Low-level laser therapy prevents endothelial cells from TNF-α/cycloheximide-induced apoptosis.

Low-level laser therapy (LLLT), widely used in physiotherapy, has been known to enhance wound healing and stimulate cell proliferation, including fibroblast and endothelial cells. Applying LLLT can increase cell proliferation in many kinds of cells including fibroblasts and endothelial cells. However, the protective mechanisms of LLLT on endothelial apoptosis remain unclear. We hypothesized LLLT can protect endothelial cells from inflammation-induced apoptosis. Human endothelial cell line, EA.hy926 cells, and TNF-α/cycloheximide (TNF/CHX) were used to explore the protective effects of LLLT (660 nm) on inflammation-induced endothelial apoptosis. Cell viability, apoptosis, caspase-3/7/8/9 activity, MAPKs signaling, NF-κB activity, and inducible/endothelial nitric oxide synthase (iNOS/eNOS) expression were measured. Our results showed that LLLT increased EA.hy926 cell proliferation, attenuated the TNF/CHX-induced apoptosis, and reduced the TNF/CHX-mediated caspase-3/7/8/9 activation. In addition, LLLT increased ERK MAPK phosphorylation and suppressed the TNF/CHX-increased p38 MAPK, JNK, IKK phosphorylation, NF-κB translocation, and iNOS expression. The caspases-3 cleavage and cell death were not increased in cells treating with ERK inhibitor U0126, which implicated that ERK is not to be responsible for the protective effects of LLLT. After treating with p38 mitogen-activated protein kinase (MAPK) activator, the protection of LLLT in cell apoptosis was no longer existed, showing that LLLT protected the endothelial cells by suppressing p38 MAPK signaling. Our results provide a new insight into the possible molecular mechanisms in which LLLT protects against inflammatory-induced endothelial dysfunction.

Cingulum correlates of cognitive functions in patients with mild cognitive impairment and early Alzheimer's disease: a diffusion spectrum imaging study.

Diffusion spectrum imaging (DSI) of MRI can detect neural fiber tract changes. We investigated integrity of cingulum bundle (CB) in patients with mild cognitive impairment (MCI) and early Alzheimer's disease (EAD) using DSI tractography and explored its relationship with cognitive functions. We recruited 8 patients with MCI, 9 with EAD and 15 healthy controls (HC). All subjects received a battery of neuropsychological tests to access their executive, memory and language functions. We used a 3.0-tesla MRI scanner to obtain T1- and T2-weighted images for anatomy and used a pulsed gradient twice-refocused spin-echo diffusion echo-planar imaging sequence to acquire DSI. Patients with EAD performed significantly poorer than the HC on most tests in executive and memory functions. Significantly smaller general fractional anisotropy (GFA) values were found in the posterior and inferior segments of left CB and of the anterior segment of right CB of the EAD compared with those of the HC. Spearman's correlation on the patient groups showed that GFA values of the posterior segment of the left CB were significantly negatively associated with the time used to complete Color Trails Test Part II and positively correlated with performance of the logical memory and visual reproduction. GFA values of inferior segment of bilateral CB were positively associated with the performance of visual recognition. DSI tractography demonstrates significant preferential degeneration of the CB on the left side in patients with EAD. The location-specific degeneration is associated with corresponding declines in both executive and memory functions.

Consumer Visual and Affective Bias for Soothing Dolls.

Soothing dolls are becoming increasingly popular in a society with a lot of physical and mental stress. Many products are also combined with soothing dolls to stimulate consumers' desire for impulse buying. However, there is no research on the relationship between consumers' purchasing behavior, consumers' preference for soothing dolls, and visual preference. The purpose of this study was to examine the possible factors that affect the emotional and visual preferences of soothing dolls. Two local stores' sales lists were used to extract three different types of dolls. The 2D and 3D versions of these three dolls were used. Subjective emotional preferences were examined by the self-assessment manikin (SAM) scale, with 5-point Likert scales for valence and arousal factors. An eye tracker was used to examine visual preferences, both before and after positive/negative emotion stimulation by the International Affective Picture System (IAPS). There were 37 subjects involved, with an age range of 20-28 years. The experimental results show that the average valence/arousal scores for 2D/3D dolls were (3.80, 3.74) and (2.65, 2.68), respectively. There was no statistical difference, but both 2D and 3D pictures had high valence scores. Eye tracker analysis revealed no gaze difference in visual preference between 2D and 3D dolls. After negative emotional picture stimulation, the observation time of the left-side doll decreased from 2.307 (std 0.905) to 1.947 (std 1.038) seconds, p < 0.001; and that of the right-side picture increased from 1.898 (std 0.907) to 2.252 (std 1.046) seconds, p < 0.001. The average observation time ratio of the eye on the 3D doll was 40.6%, higher than that on the 2D doll (34.3%, p = 0.02). Soothing dolls may be beneficial for emotion relaxation. Soothing dolls always have high valence features according to the SAM evaluation's measurement. Moreover, this study proposes a novel research model using an eye-tracker and the SAM for the SOR framework.

