RESUMEN
BACKGROUND: Although the COVID-19 pandemic has increased the prevalence of cases with olfactory loss, other respiratory viruses can also cause this condition. We aimed to compare the prevalence of acute SARS-CoV-2 infection and other respiratory viruses in patients with sudden smell loss, and to assess the impact of SARS-CoV-2 viral load and co-infection on olfactory symptoms. METHODS: Patients with sudden smell loss were recruited in a multicenter prospective cohort study in 15 hospitals in Brazil. Clinical questionnaire, Connecticut Chemosensory Clinical Research Center (CCCRC) olfactory test and nasopharyngeal swab to perform a PCR-based respiratory viral panel were collected at first visit (day 0) and 30 and 60 days after recruitment. RESULTS: 188 of 213 patients presented positive test result for SARS-CoV-2, among which 65 were co-infected with other respiratory viruses (e.g., rhinovirus, enterovirus, and parainfluenza). 25 had negative test results for SARS-CoV-2. Patients in both SARSCoV-2 and non-SARS-CoV-2 groups had objective anosmia (less than 2 points according to the psychophysical olfactory CCCRC) at day 0, with no significant difference between them. Both groups had significant smell scores improvement after 30 and 60 days, with no difference between them. Co-infection with other respiratory viruses, and SARS-CoV-2 viral load did not impact olfactory scores. CONCLUSION: Patients with sudden smell loss associated with SARS-CoV-2 and other respiratory viruses had similar presentation, with most participants initiating with anosmia, and total or near total recovery after 60 days. SARS-CoV-2 viral load and co-infections with other respiratory viruses were not associated with poorer olfactory outcomes.
Asunto(s)
COVID-19 , Coinfección , Trastornos del Olfato , Humanos , SARS-CoV-2 , COVID-19/complicaciones , Anosmia/complicaciones , Anosmia/epidemiología , Estudios Prospectivos , Pandemias , Coinfección/complicaciones , Coinfección/epidemiología , Trastornos del Olfato/diagnóstico , Trastornos del Olfato/epidemiología , Trastornos del Olfato/etiología , OlfatoRESUMEN
AIM: To determine feasibility, reliability and validity of the shortened version of the Quality of Life in Epilepsy Inventory-10 (QOLIE-10) in a population of refractory epilepsy adult patients in the Colombian Caribbean. PATIENTS AND METHODS: Data were collected from 63 adult refractory epilepsy patients. The ten items of QOLIE-10 were derived from the Spanish version of QOLIE-89. We assess feasibility, validity, factorial analysis with communalities, reliability through internal consistency and sensitivity to change. RESULTS: Clinical and demographic features were determined; quality of life was established through frequencies. Construct validity: through factor analysis communalities there were no items considered irrelevant and were grouped into one single dimension. Kaiser-Meyer-Olkin: 0.891. Bartlett's test of sphericity: p < 0.001. Average intraclass correlation coefficient 0.843. The internal consistency reliability coefficient (Cronbach's alpha) was 0.98. The QOLIE-10 questionnaire for the assessment of health-related quality of life was validated in Spanish for adult patients with refractory epilepsy with excellent validity, reliability parameters and easy, quick filling. CONCLUSIONS: The QOLIE-10 questionnaire is a valid and reliable tool for use in adult patients with refractory epilepsy in Colombia. Health professionals are encouraged to use this questionnaire to routinely examine the influences of the disease process in epilepsy patients.
TITLE: Validez y fiabilidad del instrumento para evaluación de calidad de vida relacionada con la salud en epilepsia QOLIE-10 en pacientes adultos con epilepsia refractaria en un centro neurológico colombiano.Objetivo. Determinar la factibilidad, la fiabilidad y la validez de la versión en castellano del cuestionario breve de calidad de vida relacionada con la salud en epilepsia QOLIE-10 en una población de pacientes adultos con epilepsia refractaria de un centro de referencia de enfermedades neurológicas del Caribe colombiano. Pacientes y métodos. Se recogieron datos de 63 pacientes con epilepsia refractaria. Los 10 ítems del cuestionario QOLIE-10 se derivaron de la versión en castellano del cuestionario QOLIE-89. Se evaluó la factibilidad, la validez, el análisis factorial con comunalidades, la fiabilidad a través de la consistencia interna y la sensibilidad al cambio. Resultados. Se determinaron las características demográficas y clínicas, y se determinó la calidad de vida por medio de las frecuencias. Validez de constructo: análisis factorial y comunalidades, no se encontraron ítems no relevantes y se agruparon en una sola dimensión. Kaiser-Meyer-Olkin: 0,891. Esfericidad de Barlett: p menos de 0,001. Coeficiente de correlación intraclase media: 0,843. Fiabilidad: consistencia interna alfa de Cronbach, 0,98. Se validó el cuestionario QOLIE-10 de calidad de vida relacionada con la salud en adultos con epilepsia refractaria en castellano, con excelentes parámetros de fiabilidad y validez, y una administración rápida y fácil. Conclusiones. El cuestionario QOLIE-10 se considera una herramienta válida y fiable para su uso en la población de pacientes con epilepsia refractaria en Colombia. Se alienta a los profesionales de la salud a usar este cuestionario para examinar de manera rutinaria la influencia del proceso de enfermedad en la calidad de vida de los pacientes con epilepsia.
