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1.
BMC Musculoskelet Disord ; 25(1): 505, 2024 Jun 28.
Artículo en Inglés | MEDLINE | ID: mdl-38943147

RESUMEN

Crohn's disease (CD) is an inflammatory bowel disease affecting the digestive tract, the incidence of which is on the rise worldwide. The most common clinical manifestation of hemophilia is arthropathy secondary to recurrent joint effusions and chronic synovitis. This article reports on a rare 25-year-old male patient with both hemophilic arthropathy and Crohn's disease who was at risk for pathogenic gastrointestinal bleeding. After undergoing endoscopic pathologic testing and genetic testing, a multidisciplinary expert work-up of a treatment and nutritional plan was performed. The patient improved clinically and adhered to conservative treatment. This case report is the first report of this rare co-morbidity, demonstrating the highly pathogenic mutation locus and summarizing the clinical experience of early diagnosis and treatment.


Asunto(s)
Enfermedad de Crohn , Hemofilia A , Humanos , Masculino , Enfermedad de Crohn/complicaciones , Enfermedad de Crohn/diagnóstico , Adulto , Hemofilia A/complicaciones , Hemofilia A/diagnóstico , Artropatías/etiología , Artropatías/diagnóstico , Hemartrosis/etiología , Hemartrosis/diagnóstico
2.
BMC Musculoskelet Disord ; 25(1): 19, 2024 Jan 02.
Artículo en Inglés | MEDLINE | ID: mdl-38167054

RESUMEN

Osteochondral autograft transplantation (OAT) has been commonly applied in the knee and ankle while the technique has not yet been a popularity in the femoral head. In this article, we present a 28-year-old female patient, who has a history of 1-year-use of glucocorticoid in the treatment of idiopathic thrombocytopenic purpura, with steroid-induced osteonecrosis of the femoral head (SONFH). She underwent surgical hip dislocation, osteochondroplasty, OAT, and internal fixation. Her Harris Hip Score improved from 64 to 82 in 36 months to follow-up. The case is valuable considering that a single, instead of several, 1.5 cm autograft was harvested from the non-bearing part of the same femoral head. This modification dispensed with the need of surgery for harvesting autograft from knee or ankle and reduced the structural vulnerability brought by the multihole donor part of the femoral head.


Asunto(s)
Osteonecrosis , Púrpura Trombocitopénica Idiopática , Humanos , Femenino , Adulto , Cabeza Femoral/diagnóstico por imagen , Cabeza Femoral/cirugía , Autoinjertos , Trasplante Óseo/métodos , Osteonecrosis/cirugía , Resultado del Tratamiento
3.
Arch Orthop Trauma Surg ; 144(5): 2273-2281, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38615291

RESUMEN

INTRODUCTION: Following total knee arthroplasty (TKA), there is a significant decline in periprosthetic bone mineral density (BMD), potentially resulting in complications such as prosthetic loosening, periprosthetic fracture, and influencing the postoperative recovery. The objective of this study was to summarize the factors influencing periprosthetic BMD in TKA from existing studies. METHODS: A comprehensive systematic search was performed in 4 databases: Pubmed, Embase, Web of Science, and Cochrane Library. The last search was carried out on October 12, 2023. We used the keywords ''total knee arthroplasty'', ''bone mineral density'' and each of them combined with ''tibia'' and ''femur'' to identify all relevant articles reporting about potential impact factors influencing the periprosthetic BMD in patients after TKA. RESULTS: Out of 1391 articles, 22 published from 2001 to 2023 were included in this systematic review. Following eligibility screening, six significant categories affecting periprosthetic BMD were recognized: prosthesis type, design of stem, coating, body weight, cement, and peg distance. CONCLUSION: Mobile-bearing prostheses, modular polyethylene design, short stems, cruciform stems, avoidance of bone cement, higher body mass index, titanium nitride coating, and a smaller medial peg distance could potentially benefit periprosthetic BMD. Comprehensive consideration of diverse factors influencing periprosthetic BMD before surgery and collaboration with post-operative drug therapy are essential. TRIAL REGISTRY: The PROSPERO registration number is CRD42023472030.


