Fungal survival in ensiled swine faeces.

The survival of several genera of fungi was determined in the ensiled solid fraction of swine faeces after 0, 7, 14, 28 and 56 days of ensiling. The experiment had two treatments, un-ensiled and ensiled manure, in a split-plot design. The manure was distributed into 50 containers; samples, taken at the specified times, were cultured in agar potato dextrose medium, incubated, and colony forming units (CFU/g) were counted and log-transformed. The ensiling process decreased the number of CFU after 56 days. Five fungal genera were identified (Absidia spp., Aspergillus spp., Penicillium spp., Rhizopus spp. and non-fructiferous fungi), and their vulnerability to the ensiling conditions varied, although most of them slowed their growth or disappeared after 14 days of ensiling.

Survival and viability of Ascaris suum and Oesophagostomum dentatum in ensiled swine faeces.

The survival and viability of eggs from Ascaris suum and Oesophagostomum dentatum and of infective larvae (L3) from O. dentatum were determined in the ensiled solid fraction of swine faeces after 0, 7, 14, 28 and 56 days of ensiling. The experiment had two treatments, un-ensiled and ensiled manure, in a split-plot design. Each of 50 containers was inoculated with 40,000 eggs of both A. suum and O. dentatum, and another 50 containers were inoculated with 32,747 L3 of O. dentatum each. A. suum eggs were not destroyed by the ensiling process, although their viability was diminished. O. dentatum eggs and larvae were destroyed during the first 7-14 days of the ensiling process.

The utilization of multidimensional scaling to identify forage characteristics associated with preference in sheep.

Forage preference is difficult to assess but is likely an important factor in determining DM intake. In two experiments, six sheep (wethers, Exp. 1; ewes, Exp. 2) were used to obtain preference ratings on nine hays (Exp. 1) or nine fresh forages (Exp. 2). The statistical procedure of multidimensional scaling was used to develop orthogonal dimensions to account for the observed variation in preference for hays and fresh forages. This analysis produced three dimensions that accounted for 54% of the variation in mean 3-d preference for hays (Exp. 1). The three dimensions were subsequently associated by regression analysis with seven compositional variables. These were IVDMD and concentrations of monosaccharides, short-chain polysaccharides (SCP), and disaccharides in dimension 1(R2 = .99); acid detergent insoluble ash (ADIA) concentration, monosaccharide and SCP concentrations, and masticate NDF in dimension 2 (R2 = .99); and disaccharide and starch concentrations in dimension 3 (R2 = .65). Three dimensions accounting for 51% of the variation in fresh forage preference were found and related to four variables by regression analysis. These were disaccharide concentration and median particle size in dimension 1 (R2 = .76) and starch and monosaccharide + disaccharide concentrations in dimension 3 (R2 = .97). No physicochemical variables analyzed in this study were found to be significantly associated with the coordinates for the forages in dimension 2. Multidimensional scaling accounted for more than half the observed variation in animal preference and, in all but one case, these dimensions could be associated with physicochemical characteristics of the forages.

Evaluation of the Captec chrome controlled-release device for the estimation of fecal output by grazing sheep.

Three grazing experiments were conducted to compare actual fecal output (FO) by sheep, determined with fecal collection bags, to fecal output predicted using Captec chromic oxide (Cr2O3) controlled-release capsules. In Exp. 1, 14 crossbred wethers dosed with controlled-release Cr2O3 capsules rotationally grazed four paddocks of alfalfa (Medicago sativa L.) for 18 d. Total fecal collection and grab samples were taken daily from each animal; feces were dried and assayed for Cr. From d 5 to 12 after dosing, mean actual FO (429 +/- 9.6 g of DM/d) differed (P less than .001) from FO predicted by the capsule (463 +/- 12.8 g of DM/d). The correlation between actual and predicted FO was r = .59. When data were averaged by day, the correlation increased to .82. In Exp. 2, Tifton 44 bermudagrass (Cynodon dactylon [L.] Pers.) was strip-grazed for 30 d by the same 14 wethers used in Exp. 1. Mean actual FO (369 +/- 5.1 g of DM/d) differed (P less than .0001) from predicted FO (415 +/- 9.3 g of DM/d), with a correlation of .60. In Exp. 3, 72 crossbred lambs were blocked by weight (light and heavy) and assigned to six groups of eight wethers (four with fecal bags) and four ewes, allotted randomly to three stocking rates (74, 99, and 148 sheep/ha). Sampling occurred from d 6 to 10 after dosing (Period 1) and d 20 to 24 after dosing (Period 2).(ABSTRACT TRUNCATED AT 250 WORDS)