Age-related changes in hematological biomarkers in common marmosets.

Researchers and veterinarians often use hematology and clinical chemistry to evaluate animal health. These biomarkers are relatively easy to obtain, and understanding how they change across healthy aging is critical to clinical care and diagnostics for these animals. We aimed to evaluate how clinical biomarkers from a chemistry profile and complete blood count (CBC) change with age in common marmosets (Callithrix jacchus). We assessed blood samples collected during routine physical exams at the Southwest National Primate Research Center and the University of Texas Health San Antonio marmoset colonies from November 2020-November 2021. We found that chemistry and CBC profiles varied based on facility, sex, and age. Significant changes in albumin, phosphorus/creatinine ratio, albumin/globulin ratio, amylase, creatinine, lymphocyte percent, hematocrit, granulocytes percent, lymphocytes, hemoglobin, red cell distribution width, and platelet distribution width were all reported with advancing age. Aged individuals also demonstrated evidence for changes in liver, kidney, and immune system function compared with younger individuals. Our results suggest there may be regular changes associated with healthy aging in marmosets that are outside of the range typically considered as normal values for healthy young individuals, indicating the potential need for redefined healthy ranges for clinical biomarkers in aged animals. Identifying animals that exhibit values outside of this defined healthy aging reference will allow more accurate diagnostics and treatments for aging colonies.

Anoxia hormesis following overwintering diapause boosts bee survivorship and adult performance.

Insect pollination is a crucial component of our ecosystems and biodiversity, but our reliance on this ecosystem service has much broader implications. We depend on these pollination services to produce materials and food. But insect pollinators, especially bees, are in strong decline due to a plethora of factors, least of which are environmental abiotic stressors like climate change. The alfalfa leafcutting bee, Megachile rotundata, is the world's most managed solitary bee and is particularly vulnerable to changes in temperature. This species spends up to ten months overwintering while being exposed to the lowest temperatures of winters and the hottest temperatures of late summer. This results in usage of energy reserves prematurely and asynchronous spring emergence with their food resource. To understand the stress response of these bees and potentially boost their performance, we applied a hormetic framework where bees were exposure to different doses of anoxia (the absence of oxygen) to trigger hormesis; a low-dose stimulatory response known to lower damage and improve performance. We used hormesis on immature bees as a post-winter treatment with the goal of improving springtime performance in adults. One hour of anoxia had no negative effect on adult springtime emergence and bees were quick to recover. These bees were more active than untreated bees, as resistant to starvation, and as long-lived. Higher exposure to anoxia (3 h) was found to be mildly hormetic and 6-h exposures were detrimental. Anoxia hormesis did not represent a significant cost on the energy reserve of overwintering bees but we found that the age at which anoxia is applied will affect the effectiveness of treatment. Our data suggest that anoxia hormesis is a viable intervention to improve springtime performance in overwintering bees.