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Asbestos is a fibrous silicate mineral exhibiting biopersistence and carcinogenic properties and contributes to mesothelioma. Despite the concept of gene-environmental interaction in pathogenesis of mesothelioma, the possible pathophysiological changes of mesothelial cells simultaneously with SET domain containing 2 (SETD2) loss and asbestos exposure remains obscure. Herein, CRISPR/Cas9-mediated SETD2 knockout Met-5A mesothelial cells (Met-5ASETD2-KO ) were established and exposed with crocidolite, an amphibole asbestos. Cell viability of Met-5ASETD2-KO appeared to dramatically decrease with ≥2.5 µg/cm2 crocidolite exposure as compared with Met-5A, although no cytotoxicity and apoptosis changes of Met-5ASETD2-KO and Met-5A was evident with 1.25 µg/cm2 crocidolite exposure for 48 h. RNA sequencing uncovered top 50 differentially expressed genes (DEGs) between 1.25 µg/cm2 crocidolite exposed Met-5ASETD2-KO (Cro-Met-5ASETD2-KO ) and 1.25 µg/cm2 crocidolite exposed Met-5A (Cro-Met-5A), and ITGA4, THBS2, MYL7, RAC2, CADM1, and CLDN11 appeared to be the primary DEGs involved with adhesion in gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Cro-Met-5ASETD2-KO had strong migration but mild adhesion behavior as compared with Cro-Met-5A. Additionally, crocidolite tended to increase migration of Met-5ASETD2-KO but inhibited migration of Met-5A when compared with their corresponding cells without crocidolite exposure, although no further adhesion property changes was evident for both cells in response to crocidolite. Therefore, crocidolite may affect adhesion-related gene expression and modify adhesion and migration behavior for SETD2-depleted Met-5A, which could provide preliminary insight regarding the potential role of SETD2 in the cell behavior of asbestos-related malignant mesothelial cell.
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Amianto , Mesotelioma , Humanos , Asbesto Crocidolita/toxicidad , Asbesto Crocidolita/metabolismo , Epitelio , Amianto/toxicidad , Silicatos , Molécula 1 de Adhesión Celular/metabolismoRESUMEN
PURPOSE: Noninvasive detection of high-risk plaques is still challenging. In this study, we aimed to noninvasively assess αvß3-integrin expression using a customed photoacoustic (PA) computed tomography (PACT)/ultrasound (US) system in atherosclerotic lesions of varying degrees of severity and to explore its potential value for detecting high-risk plaques. METHODS: We constructed αvß3-integrin-targeted ultrasmall gold nanorods (AuNRs) with cyclo Arg-Gly-Asp (cRGD) and tested their properties. Employing C57BL/6 J (wild-type, WT) mice and apolipoprotein E gene knockout (ApoE-/-) mice fed either a chow diet or a high-fat/high-cholesterol diet (HFHCD), we established varying degrees of lesion severity. In vivo PACT/US imaging was performed to assess αvß3-integrin expression in the 4 groups by cRGD-AuNRs. Further histopathologic examination was conducted to evaluate the plaque vulnerability indicators. RESULTS: The data showed that cRGD-AuNRs exhibited excellent photothermal conversion capacity, stability, targeting ability, and biocompatibility. The immunohistochemical results indicated that αvß3-integrin was upregulated with increasing aggravation of the lesions. In vivo PACT/US imaging showed good consistency with αvß3-integrin expression. Notably, ApoE-/- mice fed a HFHCD showed an abrupt PA intensity increase compared with the other groups. The histopathologic examination verified that the atherosclerotic plaques of ApoE-/- mice fed the HFHCD developed unstable phenotypes. Correlation analysis showed that PA intensity was mainly related to inflammation and angiogenesis among all of the indicators. CONCLUSION: Our data indicated that αvß3-integrin is an effective indicator of plaque instability, and noninvasive PACT/US molecular imaging assessment of αvß3-integrin holds promise in detecting high-risk plaques.