Action Research on Applying Compound Stimulus Approach to Improve Empathetic Communication: The Case of Physical Therapy Students.

(1) Background: Empathetic communicative skills are the first step in establishing a good therapeutic relationship. The purpose of this study is to understand the effectiveness of improving the empathetic communicative skills applied to obtain accurate and precise information from patients via compound stimulus-drama in education. (2) Methods: A cross-sectional, one-group, pre- and post-test design was used for this study. In the two-day workshop, four clinical physiotherapists acted as tutors for the "Compound Stimulus-Drama in Education" module and assessed students' performances. The Standard Patient Rating Scale (SPRS), Objective Structured Clinical Examination Scale (OSCES), Professional and Communication Self-Assessment Scale (PCSS), Patients' Information (PI), and the Jefferson Scale of Empathy (JSE) were used to assess the students' empathy scores and communication skills, before and after the course. (3) Results: Fifty-seven students participated in this study. The results showed that there were significant improvements in the SPRS, OSCES, PCSS, PI, and JSE (p < 0.05). Both the quantitative data and the participants' reflection feedback suggest that this novel module was more helpful than traditional clinical practice courses for improving clinical empathy communication skills. (4) Conclusions: This study provided an innovative teaching model and assessment tools for learning clinic empathetic communicative skills in future education training.

The Impact of Online Learning on Physical and Mental Health in University Students during the COVID-19 Pandemic.

Higher education organizations have been influenced by the COVID-19 pandemic. During school closures, online teaching and learning has become a new routine that may lead to changes in lifestyles and adversely affect university students' health. Therefore, this study was to understand the potential impact of online learning on physical and mental health by investigating the differences in physical activity, psychological distress, and student life stress in Taiwanese university students between the in-class learning and online learning periods. A total of 181 students were recruited from a local university. All participants were requested to complete an online survey and self-report physical activity, psychological distress, and life stress in the in-class learning and online learning periods, respectively. The results indicated a significant reduction in physical activity of various intensities (p < 0.05). Specifically, male university students showed a greater decrease in vigorous physical activity compared to their female peers. Yet, there was no significant increase in psychological distress and life stress from the in-class learning period to the online learning period. In summary, physical activity drastically reduces during the online learning period in Taiwanese university students. Notably, male students may be at greater risk of insufficient participation in vigorous physical activity.

Cutoff Point of Mini-Balance Evaluation Systems Test Scores for Elderly Estimated by Center of Pressure Measurements by Linear Regression and Decision Tree Classification.

BACKGROUND: Understanding balance ability and assessing the risk of possible falls are very important for elderly rehabilitation. The Mini-Balanced Evaluation System Test (Mini-BESTest) is an important survey for older adults to evaluate subject balance, but it is not easy to complete due to various limitations of physical activities, including occasional fear of injury. A center of pressure (CoP) signal can be extracted from a force pressure plate with a short recording time, and it is relatively achievable to ask subjects to stand on a force pressure plate in a clinical environment. The goal of this study is to estimate the cutoff score of Mini-BESTest scores from CoP data. METHODS: CoP signals from a human balance evaluation database with data from 75 people were used. Time domain, frequency domain, and nonlinear domain parameters of 60 s CoP signals were extracted to classify different cutoff point scores for both linear regression and a decision tree algorithm. Classification performances were evaluated by accuracy and area under a receiver operating characteristic curve. RESULTS: The correlation coefficient between real and estimated Mini-BESTest scores by linear regression is 0.16. Instead of linear regression, binary classification accuracy above or below a cutoff point score was developed to examine the CoP classification performance for Mini-BESTest scores. The decision tree algorithm is superior to regression analysis among scores from 16 to 20. The highest area under the curve is 0.76 at a cutoff point score of 21 for the CoP measurement condition of eyes opened on the foam, and the corresponding classification accuracy is 76.15%. CONCLUSIONS: CoP measurement is a potential tool to estimate corresponding balance and fall survey scores for elderly rehabilitation and is useful for clinical users.