Asunto(s)
Artroplastia de Reemplazo de Rodilla , Densidad Ósea , Prótesis de la Rodilla , Humanos , Artroplastia de Reemplazo de Rodilla/métodos , Diseño de Prótesis , Fracturas Periprotésicas/etiología , Falla de Prótesis
4.
J Environ Manage ; 328: 117003, 2023 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-36508975

RESUMEN

Concentrating solar power (CSP) is considered as a promising renewable electricity source due to its superiority in providing dispatchable and base-load electricity. This study performs a systems process analysis to quantify the carbon emissions and nonrenewable energy costs induced by a state-of-art demonstration CSP plant located in the Tibetan plateau. Estimated to induce 111.2 g CO2 eq/kWh carbon emissions and 1.42 MJ/kWh non-renewable energy consumption, the CSP plant is considered to have extremely high carbon neutrality (88.8%) and energy renewability (86.4%). The prominent performance of carbon emissions reduction and energy conservation induced by the CSP plant shed light on its superiority of reliable power supply and environmental benefits. The plant is expected to cumulatively fulfill 3.4 million tons of carbon emissions reduction over its life cycle. In contrast to coal-based power and other renewable energy technologies, CSP technology is shown to be a promising solution to the low-carbon energy transition. Besides, a scenario analysis indicates that the incremental employment of CSP technologies will play a critical role in coping with climate change and energy security in China. Moreover, multiple policies to facilitate the development of the CSP system in China are elaborated, such as the promotion of integrated solar combined-cycle systems. The empirical finding draws a holistic picture of the carbon neutrality and energy sustainability performance of CSP technologies, and the systematic analysis in this study provides comprehensive policy perspectives for energy policy in the Tibetan region as well as in China in the context of global climate change.


Asunto(s)
Energía Solar , Carbono , China , Políticas , Centrales Eléctricas , Dióxido de Carbono/análisis
5.
J Environ Manage ; 322: 116016, 2022 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-36055091

RESUMEN

Pastureland used for livestock grazing is globally much bigger than arable land. This study investigates the pastureland use embedded in global supply chains by using multi-regional systems input-output anlysis, tracing embodied pastureland use from source of exploitation to sink of final consumption in the global economy. The world's pastureland resources is shown reallocated through the supply chain mainly to the four major economies: EU, the United States, China, and Japan. These four economies are responsible for driving more than half of the global pastureland exploitation. Major supply chains responsible for the global reallocation of pastureland use include the cattle supply chain from Other Asia & Pacific to the United States, China, and Japan, and the cattle supply chain from Africa to EU and the Middle East. This paper demonstrates the nature and scale of the global reallocation of pastureland resources through the supply chain, highlighting the fact that the global shift of pastureland use from nature-based to economic-based may exacerbate ecological inequity between world regions. It is proposed that future policies and regulations should encourage sustainability goals not only on a regional level but on a global scale, finding pathways to sustainable and equitable livestock production by inter-regional collaboration.


Asunto(s)
Ganado , África , Animales , Asia , Bovinos , China , Japón
6.
J Environ Manage ; 280: 111741, 2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-33352380

RESUMEN

Land occupation by solar power installations has become a rising concern that may cause adverse impacts on natural ecosystems and biodiversity. Existing studies mainly adopt a local perspective to view land use requirements of solar power and forget that the solar-based electricity system is subordinate to the macro economy and nourished by the material, machinery and service support by various economic sectors. To manifest a key aspect of the footprint of solar power on land resources, this study uncovered the extensive industrial land use initiated by the infrastructure of a representative pilot solar-based electricity plant using a systems perspective. The results in this study show that in magnitude, land footprint by the infrastructure of the pilot solar plant amounts to three times as much as the onsite land area. Also, the land footprint calculated is revealed as one order of magnitude larger than a previous finding that includes primary materials only, and four to seven times higher than the onsite land use by coal-based electricity plants. The outcome implies that existing environmental management policies need to be re-evaluated by putting enough emphasis on the land displacement by solar power systems along the production chain.


