RESUMEN
A chiral sensor with optical rotation detection based on weak measurement for the kinetic study of sucrose hydrolysis is presented. Based on the polarization modulation to the pre-selection state, the optical rotation of chiral sample was accurately determined through the central wavelength shift of the output spectrum. With this approach, the concentration response curves of sucrose and its hydrolysis products, i.e., fructose and glucose, were experimentally obtained for the hydrolysis analysis. By collecting the output spectrum with a frequency of 100 Hz and fitting the central wavelength shift synchronously during the measurement, the sucrose hydrolysis process was monitored in real time. Different hydrolysis conditions with varied concentration of invertase enzyme and citrate were implemented in this work. As a consequence, the real-time hydrolysis curves of the hydrolysis process with distinct velocities was achieved and analyzed. Such a kinetic monitoring about sucrose hydrolysis with optical rotation detection technology played a critical role in the researches involving sucrose, and also revealed the great potential of weak measurement in intersections, such as food safety inspection and chemical analysis.
RESUMEN
With the structure of biprism, polarization beam splitting in a Glan-Taylor polarizer was explored based on the birefringence and Goos-Hanchen shift. Due to the birefringence of the light in calcite crystal, the extraordinary light worked as the position calibration. As for the ordinary light, the propagated direction tilted noticeably due to the refraction as well as Goos-Hanchen effect at the prism-air interface of in the air gap. The Snell's law and stationary-phase approach were utilized for the calculation of the beam splitting between the two orthogonal polarization elements. By choosing appropriate incident angle and initial polarization, remarkable beam splitting was realized. With this configuration, the resolution with a magnitude of 10 - 5 ° and 10 - 3 ° was achieved for the response of the incident angle and polarization detection respectively.