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Cancer remains a significant global health challenge, with traditional therapies like surgery, chemotherapy, and radiation often accompanied by systemic toxicity and damage to healthy tissues. Despite progress in treatment, these approaches have limitations such as non-specific targeting, systemic toxicity, and resistance development in cancer cells. In recent years, nanotechnology has emerged as a revolutionary frontier in cancer therapy, offering potential solutions to these challenges. Nanoparticles, due to their unique physical and chemical properties, can carry therapeutic payloads, navigate biological barriers, and selectively target cancer cells. Metal-based nanoparticles, in particular, offer unique properties suitable for various therapeutic applications. Recent advancements have focused on the integration of metal-based nanoparticles to enhance the efficacy and precision of photodynamic therapy. Integrating nanotechnology into cancer therapy represents a paradigm shift, enabling the development of strategies with enhanced specificity and reduced off-target effects. This review aims to provide a comprehensive understanding of the pivotal role of metal-based nanoparticles in photodynamic therapy. We explore the mechanisms, biocompatibility, and applications of metal-based nanoparticles in photodynamic therapy, highlighting the challenges and the limitations in their use, as well as the combining of metal-based nanoparticles/photodynamic therapy with other strategies as a synergistic therapeutic approach for cancer treatment.
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Magnetic nanocomposites (MNC) are promising theranostic platforms with tunable physicochemical properties allowing for remote drug delivery and multimodal imaging. Here, we developed doxorubicin-loaded Fe3O4-Au MNC (DOX-MNC) using electron beam physical vapor deposition (EB-PVD) in combination with magneto-mechanochemical synthesis to assess their antitumor effect on Walker-256 carcinosarcoma under the influence of a constant magnetic (CMF) and electromagnetic field (EMF) by comparing tumor growth kinetics, magnetic resonance imaging (MRI) scans and electron spin resonance (ESR) spectra. Transmission (TEM) and scanning electron microscopy (SEM) confirmed the formation of spherical magnetite nanoparticles with a discontinuous gold coating that did not significantly affect the ferromagnetic properties of MNC, as measured by vibrating-sample magnetometry (VSM). Tumor-bearing animals were divided into the control (no treatment), conventional doxorubicin (DOX), DOX-MNC and DOX-MNC + CMF + EMF groups. DOX-MNC + CMF + EMF resulted in 14% and 16% inhibition of tumor growth kinetics as compared with DOX and DOX-MNC, respectively. MRI visualization showed more substantial tumor necrotic changes after the combined treatment. Quantitative analysis of T2-weighted (T2W) images revealed the lowest value of skewness and a significant increase in tumor intensity in response to DOX-MNC + CMF + EMF as compared with the control (1.4 times), DOX (1.6 times) and DOX-MNC (1.8 times) groups. In addition, the lowest level of nitric oxide determined by ESR was found in DOX-MNC + CMF + EMF tumors, which was close to that of the muscle tissue in the contralateral limb. We propose that the reason for the relationship between the observed changes in MRI and ESR is the hyperfine interaction of nuclear and electron spins in mitochondria, as a source of free radical production. Therefore, these results point to the use of EB-PVD and magneto-mechanochemically synthesized Fe3O4-Au MNC loaded with DOX as a potential candidate for cancer magnetic nanotheranostic applications.
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Objective.Detectors that can provide accurate dosimetry for microbeam radiation therapy (MRT) must possess intrinsic radiation hardness, a high dynamic range, and a micron-scale spatial resolution. In this work we characterize hydrogenated amorphous silicon detectors for MRT dosimetry, presenting a novel combination of flexible, ultra-thin and radiation-hard features.Approach.Two detectors are explored: an n-type/intrinsic/p-type planar diode (NIP) and an NIP with an additional charge selective layer (NIP + CSC).Results.The sensitivity of the NIP + CSC detector was greater than the NIP detector for all measurement conditions. At 1 V and 0 kGy under the 3T Cu-Cu synchrotron broadbeam, the NIP + CSC detector sensitivity of (7.76 ± 0.01) pC cGy-1outperformed the NIP detector sensitivity of (3.55 ± 0.23) pC cGy-1by 219%. The energy dependence of both detectors matches closely to the attenuation coefficient ratio of silicon against water. Radiation damage measurements of both detectors out to 40 kGy revealed a higher radiation tolerance in the NIP detector compared to the NIP + CSC (17.2% and 33.5% degradations, respectively). Percentage depth dose profiles matched the PTW microDiamond detector's performance to within ±6% for all beam filtrations except in 3T Al-Al due to energy dependence. The 3T Cu-Cu microbeam field profile was reconstructed and returned microbeam width and peak-to-peak values of (51 ± 1)µm and (405 ± 5)µm, respectively. The peak-to-valley dose ratio was measured as a function of depth and agrees within error to the values obtained with the PTW microDiamond. X-ray beam induced charge mapping of the detector revealed minimal dose perturbations from extra-cameral materials.Significance.The detectors are comparable to commercially available dosimeters for quality assurance in MRT. With added benefits of being micron-sized and possessing a flexible water-equivalent substrate, these detectors are attractive candidates for quality assurance,in-vivodosimetry and in-line beam monitoring for MRT and FLASH therapy.