Asunto(s)
Energía Solar , China , Ecosistema , Política Ambiental , Luz Solar
7.
J Stroke Cerebrovasc Dis ; 29(5): 104649, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-32115341

RESUMEN

OBJECTIVE: We aimed to develop an early and intense lower extremity training technique using a recumbent cycle ergometer system in patients with acute ischemic stroke. METHODS: This was a pilot, prospective, randomized, controlled study with 2 parallel groups followed for 3 months with blinded assessment of outcomes. Thirty-one eligible patients were randomized to experimental and control groups. To strengthen the motion of the lower extremities within 48 hours after stroke, the control and experimental groups received conventional treatment and additional interventions under a therapist's guidance combined with conventional treatment, respectively. The primary outcome measure was the change in lower extremity motor control from admission to 4 weeks, assessed by the Fugl-Meyer Assessment. Secondary outcomes were the number of days to walking 50 m and the change in the Berg Balance Scale score and Barthel index. The modified Rankin Score was used to assess the overall function and prognosis at 3 months. RESULTS: Fugl-Meyer Assessment and Berg Balance Scale scores and Barthel index increased over time in the experimental group, as did the Berg Balance Scale score and Barthel index in the control group (P < .001). However, Fugl-Meyer Assessment scores in the control group were similar over time (F = 2.303, P = 1.119). Fugl-Meyer Assessment scores in the experimental group were higher than those in the control group after 2 and 4 weeks (P = .084 and .037, respectively). Compared with the control group at 2 weeks or at discharge, the percentage of patients who returned to unassisted walking in the experimental group showed an increasing trend (56.3% versus 26.67%, P = .095), but there was no significant difference between the 2 groups after 3 months (P = .598). The modified Rankin Score at 3 months showed no significant difference between the 2 groups (P > .05). CONCLUSIONS: Our early and intense lower extremity training technique involving a leg cycle ergometer system contributes to the recovery of lower extremity function in patients with acute ischemic stroke. This finding will provide a basis for future investigations on the applicability of the intervention in early lower extremity and walking rehabilitation among individuals with neurological disorder.


Asunto(s)
Isquemia Encefálica/rehabilitación , Terapia por Ejercicio , Extremidad Inferior/inervación , Actividad Motora , Rehabilitación de Accidente Cerebrovascular , Accidente Cerebrovascular/terapia , Tiempo de Tratamiento , Caminata , Anciano , Ciclismo , Isquemia Encefálica/diagnóstico , Isquemia Encefálica/fisiopatología , China , Evaluación de la Discapacidad , Femenino , Humanos , Masculino , Persona de Mediana Edad , Proyectos Piloto , Equilibrio Postural , Estudios Prospectivos , Recuperación de la Función , Accidente Cerebrovascular/diagnóstico , Accidente Cerebrovascular/fisiopatología , Factores de Tiempo , Resultado del Tratamiento
8.
Zhongguo Dang Dai Er Ke Za Zhi ; 22(12): 1344-1348, 2020 Dec.
Artículo en Zh | MEDLINE | ID: mdl-33328008

RESUMEN

With the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) all over the world, there is an increasing number of children with such infection. Angiotensin-converting enzyme 2 (ACE2), one of the binding sites for SARS-CoV-2 infection in humans, can bind to viral spike proteins, allowing transmembrane serine protease (TMPRSS2) to activate S-protein to trigger infection and induce the production of various inflammatory factors such as interleukin-1, interferon-l, and tumor necrosis factor. Compared with adults, children tend to have lower expression levels of ACE2 and TMPRSS2, which are presumed to be associated with milder symptoms and fewer cases in children. The article summarizes the research advances in the role of ACE2 during SARS-CoV-2 infection, in order to help understand the pathogenic mechanism of SARS-CoV-2 and provide a reference for better development of drugs and vaccines to prevent and treat coronavirus disease 2019 in children.


