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1.
Small ; 17(9): e1902138, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31441230

RESUMEN

An all-solid-state lithium battery based on a sulfide electrolyte is one of the most promising next-generation energy storage systems. However, the high interfacial impedance, particularly due to the internal pores in the electrode or electrolyte layers, is the major limiting factor to the development of sheet-type all-solid-state batteries. In this study, a low-resistance integrated all-solid composite electrode is developed using a hybrid of a pyrrolidinium-based ionic liquid and a polyethylene oxide polymer with lithium salt as a multifunctional interphase material, which is engineered to be compatible with the sulfide electrolyte as well as the fabrication process of sheet-type composite electrode. The interphase material fills the pore in the composite sheet while binding the components together, which effectively increases the interfacial contact area and strengthens the physical network between the components, thereby enabling enhanced ion transport throughout the electrode. The interphase-engineered sheet-type LiNi0.8 Co0.1 Mn0.1 O2 /Li10 GeP2 S12 electrode shows a high reversible capacity of 166 mAh g-1 at 25 °C, corresponding to 92% of the observed capacity in a current liquid-based cathode system, as well as enhanced cycle and rate performances. This study proposes a novel and practical method for the development of high-performance sheet-type all-solid-state lithium batteries.

2.
Phys Chem Chem Phys ; 23(9): 5438-5446, 2021 Mar 11.
Artículo en Inglés | MEDLINE | ID: mdl-33646232

RESUMEN

In this study, we present improved power characteristics and suppressed phase transition by incorporating elemental doping into a P2-type cathode of sodium ion batteries. A Cu-doped Fe-Mn based P2-type Na0.67Cu0.125Fe0.375Mn0.5O2 cathode was designed based on the calculations of the electronic structure and then examined experimentally. Using first principles, we introduced instrinsic p-type conductivity by elemental doping with Cu. Introduction of Cu generated electron holes above the Fermi level in the electronic structure, which is typical of p-type semiconductors. Charge analyses suggested that the hole generation was driven primarily by the greater reduced characteristics of Cu as compared with those of Fe and Mn. In addition, introduction of Cu retaining high reduced property also suppressed phase transition from the P2 to Z phase by Fe migration to empty Na layers mainly. Electrochemical experiments revealed improved power characteristics upon the introduction of p-type conductivity. This could be attributed to the increase in the electronic conductivity by hole generation in the valence band. This study suggests that the introduction of p-type conductivity could be a rational tactic for the development of promising cathode materials for high performance sodium ion batteries.

3.
Int J Mol Sci ; 22(11)2021 May 25.
Artículo en Inglés | MEDLINE | ID: mdl-34070397

RESUMEN

Bacterial communities in rhizosphere and root nodules have significant contributions to the growth and productivity of the soybean (Glycine max (L.) Merr.). In this report, we analyzed the physiological properties and dynamics of bacterial community structure in rhizosphere and root nodules at different growth stages using BioLog EcoPlate and high-throughput sequencing technology, respectively. The BioLog assay found that the metabolic capability of rhizosphere is in increasing trend in the growth of soybeans as compared to the bulk soil. As a result of the Illumina sequencing analysis, the microbial community structure of rhizosphere and root nodules was found to be influenced by the variety and growth stage of the soybean. At the phylum level, Actinobacteria were the most abundant in rhizosphere at all growth stages, followed by Alphaproteobacteria and Acidobacteria, and the phylum Bacteroidetes showed the greatest change. But, in the root nodules Alphaproteobacteria were dominant. The results of the OTU analysis exhibited the dominance of Bradyrhizobium during the entire stage of growth, but the ratio of non-rhizobial bacteria showed an increasing trend as the soybean growth progressed. These findings revealed that bacterial community in the rhizosphere and root nodules changed according to both the variety and growth stages of soybean in the field.


