area of the leaf mesophyll across vascular plants. We show that genome size determines the sizes and packing densities of cells in all leaf tissues and that smaller cells enable more mesophyll surface area to be packed into the leaf volume, facilitating higher CO2 diffusion. Measurements and modelling revealed that the spongy mesophyll layer better facilitates gaseous phase diffusion while the palisade mesophyll layer better facilitates liquid-phase diffusion. Our results demonstrate that genome downsizing among the angiosperms was critical to restructuring the entire pathway of CO2 diffusion into and through the leaf, maintaining high rates of CO2 supply to the leaf mesophyll despite declining atmospheric CO2 levels during the Cretaceous.
Asunto(s)
Dióxido de Carbono , Células del Mesófilo , Tamaño de la Célula , Tamaño del Genoma , Fotosíntesis , Hojas de la PlantaRESUMEN
Global warming is expected to dramatically accelerate forest mortality as temperature and drought intensity increase. Predicting the magnitude of this impact urgently requires an understanding of the process connecting atmospheric drying to plant tissue damage. Recent episodes of forest mortality worldwide have been widely attributed to dry conditions causing acute damage to plant vascular systems. Under this scenario vascular embolisms produced by water stress are thought to cause plant death, yet this hypothetical trajectory has never been empirically demonstrated. Here we provide foundational evidence connecting failure in the vascular network of leaves with tissue damage caused during water stress. We observe a catastrophic sequence initiated by water column breakage under tension in leaf veins which severs local leaf tissue water supply, immediately causing acute cellular dehydration and irreversible damage. By highlighting the primacy of vascular network failure in the death of leaves exposed to drought or evaporative stress our results provide a strong mechanistic foundation upon which models of plant damage in response to dehydration can be confidently structured.
Asunto(s)
Transpiración de Plantas , Xilema , Deshidratación , Sequías , Hojas de la PlantaRESUMEN
Leaf habit is a major axis of plant diversity that has consequences for carbon balance since the leaf is the primary site of photosynthesis. Nonstructural carbohydrates (NSCs) produced by photosynthesis can be allocated to storage and serve as a resiliency mechanism to future abiotic and biotic stress. However, how leaf habit affects NSC storage in an evolutionary context has not been shown. Using a comparative physiological framework and an analysis of evolutionary model fitting, we examined if variation in NSC storage is explained by leaf habit. We measured sugar and starch concentrations in 51 oak species (Quercus spp.) growing in a common garden and representing multiple evolutions of three different leaf habits (deciduous, brevideciduous and evergreen). The best fitting evolutionary models indicated that deciduous oak species are evolving towards higher NSC concentrations than their brevideciduous and evergreen relatives. Notably, this was observed for starch (the primary storage molecule) in the stem (a long-term C storage organ). Overall, our work provides insight into the evolutionary drivers of NSC storage and suggests that a deciduous strategy may confer an advantage against stress associated with a changing world. Future work should examine additional clades to further corroborate this idea.
Asunto(s)
Quercus , Metabolismo de los Hidratos de Carbono , Carbohidratos , Hojas de la Planta , ÁrbolesRESUMEN
There are multiple hypotheses for the spectacular plant diversity found in deserts. We explore how different factors, including the roles of ecological opportunity and selection, promote diversification and disparification in Encelia, a lineage of woody plants in the deserts of the Americas. Using a nearly complete species-level phylogeny based on double-digest restriction-aided sequencing along with a broad set of phenotypic traits, we estimate divergence times and diversification rates, identify instances of hybridization, quantify trait disparity and assess phenotypic divergence across environmental gradients. We show that Encelia originated and diversified recently (mid-Pleistocene) and rapidly, with rates comparable to notable adaptive radiations in plants. Encelia probably originated in the hot deserts of North America, with subsequent diversification across steep environmental gradients. We uncover multiple instances of gene flow between species. The radiation of Encelia is characterized by fast rates of phenotypic evolution, trait lability and extreme disparity across environments and between species pairs with overlapping geographic ranges. Encelia exemplifies how interspecific gene flow in combination with high trait lability can enable exceptionally fast diversification and disparification across steep environmental gradients.