Asunto(s)
Enzima Convertidora de Angiotensina 2/metabolismo , COVID-19 , Receptores Virales/metabolismo , SARS-CoV-2 , Niño , Humanos , Serina Endopeptidasas/metabolismo
9.
Biochem Biophys Res Commun ; 457(3): 391-7, 2015 Feb 13.
Artículo en Inglés | MEDLINE | ID: mdl-25582778

RESUMEN

In this study, we tested the potential role of astragaloside IV (AS-IV) against oxygen and glucose deprivation/re-oxygenation (OGD/R)-induced damages in murine cortical neurons, and studied the associated signaling mechanisms. AS-IV exerted significant neuroprotective effects against OGD/R by reducing reactive oxygen species (ROS) accumulation, thereby attenuating oxidative stress and neuronal cell death. We found that AS-IV treatment in cortical neurons resulted in NF-E2-related factor 2 (Nrf2) signaling activation, evidenced by Nrf2 Ser-40 phosphorylation, and its nuclear localization, as well as transcription of antioxidant-responsive element (ARE)-regulated genes: heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase 1 (NQO-1) and sulphiredoxin 1 (SRXN-1). Knockdown of Nrf2 through lentiviral shRNAs prevented AS-IV-induced ARE genes transcription, and abolished its anti-oxidant and neuroprotective activities. Further, we discovered that AS-IV stimulated heparin-binding-epidermal growth factor (HB-EGF) release to trans-activate epidermal growth factor receptor (EGFR) in cortical neurons. Blockage or silencing EGFR prevented Nrf2 activation by AS-IV, thus inhibiting AS-IV-mediated anti-oxidant and neuroprotective activities against OGD/R. In summary, AS-IV protects cortical neurons against OGD/R damages through activating of EGFR-Nrf2 signaling.


Asunto(s)
Corteza Cerebral/efectos de los fármacos , Corteza Cerebral/metabolismo , Receptores ErbB/metabolismo , Factor 2 Relacionado con NF-E2/metabolismo , Daño por Reperfusión/metabolismo , Saponinas/farmacología , Triterpenos/farmacología , Animales , Antioxidantes/farmacología , Corteza Cerebral/lesiones , Modelos Animales de Enfermedad , Medicamentos Herbarios Chinos/farmacología , Receptores ErbB/antagonistas & inhibidores , Receptores ErbB/genética , Técnicas de Silenciamiento del Gen , Ratones , Factor 2 Relacionado con NF-E2/antagonistas & inhibidores , Factor 2 Relacionado con NF-E2/genética , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Fármacos Neuroprotectores/farmacología , Cultivo Primario de Células , ARN Mensajero/genética , ARN Mensajero/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Daño por Reperfusión/genética , Daño por Reperfusión/prevención & control , Activación Transcripcional/efectos de los fármacos
10.
J Clin Lab Anal ; 28(4): 335-40, 2014 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-24659029

RESUMEN

BACKGROUND: The anticardiolipin antibodies (aCL) test has become a laboratory standard for the clinical diagnosis of antiphospholipid syndrome (APS). To better the quantitative detection of aCL-IgM so as to classify patients correctly and timely as APS positive, we established herein a new immunoassay based on a time-resolved fluoroimmunoassay (TRFIA). METHODS: The complex of cardiolipin plus bovine anti-ß2 glycoprotein-I was used as antigen fixed on microtiter plates to detect serum aCL-IgM, and Eu(3+) -labeled rabbit antihuman IgM was used as conjugate. The precision, sensitivity, specificity, coefficient of recovery, and stability of the assay were evaluated, and comparison with the traditional, classical enzyme-linked immunosorbent assay (ELISA) was also made. RESULTS: The detection limit of the aCL-IgM TRFIA kit we established was 0.1 MPL U/ml, with a wider detectable range than commercial ELISA ones when a strong-positive specimen was diluted from 2,630.9 to 0.08 MPL U/ml. There was a good liner range within 0.16 to 2,630.9 MPL U/ml, whereas it was within 5.14 to 328.86 MPL U/ml when using three commercial ELISA ones. The average intra- and interassay variability was 3.19 and 3.70%, respectively. The mean recovery rate was 101.95%. The clinical diagnostic specificity was 98%. Additionally, the established assay kit presented good characteristics of stability and correlated well with the ELISA, and the correlation coefficient was 0.955. CONCLUSION: The aCL-IgM TRFIA provides an approach to a more sensitive and reliable diagnosis of APS. Further validation of its use is required.