Asunto(s)
Autoevaluación Diagnóstica , Epilepsia Refractaria , Calidad de Vida , Adulto , Colombia , Estudios de Factibilidad , Femenino , Instituciones de Salud , Humanos , Masculino , Reproducibilidad de los Resultados , Adulto JovenRESUMEN
In the present experiments we investigated a possible involvement of imidazoline receptors of the paraventricular nucleus (PVN) of the hypothalamus on the pressor effects of the angiotensin II (ANG II) injected into the subfornical organ (SFO), in male Holtzman rats (250-300 g) with a cannula implanted into the third ventricle (3rdV), PVN and SFO. At first we tested the participation of alpha 2 and imidazoline agonist and antagonist compounds on the pressor effect of ANG II injected into the 3rdV. Based on the results we may conclude that clonidine associated with rilmenidine was able to block the hypertensive response to ANG II. The ANG II (20 pmol) injected into SFO induced a robust increase in blood pressure (37 +/- 2 mmHg). Isotonic saline (0.15 M) NaCl did not produce any change in blood pressure (5 +/- 2 mmHg). The injection of rilmenidine (30 micrograms/kg/1 microL), an imidazoline agonist agent injected into PVN before ANG II injection into SFO, blocked the pressor effect of ANG II (5 +/- 2 mmHg). Also, the injection of idazoxan (60 micrograms/kg/microL) before rilmenidine blocked the inhibitory effect of rilmenidine on blood pressure (39 +/- 4 mmHg). The injection of clonidine (20 nmol/microL) prior to ANG II into the 3rdV produced a decreased in arterial blood pressure (37 +/- 2 mmHg) to (15 +/- 4 mmHg). The injection of yohimbine (80 nmol/microL) prior to clonidine blocked the effect of clonidine on the effect of ANG II (27 +/- 2 mmHg). The injection of rilmenidine prior to ANG II also induced a decrease in arterial blood pressure (10 +/- 3 mmHg). The injection of idazoxan prior to rilmenidine also blocked the inhibitory effect of rilmenidine (24 +/- 3 mmHg). In summary, the present study demonstrated that rilmenidine decreases the hypertensive effect of ANG II, with more potency than clonidine, even when injected into 3rdV or PVN. This study established that the PVN interacts with SFO by imidazoline receptors in order to control the arterial blood pressure.
Asunto(s)
Agonistas alfa-Adrenérgicos/farmacología , Angiotensina II/farmacología , Presión Sanguínea/fisiología , Núcleo Hipotalámico Paraventricular/fisiología , Receptores de Droga/fisiología , Órgano Subfornical/efectos de los fármacos , Animales , Presión Sanguínea/efectos de los fármacos , Receptores de Imidazolina , Inyecciones Intraventriculares , Masculino , Ratas , Ratas Sprague-Dawley , Estimulación QuímicaRESUMEN
We investigated the influence of ibotenic acid lesions of the medial hypothalamus (MH) on salt appetite and arterial blood pressure responses induced by angiotensinergic and adrenergic stimulation of the median preoptic nucleus (MnPO) of rats. Previous injection of the adrenergic agonists norepinephrine, clonidine, phenylephrine, and isoproterenol into the MnPO of sham MH-lesioned rats caused no change in the sodium intake induced by ANG II. ANG II injected into the MnPO of MH-lesioned rats increased sodium intake compared with sham-lesioned rats. Previous injection of clonidine and isoproterenol increased, whereas phenylephrine abolished the salt intake induced by ANG II into the MnPO of MH-lesioned rats. Previous injection of norepinephrine and clonidine into the MnPO of sham MH-lesioned rats caused no change in the mean arterial pressure (MAP) induced by ANG II. Under the same conditions, previous injection of phenylephrine increased, whereas isoproterenol reversed the increase in MAP induced by angiotensin II (ANG II). ANG II injected into the MnPO of MH-lesioned rats induce a decrease in MAP compared with sham-lesioned rats. Previous injection of phenylephrine or norepinephrine into the MnPO of MH-lesioned rats induced a negative MAP, whereas pretreatment with clonidine or isoproterenol increased the MAP produced by ANG II injected into the MnPO of sham- or MH-lesioned rats. These data show that ibotenic acid lesion of the MH increases the sodium intake and pressor responses induced by the concomitant angiotensinergic, alpha 2 and beta adrenergic activation of the MnPO, whereas alpha 1 activation may have opposite effects. MH involvement in excitatory and inhibitory mechanisms related to sodium intake and MAP control is suggested.