Asunto(s)
Asteraceae , Hibridación Genética , Evolución Biológica , Flujo Génico , América del Norte , FilogeniaRESUMEN
Structural changes during severe drought stress greatly modify the hydraulic properties of fine roots. Yet, the physiological basis behind the restoration of fine root water uptake capacity during water recovery remains unknown. Using neutron radiography (NR), X-ray micro-computed tomography (micro-CT), fluorescence microscopy, and fine root hydraulic conductivity measurements (Lpr ), we examined how drought-induced changes in anatomy and hydraulic properties of contrasting grapevine rootstocks are coupled with fine root growth dynamics during drought and return of soil moisture. Lacunae formation in drought-stressed fine roots was associated with a significant decrease in fine root Lpr for both rootstocks. However, lacunae formation occurred under milder stress in the drought-resistant rootstock, 110R. Suberin was deposited at an earlier developmental stage in fine roots of 101-14Mgt (i.e. drought susceptible), probably limiting cortical lacunae formation during mild stress. During recovery, we found that only 110R fine roots showed rapid re-establishment of elongation and water uptake capacity and we found that soil water status surrounding root tips differed between rootstocks as imaged with NR. These data suggest that drought resistance in grapevine rootstocks is associated with rapid re-establishment of growth and Lpr near the root tip upon re-watering by limiting competing sites along the root cylinder.
Asunto(s)
Sequías , Vitis , Meristema , Raíces de Plantas , Agua , Microtomografía por Rayos XRESUMEN
Measuring and modeling the spatial distribution of chlorophyll within the leaf is critical for understanding the relationship between leaf structure and carbon assimilation, for defining the relative investments in leaf tissues from the perspective of leaf economics theory, and for the emerging application of in silico carbon assimilation models. Yet, spatially resolved leaf chlorophyll distribution data are limited. Here, we used epi-illumination fluorescence microscopy to estimate relative chlorophyll concentration as a function of mesophyll depth for 57 plant taxa. Despite interspecific variation due to differences in leaf thickness, mesophyll palisade fraction, and presence of large intercellular airspaces, the spatial distribution of chlorophyll in laminar leaves was remarkably well conserved across diverse lineages (ferns, cycads, conifers, ginkgo, basal angiosperms, magnoliids, monocots, and eudicots) and growth habits (tree, shrub, herbaceous, annual, perennial, evergreen, and deciduous). In the typical leaf, chlorophyll content increased gradually as a function of depth, peaking deep within the mesophyll. This chlorophyll distribution pattern is likely coupled to adaxial and abaxial intraleaf light gradients, including the relative enrichment of green light in the lower leaf. Chlorophyll distribution for the typical leaf from our dataset was well represented by a simple mathematical model (R2 = 0.94). We present chlorophyll distribution data and model equations for many ecologically and commercially relevant species and plant functional types (defined according to chlorophyll profile similarity, clade, and leaf thickness). These findings represent an advancement toward more accurate photosynthesis modeling and increase our understanding of first principles in intraleaf physiology.