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Radiometría , Silicio , Silicio/química , Radiometría/instrumentación , Hidrógeno , Radioterapia/instrumentaciónRESUMEN
BACKGROUND: The increasing use of complex and high dose-rate treatments in radiation therapy necessitates advanced detectors to provide accurate dosimetry. Rather than relying on pre-treatment quality assurance (QA) measurements alone, many countries are now mandating the use of in vivo dosimetry, whereby a dosimeter is placed on the surface of the patient during treatment. Ideally, in vivo detectors should be flexible to conform to a patient's irregular surfaces. PURPOSE: This study aims to characterize a novel hydrogenated amorphous silicon (a-Si:H) radiation detector for the dosimetry of therapeutic x-ray beams. The detectors are flexible as they are fabricated directly on a flexible polyimide (Kapton) substrate. METHODS: The potential of this technology for application as a real-time flexible detector is investigated through a combined dosimetric and flexibility study. Measurements of fundamental dosimetric quantities were obtained including output factor (OF), dose rate dependence (DPP), energy dependence, percentage depth dose (PDD), and angular dependence. The response of the a-Si:H detectors investigated in this study are benchmarked directly against commercially available ionization chambers and solid-state diodes currently employed for QA practices. RESULTS: The a-Si:H detectors exhibit remarkable dose linearities in the direct detection of kV and MV therapeutic x-rays, with calibrated sensitivities ranging from (0.580 ± 0.002) pC/cGy to (19.36 ± 0.10) pC/cGy as a function of detector thickness, area, and applied bias. Regarding dosimetry, the a-Si:H detectors accurately obtained OF measurements that parallel commercially available detector solutions. The PDD response closely matched the expected profile as predicted via Geant4 simulations, a PTW Farmer ionization chamber and a PTW ROOS chamber. The most significant variation in the PDD performance was 5.67%, observed at a depth of 3 mm for detectors operated unbiased. With an external bias, the discrepancy in PDD response from reference data was confined to ± 2.92% for all depths (surface to 250 mm) in water-equivalent plastic. Very little angular dependence is displayed between irradiations at angles of 0° and 180°, with the most significant variation being a 7.71% decrease in collected charge at a 110° relative angle of incidence. Energy dependence and dose per pulse dependence are also reported, with results in agreement with the literature. Most notably, the flexibility of a-Si:H detectors was quantified for sample bending up to a radius of curvature of 7.98 mm, where the recorded photosensitivity degraded by (-4.9 ± 0.6)% of the initial device response when flat. It is essential to mention that this small bending radius is unlikely during in vivo patient dosimetry. In a more realistic scenario, with a bending radius of 15-20 mm, the variation in detector response remained within ± 4%. After substantial bending, the detector's photosensitivity when returned to a flat condition was (99.1 ± 0.5)% of the original response. CONCLUSIONS: This work successfully characterizes a flexible detector based on thin-film a-Si:H deposited on a Kapton substrate for applications in therapeutic x-ray dosimetry. The detectors exhibit dosimetric performances that parallel commercially available dosimeters, while also demonstrating excellent flexibility results.