Asunto(s)
Presión Sanguínea/efectos de los fármacos , Ingestión de Alimentos/fisiología , Agonistas de Aminoácidos Excitadores/toxicidad , Hipotálamo Medio/fisiología , Ácido Iboténico/toxicidad , Área Preóptica/fisiología , Sodio en la Dieta , Agonistas Adrenérgicos/farmacología , Angiotensina II/farmacología , Animales , Agonistas de Aminoácidos Excitadores/administración & dosificación , Hipotálamo Medio/anatomía & histología , Hipotálamo Medio/efectos de los fármacos , Ácido Iboténico/administración & dosificación , Inyecciones , Masculino , Área Preóptica/anatomía & histología , Área Preóptica/efectos de los fármacos , RatasRESUMEN
We determined the effects of losartan and CGP42112A (selective ligands of the AT1 and AT2 angiotensin receptors, respectively) and salarasin (a relatively nonselective angiotensin receptor antagonist) on urinary volume and urinary sodium and potassium excretion induced by administration of angiotensin II (ANG II) into the paraventricular nucleus (PVN) of conscious rats. Both the AT1 and AT2 ligands and salarasin administered in the presence of ANG II elicited a concentration-dependent inhibition of urine excretion, but losartan inhibited only 75% of this response. The IC50 for salarasin, CGP42112A, and losartan was 0.01, 0.05, and 6 nM, respectively. Previous treatment with saralasin, CGP42112A and losartan competitively antagonized the natriuretic responses to PVN administration of ANG II, and the IC50 values were 0.09, 0.48, and 10 nM, respectively. The maximum response to losartan was 65% of that obtained with saralasin. Pretreatment with saralasin, losartan, and CGP42112A injected into the PVN caused shifts to the right of the concentration-response curves, but the losartan concentrations were disproportionately greater compared with salarasin or CGP42112A. The IC50 values were 0.06, 0.5, and 7.0 for salarasin, CGP42112A, and losartan, respectively. These results suggest that both AT1 and AT2 receptor subtypes in the PVN are involved in ANG II-related urine, sodium, and potassium excretion, and that the inhibitory responses to AT2 blockade are predominant.
Asunto(s)
Angiotensina II/metabolismo , Riñón/efectos de los fármacos , Núcleo Hipotalámico Paraventricular , Receptores de Angiotensina/metabolismo , Animales , Inyecciones Intraventriculares , Ligandos , Losartán/administración & dosificación , Losartán/metabolismo , Losartán/farmacología , Masculino , Oligopéptidos/administración & dosificación , Oligopéptidos/metabolismo , Oligopéptidos/farmacología , Potasio/orina , Ratas , Ratas Sprague-Dawley , Saralasina/administración & dosificación , Saralasina/metabolismo , Saralasina/farmacología , Sodio/orinaRESUMEN
The specific arginine(8)-vasopressin (AVP) V(1) receptors antagonist (AAVP) was injected (20, 40 and 80 nmol) into the lateral septal area (LSA) to determine the effects of selective septal V(1) receptor on water and 3% sodium intake in rats. Was also observed the effects of losartan and CGP42112A (select ligands of the AT(1) and AT(2) ANG II receptors, respectively) injected into LSA prior AVP on the same appetites. Twenty-four hours before the experiments, the rats were deprived of water. The volume of drug solution injected was 0.5 microl. Water and sodium intake were measured at 0.25, 0.5, 1.0 and 2.0 h. Injection of AVP reduced the water and sodium ingestion vs. control (0.15 M saline). Pre-treatment with AAVP (40, 80 and 160 nmol) did not alter the decrease in the water ingestion induced by AVP, whereas AAVP abolished the action of AVP-induced sodium intake. Losartan (40, 80 and 160 nmol) did not alter the effect of AVP on water and sodium intake, whereas CGP42112A (20, 40 and 60 nmol) at the first 30 min increased water ingestion. Losartan and CGP42112A together increased the actions of AVP, showing more pronounced effects than when the two antagonists were injected alone. The results showed that AVP inhibited the appetites and these effects were increased by the AAVP. The involvement of angiotensinergic receptors in the effects of AVP is also suggested.