Asunto(s)
Bacterias , Glycine max , Nodulación de la Raíz de la Planta , Raíces de Plantas , Rizosfera , Microbiología del Suelo , Bacterias/clasificación , Bacterias/crecimiento & desarrollo , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/microbiología , Glycine max/crecimiento & desarrollo , Glycine max/microbiología
4.
Int J Mol Sci ; 22(18)2021 Sep 14.
Artículo en Inglés | MEDLINE | ID: mdl-34576101

RESUMEN

Near-infrared spectroscopy (NIRS) has become a more popular approach for quantitative and qualitative analysis of feeds, foods and medicine in conjunction with an arsenal of chemometric tools. This was the foundation for the increased importance of NIRS in other fields, like genetics and transgenic monitoring. A considerable number of studies have utilized NIRS for the effective identification and discrimination of plants and foods, especially for the identification of genetically modified crops. Few previous reviews have elaborated on the applications of NIRS in agriculture and food, but there is no comprehensive review that compares the use of NIRS in the detection of genetically modified organisms (GMOs). This is particularly important because, in comparison to previous technologies such as PCR and ELISA, NIRS offers several advantages, such as speed (eliminating time-consuming procedures), non-destructive/non-invasive analysis, and is inexpensive in terms of cost and maintenance. More importantly, this technique has the potential to measure multiple quality components in GMOs with reliable accuracy. In this review, we brief about the fundamentals and versatile applications of NIRS for the effective identification of GMOs in the agricultural and food systems.


Asunto(s)
Plantas Modificadas Genéticamente/fisiología , Espectroscopía Infrarroja Corta , Productos Agrícolas/fisiología , Alimentos
5.
Int J Mol Sci ; 23(1)2021 Dec 25.
Artículo en Inglés | MEDLINE | ID: mdl-35008646

RESUMEN

In recent years, the rapid development of genetically modified (GM) technology has raised concerns about the safety of GM crops and foods for human health and the ecological environment. Gene flow from GM crops to other crops, especially in the Brassicaceae family, might pose a threat to the environment due to their weediness. Hence, finding reliable, quick, and low-cost methods to detect and monitor the presence of GM crops and crop products is important. In this study, we used visible near-infrared (Vis-NIR) spectroscopy for the effective discrimination of GM and non-GM Brassica napus, B. rapa, and F1 hybrids (B. rapa X GM B. napus). Initially, Vis-NIR spectra were collected from the plants, and the spectra were preprocessed. A combination of different preprocessing methods (four methods) and various modeling approaches (eight methods) was used for effective discrimination. Among the different combinations, the Savitzky-Golay and Support Vector Machine combination was found to be an optimal model in the discrimination of GM, non-GM, and hybrid plants with the highest accuracy rate (100%). The use of a Convolutional Neural Network with Normalization resulted in 98.9%. The same higher accuracy was found in the use of Gradient Boosted Trees and Fast Large Margin approaches. Later, phenolic acid concentration among the different plants was assessed using GC-MS analysis. Partial least squares regression analysis of Vis-NIR spectra and biochemical characteristics showed significant correlations in their respective changes. The results showed that handheld Vis-NIR spectroscopy combined with chemometric analyses could be used for the effective discrimination of GM and non-GM B. napus, B. rapa, and F1 hybrids. Biochemical composition analysis can also be combined with the Vis-NIR spectra for efficient discrimination.


Asunto(s)
Brassica napus/genética , Brassica rapa/genética , Hibridación Genética/genética , Plantas Modificadas Genéticamente/genética , Quimiometría/métodos , Productos Agrícolas/genética , Flujo Génico/genética , Aprendizaje Automático , Espectroscopía Infrarroja Corta/métodos
6.
Proc Natl Acad Sci U S A ; 111(2): 599-604, 2014 Jan 14.
Artículo en Inglés | MEDLINE | ID: mdl-24379365