Asunto(s)
Anticuerpos Anticardiolipina/análisis , Europio , Inmunoglobulina M/análisis , Animales , Calibración , Bovinos , Ensayo de Inmunoadsorción Enzimática , Fluoroinmunoensayo , Humanos , Juego de Reactivos para Diagnóstico , Estándares de Referencia , Coloración y Etiquetado
11.
Clin Nucl Med ; 49(7): 680-682, 2024 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-38598735

RESUMEN

ABSTRACT: A 72-year-old man presented with a painless penile mass for 3 months. Contrast-enhanced CT revealed heterogeneous enhancement, whereas 18 F-FDG PET/CT displayed inhomogeneous 18 F-FDG accumulation in the lesion without other abnormal activity. The histopathological examination from biopsied specimen confirmed the diagnosis of a plasmacytoma. However, the subsequent tests, including serum/urine immunofixation electrophoresis, serum/urine free light chain assay, and bone marrow smear/biopsy, did not show any abnormalities. The conclusive diagnosis was a solitary extramedullary plasmacytoma of the penis.


Asunto(s)
Fluorodesoxiglucosa F18 , Neoplasias del Pene , Plasmacitoma , Tomografía Computarizada por Tomografía de Emisión de Positrones , Humanos , Masculino , Plasmacitoma/diagnóstico por imagen , Plasmacitoma/patología , Anciano , Neoplasias del Pene/diagnóstico por imagen , Neoplasias del Pene/patología
12.
Clin Nucl Med ; 2024 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-38768095

RESUMEN

ABSTRACT: A 72-year-old woman, who was a nonsmoker, presented with chest distress persisting for over 10 days. Plain chest CT revealed thickening of the left pleura accompanied by hydrothorax. Subsequent 18F-FDG PET/CT showed irregular thickening involving the visceral, parietal, and interlobular pleura on the left side, with diffuse high avidity of 18F-FDG. The left pleural mesothelioma was suspected initially, but pathological examination from biopsied specimen later confirmed a thoracic SMARCA4-deficient undifferentiated tumor.

13.
J Orthop Surg Res ; 19(1): 52, 2024 Jan 11.
Artículo en Inglés | MEDLINE | ID: mdl-38212803

RESUMEN

INTRODUCTION: Patients with haemophilia (PWH) may have lower bone mineral density (BMD). The risk of low BMD in PWH has not been comprehensively analysed. This study aimed to examine the risk of low BMD and changes in BMD in PWH. METHODS: A comprehensive systematic search was performed in 4 databases: PubMed, Embase, Web of Science, and Cochrane Library. The last search was carried out on 11 December 2022. Review Manager 5.4 and Stata 16 were used for meta-analysis. Odds ratios were calculated by the incidence of low BMD between the haemophilia and control groups in each study. A meta-analysis of the odds ratios for each study was performed to estimate pooled odds ratios. Fixed effects models or random effects models were used to assess outcomes. Heterogeneity was evaluated using Higgins' I2. Subgroup analysis and sensitivity analysis were performed to interpret the potential source of heterogeneity. A funnel plot, Egger's regression test, and the trim-and-fill method were used to assess publication bias. RESULTS: 19 of 793 studies, published between 2004 and 2022, that were identified by search strategy were included in this meta-analysis. The risk for low BMD was approximately four times higher compared to controls. PWH have significantly lower lumbar spine, femoral neck, and total hip BMD. Subgroup analysis showed that the risk of low BMD did not increase significantly in developed countries. Very low heterogeneity was observed in the meta-analysis of the risk of low BMD. The result from Egger's regression test suggested that there may be publication bias. However, the meta-analysis results did not alter after the trim-and-fill correction and the findings were robust. CONCLUSION: Haemophilia was associated with an increased risk of low BMD. However, the risk of low BMD did not increase significantly in developed countries. And BMD was reduced in PWH, regardless of age, region, or economic ability. For PWH, our concerns should extend beyond bleeding and osteoarthritis to encompass BMD starting at a young age.


