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Nat Biotechnol ; 42(1): 132-138, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-37231263

RESUMEN

We present avidity sequencing, a sequencing chemistry that separately optimizes the processes of stepping along a DNA template and that of identifying each nucleotide within the template. Nucleotide identification uses multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer-nucleotide complexes bound to clonal copies of DNA targets. These polymer-nucleotide substrates, termed avidites, decrease the required concentration of reporting nucleotides from micromolar to nanomolar and yield negligible dissociation rates. Avidity sequencing achieves high accuracy, with 96.2% and 85.4% of base calls having an average of one error per 1,000 and 10,000 base pairs, respectively. We show that the average error rate of avidity sequencing remained stable following a long homopolymer.


Asunto(s)
ADN , Nucleótidos , Nucleótidos/genética , Nucleótidos/química , ADN/genética , ADN/química , Replicación del ADN , Emparejamiento Base , Polímeros
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