Asunto(s)
Arginina Vasopresina/análogos & derivados , Arginina Vasopresina/farmacología , Ingestión de Líquidos/fisiología , Receptores de Angiotensina/fisiología , Receptores de Vasopresinas/fisiología , Cloruro de Sodio Dietético/administración & dosificación , Bloqueadores del Receptor Tipo 1 de Angiotensina II , Bloqueadores del Receptor Tipo 2 de Angiotensina II , Antagonistas de Receptores de Angiotensina , Animales , Antagonistas de los Receptores de Hormonas Antidiuréticas , Arginina Vasopresina/antagonistas & inhibidores , Relación Dosis-Respuesta a Droga , Ingestión de Líquidos/efectos de los fármacos , Histocitoquímica , Losartán/farmacología , Masculino , Oligopéptidos/farmacología , Ratas , Ratas Sprague-Dawley , Receptor de Angiotensina Tipo 1/fisiología , Receptor de Angiotensina Tipo 2/fisiología , Tabique del Cerebro/efectos de los fármacos , Tabique del Cerebro/fisiología , Sodio/metabolismo , Cloruro de Sodio Dietético/metabolismo , Equilibrio Hidroelectrolítico/efectos de los fármacosRESUMEN
The present experiments were conducted to investigate the role of the alpha(1A)-, alpha(1B), beta(1)- and beta(2)-adrenoceptors of the lateral hypothalamus (LH) on the water and salt intake responses elicited by subfornical organ (SFO) injection of angiotensin II (ANG II) in rats. 5-methylurapidil (an alpha(1A)-adrenergic antagonist), cyclazosin (an alpha(1B)-adrenergic antagonist) and ICI-118,551 (a beta(2)-adrenergic antagonist) injected into the LH produced a dose-dependent reduction, whereas efaroxan (an alpha(2)-antagonist) increased the water intake induced by administration of ANG II into the SFO. These data show that injection of 5-methylurapidil into the LH prior to ANG II into the SFO increased the water and sodium intake induced by the injection of ANG II. The present data also show that atenolol (a beta(1)-adrenergic antagonist), ICI-118,551, cyclazosin, or efaroxan injected into the LH reduced in a dose-dependent manner the water and sodium intake to angiotensinergic activation of SFO. Thus, the alpha(1)- and beta-adrenoceptors of the LH are possibly involved with central mechanisms dependent on ANG II and SFO that control water and sodium intake.
Asunto(s)
Angiotensina II/farmacología , Ingestión de Líquidos/efectos de los fármacos , Área Hipotalámica Lateral/efectos de los fármacos , Receptores Adrenérgicos alfa/efectos de los fármacos , Receptores Adrenérgicos beta/efectos de los fármacos , Cloruro de Sodio Dietético , Vasoconstrictores/farmacología , Antagonistas Adrenérgicos alfa/farmacología , Antagonistas Adrenérgicos beta/farmacología , Animales , Ingestión de Líquidos/fisiología , Área Hipotalámica Lateral/fisiología , Masculino , Ratas , Ratas Sprague-Dawley , Receptores Adrenérgicos alfa/fisiología , Receptores Adrenérgicos beta/fisiología , Órgano Subfornical/efectos de los fármacos , Órgano Subfornical/fisiologíaRESUMEN
In this study, we investigated the participation of adrenergic neurotransmission in angiotensin II- (ANGII)-induced water intake and urinary electrolyte excretion by means of injection of the alpha 1-, alpha 2-, and beta-adrenoceptor antagonists and ANGII into the medial preoptic area (MPOA) in rats. Prazosin (an alpha 1-adrenergic antagonist) antagonized the water ingestion, Na+, K+ and urine excretion induced by ANGII, whereas yohimbine (an alpha 2-adrenergic antagonist) enhanced the Na+, K+ and urine excretion induced by ANGII. Propranolol (a nonselective beta-adrenoceptor blocker) antagonized the water ingestion and enhanced the Na+, and urine excretion induced by ANGII. Previous treatment with prazosin reduced the pressor responses to ANGII, whereas yohimbine had opposite effects. Previous injection of propranolol produced no effects in the pressor responses to ANGII. These results suggest that the adrenergic neurotransmission in the MPOA may actively participate in ANGII-induced dipsogenesis, natriuresis, kaliuresis and diuresis in a process that involves alpha 1-, alpha 2-, and beta-adrenoceptors.