Asunto(s)
Enfermedades Óseas Metabólicas , Hemofilia A , Osteoporosis , Humanos , Densidad Ósea , Cuello Femoral , Hemofilia A/complicaciones , Osteoporosis/complicaciones
14.
Orthop Surg ; 16(4): 802-810, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38438160

RESUMEN

Knee osteoarthritis (KOA) is widely recognized as a chronic joint disease characterized by degeneration of knee cartilage and subsequent bone hyperplasia. However, it is important to acknowledge the significant role of muscles in the development and progression of KOA. Muscle function (MF) and muscle quality (MQ) are key factors in understanding the involvement of muscles in KOA. Quantitative indices such as muscle mass, muscle strength, muscle cross-sectional area, muscle thickness, and muscle fatigue are crucial in assessing MF and MQ. Despite the growing interest in KOA, there is a scarcity of studies investigating the relationship between muscles and this condition. This review aims to examine the commonly used indices and measurement methods for assessing MF and MQ in clinical settings, while also exploring the association between muscles and KOA. Furthermore, this article highlights the importance of restoring MF and MQ to enhance symptom management and improve the quality of life for patients with KOA.


Asunto(s)
Osteoartritis de la Rodilla , Humanos , Calidad de Vida , Articulación de la Rodilla , Músculo Esquelético , Extremidad Inferior
15.
Sci Rep ; 14(1): 10943, 2024 05 13.
Artículo en Inglés | MEDLINE | ID: mdl-38740910

RESUMEN

This study aims to investigate the relationship between weight-adjusted-waist index (WWI), a new body index, and sarcopenia, while also assessing the potential of WWI as a tool for screening sarcopenic patients. The cross-sectional study involved adults who possessed complete data on WWI and appendicular skeletal muscle mass from the 1999-2006 and 2011-2018 National Health and Nutrition Examination Surveys. Weighted multivariate regression and logistic regression analyses were employed to explore the independent relationship between WWI and sarcopenia. The study included 26,782 participants. The results showed that WWI demonstrated a positive correlation with sarcopenia risk. In the fully adjusted model, with each 1 unit increase in WWI, the risk of developing sarcopenia rose 14.55 times higher among males (OR: 14.55, 95% CI 12.33, 17.15) and 2.86 times higher among females (OR: 2.86, 95% CI 2.59, 3.15). The optimal cutoff values of WWI for sarcopenia were 11.26 cm/√kg for males and 11.39 cm/√kg for females. Individuals with a higher WWI have an increased risk of developing sarcopenia, and a high WWI functions as a risk factor for sarcopenia. Assessing WWI could assist in identifying individuals at risk of sarcopenia.


Asunto(s)
Sarcopenia , Humanos , Sarcopenia/epidemiología , Sarcopenia/diagnóstico , Masculino , Femenino , Persona de Mediana Edad , Estudios Transversales , Anciano , Adulto , Factores de Riesgo , Encuestas Nutricionales , Peso Corporal , Índice de Masa Corporal
16.
Orthop Surg ; 16(3): 718-723, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38180272

RESUMEN

INTRODUCTION: Patients with hemophilia (PWH) constantly suffer hemarthrosis, which leads to deformity of the hip joint. Therefore, PWH who are going to receive total hip arthroplasty (THA) should be exclusively treated before the surgery with careful measurement of their proximal femur. Hence, we conducted a retrospective study to explore the anatomical parameters of and differences in the proximal femur in hemophilic patients who underwent THA. METHODS: The retrospective study comprised data of adult patients who received total hip arthroplasty from 2020 to 2022 in the research center. Patients having a diagnosis of hemophilic arthritis and received THA were included in experimental group, and patients with hip arthritis or femoral head necrosis were taken as control group. Parameters including femoral offset, neck-shaft angle (NSA), medullary cavity of 20 mm above mid-lesser trochanter level (T+20), mid-lesser trochanter level (T), and 20 mm blow it (T-20), and canal flare index (CFI), femoral cortical index (FCI) were measured on X-ray and CT images with PACS by two independent doctors. Data was analyzed by SPSS 20. Kolmogorov-Smirnov test was used to test data normality. Student's t-test was performed between PWH and control group. p < 0.05 was considered statistically significant. RESULTS: Among the 94 hips, 39 (41.5%) were included in group hemophilia and 55(58.5%) in control group. The mean age of the patients was 49.36 ± 12.92 years. All cases were male patients. Data demonstrated significantly smaller femoral cortical index (FCI), femoral offset, medullary cavity of 20 mm above mid-lesser trochanter level, mid-lesser trochanter level, and 20 mm below it, and neck-shaft angle (NSA) was obviously larger in PWH than control group (p < 0.05). No significant difference was found in canal flare index (CFI). CONCLUSION: Hemophilic patients undergoing THA were prone to longer and thinner proximal femur. Preoperative morphological analysis of femur is recommended.