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Placa Aterosclerótica , Animales , Ratones , Colesterol/metabolismo , Oro , Integrina alfaVbeta3/metabolismo , Macrófagos/metabolismo , Ratones Endogámicos C57BL , Placa Aterosclerótica/diagnóstico por imagen , Placa Aterosclerótica/metabolismo , Tomografía Computarizada por Rayos X , Ultrasonografía , Ratones Noqueados para ApoERESUMEN
Probiotics have been used to alleviate disease transmission in aquaculture. However, there are limited studies on probiotic use in modulating tilapia lake virus (TiLV). We assessed commercially available probiotic supplements used in TiLV-infected tilapia and performed mortality and cohabitation assays. We developed a mechanistic approach to predict dose-response interactions of probiotic effects on mortality and immune gene response. We used a susceptible-infected-mortality disease model to assess key epidemiological parameters such as transmission rate and basic reproduction number (R0 ) based on our viral load dynamic data. We found that the most marked benefits of probiotics are significantly associated with immune system enhancements (~30%) and reductions in disease transmission (~80%) and R0 (~70%) in tilapia populations, resulting in a higher tolerance of farming densities (~400 fold) in aquaculture. These findings provide early insights as to how probiotic use-related factors may influence TiLV transmission and the immune responses in TiLV-infected tilapia. Our study facilitates understanding the mode of action of probiotics in disease containment and predicting better probiotic dosages in diet and supplements to achieve the optimal culturing conditions. Overall, our analysis assures that further study of rationally designed and targeted probiotics, or mechanistic modelling is warranted on the basis of promising early data of this approach.
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Enfermedades de los Peces , Probióticos , Tilapia , Animales , Acuicultura , Inmunidad , Probióticos/farmacologíaRESUMEN
Nervous necrosis virus (NNV) infection in susceptible grouper larvae has been reported to cause high mortalities, leading to great economic losses in aquaculture industry. Although the effects of NNV vaccines on grouper have been broadly investigated, vaccination strategies have not been fully established. To this end, we introduced the parsimonious epidemiological models that explored the assessment of key epidemiological parameters and how they changed when vaccinations showed the effects. We showed that the models capture the published cumulative mortality data accurately. We estimated a basic reproduction number R0 = 2.44 for NNV transmission in grouper larvae without vaccination. To effectively control NNV transmission by vaccination, a model for disease control was also generalized to attain the goals of controlled reproduction number less than 1. Our results indicated that at least 60% of grouper population needed to be immunized for ~75 min. Our data-driven modelling approach that links the transmission dynamics of NNV and vaccination strategies for grouper has the potential to support evidence-based planning and adaptation of integrated control measures. We encourage that the epidemiology-based framework introduced here can be further implemented for establishing effective vaccination and mitigation actions aimed at controlling diseases in fish farming practices.
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Lubina/virología , Enfermedades de los Peces/prevención & control , Enfermedades de los Peces/virología , Nodaviridae/patogenicidad , Infecciones por Virus ARN/prevención & control , Vacunación/veterinaria , Animales , Acuicultura , Número Básico de Reproducción , Enfermedades de los Peces/transmisión , Larva/virología , Modelos Teóricos , Infecciones por Virus ARN/transmisión , TaiwánRESUMEN
Susceptibility of hard clams Meretrix lusoria to birnavirus (BV) infections caused by temperature variations, from a mechanistic perspective, has rarely been explored. We used a deterministic susceptible-infectious-mortality (SIM) model to derive temperature-dependent key epidemiologic parameters based on data sets of viral infections in hard clams subjected to acute temperature changes. To parameterize seasonal pattern dependence, we estimated monthly based cumulative mortality and basic reproduction numbers (R0 ) between 1997 and 2017 by way of statistical analysis. Two alternative disease control models were also proposed to assess status of controlled temperature-mediated BV infection by using, respectively, control reproduction number (RC )-control line criterion and removal strategy-based control measure. We showed that based on RC -control strategy, when temperatures ranged from 15 to 26.8°C, proportion of susceptible hard clams removed should be at least 0.22%. Based on removal-control strategy, we found that by limiting pond water temperature to 25-30°C, together with increased removal rates and periods to remove hard clams, it is better to remove hard clams from June and August to reduce both mortality rate and spread of BV. Our results can be used to monitor BV transmission potential in hard clams that will contribute to government control strategy to eradicate future BV epidemics.