Asunto(s)
Clorofila/metabolismo , Células del Mesófilo/metabolismo , Microscopía Fluorescente/métodos , Hojas de la Planta/metabolismo , Plantas/metabolismo , Algoritmos , Análisis por Conglomerados , Luz , Modelos Biológicos , Fotosíntesis/fisiología , Fotosíntesis/efectos de la radiación , Filogenia , Hojas de la Planta/citología , Plantas/clasificación , Plantas/genética , Especificidad de la EspecieRESUMEN
Water discharge from stem internal storage compartments is thought to minimize the risk of vessel cavitation. Based on this concept, one would expect that water storage compartments involved in the buffering of xylem tensions empty before the onset of vessel cavitation under drought stress, and potentially refill after soil saturation. However, scant in vivo data exist that elucidate this localized spatiotemporal coupling. In this study on intact saplings of American chestnut (Castanea dentata), x-ray computed microtomography (microCT) showed that the xylem matrix surrounding vessels releases stored water and becomes air-filled either concurrent to or after vessel cavitation under progressive drought stress. Among annual growth rings, the xylem matrix of the current year remained largely water-filled even under severe drought stress. In comparison, microtomography images collected on excised stems showed that applied pressures of much greater than 0 MPa were required to induce water release from the xylem matrix. Viability staining highlighted that water release from the xylem matrix was associated primarily with emptying of dead fibers. Refilling of the xylem matrix and vessels was detected in intact saplings when the canopy was bagged and stem water potential was close to 0 MPa, and in leafless saplings over the winter period. In conclusion, this study indicates that the bulk of water stored in the xylem matrix is released after the onset of vessel cavitation, and suggests that capillary water contributes to overall stem water storage under drought but is not used primarily for the prevention of drought-induced vessel cavitation in this species.
Asunto(s)
Fagaceae/metabolismo , Agua/metabolismo , Xilema/fisiología , Fagaceae/anatomía & histología , Microtomografía por Rayos X , Xilema/anatomía & histología , Xilema/metabolismoRESUMEN
Drought decreases water transport capacity of leaves and limits gas exchange, which involves reduced leaf leaf hydraulic conductance (Kleaf) in both the xylem and outside-xylem pathways. Some literature suggests that grapevines are hyper-susceptible to drought-induced xylem embolism. We combined Kleaf and gas exchange measurements, micro-computed tomography of intact leaves, and spatially explicit modeling of the outside-xylem pathways to evaluate the role of vein embolism and Kleaf in the responses of two different grapevine cultivars to drought. Cabernet Sauvignon and Chardonnay exhibited similar vulnerabilities of Kleaf and gs to dehydration, decreasing substantially prior to leaf xylem embolism. Kleaf and gs decreased by 80% for both cultivars by Ψâleaf approximately -0.7 MPa and -1.2 MPa, respectively, while leaf xylem embolism initiated around Ψâleaf = -1.25 MPa in the midribs and little to no embolism was detected in minor veins even under severe dehydration for both cultivars. Modeling results indicated that reduced membrane permeability associated with a Casparian-like band in the leaf vein bundle sheath would explain declines in Kleaf of both cultivars. We conclude that during moderate water stress, changes in the outside-xylem pathways, rather than xylem embolism, are responsible for reduced Kleaf and gs. Understanding this mechanism could help to ensure adequate carbon capture and crop performance under drought.
Asunto(s)
Sequías , Embolia , Hojas de la Planta , Agua , Microtomografía por Rayos X , XilemaRESUMEN
PREMISE: The young seedling life stage is critical for reforestation after disturbance and for species migration under climate change, yet little is known regarding their basic hydraulic function or vulnerability to drought. Here, we sought to characterize responses to desiccation including hydraulic vulnerability, xylem anatomical traits, and impacts on other stem tissues that contribute to hydraulic functioning. METHODS: Larix occidentalis, Pseudotsuga menziesii, and Pinus ponderosa (all ≤6 weeks old) were imaged using x-ray computed microtomography during desiccation to assess seedling biomechanical responses with concurrently measured hydraulic conductivity (ks ) and water potential (Ψ) to assess vulnerability to xylem embolism formation and other tissue damage. RESULTS: In non-stressed samples for all species, pith and cortical cells appeared circular and well hydrated, but they started to empty and deform with decreasing Ψ which resulted in cell tearing and eventual collapse. Despite the severity of this structural damage, the vascular cambium remained well hydrated even under the most severe drought. There were significant differences among species in vulnerability to xylem embolism formation, with 78% xylem embolism in L. occidentalis by Ψ of -2.1 MPa, but only 47.7% and 62.1% in P. ponderosa and P. menziesii at -4.27 and -6.73 MPa, respectively. CONCLUSIONS: Larix occidentalis seedlings appeared to be more susceptible to secondary xylem embolism compared to the other two species, but all three maintained hydration of the vascular cambium under severe stress, which could facilitate hydraulic recovery by regrowth of xylem when stress is relieved.