RESUMEN

Sodium ion batteries offer promising opportunities in emerging utility grid applications because of the low cost of raw materials, yet low energy density and limited cycle life remain critical drawbacks in their electrochemical operations. Herein, we report a vanadium-based ortho-diphosphate, Na7V4(P2O7)4PO4, or VODP, that significantly reduces all these drawbacks. Indeed, VODP exhibits single-valued voltage plateaus at 3.88 V vs. Na/Na(+) while retaining substantial capacity (>78%) over 1,000 cycles. Electronic structure calculations reveal that the remarkable single plateau and cycle life originate from an intermediate phase (a very shallow voltage step) that is similar both in the energy level and lattice parameters to those of fully intercalated and deintercalated states. We propose a theoretical scheme in which the reaction barrier that arises from lattice mismatches can be evaluated by using a simple energetic consideration, suggesting that the presence of intermediate phases is beneficial for cell kinetics by buffering the differences in lattice parameters between initial and final phases. We expect these insights into the role of intermediate phases found for VODP hold in general and thus provide a helpful guideline in the further understanding and design of battery materials.


Asunto(s)
Difosfatos/química , Suministros de Energía Eléctrica , Compuestos de Vanadio/química , Cristalografía , Electroquímica , Cinética , Modelos Teóricos , Difracción de Rayos X
7.
Phys Chem Chem Phys ; 17(4): 2388-93, 2015 Jan 28.
Artículo en Inglés | MEDLINE | ID: mdl-25490705

RESUMEN

As a means of enhancing the electrochemical performance of silicon-graphite composites, we propose a novel binder candidate that is modified by a combination of rigid and elastic functional groups on its binder framework. To provide an efficient binder that is also capable of rapid volume changes, a co-polymer binder (PAA-PAA/PMA) is synthesized by employing poly(acrylic acid) (PAA) as the main binder framework and poly(acrylic acid)-co-poly(maleic acid) (PAA/PMA) as an additional elastic polymer auxiliary. This co-polymer binder (PAA-PAA/PMA) affords a good balance of adhesive and mechanical (rigidity and elasticity) properties, which creates an excellent cycle performance with a high specific capacity (751.1 mA h g(-1)) and considerable capacity retention (64.9%) after 300 cycles. This is attributed to the ability of the added elastic functional group to respond flexibly to volume changes, thereby enhancing the overall uniformity of the electrode and ensuring a consistent electronic network. On the basis of these findings, it is considered that embedding an elastic functional group into the binder framework is an effective approach to improve the overall performance of Si-graphite composite electrodes.

8.
Angew Chem Int Ed Engl ; 54(50): 15094-9, 2015 Dec 07.
Artículo en Inglés | MEDLINE | ID: mdl-26474337

RESUMEN

The phase transition of layered manganese oxides to spinel phases is a well-known phenomenon in rechargeable batteries and is the main origin of the capacity fading in these materials. This spontaneous phase transition is associated with the intrinsic properties of manganese, such as its size, preferred crystal positions, and reaction characteristics, and it is therefore very difficult to avoid. The introduction of crystal water by an electrochemical process enables the inverse phase transition from spinel to a layered Birnessite structure. Scanning transmission electron microscopy can be used to directly visualize the rearrangement of lattice atoms, the simultaneous insertion of crystal water, the formation of a transient structure at the phase boundary, and layer-by-layer progression of the phase transition from the edge. This research indicates that crystal water intercalation can reverse phase transformation with thermodynamically favored directionality.

9.
J Am Chem Soc ; 136(22): 7961-71, 2014 Jun 04.
Artículo en Inglés | MEDLINE | ID: mdl-24813064

RESUMEN

As a subset of the metal-organic frameworks, zeolitic imidazolate frameworks (ZIFs) have potential use in practical separations as a result of flexible yet reliable control over their pore sizes along with their chemical and thermal stabilities. Among many ZIF materials, we explored the effect of thermal treatments on the ZIF-7 structure, known for its promising characteristics toward H2 separations; the pore sizes of ZIF-7 (0.29 nm) are desirable for molecular sieving, favoring H2 (0.289 nm) over CO2 (0.33 nm). Although thermogravimetric analysis indicated that ZIF-7 is thermally stabile up to ~400 °C, the structural transition of ZIF-7 to an intermediate phase (as indicated by X-ray analysis) was observed under air as guest molecules were removed. The transition was further continued at higher temperatures, eventually leading toward the zinc oxide phase. Three types of ZIF-7 with differing shapes and sizes (~100 nm spherical, ~400 nm rhombic-dodecahedral, and ~1300 nm rod-shaped) were employed to elucidate (1) thermal structural transitions while considering kinetically relevant processes and (2) discrepancies in the N2 physisorption and CO2 adsorption isotherms. The largest rod-shaped ZIF-7 particles showed a delayed thermal structural transition toward the stable zinc oxide phase. The CO2 adsorption behaviors of the three ZIF-7s, despite their identical crystal structures, suggested minute differences in the pore structures; in particular, the smaller spherical ZIF-7 particles provided reversible CO2 adsorption isotherms at ~30-75 °C, a typical temperature range of flue gases from coal-fired power plants, in contrast to the larger rhombic-dodecahedral and rod-shaped ZIF-7 particles, which exhibited hysteretic CO2 adsorption/desorption behavior.