Asunto(s)
Artritis , Artroplastia de Reemplazo de Cadera , Hemofilia A , Prótesis de Cadera , Adulto , Humanos , Masculino , Persona de Mediana Edad , Femenino , Artroplastia de Reemplazo de Cadera/métodos , Estudios Retrospectivos , Hemofilia A/complicaciones , Fémur/diagnóstico por imagen , Fémur/cirugía , Fémur/anatomía & histología , Artritis/cirugía
17.
J Immunoassay Immunochem ; 34(2): 197-207, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23537303

RESUMEN

We demonstrate herein a novel time-resolved fluoroimmunoassay (TRFIA) with high sensitivity and wide range for quantitative detection of ANA-Ig(GAM) antibodies using a biotin-avidin amplification system. The immunoassay was conducted by following procedures for a typical sandwich immunoreactions with cell nucleus form Hela and the Eu(3+)-labeled biotin combined with biotinylated mouse anti-human Ig(GAM) served as the solid nuclear antigen for ANA and the tracer, respectively. The sensitivity, specificity, and stability of the kit were evaluated and comparison with the classical enzyme-linked immunosorbent assay (ELISA) kit was also made. The average intra-assay and interassay CVs detected by the established ANA-Ig(GAM) biotin-avidin-TRFIA were 4.21% and 6.34%, respectively. The lower detection limit was 2.24 U/mL, and the mean recovery rate was 100.74%. The good measurable range of the established biotin-avidin-TRFIA was within 1.95-64,000 U/mL, while it was only within 32.5-4000 U/mL using an ELISA kit. The values determined by the biotin-avidin-TRFIA and ELISA correlated well (R2 = 0.989). The positive rate of healthy volunteers and patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), primary biliary cirrhosis (PBC), Sjögren's syndrome (SS), scleroderma, and mixed connective tissue disease (MCTD) was 0, 100%, 18.5%, 100%, 37.9%, 90.9%, and 92%, respectively. We conclude that the biotin-avidin-TRFIA we developed gives promise for greater sensitivity and accurate detection for ANA-Ig(GAM) in diagnosing and monitoring autoimmune disorders.


Asunto(s)
Anticuerpos Antinucleares/sangre , Fluoroinmunoensayo , Animales , Artritis Reumatoide/sangre , Artritis Reumatoide/diagnóstico , Avidina/química , Biotina/química , Ensayo de Inmunoadsorción Enzimática , Humanos , Cirrosis Hepática Biliar/sangre , Cirrosis Hepática Biliar/diagnóstico , Lupus Eritematoso Sistémico/sangre , Lupus Eritematoso Sistémico/diagnóstico , Ratones , Enfermedad Mixta del Tejido Conjuntivo/sangre , Enfermedad Mixta del Tejido Conjuntivo/diagnóstico , Variaciones Dependientes del Observador , Esclerodermia Sistémica/sangre , Esclerodermia Sistémica/diagnóstico , Sensibilidad y Especificidad , Síndrome de Sjögren/sangre , Síndrome de Sjögren/diagnóstico
18.
Clin Nucl Med ; 48(11): 1000-1002, 2023 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-37793165

RESUMEN

ABSTRACT: A 74-year-old man presented to the hospital complaining of weight loss, increasing fatigue, and blurred vision. The abdominal ultrasonography initially revealed a massive lesion in the mesentery, which was later confirmed by a contrast-enhanced CT scan. The 18 F-FDG PET/CT scan showed a single, solitary hypermetabolic mass. The patient was finally diagnosed with Waldenström macroglobulinemia with mesentery involvement by the histopathological examination.