Asunto(s)
Angiotensina II/farmacología , Vías Autónomas/fisiología , Ingestión de Líquidos/efectos de los fármacos , Riñón/metabolismo , Área Preóptica/fisiología , Antagonistas de Receptores Adrenérgicos alfa 1 , Antagonistas de Receptores Adrenérgicos alfa 2 , Antagonistas Adrenérgicos beta/farmacología , Animales , Vías Autónomas/anatomía & histología , Presión Sanguínea/efectos de los fármacos , Inyecciones , Riñón/efectos de los fármacos , Masculino , Natriuresis/efectos de los fármacos , Potasio/orina , Prazosina/administración & dosificación , Prazosina/farmacología , Área Preóptica/anatomía & histología , Propranolol/administración & dosificación , Propranolol/farmacología , Ratas , Yohimbina/administración & dosificación , Yohimbina/farmacologíaRESUMEN
It has been demonstrated that peripheral or intracerebroventricular (i.c.v.) administration of the alpha 2-adrenoceptor agonist, clonidine, blocks the water intake induced by several dipsogenic stimuli in rats. In the present investigation we studied the effect of the injection of clonidine, phenylephrine, prazosin or yohimbine into the lateral hypothalamic area (LHA) on the water intake induced by water deprivation or central angiotensin II (AII) in rats. Rats with chronic cannulas implanted into the lateral ventricle and LHA were used. Injection of clonidine or phenylephrine into the LHA reduced the water intake produced by both water deprivation and i.c.v. injection of AII. Previous injection of the alpha 1- or alpha 2-adrenoceptor antagonists, prazosin or yohimbine, into the LHA reduced the antidipsogenic effect of clonidine or phenylephrine injected into the same area. These results suggest that the alpha 1- and alpha 2-adrenergic receptors of the hypothalamus are part of the central inhibitory system for the thirst produced by dehydration or central AII.
Asunto(s)
Clonidina/farmacología , Ingestión de Líquidos/efectos de los fármacos , Área Hipotalámica Lateral/efectos de los fármacos , Fenilefrina/farmacología , Angiotensina II/administración & dosificación , Angiotensina II/farmacología , Animales , Clonidina/administración & dosificación , Deshidratación/psicología , Ingestión de Alimentos/efectos de los fármacos , Área Hipotalámica Lateral/fisiología , Técnicas In Vitro , Inyecciones , Inyecciones Intraventriculares , Masculino , Fenilefrina/administración & dosificación , Fenilefrina/antagonistas & inhibidores , Prazosina/farmacología , Ratas , Yohimbina/farmacologíaRESUMEN
The effect of intravenous infusion of hypertonic saline (HS, 7.5% NaCl) on the recovery of mean arterial pressure (MAP) during hemorrhage was studied in sham-operated rats and in rats with electrolytic lesion in the anteroventral third ventricle (AV3V) region. After intravenous infusion of 7.5% NaCl (4 ml/kg b.wt.), MAP increased from about 60 to 90 mmHg in sham rats and became stable at this level during all the time of observation (30 min). In AV3V-lesioned rats, after the same infusion, the MAP increased to 80 mmHg, but returned to the pre-infusion levels within 30 min. These results show that the integrity of the AV3V region is important for the beneficial effect of HS during hemorrhagic shock in rats. The AV3V lesion disrupts neural pathways involved in the maintenance of fluid balance and these changes probably abolish the effect of hypertonic saline.
Asunto(s)
Ventrículos Cerebrales/fisiología , Soluciones Hipertónicas/uso terapéutico , Choque Hemorrágico/tratamiento farmacológico , Animales , Presión Sanguínea/efectos de los fármacos , Masculino , Ratas , Choque Hemorrágico/fisiopatologíaRESUMEN
The effect of intravenous infusion of hypertonic saline (HS, 7.5% NaCl) on the recovery of mean arterial pressure (MAP) after hemorrhage was studied in sham-operated rats and in rats with electrolytic lesion of the anteroventral third ventricle (AV3V) region (4 h, 4 and 20 days). Rats anesthetized with thiopental sodium were bled (about 2.8 ml/100 g) until the MAP was stabilized at the level of 60 mmHg for 30 min. In sham-lesioned rats, MAP increased to 90 mmHg and became stable near this level after intravenous infusion of 7.5% NaCl (4 ml/kg b.wt.). In AV3V-lesioned rats, the same infusion induced a smaller increase in MAP (80 mmHg) and the MAP returned to pre-infusion levels within 30 min. These results show that the AV3V region plays an important role in the recovery of arterial pressure induced by hypertonic saline in rats submitted to hemorrhagic shock.