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Radiometría , Silicio , Radiometría/instrumentación , Hidrógeno , Dosimetría in Vivo , Terapia por Rayos X/instrumentación , HumanosRESUMEN
Objective. Microbeam radiation therapy (MRT) is an alternative emerging radiotherapy treatment modality which has demonstrated effective radioresistant tumour control while sparing surrounding healthy tissue in preclinical trials. This apparent selectivity is achieved through MRT combining ultra-high dose rates with micron-scale spatial fractionation of the delivered x-ray treatment field. Quality assurance dosimetry for MRT must therefore overcome a significant challenge, as detectors require both a high dynamic range and a high spatial resolution to perform accurately.Approach. In this work, a series of radiation hard a-Si:H diodes, with different thicknesses and carrier selective contact configurations, have been characterised for x-ray dosimetry and real-time beam monitoring applications in extremely high flux beamlines utilised for MRT at the Australian Synchrotron.Results. These devices displayed superior radiation hardness under constant high dose-rate irradiations on the order of 6000 Gy s-1, with a variation in response of 10% over a delivered dose range of approximately 600 kGy. Dose linearity of each detector to x-rays with a peak energy of 117 keV is reported, with sensitivities ranging from (2.74 ± 0.02) nC/Gy to (4.96 ± 0.02) nC/Gy. For detectors with 0.8µm thick active a-Si:H layer, their operation in an edge-on orientation allows for the reconstruction of micron-size beam profiles (microbeams). The microbeams, with a nominal full-width-half-max of 50µm and a peak-to-peak separation of 400µm, were reconstructed with extreme accuracy. The full-width-half-max was observed as 55 ± 1µm. Evaluation of the peak-to-valley dose ratio and dose-rate dependence of the devices, as well as an x-ray induced charge (XBIC) map of a single pixel is also reported.Significance. These devices based on novel a-Si:H technology possess a unique combination of accurate dosimetric performance and radiation resistance, making them an ideal candidate for x-ray dosimetry in high dose-rate environments such as FLASH and MRT.
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Silicio , Sincrotrones , Rayos X , Australia , Radiometría/métodosRESUMEN
Art objects form an essential part of cultural heritage and are appreciated for their artistic values. However, the observed investment in art and capacity for high monetary returns encourages counterfeiting of art objects. The art market's lack of transparency and traditional confidential protocols amplifies the problem. Radiocarbon analysis provides a tool to detect anachronistic materials. Measurement of bomb peak radiocarbon, which was observed in the atmosphere during the last 70 years, can provide clear evidence of post-1950 material. Here we briefly introduce the method and discuss its application in detecting forgeries. Three accelerator mass spectrometry AMS laboratories performed a 14C dating inter-comparison study on the material used in art. Results obtained on modern cotton paper, two antique sheets of paper, one parchment, and one textile demonstrate the radiocarbon dating capacity to date the material accurately. The excellent agreement between laboratories is crucial for the broader application of this scientific tool in forensic studies and court cases.
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Arte , Datación Radiométrica , Radioisótopos de Carbono/análisis , Espectrometría de Masas , Datación Radiométrica/métodosRESUMEN
The Bologna Archaeological Museum, in cooperation with prestigious Italian universities, institutions, and independent scholars, recently began a vast investigation programme on a group of Egyptian coffins of Theban provenance dating to the first millennium BC, primarily the 25th-26th Dynasty (c. 746-525 BC). Herein, we present the results of the multidisciplinary investigation carried out on one of these coffins before its restoration intervention: the anthropoid wooden coffin of Un-Montu (Inv. MCABo EG1960). The integration of radiocarbon dating, wood species identification, and CT imaging enabled a deep understanding of the coffin's wooden structure. In particular, we discuss the results of the tomographic investigation performed in situ. The use of a transportable X-ray facility largely reduced the risks associated with the transfer of the large object (1.80 cm tall) out of the museum without compromising image quality. Thanks to the 3D tomographic imaging, the coffin revealed the secrets of its construction technique, from the rational use of wood to the employment of canvas (incamottatura), from the use of dowels to the assembly procedure.
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Posidonia oceanica is a seagrass endemic to the Mediterranean and it has been widely used as a bioindicator. We studied the layers of a 500-year-old matte using a multiproxy approach (δ13C, δ15N, 14C and C and N concentrations in seagrass debris) in order to evaluate the potential of P. oceanica as a long-term environmental indicator of N pollution and CO2 emissions. From 1581 to 1800, accumulation rate was ca. 0.35 cm year-1, while in the last 100 years it has amounted to ca. 0.51 cm year-1. We observed increasing δ15N values with height in the vertical matte profile, indicating an increase in anthropogenic organic N inputs over time. In contrast, no clear trend in the δ13C values was observed. This study reconstructs the long-term impact of human activities on a seagrass meadow located off the Italian coast, yielding long-term background information that can help managers to implement efficient plans.