Asunto(s)
Dióxido de Carbono/química , Imidazoles/química , Zeolitas/química , Adsorción , Cromatografía en Gel , Hidrógeno/química , Termodinámica
10.
Nano Lett ; 12(12): 6358-65, 2012 Dec 12.
Artículo en Inglés | MEDLINE | ID: mdl-23145851

RESUMEN

Spinel-structured lithium manganese oxide (LiMn(2)O(4)) cathodes have been successfully commercialized for various lithium battery applications and are among the strongest candidates for emerging large-scale applications. Despite its various advantages including high power capability, however, LiMn(2)O(4) chronically suffers from limited cycle life, originating from well-known Mn dissolution. An ironical feature with the Mn dissolution is that the surface orientations supporting Li diffusion and thus the power performance are especially vulnerable to the Mn dissolution, making both high power and long lifetime very difficult to achieve simultaneously. In this investigation, we address this contradictory issue of LiMn(2)O(4) by developing a truncated octahedral structure in which most surfaces are aligned to the crystalline orientations with minimal Mn dissolution, while a small portion of the structure is truncated along the orientations to support Li diffusion and thus facilitate high discharge rate capabilities. When compared to control structures with much smaller dimensions, the truncated octahedral structure as large as 500 nm exhibits better performance in both discharge rate performance and cycle life, thus resolving the previously conflicting aspects of LiMn(2)O(4).

11.
Adv Sci (Weinh) ; 10(5): e2204942, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36507619

RESUMEN

Compared with conventional liquid electrolytes, solid electrolytes can better improve the safety properties and achieve high-energy-density Li-ion batteries. Sulfide-based solid electrolytes have attracted significant attention owing to their high ionic conductivities, which are comparable to those of their liquid counterparts. Among them, Li thiophosphates, including Li-argyrodites, are widely studied. In this study, Li thiophosphate solid electrolytes containing BH4 - anions are prepared via a simple and fast milling method even without heat treatment. The synthesized materials exhibit a high ionic conductivity of up to 11 mS cm-1 at 25 °C, which is much higher than reported values. To elucidate the mechanism behind, the thiophosphate local structure, whose effect on the ionic conductivity remains unclear to date, is investigated. Raman and solid-state NMR spectroscopies are performed to identify the thiophosphate local structure in the sulfide samples. Based on the analysis results, the ratios of the different thiophosphate units in the prepared electrolyte samples are determined. It is found that the thiophosphate local structure can be varied by changing the amount of LiBH4 and the milling conditions, which significantly impact the ionic conductivity. The all-solid-state cell with the prepared solid electrolyte exhibits superior cycle and rate performances.

12.
ACS Appl Mater Interfaces ; 15(8): 10744-10751, 2023 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-36787511

RESUMEN

This study validates the beneficial role of residual Li compounds on the surface of Ni-rich cathode materials (LiNixCoyMnzO2, NCM). Residual Li compounds on Ni-rich NCM are naturally formed during the synthesis procedure, which degrades the initial Coulombic efficiency and generates slurry gelation during electrode fabrication in Li-ion batteries (LIBs) using liquid electrolytes. To solve this problem, washing pretreatment is usually introduced to remove residual Li compounds on the NCM surface. In contrast to LIBs, we found that residual Li compounds can serve as a functional layer that suppresses the interfacial side reactions of the NCM in all-solid-state batteries (ASSBs). The formation of resistive phosphate-based compounds from the undesirable side reaction during the initial charging step is suppressed by the residual Li compounds on the surface of the NCM, thereby reducing polarization growth in ASSBs and enhancing rate performances. The advantageous effects of the intrinsic residual Li compounds on the NCM surface suggest that the essential washing process of the NCM for the liquid-based LIB system should be reconsidered for ASSB systems.

