RESUMEN
Ultrastructural study of early subcultures and established lines of human malignant melanocytes derived from primary tumors showed that melanocytes passed through a phase of dedifferentiation during which they took on a fibroblast-like appearance; then they had a phase of spontaneous redifferentiation. The fibroblast-like cells were characterized by the presence of network-like organelles in their cytoplasm, and the melanocytes by melanosomes.
Asunto(s)
Melanocitos/ultraestructura , Melanoma/ultraestructura , Neoplasias Cutáneas/ultraestructura , Diferenciación Celular , Línea Celular , Citoplasma/ultraestructura , Humanos , Melaninas/metabolismo , Melanocitos/metabolismo , Melanoma/metabolismo , Neoplasias Cutáneas/metabolismoRESUMEN
Cells from three lines of cultured human malignant melanomas were heterotransplanted into nude mice and then recultered. The shape of the cells, the aspect of the melanosomes, and the content of 5-S-cyteinyldopa showed pronounced changes induced by the transplantation. Such results indicate that this experimental model should be used with great caution. A relationship was found between the shape of the melanosomes and the content of 5-S-cysteinyldopa in the cells.