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Birnaviridae/fisiología , Bivalvos/virología , Frío , Calor , Animales , AcuiculturaRESUMEN
A novel virus, tilapia lake virus (TiLV), has been identified as a key pathogen responsible for disease outbreak and mass mortality of farmed tilapia. We used a deterministic susceptible-infectious-mortality (SIM) model to derive key disease information appraised with published TiLV-induced cumulative mortality data. The relationship between tilapia mortality and TiLV exposure dosages was described by the Hill model. Furthermore, a disease control model was proposed to determine the status of controlled TiLV infection using a parsimonious control reproduction number (RC )-control line criterion. Results showed that the key disease determinants of transmission rate and basic reproduction number (R0 ) could be derived. The median R0 estimate was 2.59 in a cohabitation setting with 2.6 × 105 TCID50 fish-1 TiLV. The present RC -control model can be employed to determine whether TiLV containment is feasible in an outbreak farm by quantifying the current level of transmission. The SIM model can then be applied to predict what additional control is required to manage RC < 1. We offer valuable tools for aquaculture engineers and public health scientists the mechanistic-based assessment that allows a more rigorous evaluation of different control strategies to reduce waterborne diseases in aquaculture farming systems.
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Enfermedades de los Peces/mortalidad , Enfermedades de los Peces/transmisión , Lagos/virología , Infecciones por Orthomyxoviridae/veterinaria , Tilapia/virología , Animales , Acuicultura , Susceptibilidad a Enfermedades , Enfermedades de los Peces/virología , Modelos Teóricos , Infecciones por Orthomyxoviridae/transmisiónRESUMEN
Microplastics (MPs) and nanoplastics (NPs) pollution has emerged as a significant and widespread environmental issue. Humans are inevitably exposed to MPs and NPs via ingestion, inhalation, and dermal contacts from various sources. However, mechanistic knowledge of their distribution, interaction, and potency in the body is still lacking. To address this knowledge gap, we have undertaken the task of elucidating the toxicokinetic (TK) behaviors of MPs and NPs, aiming to provide mechanistic information for constructing a conceptual physiologically based toxicokinetic (PBTK) model to support in silico modeling approaches. Our effort involved a thorough examination of the existing literature and data collation on the presence of MPs in the human body and in vitro/ex vivo/in vivo biodistribution across various cells and tissues. By comprehending the absorption, distribution, metabolism, and excretion mechanisms of MPs and NPs in relation to their physicochemical attributes, we established a foundational understanding of the link between external exposure and internal tissue dosimetry. We observed that particle size and surface chemistry have been thoroughly explored in previous experimental studies. However, certain attributes, such as polymer type, shape, and biofilm/biocorona, warrant attention and further examination. We discussed the fundamental disparities in TK properties of MPs/NPs from those of engineered nanoparticles. We proposed a preliminary PBTK framework with several possible modeling approaches and discussed existing challenges for further investigation. Overall, this article provides a comprehensive compilation of existing TK data of MPs/NPs, a critical overview of TK processes and mechanisms, and proposes potential PBTK modeling approaches, particularly regarding their applicability to the human system, and outlines future perspectives for developing PBTK models and their integration into human health risk assessment of MPs and NPs.
