RESUMEN
OBJECTIVE: To establish HPLC fingerprint of Hedyotis chrysotricha. METHODS: Hypersil BDS C18 (250 mm x 4.6 mm, 5 microm) column was used with methanol and 0.1% acetic acid solution as mobile phase in gradient mode, the flow rate was 1.0 mL/ min. The detection wavelength was 345 nm and the temperature of the column was set at 30 degrees C. Using ursolic acid as the reference standard, the mutual mode of Hedyotis chrysotricha containing nine common peaks was set up. The similarity of Hedyotis chrysotricha from different habitats was analyzed. RESULTS: The fingerprints of Hedyotis chrysotricha from different habitats were similar, but also had habitat characteristics. CONCLUSION: The HPLC fingerprint can be used for identification and quality control of Hedyotis chrysotricha.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Medicamentos Herbarios Chinos/química , Hedyotis/química , Plantas Medicinales/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Hedyotis/crecimiento & desarrollo , Plantas Medicinales/crecimiento & desarrollo , Control de Calidad , Reproducibilidad de los ResultadosRESUMEN
OBJECTIVE: To establish an HPLC fingerprint of Calendula officinalis flower for its quality control. METHODS: Hypersil ODS C18 column (250 mm x 4.6 mm, 5 µm) was used with acetonitrile and water as mobile phase in a gradient mode at the flow rate of 1.0 mL/min. The detection wavelength was 220 nm and the temperature of column was set at 35 degrees C. The similarity was analyzed with the Estimating System of Similarity on the Chinese Medicine Fingerprint Chromatogram. RESULTS: The HPLC fingerprint of Calendula officinalis flower containing eleven peaks was set up. The similarity of Calendula officinalis flower from different habitats was greater than 0.90. CONCLUSION: This method is easy and reliable, which can be used to judge the habitat and control the quality of Calendula officinalis flower.