13.
ACS Nano ; 17(16): 15931-15941, 2023 Aug 22.
Artículo en Inglés | MEDLINE | ID: mdl-37548961

RESUMEN

For realizing all-solid-state batteries (ASSBs), it is highly desirable to develop a robust solid electrolyte (SE) that has exceptional ionic conductivity and electrochemical stability at room temperature. While argyrodite-type Li6PS5Cl (LPSCl) SE has garnered attention for its relatively high ionic conductivity (∼3.19 × 10-3 S cm-1), it tends to emit hydrogen sulfide (H2S) in the presence of moisture, which can hinder the performance of ASSBs. To address this issue, researchers are exploring approaches that promote structural stability and moisture resistance through elemental doping or substitution. Herein, we suggest using zeolite imidazolate framework-8 as a moisture absorbent in LPSCl without modifying the structure of the SE or the electrode configuration. By incorporating highly ordered porous materials, we demonstrate that ASSBs configured with LPSCl SE display stable cyclability due to effective and long-lasting moisture absorption. This approach not only improves the overall quality of ASSBs but also lays the foundation for developing a moisture-resistant sulfide electrolyte.

14.
ACS Appl Mater Interfaces ; 15(50): 58367-58376, 2023 Dec 20.
Artículo en Inglés | MEDLINE | ID: mdl-38079499

RESUMEN

Halide solid electrolytes (SEs) have been highlighted for their high-voltage stability. Among the halide SEs, the ionic conductivity has been improved by aliovalent metal substitutions or choosing a ccp-like anion-arranged monoclinic structure (C2/m) over hcp- or bcc-like anion-arranged structures. Here, we present a new approach, hard-base substitution, and its underlying mechanism to increase the ionic conductivity of halide SEs. The oxygen substitution to Li2ZrCl6 (trigonal, hcp) increased the ionic conductivity from 0.33 to 1.3 mS cm-1 at Li3.1ZrCl4.9O1.1 (monoclinic, ccp), while the sulfur and fluorine substitutions were not effective. A systematic comparison study revealed that the energetic stabilization of interstitial sites for Li migration plays a key role in improving the ionic conductivity, and the ccp-like anion sublattice is not sufficient to achieve high ionic conductivity. We further examined the feasibility of the oxyhalide SE for practical and all-solid-state battery applications.

15.
J Am Chem Soc ; 134(28): 11740-8, 2012 Jul 18.
Artículo en Inglés | MEDLINE | ID: mdl-22720717

RESUMEN

As an attempt to develop lithium ion batteries with excellent performance, which is desirable for a variety of applications including mobile electronics, electrical vehicles, and utility grids, the battery community has continuously pursued cathode materials that function at higher potentials with efficient kinetics for lithium insertion and extraction. By employing both experimental and theoretical tools, herein we report multicomponent pyrophosphate (Li(2)MP(2)O(7), M = Fe(1/3)Mn(1/3)Co(1/3)) cathode materials with novel and advantageous properties as compared to the single-component analogues and other multicomponent polyanions. Li(2)Fe(1/3)Mn(1/3)Co(1/3)P(2)O(7) is formed on the basis of a solid solution among the three individual transition-metal-based pyrophosphates. The unique crystal structure of pyrophosphate and the first principles calculations show that different transition metals have a tendency to preferentially occupy either octahedral or pyramidal sites, and this site-specific transition metal occupation leads to significant improvements in various battery properties: a single-phase mode for Li insertion/extraction, improved cell potentials for Fe(2+)/Fe(3+) (raised by 0.18 eV) and Co(2+)/Co(3+) (lowered by 0.26 eV), and increased activity for Mn(2+)/Mn(3+) with significantly reduced overpotential. We reveal that the favorable energy of transition metal mixing and the sequential redox reaction for each TM element with a sufficient redox gap is the underlying physical reason for the preferential single-phase mode of Li intercalation/deintercalation reaction in pyrophosphate, a general concept that can be applied to other multicomponent systems. Furthermore, an extremely small volume change of ~0.7% between the fully charged and discharged states and the significantly enhanced thermal stability are observed for the present material, the effects unseen in previous multicomponent battery materials.