Asunto(s)
Presión Sanguínea/fisiología , Ventrículos Cerebrales/fisiología , Choque Hemorrágico/fisiopatología , Animales , Presión Sanguínea/efectos de los fármacos , Encéfalo/patología , Ventrículos Cerebrales/patología , Hematócrito , Masculino , Miocardio/patología , Potasio/sangre , Ratas , Ratas Endogámicas , Solución Salina Hipertónica , Sodio/sangreRESUMEN
In the present study we investigated the effect of anteroventral third ventricle (AV3V) lesion on pressor, dipsogenic, natriuretic and kaliuretic responses induced by the injection of carbachol (a cholinergic agonist) into the medial septal area (MSA) of rats. Male rats with sham or AV3V lesion and a stainless-steel cannula implanted into the MSA were used. Carbachol (2 nmol) injected into the MSA in sham lesion rats produced pressor (43 +/- 2 mmHg), dipsogenic (9.6 +/- 1.2 ml/h), natriuretic (531 +/- 82 microEq/120 min) and kaliuretic (164 +/- 14 microEq/120 min) responses. In AV3V-lesioned rats (1-5 days and 14-18 days), the pressor (11 +/- 2 and 14 +/- 2 mmHg, respectively), dipsogenic (1.9 +/- 0.7 and 1.4 +/- 0.6 ml/h), natriuretic (21 +/- 5 and 159 +/- 44 microEq/120 min) and kaliuretic (124 +/- 14 and 86 +/- 13 microEq/120 min) responses induced by carbachol injection into the MSA were reduced. These results show that the AV3V region is essential for the pressor, dipsogenic, natriuretic and kaliuretic responses induced by cholinergic activation of the MSA in rats.
Asunto(s)
Presión Sanguínea/efectos de los fármacos , Carbacol/farmacología , Ventrículos Cerebrales/fisiología , Natriuresis/efectos de los fármacos , Receptores Colinérgicos/efectos de los fármacos , Tabique Pelúcido/efectos de los fármacos , Sed/efectos de los fármacos , Animales , Frecuencia Cardíaca/efectos de los fármacos , Masculino , Potasio/orina , Ratas , Ratas EndogámicasRESUMEN
The present experiments were conducted to investigate the role of the alpha 1- and alpha 2-adrenergic receptors of the lateral hypothalamus (LH) on the drinking response elicited by intracerebroventricular (i.c.v.) injections of carbachol and angiotensin II (AII) in rats. Clonidine (an alpha 2-adrenergic agonist) injected into the LH produced a dose-dependent reduction of the drinking responses elicited by i.c.v. administration of carbachol and AII. The alpha 1-adrenergic agonist phenylephrine injected into the LH reduced the dipsogenic response to i.c.v. AII, but not to carbachol. Injection of yohimbine (an alpha 2-adrenergic antagonist) and prazosin (an alpha 1-adrenergic antagonist) into the LH also reduced the water intake produced by i.c.v. injection of AII. Previous injection of alpha 1- or alpha 2-adrenergic antagonists into the LH increased the antidipsogenic effect of clonidine or phenylephrine injected into the same area on the water intake induced by i.c.v. AII. These results show that the alpha 1- and alpha 2-adrenergic receptors of the LH are involved in the control of drinking responses elicited by i.c.v. injection of AII in rats. They also show that clonidine, but not phenylephrine, suppresses the drinking induced by i.c.v. carbachol. The data suggest that the discharge of central alpha-adrenergic receptors has a dual (inhibitory and excitatory) effect on water intake induced by central AII.
Asunto(s)
Angiotensina II/farmacología , Encéfalo/fisiología , Ingestión de Líquidos/efectos de los fármacos , Área Hipotalámica Lateral/metabolismo , Receptores Adrenérgicos alfa/fisiología , Agonistas alfa-Adrenérgicos/farmacología , Antagonistas Adrenérgicos alfa/farmacología , Animales , Carbacol/farmacología , Ingestión de Líquidos/fisiología , Inyecciones Intraventriculares , Masculino , Ratas , Ratas Endogámicas , Privación de Agua/fisiologíaRESUMEN
The present experiments were conducted to investigate the role of the alpha 1-, alpha 2- and beta-adrenergic receptors of the median preoptic area (MnPO) on the water intake and urinary electrolyte excretion, elicited by central injections of angiotensin II (ANG II). Prazosin (an alpha 1-adrenergic receptor antagonist) and yohimbine (an alpha 2-adrenergic receptor antagonist) antagonized the water ingestion, Na+, K+, and urine excretion induced by ANG II. Administration of propranolol, a beta-adrenergic receptor antagonist increased the Na+, K+, and urine excretion induced by ANG II. Previous treatment with prazosin and yohimbine reduced the pressor responses to ANG II. These results suggest that the adrenergic neurotransmission in the MnPO may actively participate in ANG II-induced dipsogenesis, natriuresis, kaliuresis, diuresis and pressor responses in a process that involves alpha 1-, alpha 2-, and beta-adrenoceptors.