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Microplásticos , Nanopartículas , Toxicocinética , Humanos , Microplásticos/toxicidad , Medición de Riesgo , Nanopartículas/química , Nanopartículas/toxicidad , Exposición a Riesgos Ambientales , Modelos Biológicos , Distribución Tisular , Tamaño de la PartículaRESUMEN
Aeromonas hydrophila has ability to spread tetracycline resistance (tetR) under stresses of oxytetracycline (OTC), one of the most important antibiotics in aquaculture industry. Even though environmental reservoir of Aeromonas allows it to be at interfaces across One Health components, a robust modelling framework for rigorously assessing health risks is currently lacking. We proposed a One Health-based approach and leveraged recent advances in quantitative microbial risk assessment appraised by available dataset to interpret interactions at the human-animal-environment interfaces in various exposure scenarios. The dose-response models were constructed considering the effects on mortality for aquaculture species and tetR genes transfer for humans. A scenario-specific risk assessment on pond species-associated A. hydrophila infection and human gut-associated tetR genes transfer was examined. Risk-based control strategies were involved to test their effectiveness. We showed that farmed shrimp exposed to tetracycline-resistant A. hydrophila in OTC-contaminated water experienced higher infection risk (relative risk: 1.25-1.34). The tetR genes transfer risk for farmers in shrimp ponds (â¼2 × 10-4) and swimmers in coastal areas (â¼4 × 10-6) during autumn exceeded acceptable risk (10-6). This cautionary finding underscores the importance of accounting for monitoring, assessing, and mitigating occupational health hazards among workers in shrimp farming sectors within future One Health-based strategies for managing water infection risks. We recommend that OTC emission rate together with A. hydrophila concentration should be reduced by up to 70-99% to protect human, farmed shrimp, and environmental health. Our predictive framework can be adopted for other systems and be used as a "risk detector" for assessing tetR-related health risks that invoke potential risk management on addressing sustainable mitigation on offsetting residual OTC emission and tetR genes spread in a species-human-environmental health system.
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Aeromonas hydrophila , Acuicultura , Salud Única , Resistencia a la Tetraciclina , Aeromonas hydrophila/efectos de los fármacos , Humanos , Animales , Resistencia a la Tetraciclina/genética , Antibacterianos/farmacología , Medición de Riesgo , Oxitetraciclina/farmacología , Infecciones por Bacterias GramnegativasRESUMEN
Microplastics (MPs) waste is widespread globally in water systems. The opportunistic human pathogen Pseudomonas aeruginosa can cause serious acute and chronic infections that are notoriously difficult to treat. Ciprofloxacin (CIP) is broadly applied as an anti-P. aeruginosa drug. A growing evidence reveals that antibiotic-resistance genes-carrying Pseudomonas aeruginosa were detected on MPs forming plastisphere due to their adsorbability along with high occurrence of CIP in water environments. The MPs-niched CIP-resistant P. aeruginosa has been likely to emerge as an unignorable public health issue. Here, we offered a novel approach to assess the development of CIP-resistant P. aeruginosa under MPs-antibiotic coexistence at a water region scale. By combing the adsorption isotherm models used to estimate CIP condensation around MPs and a pharmacokinetic/pharmacodynamic-based microbial population dynamic model, we predicted the P. aeruginosa development on CIP-adsorbed MPs in waters. Our assessment revealed a high antibiotic resistance in the P. aeruginosa populations (â¼50 %) with a wider range of waterborne total cell counts (â¼10-2-104 cfu mL-1) among water regions in that the resistance proportion was primarily determined by CIP pollution level and relative abundance of various polymer type of MPs. We implicate that water region-specific MPs were highly likely to provide media for P. aeruginosa propagation. Our results highlight the importance of antibiotic-resistant pathogen colonization-emerging environmental medium interactions when addressing global threat from MPs pollution, in the context of MPs-antibiotics co-contamination assessment and for the continued provision of water system management.