Asunto(s)
Tomografía Computarizada por Tomografía de Emisión de Positrones , Macroglobulinemia de Waldenström , Masculino , Humanos , Anciano , Fluorodesoxiglucosa F18 , Macroglobulinemia de Waldenström/diagnóstico por imagen , Macroglobulinemia de Waldenström/patología , Tomografía de Emisión de Positrones , Mesenterio/diagnóstico por imagen , Mesenterio/patología
19.
J Immunoassay Immunochem ; 33(2): 156-65, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22471606

RESUMEN

The hepatitis B virus (HBV) PreS1 antigen is expressed at the distal most region of the envelope protein and contains the hepatocyte receptor-binding site. The presence of the HBV PreS1 antigen in serum and liver of HBsAg-positive patients is a new marker used for diagnosing HBV infection, and is indicative of viral replication. Our objective is to establish a method of time-resolved fluoroimmunoassay (TRFIA) with higher sensitivity and broader detection range for detecting serum HBV PreS1 antigen. Eu(3+) labeling of antibodies was performed with respective labeling kits, and Eu(3+) fluorescence intensity was measured with an auto DELFIA1235 TRFIA analyzer. The established method was evaluated for its performance. Serum specimens (574 in total) from Wuxi People's Hospital were analyzed for PreS1 antigen using the TRFIA and ELISA. The precision, specificity, and sensitivity of the TRFIA were clearly better than ELISA. The detection limit was 0.01 ng/mL. The average recovery rate for PreS1 antigens was 103.3%. There was significant correlation between the PreS1 antigen results obtained by TRFIA and ELISA in 374 serum samples with HBV >10(3) IU/mL (χ(2) = 25.04, p < 0.01) and 183 HbeAg-positive serum samples (χ(2) = 12.07, p < 0.01). Normal reference ranges were established at 0-0.32 ng/mL based on the values obtained from 100 healthy controls. TRFIA is a significantly effective method for clinical detection of serum HBV PreS1 antigens.


Asunto(s)
Antígenos de Superficie de la Hepatitis B/análisis , Antígenos de Superficie de la Hepatitis B/inmunología , Virus de la Hepatitis B/inmunología , Hepatitis B/diagnóstico , Precursores de Proteínas/análisis , Precursores de Proteínas/inmunología , ADN Viral/genética , Ensayo de Inmunoadsorción Enzimática , Fluoroinmunoensayo , Hepatitis B/inmunología , Antígenos de Superficie de la Hepatitis B/sangre , Virus de la Hepatitis B/aislamiento & purificación , Humanos , Hígado/inmunología , Hígado/virología , Precursores de Proteínas/sangre , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Sensibilidad y Especificidad , Factores de Tiempo , Replicación Viral/inmunología
20.
Clin Nucl Med ; 47(4): e340-e343, 2022 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-34619697

RESUMEN

ABSTRACT: A 47-year-old woman presented with a large mass in the upper inner quadrant of the left breast. 18F-FDG PET/CT revealed a low-density left breast mass with heterogeneous ring-like 18F-FDG uptake. Core needle biopsy of the tumor revealed a rare subtype of metaplastic breast carcinoma, matrix-producing carcinoma. The tumor size slightly decreased after 6 cycles of neoadjuvant chemotherapy with docetaxel and epirubicin. The left breast was entirely resected subsequently, and the matrix-producing carcinoma of the breast was ultimately diagnosed.


Asunto(s)
Neoplasias de la Mama , Carcinoma , Neoplasias de la Mama/patología , Femenino , Fluorodesoxiglucosa F18 , Humanos , Persona de Mediana Edad , Tomografía Computarizada por Tomografía de Emisión de Positrones , Radiofármacos/uso terapéutico
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