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Pseudotsuga , Tracheophyta , Desecación , Sequías , Plantones , Agua , XilemaRESUMEN
Maintaining water balance has been a critical constraint shaping the evolution of leaf form and function. However, flowers, which are heterotrophic and relatively short-lived, may not be constrained by the same physiological and developmental factors. We measured physiological parameters derived from pressure-volume curves for leaves and flowers of 22 species to characterize the diversity of hydraulic traits in flowers and to determine whether flowers are governed by the same constraints as leaves. Compared with leaves, flowers had high saturated water content, which was a strong predictor of hydraulic capacitance in both leaves and flowers. Principal component analysis revealed that flowers occupied a different region of multivariate trait space than leaves and that hydraulic traits are more diverse in flowers than in leaves. Without needing to maintain high rates of transpiration, flowers rely on other hydraulic traits, such as high hydraulic capacitance, to maintain turgor pressure. As a result, instead of employing a metabolically expensive but durable carbon (C)-based skeleton, flowers may rely predominantly on a metabolically cheaper, hydrostatic skeleton to keep their structures on display for pollinators, which has important implications for both the costs of reproduction and the biomechanical performance of flowers, particularly during drought.
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Flores/fisiología , Hojas de la Planta/fisiología , Carácter Cuantitativo Heredable , Agua/fisiología , Análisis Multivariante , Ósmosis , Filogenia , Presión , Análisis de Componente Principal , Análisis de Regresión , Especificidad de la EspecieRESUMEN
The leaf intercellular airspace (IAS) is generally considered to have high conductance to CO2 diffusion relative to the liquid phase. While previous studies accounted for leaf-level variation in porosity and mesophyll thickness, they omitted 3D IAS traits that potentially influence IAS conductance (gIAS). Here, we reevaluated the standard equation for gIAS by incorporating tortuosity, lateral path lengthening, and IAS connectivity. We measured and spatially mapped these geometric IAS traits for 19 Bromeliaceae species with Crassulacean acid metabolism (CAM) or C3 photosynthetic pathways using x-ray microcomputed tomography imaging and a novel computational approach. We found substantial variation in porosity (0.04-0.73 m3 m-3), tortuosity (1.09-3.33 m2 m-2), lateral path lengthening (1.12-3.19 m m-1), and IAS connectivity (0.81-0.97 m2 m-2) across all bromeliad leaves. The revised gIAS model predicted significantly lower gIAS in CAM (0.01-0.19 mol m-2 s-1 bar-1) than in C3 (0.41-2.38 mol m-2 s-1 bar-1) plants due to a coordinated decline in these IAS traits. Our reevaluated equation also generally predicted lower gIAS values than the former one. Moreover, we observed high spatial heterogeneity in these IAS geometric traits throughout the mesophyll, especially within CAM leaves. Our data show that IAS traits that better capture the 3D complexity of leaves strongly influence gIAS and that the impact of the IAS on mesophyll conductance should be carefully considered with respect to leaf anatomy. We provide a simple function to estimate tortuosity and lateral path lengthening in the absence of access to imaging tools such as x-ray microcomputed tomography or other novel 3D image-processing techniques.