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Antibacterianos , Ciprofloxacina , Microplásticos , Pseudomonas aeruginosa , Pseudomonas aeruginosa/efectos de los fármacos , Ciprofloxacina/farmacología , Antibacterianos/farmacología , Contaminantes Químicos del Agua , Farmacorresistencia BacterianaRESUMEN
Crocidolite is a carcinogen contributing to the pathogenesis of malignant mesothelioma. This study aimed to characterize the possible telomere-related events mediating the malignant transformation of mesothelial cells with and without SETD2 under crocidolite exposure. The crocidolite concentration resulting in 90% viable SETD2 knockout Met-5A (Met-5ASETD2-KO) and Met-5A were estimated to be 0.71 µg/cm2 and 1.8 µg/cm2, respectively, during 72 h of exposure, which was further employed in chronical crocidolite exposure during a 72 h exposure interval per time up to 1 month. Chronical crocidolite-exposed Met-5ASETD2-KO (chronical Cro-Met-5ASETD2-KO) had higher colony formation and increased telomerase reverse transcriptase (TERT) protein levels than chronical crocidolite-exposed Met-5A (chronical Cro-Met-5A) and Met-5ASETD2-KO. Chronical Cro-Met-5ASETD2-KO had longer telomere length (TL) than chronical Cro-Met-5A, although there were no changes in TL for either chronical Cro-Met-5A or chronical Cro-Met-5ASETD2-KO compared with their corresponding cells without crocidolite exposure. BIBR 1532, an inhibitor targeting TERT, partially reduced colony formation and TL for chronical Cro-Met-5ASETD2-KO, while BIBR 1532 reduced TL but had no effect on colony formation for chronical Cro-Met-5A. Therefore, SETD2 deficient mesothelial cells are susceptible to malignant transformation during chronical crocidolite exposure, and TERT-dependent TL modification likely partially drives SETD2 loss-mediated early onset of mesothelial malignant transformation.
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Aminobenzoatos , Asbesto Crocidolita , N-Metiltransferasa de Histona-Lisina , Homeostasis del Telómero , Humanos , Aminobenzoatos/metabolismo , Aminobenzoatos/farmacología , Asbesto Crocidolita/toxicidad , Asbesto Crocidolita/metabolismo , Transformación Celular Neoplásica/inducido químicamente , Transformación Celular Neoplásica/metabolismo , Transformación Celular Neoplásica/patología , Epitelio/metabolismo , Epitelio/patología , Naftalenos/metabolismo , Naftalenos/farmacología , N-Metiltransferasa de Histona-Lisina/metabolismoRESUMEN
Despite increasing the public awareness of ubiquity of microplastics (MPs) in air, the issue on particular source of tire wear particles (TWPs) emission into atmosphere and their exposure-associated human health has not received the attention it deserves. Here we linked vehicle kilometers traveled (VKT) estimates covering demography, socio-environmental, and transportation features and emission factors to predict regional emission patterns of TWP-derived atmospheric MPs. A data-driven probabilistic approach was developed to consider variability across the datasets and uncertainty of model parameters in terms of country-level and vehicle-type emissions. We showed that country-specific VKT from billion to trillion vehicle-kilometer resulted in 103-105 metric tons of airborne TWP-derived atmospheric MPs annually in the period 2015-2019, with the highest emissions from passenger cars and heavy-duty vehicles. On average, we found that airborne TWP emissions from passenger cars by country had substantial decreased (up to â¼33%) during COVID-19 lockdowns in 2020 and pronounced increased (by a factor â¼1.9) from vehicle electrification by the next three decades. We conclude that the stunning mass of airborne TWP is a predominant source of atmospheric MP. We underscore the necessity of TWP emissions control among the United States, China, and India. Our findings can be of great use to environmental transportation planners for devising vehicle/tire-oriented decision support tools. Our data offer information to enhance TWP-exposure estimates, to examine long-term exposure trends, and subsequently to improve health risk assessment during pandemic outbreak and future electrification.