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Alismatales , Humanos , Mar Mediterráneo , NutrientesRESUMEN
In this paper, by means of high-resolution photoemission, soft X-ray absorption and atomic force microscopy, we investigate, for the first time, the mechanisms of damaging, induced by neutron source, and recovering (after annealing) of p-i-n detector devices based on hydrogenated amorphous silicon (a-Si:H). This investigation will be performed by mean of high-resolution photoemission, soft X-Ray absorption and atomic force microscopy. Due to dangling bonds, the amorphous silicon is a highly defective material. However, by hydrogenation it is possible to reduce the density of the defect by several orders of magnitude, using hydrogenation and this will allow its usage in radiation detector devices. The investigation of the damage induced by exposure to high energy irradiation and its microscopic origin is fundamental since the amount of defects determine the electronic properties of the a-Si:H. The comparison of the spectroscopic results on bare and irradiated samples shows an increased degree of disorder and a strong reduction of the Si-H bonds after irradiation. After annealing we observe a partial recovering of the Si-H bonds, reducing the disorder in the Si (possibly due to the lowering of the radiation-induced dangling bonds). Moreover, effects in the uppermost coating are also observed by spectroscopies.
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All-inorganic cesium lead bromine (CsPbBr3) perovskites have gained a tremendous potential in optoelectronics due to interesting photophysical properties and much better stability than the hybrid counterparts. Although pulsed laser deposition (PLD) is a promising alternative to solvent-based and/or thermal deposition approaches due to its versatility in depositing multi-elemental materials, deep understanding of the implications of both target composition and PLD mechanisms on the properties of CsPbBr3 films is still missing. In this paper, we deal with thermally assisted preparation of mechano-chemically synthesized CsPbBr3 ablation targets to grow CsPbBr3 films by PLD at the fluence 2 J/cm2. We study both Cs rich- and stoichiometric PbBr2-CsBr mixture-based ablation targets and point out compositional deviations of the associated films resulting from the mass distribution of the PLD-generated plasma plume. Contrary to the conventional meaning that PLD guarantees congruent elemental transfer from the target to the substrate, our study demonstrates cation off-stoichiometry of PLD-grown CsPbBr3 films depending on composition and thermal treatment of the ablation target. The implications of the observed enrichment in the heavier element (Pb) and deficiency in the lighter element (Br) of the PLD-grown films are discussed in terms of optical response and with the perspective of providing operative guidelines and future PLD-deposition strategies of inorganic perovskites.
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All crops are the product of a domestication process that started less than 12,000 years ago from one or more wild populations1,2. Farmers selected desirable phenotypic traits (such as improved energy accumulation, palatability of seeds and reduced natural shattering3) while leading domesticated populations through several more or less gradual demographic contractions2,4. As a consequence, the erosion of wild genetic variation5 is typical of modern cultivars, making them highly susceptible to pathogens, pests and environmental change6,7. The loss of genetic diversity hampers further crop improvement programmes to increase food production in a changing world, posing serious threats to food security8,9. Using both ancient and modern seeds, we analysed the temporal dynamics of genetic variation and selection during the domestication process of the common bean (Phaseolus vulgaris) in the southern Andes. Here, we show that most domestic traits were selected for before 2,500 years ago, with no or only minor loss of whole-genome heterozygosity. In fact, most of the changes at coding genes and linked regions that differentiate wild and domestic genomes are already present in the ancient genomes analysed here, and all ancient domestic genomes dated between 600 and 2,500 years ago are highly variable (at least as variable as modern genomes from the wild). Single seeds from modern cultivars show reduced variation when compared with ancient seeds, indicating that intensive selection within cultivars in the past few centuries probably partitioned ancestral variation within different genetically homogenous cultivars. When cultivars from different Andean regions are pooled, the genomic variation of the pool is higher than that observed in the pool of ancient seeds from north and central western Argentina. Considering that most desirable phenotypic traits are probably controlled by multiple polymorphic genes10, a plausible explanation of this decoupling of selection and genetic erosion is that early farmers applied a relatively weak selection pressure2 by using many phenotypically similar but genetically diverse individuals as parents. Our results imply that selection strategies during the past few centuries, as compared with earlier times, more intensively reduced genetic variation within cultivars and produced further improvements by focusing on a few plants carrying the traits of interest, at the cost of marked genetic erosion within Andean landraces.
