[Urgency and improvement of sanitary and helminthological techniques of soil investigation].

From its inception, sanitary parasitology aimed at protecting the health of the population. But the procedures for sanitary and parasitological studies were not always so simple and effective as it was necessary. This article presents an analysis of the evolution of helminthological knowledge and the methods of soil investigation.

[Parasitological monitoring as part of epidemiological surveillance of helminthiasis in the Russian Federation].

The paper presents data on the prevalence of helminthiasis in Russia on the basis of the State Reports on the sanitary and epidemiological situation in the Russian Federation. It comparatively characterizes morbidity rates in 2010-2012. Morbidity analysis has revealed the main reasons for the prevalence of parasitic diseases and the possible ways of their elimination.

[Global eradication of dracunculiasis is a reality nowadays].

Global efforts to eradicate dracunculiasis have continued to progress with only 542 cases reported in 2012 versus almost 1 million cases in the 1980s when the initiative was launched. The disease is still endemic, but in only four countries: South Sudan (541 cases), Chad (10 cases), Mali (4 cases), and Ethiopia (4 cases). The International Commission for the Certification of Dracunculiasis Eradication declared that 180 countries were free of the disease by 2013. Dracunculiasis was first eradicated in the former USSR in 1931 when its last case was registered due to the successful implementation of a package of appropriate measures (case detection and management, vector control, and health education, including water filtration from open water sources and behavioral changes). These approaches were actively taken into account when developing the global strategy for dracunculiasis. The World Health Organization has set a goal to eradicate the disease by 2015. However, despite the fact that the global situation looks very optimistic, the time for completing the program will be most likely postponed due to a few operational challenges remaining in the still endemic countries, in South Sudan in particular.

[Comparative analysis of the efficiency of methods for the diagnosis of cryptosporidiosis].

The paper comparatively analyzes the efficiency of two methods for the diagnosis ofcryptosporidiosis: microscopy of Ziehl-Neelsen- or Romanovsky-Gimse-stained smears and immunofluorescence labeling using the diagnostic reagent kit Crypt-a-Glo A400 BIOT (Cryptosporidium oocysts) (Stailab Co.) in fecal samples. The efficiency of the reagent kit Crypt-a-Glo A400 BIOT has been shown to be sufficiently high in diagnosing cryptosporidiosis. The authors propose to use the immunofluorescence labeling [a diagnostic reagent kit Crypt-a-Glo A400 BIOT (Cryptosporidium oocysts)] to increase the detection rate of persons suspected as having cryptosporidiosis who are invaded during comprehensive examination.

[Atopic dermatitis concurrent with toxoplasmosis in the Republic of Dagestan: epidemiological aspects].

[By using a group of patients with atopic dermatitis who live in Dagestan as an example, the authors show the high prevalence of toxoplasmosis among them. This requires new approaches to diagnosing and treating atopic dermatitis to be introduced in this region]

[Human dirofilariasis in the Tula region].

The paper describes cases of dirofilariasis in the residents of Tula and the Tula Region. One of the cases does not fit into the traditional concepts. On excising a tumor in an adolescent, two live actively moving nonencapsulated helminths: a male and a female Dirofilaria repens worms were removed, which were identified at the parasitology laboratory, Center of Hygiene and Epidemiology in the Tula Region.

[Biological characteristics of the causative agent of dirofilariasis in dogs in the Rostov Region].

This paper assesses the trends in the identification of different Dirofilaria species in the Rostov Region and comparatively analyzes the general clinical and biochemical blood indices in dogs with and Without helminthism. Seasonal variations in the invasion and species composition of the parasite have been established. At present, there is a gradual replacement of the species Dirofilaria repens by Dirofilaria immitis in this area. The blood general clinical and biochemical studies in ill dogs with dirofilariasis have ascertained that the pathogenic action on the animals is more pronounced with Dirofilaria immitis being parasitizing and with the mixed invasion of two helminth species.

[Evaluation of the efficiency of multiple coproprological study for the diagnosis of giardiasis].

Reformation of public health system financing in Russia requires the use and distribution of the most effective and least expensive diagnostic and medical methods. Giardiasis is the most common human anthroponosis. The diagnosis and treatment of this disease call for considerable funds of the budget, medical insurance companies, and the patient himself/herself. The paper gives the results of the clinicoeconomic analysis aimed at detecting the most rational frequency of diagnostic studies. The analysis provides evidence that triple coproprological study has the greatest advantages. The findings make it possible to recommend just this study for wide use in the diagnosis ofgiardiasis.

[Experimental grounds of a new concept of Opisthorchis felineus infection formation in a definitive host].

In the course of experiments in was found out that golden hamsters having the bilious duct operationally blocked display receptivity to the infection with the non-excysted Opisthorchis felineus metacercariae passed to the stomach. Excysted metacercariae injected to the system of the portal vein settle down in the bilious pathways of the liver and develop there up to the adult stage. In vitro, the metacercariae survive in the blood serum of the intact golden hamsters during one day. Based on the experiments, it is hypothesized that the early stage of O. felineus infection in the bilious duct of definitive hosts is performed by means of hematogenic migration of metacercariae through the portal veins system from the mucous layer of the alimentary tract of the host.

[The specific features of development of Echinococcus granulosus metacestode (exogenous blastogenesis) in man].

The paper describes a case of abnormal development of a parasitic cyst by the type characteristic of alveolar echinococcosis (exogenous blastogenesis) detected in a female patient operated on for echinococcus. The fact that the exogenous blastogenesis has been found allows the mechanism of a postoperative recurrence of human echinococcosis to be explained from basically new standpoints. A parasitological analysis of the punctates of cystic masses and cysts themselves, taken from patients operated on for cystic echinococcosis has revealed one of the signs of malignant exogenous proliferation of elements of a parasytic metacestode, namely, the development of acephalocysts from the germinative cells of the pedicle of protoscoleces. The capacity of cystic echinococcosis metacestodes for exogenous blastogenesis by the type of human alveolar echinococcosis is an early unknown species-specific feature of E. granulosus.

[Developing of new anthelmintics. Evaluation of fluzamide on the new experimental models of intestinal cestode infections]. / Razrabotka novykh otechestvennykh antigel'mintikov. Otsenka terapevtitseskoi aktivnosti fluzamida na novykh éksperimental'nykh modeliakh kishechnykh tsestodozov.

The efficacy of the original drug fluzamide (a N,S-containing heterocycle derivative) was evaluated by primary screening on a modified model of monoinvasion with the luminal form of Echinococcus multilocularis and on an original model of intestinal mixed invasion of E. multilocularis and Hymenolepsis nana at the immature and mature stages of the parasites in golden hamsters when an experimental host was immunosuppressed with hydrocortisone. The efficacy of fluzamide in an oral dose of 0.3 g/kg against young E. multilocularis and H. nana (the duration of their invasion was 7 days) was 99.8-100%; that against adult cestodes of both species (their maximum age was 28 days) was 100%. The original method of simulation of intestinal cestodiases in laboratory rodents, which is based on the artificial transplantation of cestodes at the prepatent stage of development from the intestine of infected donors to the intestine of healthy recipients through the latter' stomach during drug immunosuppression, showed its high reliability. There is evidence that transplanted immature strobilar cestodes can survive in the stomach, get acclimated, and continue to develop in the recipients' small bowel up to the stage of formation of infective eggs.