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The Wells-Riley model invokes human physiological and engineering parameters to successfully treat airborne transmission of infectious diseases. Applications of this model would have high potentiality on evaluating policy actions and interventions intended to improve public safety efforts on preventing the spread of COVID-19 in an enclosed space. Here, we constructed the interaction relationships among basic reproduction number (R0) - exposure time - indoor population number by using the Wells-Riley model to provide a robust means to assist in planning containment efforts. We quantified SARS-CoV-2 changes in a case study of two Wuhan (Fangcang and Renmin) hospitals. We conducted similar approach to develop control measures in various hospital functional units by taking all accountable factors. We showed that inhalation rates of individuals proved crucial for influencing the transmissibility of SARS-CoV-2, followed by air supply rate and exposure time. We suggest a minimum air change per hour (ACH) of 7 h-1 would be at least appropriate with current room volume requirements in healthcare buildings when indoor population number is < 10 and exposure time is < 1 h with one infector and low activity levels being considered. However, higher ACH (> 16 h-1) with optimal arranged-exposure time/people and high-efficiency air filters would be suggested if more infectors or higher activity levels are presented. Our models lay out a practical metric for evaluating the efficacy of control measures on COVID-19 infection in built environments. Our case studies further indicate that the Wells-Riley model provides a predictive and mechanistic basis for empirical COVID-19 impact reduction planning and gives a framework to treat highly transmissible but mechanically heterogeneous airborne SARS-CoV-2.
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COVID-19 , Humanos , SARS-CoV-2 , HospitalesRESUMEN
This paper presents hardware implementation of an efficient solution to recovering gray-scale data pixels of images that have undergone interpixel interference in holographic data storage systems. The adopted algorithm, called the turbo receiver using interference-aware dual-list (TRIDL) detection, enjoys benefits of low error rate performance and low complexity. To verify the functionality and feasibility, this paper implements TRIDL detection with some circuit design techniques such as resource sharing on a field-programmable gate array.
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Marine microplastics (MPs)-induced threats to shallow-water scleractinian corals are a growing global concern that needs interdisciplinary studies. However, it remains uncertain to what extent the ecotoxicological effects of MPs can explain the potential health impacts on corals at the species-specific scale. Using recent datasets of multiple MPs-induced impacts on coral species, we developed an integrated ecotoxicological modeling approach to quantify the MPs-corals interaction dynamics. Toxicokinetic (TK)-based corals ingestion, egestion, and adhesion processes posed by MPs were comprehensively evaluated. Based on estimated uptake and egestion rates, we showed that corals were much likely to bioaccumulate marine MPs. We applied toxicodynamic (TD) models to appraise time- and concentration-dependent response patterns across MPs-corals systems. We found that marine MPs are highly toxic to corals with a median benchmark concentration causing 10% compromised coral health of 20-40 mg L-1 and a mean growth inhibition rate of ~2% d-1. By providing these key quantitative metrics that may inform scientists to refine existing management strategies to better understand the long-term impact of MPs on coral reef ecosystems. Our TK/TD modeling scheme can help integrating current toxicological findings to encompass a more mechanistic-, ecological-, and process-based understanding of diverse coral ecosystems that are sensitive to MPs stressor varied considerably by species and taxonomic group.
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Antozoos , Microplásticos , Animales , Arrecifes de Coral , Ecosistema , Plásticos , ToxicocinéticaRESUMEN
Microplastics (MPs) dispersed throughout terrestrial ecosystems is a growing global concern, yet their potential risks on terrestrial ecosystems are poorly understood from a mechanistic viewpoint. A novel empirical approach to assess relative sensitivity-risk of terrestrial ecosystems to MPs-posed potential impacts was presented. The risk metrics based on soil-dwelling organism responses-derived biomarkers that could identify whether study regions have sensitivity-risk to MPs were developed. The recently published datasets covering geographic areas worldwide with toxicity endpoints from major soil organism groups (snail, potworm, springtail, nematode, and earthworm) were used to build relationships between species physiological alterations and MPs based on a toxicodynamic-based dose-response model. The regional pollution and species sensitivity distributions combined with a probabilistic risk model were used to compare relative ranks with MPs-associated risks using predicted thresholds. The results found that ecologically sensitive regions with severe responses to soil MPs appeared in areas polluted by sewage sludge and plastic mulching. The results also showed that ~25% of study regions suffered significant MPs-associated ecological risks. This work provides a baseline measurement tool of soil species biomarker-based thresholds for comparing regional sensitivity-risk that highlights why some regions appear to be more sensitive than others and what potential risk impact this has on MPs-polluted terrestrial ecosystems globally.