16.
Adv Mater ; 34(16): e2200083, 2022 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-35196412

RESUMEN

The wet-chemical processability of sulfide solid electrolytes (SEs) provides intriguing opportunities for all-solid-state batteries. Thus far, sulfide SEs are wet-prepared either from solid precursors suspended in solvents (suspension synthesis) or from homogeneous solutions using SEs (solution process) with restricted composition spaces. Here, a universal solution synthesis method for preparing sulfide SEs from precursors, not only Li2 S, P2 S5 , LiCl, and Na2 S, but also metal sulfides (e.g., GeS2 and SnS2 ), fully dissolved in an alkahest: a mixture solvent of 1,2-ethylenediamine (EDA) and 1,2-ethanedithiol (EDT) (or ethanethiol). Raman spectroscopy and theoretical calculations reveal that the exceptional dissolving power of EDA-EDT toward GeS2 is due to the nucleophilicity of the thiolate anions that is strong enough to dissociate the GeS bonds. Solution-synthesized Li10 GeP2 S12 , Li6 PS5 Cl, and Na11 Sn2 PS12 exhibit high ionic conductivities (0.74, 1.3, and 0.10 mS cm-1 at 30 °C, respectively), and their application for all-solid-state batteries is successfully demonstrated.

17.
Genes (Basel) ; 12(5)2021 04 25.
Artículo en Inglés | MEDLINE | ID: mdl-33923067

RESUMEN

In plants, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a main enzyme in the glycolytic pathway. It plays an essential role in glycerolipid metabolism and response to various stresses. To examine the function of PsGAPDH (Pleurotus sajor-caju GAPDH) in response to abiotic stress, we generated transgenic rice plants with single-copy/intergenic/homozygous overexpression PsGAPDH (PsGAPDH-OX) and investigated their responses to salinity stress. Seedling growth and germination rates of PsGAPDH-OX were significantly increased under salt stress conditions compared to those of the wild type. To elucidate the role of PsGAPDH-OX in salt stress tolerance of rice, an Illumina HiSeq 2000 platform was used to analyze transcriptome profiles of leaves under salt stress. Analysis results of sequencing data showed that 1124 transcripts were differentially expressed. Using the list of differentially expressed genes (DEGs), functional enrichment analyses of DEGs such as Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were performed. KEGG pathway enrichment analysis revealed that unigenes exhibiting differential expression were involved in starch and sucrose metabolism. Interestingly, trehalose-6-phosphate synthase (TPS) genes, of which expression was enhanced by abiotic stress, showed a significant difference in PsGAPDH-OX. Findings of this study suggest that PsGAPDH plays a role in the adaptation of rice plants to salt stress.


Asunto(s)
Gliceraldehído-3-Fosfato Deshidrogenasa (Fosforilante)/genética , Oryza/genética , Proteínas de Plantas/genética , Estrés Salino , Transcriptoma , Regulación de la Expresión Génica de las Plantas , Gliceraldehído-3-Fosfato Deshidrogenasa (Fosforilante)/metabolismo , Oryza/metabolismo , Hojas de la Planta/genética , Hojas de la Planta/metabolismo , Proteínas de Plantas/metabolismo , Regulación hacia Arriba
18.
Genes (Basel) ; 12(10)2021 10 09.
Artículo en Inglés | MEDLINE | ID: mdl-34680989