Asunto(s)
Angiotensina II/fisiología , Ingestión de Líquidos/fisiología , Riñón/fisiología , Área Preóptica/química , Receptores Adrenérgicos/fisiología , Antagonistas de Receptores Adrenérgicos alfa 1 , Antagonistas de Receptores Adrenérgicos alfa 2 , Antagonistas Adrenérgicos alfa/farmacología , Antagonistas Adrenérgicos beta/farmacología , Animales , Presión Sanguínea/efectos de los fármacos , Presión Sanguínea/fisiología , Ingestión de Líquidos/efectos de los fármacos , Masculino , Potasio/orina , Prazosina/farmacología , Propranolol/farmacología , Ratas , Ratas Sprague-Dawley , Receptores Adrenérgicos alfa 1/fisiología , Receptores Adrenérgicos alfa 2/fisiología , Receptores Adrenérgicos beta/fisiología , Sodio/orina , Micción/efectos de los fármacos , Yohimbina/farmacologíaRESUMEN
To determine whether central alpha 1 and alpha 2-adrenergic mechanisms are involved in urinary sodium and potassium excretion and urine volume induced by angiotensin II (ANGII), these renal parameters were measured in volume-expanded Holtzman rats with cannulas implanted into lateral ventricle (LV) and lateral hypothalamus (LH). The injection of ANGII into LV in rats with volume expansion reduced the sodium, potassium and urine excretion in comparison to the control injections of isotonic saline, whereas prazosin (alpha 1 antagonist) potentiated these effects. Clonidine (alpha 2 agonist) and yohimbine (alpha 2 antagonist) injected into LH previous to injection of ANGII into LV also abolished the inhibitory effect of ANGII. These results suggest that the discharge of central alpha-adrenergic receptors has dual inhibitory and excitatory effect on antinatriuretic, antikaliuretic and antidiuretic effect induced by central ANGII in volume-expanded rats.
Asunto(s)
Angiotensina II/farmacología , Clonidina/farmacología , Hipotálamo/efectos de los fármacos , Prazosina/farmacología , Micción/efectos de los fármacos , Animales , Interacciones Farmacológicas , Masculino , Potasio/metabolismo , Ratas , Ratas Endogámicas , Sodio/metabolismo , Yohimbina/farmacologíaRESUMEN
In the present study we investigated the effect of electrolytic lesion of the medial septal area (MSA) on the dipsogenic, natriuretic, kaliuretic and pressor responses elicited by intracerebroventricular (i.c.v.) injection of the cholinergic agonist carbachol. Freely moving rats with sham or MSA lesion (1-7 days and 14-18 days) and a stainless steel cannula implanted into the lateral ventricle were studied. In sham rats, i.c.v. injection of carbachol (7.5 nmol) produced an increase in water intake (10.2 +/- 1.5 ml/h), mean arterial pressure (MAP) (35 +/- 5 mmHg) and urinary Na+ and K+ excretion (551 +/- 83 and 170 +/- 17 muEq/120 min, resp.). The pressor (18 +/- 3 and 14 +/- 4 mmHg, resp.) and natriuretic responses (178 +/- 58 and 172 +/- 38 muEq/120 min) produced by i.c.v. carbachol in acute or chronic MSA-lesioned rats were reduced. No change was observed in urinary K+ excretion and a reduced water intake (5 +/- 1.3 ml/h) was observed only in acute MSA-lesioned rats. These results suggest that the MSA plays an important role for the pressor and natriuretic responses induced by central cholinergic activation in rats. A small influence of this structure on water intake may also be suggested.
Asunto(s)
Presión Sanguínea/efectos de los fármacos , Encéfalo/fisiología , Carbacol/farmacología , Tabique Pelúcido/fisiología , Sed/efectos de los fármacos , Orina/química , Animales , Ingestión de Líquidos/efectos de los fármacos , Masculino , Natriuresis/efectos de los fármacos , Potasio/orina , Ratas , Ratas EndogámicasRESUMEN
In the present study we investigated the effect of anteroventral third ventricle (AV3V) lesion on pressor, dipsogenic and natriuretic responses produced by the intracerebroventricular (i.c.v.) injection of a cholinergic agonist (carbachol). Freely moving rats with AV3V or sham lesion (1-2 days and 9-12 days) and a delay cannula implanted into the lateral ventricle were studied. Changes in mean arterial pressure (MAP, 1 h record), water intake (1 h) and Na+ excretion (2 h) were analysed after i.c.v. injection of carbachol (7.5 nmol). In sham rats, i.c.v. injection of carbachol produced an increase in MAP (35 +/- 2 mmHg), water ingestion (7.7 +/- 1.2 ml/h) and Na+ excretion (626 +/- 42 microEq/120 min). The effects of i.c.v. carbachol injection were reduced 1-2 days after AV3V lesion (delta MAP = 6 +/- 2 mmHg, water ingestion = 0.3 +/- 0.3 ml/h and Na+ excretion = 31 +/- 11 microEq/120 min) and 9-12 days (delta MAP = 11 +/- 3 mmHg, water ingestion = 3.3 +/- 0.9 ml/h and Na+ excretion = 37 +/- 11 microEq/120 min). These results show that the AV3V region is essential for the full development of the pressor, dipsogenic and natriuretic responses produced by central cholinergic receptors.