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Microplásticos , Plásticos , Biomarcadores , Ecosistema , Plásticos/toxicidad , Aguas del Alcantarillado , SueloRESUMEN
A sharp increase in migrant workers has raised concerns for TB epidemics, yet optimal TB control strategies remain unclear in Taiwan regions. This study assessed intervention efforts on reducing tuberculosis (TB) infection among migrant workers. We performed large-scale data analyses and used them to develop a control-based migrant worker-associated susceptible-latently infected-infectious-recovered (SLTR) model. We used the SLTR model to assess potential intervention strategies such as social distancing, early screening, and directly observed treatment, short-course (DOTS) for TB transmission among migrant workers and locals in three major hotspot cities from 2018 to 2023. We showed that social distancing was the best single strategy, while the best dual measure was social distancing coupled with early screening. However, the effectiveness of the triple strategy was marginally (1-3%) better than that of the dual measure. Our study provides a mechanistic framework to facilitate understanding of TB transmission dynamics between locals and migrant workers and to recommend better prevention strategies in anticipation of achieving WHO's milestones by the next decade. Our work has implications for migrant worker-associated TB infection prevention on a global scale and provides a knowledge base for exploring how outcomes can be best implemented by alternative control measure approaches.
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Tuberculosis Latente , Migrantes , Tuberculosis , Demografía , Humanos , Taiwán/epidemiología , Tuberculosis/diagnóstico , Tuberculosis/epidemiología , Tuberculosis/prevención & controlRESUMEN
Oxytetracycline (OTC), one of the most important antibiotics in aquaculture industry, has been linked to emergence of antibiotic resistant genes in the aquatic environment. Given rapid growth of the aquaculture industry and unregulated use of antibiotics, it is necessary to implement measures to mitigate the impact of antibiotic resistance risk on environmental and human health. However, there is a lack of quantitative models to properly assess risk of antibiotic resistance associated with environmentally relevant antibiotic residues. To address this issue, here we developed a computational framework to assess antibiotic resistance risk posed by low-concentration OTC in aquaculture ponds and rivers across Taiwan regions. To this end, estimated amount of aquaculture used OTC as a crucial input parameter was incorporated into a multimedia fugacity model to predict environmental concentrations of OTC in surface water/sediment. A pharmacodynamic-based dose-response model was used to characterize the OTC concentration-antibiotic resistance relationships. The risk of antibiotic resistance selection in an aquatic environment could be assessed based on a probabilistic risk model. We also established a control measure model to manage the risks of substantial OTC-induced antibiotic resistance impacts. We found that OTC residues were likely to pose a high risk of tetracycline resistance (tetR) genes selection in aquaculture ponds among all the study basins, whereas risk of tetR genes selection in rivers experienced a variably changing fashion. We also showed that it was extremely difficult to moderate the tetR genes selection rates to less than 10% increase in aquaculture ponds situated at northeastern river basins in that the minimum reductions on OTC emission rates during spring, summer, and autumn were greater than 90%. On the other hand, water concentrations of OTC during spring and summer in southwestern rivers should be prioritized to be severely limited by reducing 67 and 25% of OTC emission rate, respectively. Overall, incorporating a computational fugacity model into a risk assessment framework can identify relative higher risk regions to provide the risk-based control strategies for public health decision-making and development of robust quantitative methods to zero-in on environment with high risk of tetR genes selection in relation to aquaculture-used pharmaceutical residues.