RESUMEN

Low temperature is a critical environmental factor restricting the physiology of organisms across kingdoms. In prokaryotes, cold shock induces the expression of various genes and proteins involved in cellular processes. Here, a cold-shock protein (ArCspA) from the South Pole-dwelling soil bacterium Arthrobacter sp. A2-5 was introduced into rice, a monocot model plant species. Four-week-old 35S:ArCspA transgenic rice plants grown in a cold chamber at 4 °C survived for 6 days. Cold stress significantly decreased the chlorophyll content in WT plants after 4 days compared with that in 35S:ArCspA transgenic plants. RNA-seq analysis was performed on WT and 35S:ArCspA transgenic rice with/without cold stress. GO terms such as "response to stress (GO:0006950)", "response to cold (GO:0009409)", and "response to heat (GO:0009408)" were significantly enriched among the upregulated genes in the 35S:ArCspA transgenic rice under normal conditions, even without cold-stress treatment. The expression of five cold stress-related genes, Rab16B (Os11g0454200), Rab21 (Os11g0454300), LEA22 (Os01g0702500), ABI5 (Os01 g0859300), and MAPK5 (Os03g0285800), was significantly upregulated in the transgenic rice compared with the WT rice. These results indicate that the ArCspA gene might be involved in the induction of cold-responsive genes and provide cold tolerance.


Asunto(s)
Adaptación Fisiológica , Arthrobacter/metabolismo , Proteínas y Péptidos de Choque por Frío/fisiología , Frío , Oryza/fisiología , Microbiología del Suelo , Regiones Antárticas , Proteínas y Péptidos de Choque por Frío/aislamiento & purificación , Oryza/microbiología , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente
19.
Biology (Basel) ; 10(12)2021 Dec 03.
Artículo en Inglés | MEDLINE | ID: mdl-34943179

RESUMEN

Globally, the cultivation area of genetically modified (GM) crops is increasing dramatically. Despite their well-known benefits, they may also pose many risks to agriculture and the environment. Among the various GM crops, GM rapeseed (Brassica napus L.) is widely cultivated, mainly for oil production. At the same time, B. napus possesses a number of characteristics, including the ability to form feral populations and act as small-seeded weeds, and has a high potential for hybridization with other species. In this review, we provide an overview of the commercialization, approval status, and cultivation of GM rapeseed, as well as the status of the feral rapeseed populations. In addition, we highlight the case studies on the unintentional environmental release of GM rapeseed during transportation in several countries. Previous studies suggest that the main reason for the unintentional release is seed spillage during transport/importing of rapeseed in both GM rapeseed-cultivating and -non-cultivating countries. Despite the fact that incidents of unintentional release have been recorded often, there have been no reports of serious detrimental consequences. However, since rapeseed has a high potential for hybridization, the possibilities of gene flow within the genus, especially with B. rapa, are relatively significant, and considering their weedy properties, effective management methods are needed. Hence, we recommend that specific programs be used for the effective monitoring of environmental releases of GM rapeseed as well as management to avoid environmental and agricultural perturbations.

20.
Front Plant Sci ; 12: 767826, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-35095949

RESUMEN

GPD encodes glyceraldehyde-3-phosphate dehydrogenase enzyme involved in sugar mobilization, particularly glycolysis and gluconeogenesis. The objective of this study was to determine physiological aspects of germination and early seedling establishment of PsGPD (Pleurotus sajor-caju glyceraldehyde-3-phosphate dehydrogenase) expressing transgenic rice (T5) against different salt concentrations. The T5 line that carried 2 copies of T-DNA and had the highest level of PsGPD expression was used in the investigation. Final germination percentage, amylase activity, reducing sugar accumulation, and chlorophyll biosynthesis were comparatively higher in PsGPD expressing transgenic rice against elevating saline conditions. A slow-paced conversion of porphyrin's precursors was seen through the matrix model and further elaborated by a graphical model. A sustained level of porphyrin was observed in PsGPD expressing transgenic rice. These data were concurrent with the relative gene expression and thermal imaging (thermography) of PsGPD expressing transgenic rice against salt stress. Morphological attributes also favored the salt tolerance exhibited by PsGPD-transformed rice.

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