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Bromeliaceae/metabolismo , Dióxido de Carbono/metabolismo , Células del Mesófilo/metabolismo , Hojas de la Planta/metabolismo , Algoritmos , Bromeliaceae/clasificación , Bromeliaceae/genética , Difusión , Fotosíntesis , Filogenia , Hojas de la Planta/anatomía & histología , Hojas de la Planta/citología , Porosidad , Especificidad de la Especie , Microtomografía por Rayos XRESUMEN
The influence of the dynamics of leaf hydraulic conductance (K leaf) diurnally and during dehydration on stomatal conductance and photosynthesis remains unclear. Using the model species Arabidopsis (Arabidopsis thaliana ecotype Columbia-0), we applied a multitiered approach including physiological measurements, high-resolution x-ray microcomputed tomography, and modeling at a range of scales to characterize (1) K leaf decline during dehydration; (2) its basis in the hydraulic conductances of leaf xylem and outside-xylem pathways (K ox); (3) the dependence of its dynamics on irradiance; (4) its impact on diurnal patterns of stomatal conductance and photosynthetic rate; and (5) its influence on gas exchange and survival under simulated drought regimes. Arabidopsis leaves showed strong vulnerability to dehydration diurnally in both gas exchange and hydraulic conductance, despite lack of xylem embolism or conduit collapse above the turgor loss point, indicating a pronounced sensitivity of K ox to dehydration. K leaf increased under higher irradiance in well-hydrated leaves across the full range of water potential, but no shift in K leaf vulnerability was observed. Modeling indicated that responses to dehydration and irradiance are likely attributable to changes in membrane permeability and that a dynamic K ox would contribute strongly to stomatal closure, improving performance, survival, and efficient water use during drought. These findings for Columbia-0 provide a baseline for assessing variation across genotypes in hydraulic traits and their influence on gas exchange during dehydration.
Asunto(s)
Arabidopsis/fisiología , Dióxido de Carbono/metabolismo , Hojas de la Planta/fisiología , Ritmo Circadiano , Deshidratación , Sequías , Modelos Biológicos , Fotosíntesis , Microtomografía por Rayos X , Xilema/fisiologíaRESUMEN
BACKGROUND AND AIMS: Deciduous angiosperm trees transport xylem sap through trunks and branches in vessels within annual growth rings. Utilizing previous growth rings for sap transport could increase vessel network size and redundancy but may expose new xylem to residual air embolisms in the network. Despite the important role of vessel networks in sap transport and drought resistance, our understanding of cross-ring connections within and between species is limited. METHODS: We studied cross-ring connections in four temperate deciduous trees using dye staining and X-ray microcomputed tomography (microCT) to detect xylem connectivity across growth rings and quantify their impact on hydraulic conductivity. KEY RESULTS: Acer rubrum and Fraxinus americana had cross-ring connections visible in microCT but only A. rubrum used previous growth rings for axial sap flow. Fagus grandifolia and Quercus rubra, however, did not have cross-ring connections. Accounting for the number of growth rings that function for axial transport improved hydraulic conductivity estimates. CONCLUSIONS: These data suggest that the presence of cross-ring connections may help explain aspects of whole-tree xylem sap transport and should be considered for plant hydraulics measurements in these species and others with similar anatomy.
Asunto(s)
Transpiración de Plantas , Árboles , Agua , Microtomografía por Rayos X , XilemaRESUMEN
PREMISE: Water deficit and drought conditions are increasing in intensity, frequency, and duration in the Iberian Peninsula. We observed natural variation in leaf traits across the range of Quercus suber L. (cork oak), an ecologically important species within this region. Stomatal traits (e.g., pore length, maximum aperture) and carbon isotope composition (δ13 C) provide an opportunity to examine the integrative effects of drought and dry-season intensity on leaf development, maximum stomatal conductance, and adaptation to precipitation regimes. METHODS: Gross leaf traits (e.g., area, thickness), stomatal traits (e.g., pore length, size, aperture), and carbon isotope discrimination were measured in Q. suber leaves, and maximum stomatal conductance to water vapor (gwmax ) was calculated. Trees were sampled from nine natural populations across a climate gradient in the Iberian Peninsula, including trees from two genetic lineages. Linear mixed models compared total water deficit to leaf traits, accounting for tree and site as random effects. RESULTS: Quercus suber gross leaf morphology remained consistent across the climate gradient, but increasing water deficit was correlated with smaller stomata at the leaf level and low δ13 C at the tree level. No traits were significantly different between the two genetic lineages. CONCLUSIONS: While there were no significant differences in gross leaf morphology across the climate gradient or between the genetic lineages, stomatal traits and δ13 C responded to climate, suggesting that Q. suber can inhabit a range of environments in the Iberian Peninsula via micro-adjustments of the trait that controls water loss into the atmosphere.