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Titanium dioxide (TiO2) nanoparticles (NPs) are employed as an ultraviolet filter in sunscreen products because of their high ultraviolet absorptivity. However, sunscreen sprays may pose health risks due to the toxicity of inhaled TiO2 NPs. Therefore, we estimated the potential human health risk posed by inhaled TiO2 NPs emitted from sunscreen sprays. The physiology-based lung model was employed to predict the lung TiO2 NPs burden caused by long-term exposure. A Hill-based dose-response model described the relationship between lung inflammation and TiO2 NP accumulation. The Weibull threshold model was used to estimate the threshold amount of accumulation inducing 0.5% of the maximum increase in neutrophils. The potential health risk was assessed using a hazard quotient-based probabilistic risk model. All data obtained to date indicate that application of sunscreen sprays poses no significant health risk. However, using data simulations based on the threshold criterion, we discovered that in terms of practical strategies for preventing the risks posed by inhaled TiO2 NPs emitted from spray products, the suggested daily use amount and pressing number are 40 g (95% confidence interval: 11-146 g) and 66 (18-245), respectively. In this study, we successfully translated the potential health risk of long-term exposure to NP-containing sunscreen sprays and recommendations for daily application into mechanistic insights.
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The pervasive contamination of microplastics (MPs) in freshwater ecosystems is of emerging concern. Mechanistic link between exposure and effect on assessing health risk of freshwater fish posed by environmental MPs, however, is more limited. Our study filled this gap by developing a toxicokinetic/toxicodynamic (TK/TD)-based risk assessment framework to examine health effects of zebrafish and red tilapia responses to environmental concentrations of MPs appraised with a variety of valuable published data on a global scale. We assessed organ-specific TK parameters and mean residence times for polystyrene (PS)-MPs-exposed freshwater fish in size- and concentration-dependent manners. We estimated the relatively sensitive benchmark concentrations (BMCs) of PS-MPs for oxidative stress in zebrafish and detoxification in red tilapia to be ~1.0 and ~119 µg g-1, respectively. Based on continental scale MPs trends, the high MPs concentrations were over Asia, with a mean value of 36 mg L-1. Given metabolic disturbances in zebrafish and red tilapia as bioindicators, we found that MPs pollution was highly likely to enhance fish health risks and that this factor must therefore be considered in evaluations of MPs susceptibility of freshwater fish. Our TK/TD-based risk scheme could help inform intensified efforts to mitigate environmental MPs pollution in order to benefit freshwater fish species and people who depend on healthy stocks of different fish.
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Microplásticos , Contaminantes Químicos del Agua , Animales , Asia , Ecosistema , Agua Dulce , Humanos , Plásticos/toxicidad , Medición de Riesgo , Toxicocinética , Contaminantes Químicos del Agua/toxicidadRESUMEN
Nano zero-valent iron (nZVI) is one of the most paramount nanoparticles (NPs) applied in environmental remediation, leading to great concerns for the potential impacts on soil ecosystem health. The objective of this study was to link toxicokinetics and consumer-resource dynamics in the Caenorhabditis elegans-Escherichia coli (worm-bacteria) ecosystem. The biokinetic parameters of bacteria and worms were obtained from toxicokinetic experiments and related published literature. Biomass dynamics of bacteria and worms were estimated by employing the modified Lotka-Volterra model. Dynamics of bacteria and worm biomass, internal concentrations of nZVI, bioconcentration factors (BCFs), and biomagnification factors (BMFs) were simulated based on the consumer-resource dynamics. Results showed that the biomass of worms steadily increased from 22.25 to 291.49 g L-1, whereas the biomass of bacteria decreased from 17.17 to 4.70 × 10-8 g L-1 after 96-h exposures of nZVI. We also observed ratios of nZVI concentrations in worms and bacteria increased from 0.06 to 26.60 after 96 h. Moreover, decrements of the bioconcentration factor of E. coli (BCFE) values from 0.82 to 0.03 after 96 h were observed, whereas values of BMFs increased from 0.06 to 57.62 after 96 h. Internal concentrations of nZVI in worms were found to be mainly influenced by the ingestion rate of bacteria by worms, and the biomass conversion of bacteria had the lowest effect. Implementation of the integrated bioaccumulation-consumer-resource model supports the hypothesis that the C. elegans-E. coli dynamics of internal nZVI concentrations could be effectively associated with the predator-prey behavior and was dominated by the same physiological parameter in the two biological systems.