Meta-analysis of type and complexity of a secondary task during walking on the prediction of elderly falls.

AIM: Dual-tasking probes divided attention and causes performance changes that are associated with an increased risk for falls in the elderly. There is no systematic review investigating the effect of task type and complexity on the prediction of elderly falls. This article synthesizes research evidence regarding this issue on the contents of dual-tasking walking. METHODS: Relevant studies were systematically identified from electronic databases of Medline, PubMed, CINAHL, Cochrane CENTRAL and PsycINFO, and the reference lists of identified articles. The selection criteria were defined a priori. Two independent reviewers classified task types based on properties for cognitive demand, assessed the methodological quality with a customized checklist, and calculated the odds ratio of fall prediction. RESULTS: There was one study of reaction time, one of discrimination and decision-making, 10 of mental tracking, three of verbal fluency and five of manual tasks. The methodological heterogeneity was manifested in the selection criteria, faller classification, tasks and measures, resulting in substantial heterogeneity (I(2) 87-92%). Meta-analyses resulted in a significant pooled odds ratio 1.33 (95% CI 1.18-1.50). The mental tracking task was the only type that yielded a significant odds ratio 3.30 (95% CI 2.00-5.44). Running meta-analyses separately for simple and difficult mental tracking task showed similar odds ratios. CONCLUSION: The mental tracking task yielded significant dual-task-related changes for fall prediction. Most studies successively used an appropriate level of task complexity specific to the specified population of interest. More research is required for definite conclusions regarding the effect of task type and complexity.

A hierarchical categorisation of tasks in mobility disability.

PURPOSE: In the field of long-term care, disability usually refers to difficulties in instrumental activities of daily living (IADL) or basic activities of daily living (BADL); this term may also refer to difficulties in mobility for those more interested in preventive intervention or general health promotion. The aims of this study were to (1) categorise a complete set of mobility tasks according to a revealed hierarchy, and (2) examine the relationship between this mobility hierarchy and IADL/BADL disabilities. METHODS: We categorised nine mobility tasks according to appearance order in self-reported difficulties data obtained from a Taiwanese national database of community-dwelling elders aged over 65. We also performed correlation tests to explore the relationships of these mobility tasks with six tasks each of IADL and BADL. RESULTS: The results revealed a three-level hierarchy of mobility disability: (1) mild disability indicated by difficulties in four mobility tasks, which correlated with difficulty in one IADL task; (2) moderate disability indicated by difficulties in three mobility tasks, which correlated with difficulties in most IADL tasks; and (3) severe disability indicated by difficulties in two mobility tasks, which correlated with difficulties in all BADL tasks. The same hierarchy was observed for males and females. CONCLUSIONS: There is a clear hierarchical structure of mobility disability that correlates differently with IADL and BADL disabilities. These results suggest that different mobility tasks should be included in disability assessments to suit specific purposes.

Altered muscle activation characteristics associated with single volitional forward stepping in middle-aged adults.

BACKGROUND: Middle-aged adults show a higher incidence of falls compared to young adults when performing outdoor physical activities. This study investigated whether or not the patterns and quantitative characteristics of the trunk and lower extremity muscle activations associated with stepping, which represents an important movement for arresting falls, differ between middle-aged adults and young adults. METHODS: Nine healthy young adults (age=22[3] years) and nine healthy middle-aged adults (age=52[8] years), performed a single-step, volitional, fast forward stepping movement with each leg. The stepping movement was divided into the step-initiation, single-leg-support, and landing phases based on foot-switch signals. The activation sequence, occurrence rate, onset latency, burst duration, and normalized co-contraction duration of the tibialis anterior, medial gastrocnemius, rectus femoris, biceps femoris, and gluteus medius of the stance and swing legs and that of bilateral erector spinae muscles were analyzed using surface electromyography. We defined the essential muscle activation as exhibiting an occurrence rate of 90% or more in all of these trials. FINDINGS: As compared to young adults, the middle-aged adults demonstrated several additional essential bursts throughout the stepping movement. Middle-aged adults also displayed significantly longer burst durations of the biceps femoris and medial gastrocnemius of the swing leg after landing, as well as longer co-contraction of the rectus femoris and biceps femoris of the stance leg in the single-leg-support phase (P<0.05). INTERPRETATION: Age-related changes in step-related neuromuscular control exist in healthy middle-aged adults. We propose that training focused on improving or maintaining neuromuscular control associated with volitional leg movements may benefit middle-aged individuals.