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Productos Agrícolas/genética , Productos Agrícolas/historia , Domesticación , Agricultores/psicología , Genoma de Planta , Phaseolus/genética , Argentina , Agricultores/estadística & datos numéricos , Variación Genética , Genotipo , Historia AntiguaRESUMEN
In this paper, diagnostic analyses on 12th-13th century byzantine wall paintings in the abbey of Santa Maria di Cerrate (Southern Italy) were carried out preparatory to restoration work promoted by FAI (Fondo Ambiente Italiano). Both the pigments and the areas with a bleaching alteration in the frescoes were analysed using the energy dispersive X-ray fluorescence, the Raman spectroscopy and the scanning electron microscopy equipped with energy dispersive X-ray spectrometer. In particular, ED-XRF analyses were performed in situ on wall paintings, while Raman spectroscopy and SEM-EDX were accomplished on frescoes fragments. The results obtained allowed to determine the chemical composition of the pigments, helping to identify the original parts of the frescoes from the retouched ones. The work also provided important information about the historical-artistic context of the abbey. Archaeometric investigations have indeed revealed the pictorial technique used for the wall paintings of the abbey, as well as the presence of some very valuable pigments, such as lapis lazuli blue.
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The determination of the age of elephant ivory is a crucial aspect in the fight against illegal ivory trade which is still a relevant problem having triggered the decline of elephant populations due to poaching in different areas of the globe. Indeed, the absolute dating of the ivory allows, in forensics practice, to establish whether a determined sample or object was obtained and imported illegally, violating the international trade ban. In this frame the use radiocarbon dating has surely a great potential and is widely used. In this paper we review the potential of the method in this field, highlighting its advantages and drawbacks. In particular we show, through the discussion of real cases, how it is possible to improve the achievable chronological resolution by refining the obtained ages trough the proper use of available information and considerations.
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OBJECTIVE: To evaluate, via a multidisciplinary approach, a distinctive paleopathological condition believed to be fibrous dysplasia, found on a 19th/20th century skeleton from Certosa Monumental Cemetery, Bologna, Italy. MATERIALS: A skeletonized cranium and mandible recovered from an ossuary in 2014. METHODS: Pathological alterations were analysed by radiological examination, dental macrowear, histopathological and genetic analyses. RESULT: The skeleton is believed to be an adult male. Differential diagnoses include Paget's disease, McCune-Albright syndrome, osteochondroma and osteosarcoma. The radiographic findings, along with the solitary nature of the lesions, are strong evidence for the diagnosis of fibrous dysplasia (FD). Genetic analysis further revealed a frequency of Ë1% of mutant alleles with the R201C substitution, one of the post-zygotic activating mutation frequently associated with FD. CONCLUSIONS: The multi-analytical method employed suggests a diagnosis of monostotic form of FD. The diagnostic design incorporates multiple lines of evidence, including macroscopic, histopathological, and genetic analyses. SIGNIFICANCE: Through the use of a multi-analytic approach, robust diagnoses can be offered. This case serves as one of the oldest examples of FD from an historical context. The genetic mutation detected, associated with FD, has not been previously reported in historical/ancient samples.
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Displasia Fibrosa Craneofacial/diagnóstico por imagen , Adulto , Sustitución de Aminoácidos , Cementerios/historia , Displasia Fibrosa Craneofacial/genética , Displasia Fibrosa Craneofacial/historia , Displasia Fibrosa Craneofacial/patología , Displasia Fibrosa Poliostótica/diagnóstico por imagen , Displasia Fibrosa Poliostótica/historia , Displasia Fibrosa Poliostótica/patología , Historia del Siglo XIX , Historia del Siglo XX , Humanos , Italia , Masculino , Mutación , Osteítis Deformante/diagnóstico por imagen , Osteítis Deformante/patología , Osteosarcoma/diagnóstico por imagen , Osteosarcoma/historia , Osteosarcoma/patología , Tomografía Computarizada por Rayos X/historiaRESUMEN
In the process of dating the oldest trees, which are often hollow, we developed a new method that combines tree-ring cross dating and wiggle matching radiocarbon techniques on wood samples extracted from the stem and from exposed roots. The method can be illustrated by the following steps: â¢crossdated tree-ring series from trunk cores reveal a multi-century tree age, and the hollow section is large enough to contain several more years (decades to centuries)â¢exposed roots can be cored for acquiring wood samples older than the stem cores and for construction of a floating root average tree-ring seriesâ¢if synchronization between stem and exposed roots is unclear, proceed to date the root wood samples by radiocarbon wiggle matching; match root and stem tree-ring series within the radiocarbon-dated period to more accurately date the tree. This new multistep dating method allowed for refining the age estimation of the oldest Pinus heldreichii tree in Pollino National Park by 166 years, to 789 CE. This tree, which we named Italus, was 1229 years old in 2017, making it the oldest, scientifically dated, living tree in Europe. Any study that relies on tree age determination for paleo-reconstructions, for biological and genetic research on what controls longevity, or for understanding structural dynamics and succession in old-growth forests, would potentially benefit from the multistep dating method we tested.