Asunto(s)
Presión Sanguínea/efectos de los fármacos , Carbacol/farmacología , Ventrículos Cerebrales/fisiología , Fibras Colinérgicas/fisiología , Conducta de Ingestión de Líquido/efectos de los fármacos , Micción/efectos de los fármacos , Animales , Ventrículos Cerebrales/efectos de los fármacos , Fibras Colinérgicas/efectos de los fármacos , Inyecciones Intraventriculares , Masculino , RatasRESUMEN
The present study was performed to investigate the effect of treatment with furosemide on the pressor response induced by intracerebroventricular (i.c.v.) injections of cholinergic (carbachol) and adrenergic (norepinephrine) agonists, angiotensin II (ANGII) and hypertonic saline (HS, 2 M NaCl). The changes induced by furosemide treatment on the pressor response to intravenous (i.v.) norepinephrine, ANGII and arginine vasopressin (AVP) were also studied. Rats with a stainless-steel cannula implanted into the lateral ventricle (LV) were used. Two injections of furosemide (30 mg/kg b.wt. each) were performed 12 and 1 h before the experiments. Treatment with furosemide reduced the pressor response induced by carbachol, norepinephrine and ANGII i.c.v., but no change was observed in the pressor response to i.c.v. 2 M NaCl. The pressor response to i.v. ANGII and norepinephrine, but not AVP, was also reduced after treatment with furosemide. These results show that the treatment with furosemide impairs the pressor responses induced by central or peripheral administration of adrenergic agonist or ANGII, as well as those induced by central cholinergic activation. The results suggest that the treatment with furosemide impairs central and peripheral pressor responses mediated by sympathetic activation and ANGII, but not those produced by AVP.
Asunto(s)
Angiotensina II/antagonistas & inhibidores , Arginina Vasopresina/farmacología , Presión Sanguínea/efectos de los fármacos , Carbacol/antagonistas & inhibidores , Furosemida/farmacología , Norepinefrina/antagonistas & inhibidores , Solución Salina Hipertónica/farmacología , Vasoconstricción/efectos de los fármacos , Animales , Arginina Vasopresina/administración & dosificación , Carbacol/administración & dosificación , Inyecciones Intravenosas , Inyecciones Intraventriculares , Masculino , Norepinefrina/administración & dosificación , Ratas , Solución Salina Hipertónica/administración & dosificaciónRESUMEN
In the present study we investigated the effects of central (i.c.v.) and subcutaneous (s.c.) injections of a 2 micrograms dose of lisinopryl, an inhibitor of angiotensin I(ANGI)-converting enzyme (CE), on water intake. I.c.v. but not s.c. injection of lisinopryl abolished drinking in response to s.c. isoprenaline (100 micrograms/kg) and significantly reduced drinking in response to 24 h water deprivation or s.c. polyethylene glycol (30% w/v, 10 ml/kg). Lisinopryl had no effect on water intake induced by cellular dehydration (s.c. injection of hypertonic saline (2 M NaCl)). These results are consistent with the hypothesis that lisinopryl acts as a CE blocking agent in the brain. The thrist challenge induced by hypotension using isoprenaline acts primarily by generating ANGII systemically and centrally. The other thirst challenges such as cellular dehydration are independent of the ANGII in the brain. This conclusion was made possible by utilizing a new CE blocking agent at a smaller dose than normally used for other ANG I-CE inhibitors.
Asunto(s)
Inhibidores de la Enzima Convertidora de Angiotensina/farmacología , Ventrículos Cerebrales/fisiología , Conducta de Ingestión de Líquido/efectos de los fármacos , Enalapril/análogos & derivados , Animales , Ventrículos Cerebrales/efectos de los fármacos , Enalapril/administración & dosificación , Enalapril/farmacología , Inyecciones Intraventriculares , Inyecciones Subcutáneas , Isoproterenol/administración & dosificación , Isoproterenol/farmacología , Lisinopril , Masculino , Ratas , Valores de Referencia , Solución Salina Hipertónica , Factores de TiempoRESUMEN
The microinjection of carbachol into the medial preoptic area (MPO) of the rat induced natriuresis, kaliuresis and anti-diuresis in a dose-related manner. Atropine blocked all responses to carbachol. Hexamethonium impaired the dose-response effect of carbachol on kaliuresis, but had no effect on natriuresis and enhanced the antidiuretic effect of carbachol. Nicotine alone had no effects, but pre-treatment with nicotine enhanced the responses to carbachol. These data show that activity of the muscarinic receptors of the MPO increases renal electrolyte and reduces water excretion. They also suggest that nicotinic receptors have an inhibitory effect on water excretion. Nicotine could act through mechanisms unrelated to nicotinic receptors to enhance the effect of the carbachol.