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Giovanni Pico della Mirandola and Angelo Poliziano were two of the most important humanists of the Italian Renaissance. They died suddenly in 1494 and their deaths have been for centuries a subject of debate. The exhumation of their remains offered the opportunity to study the cause of their death through a multidisciplinary research project. Anthropological analyses, together with documentary evidences, radiocarbon dating and ancient DNA analysis supported the identification of the remains attributed to Pico. Macroscopic examination did not reveal paleopathological lesions or signs related to syphilis. Heavy metals analysis, carried out on bones and mummified tissues, showed that in Pico's remains there were potentially lethal levels of arsenic, supporting the philosopher's poisoning theory reported by documentary sources. The arsenic concentrations obtained from analysis of Poliziano's remains, are probably more related to an As chronic exposure or diagenetic processes rather than poisoning.
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Intoxicación por Arsénico/historia , Arsénico/análisis , Huesos/química , Radioisótopos de Carbono , ADN Antiguo , Exposición a Riesgos Ambientales/efectos adversos , Toxicología Forense , Historia del Siglo XV , Humanos , Italia , Masculino , Microscopía , Microscopía Electrónica de Rastreo , Momias , Análisis Espectral/métodosRESUMEN
Mixed iron-manganese oxide nanoparticles, synthesized by a simple procedure, were used to remove nickel ion from aqueous solutions. Nanostructures, prepared by using different weight percents of manganese, were characterized by transmission electron microscopy, selected area diffraction, X-ray diffraction, Raman spectroscopy, and vibrating sample magnetometry. Adsorption/desorption isotherm curves demonstrated that manganese inclusions enhance the specific surface area three times and the pores volume ten times. This feature was crucial to decontaminate both aqueous samples and food extracts from nickel ion. Efficient removal of Ni2+ was highlighted by the well-known dimethylglyoxime test and by ICP-MS analysis and the possibility of regenerating the nanostructure was obtained by a washing treatment in disodium ethylenediaminetetraacetate solution.
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This paper concerns the changes occurred over both decennial and millennial spans of time in a sponge assemblage present in coralligenous biogenic build-ups growing at 15 m depth in the Ligurian Sea (Western Mediterranean). The comparison of the sponge diversity after a time interval of about 40 years (1973-2014) showed a significant reduction in species richness (about 45%). This decrease affected mainly the massive/erect sponges, and in particular the subclass Keratosa, with a species loss of 67%, while the encrusting and cavity dwelling sponges lost the 36% and 50%, respectively. The boring sponges lost only one species (25%). This changing pattern suggested that the inner habitat of the bioconstructions was less affected by the variations of the environmental conditions or by the human pressures which, on the contrary, strongly affected the species living on the surface of the biogenic build-ups. Five cores extracted from the bioherms, dating back to 3500 YBP, allowed to analyse the siliceous spicules remained trapped in them in order to obtain taxonomic information. Changes at generic level in diversity and abundance were observed at 500/250-years intervals, ranging between 19 and 33 genera. The number of genera showed a sharp decrease since 3500-3000 to 3000-2500 YBP. After this period, the genera regularly increased until 1500-1250 YBP, from when they progressively decreased until 1000-500 YBP. Tentatively, these changes could be related to the different climatic periods that followed one another in the Mediterranean area within the considered time span. The recent depletion in sponge richness recorded in the Ligurian coralligenous can be considered relevant. In fact, the analysis of the spicules indicated that the sponges living in these coralligenous habitats remained enough stable during 3000 years, but could have lost a significant part of their biodiversity in the last decades, coinciding with a series of warming episodes.