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1.
Am J Transplant ; 24(1): 30-36, 2024 01.
Artículo en Inglés | MEDLINE | ID: mdl-37633449

RESUMEN

De novo membranous nephropathy (dnMN) is an uncommon immune complex-mediated late complication of human kidney allografts that causes proteinuria. We report here the first case of dnMN in a pig-to-baboon kidney xenograft. The donor was a double knockout (GGTA1 and ß4GalNT1) genetically engineered pig with a knockout of the growth hormone receptor and addition of 6 human transgenes (hCD46, hCD55, hTBM, hEPCR, hHO1, and hCD47). The recipient developed proteinuria at 42 days posttransplant, which progressively rose to the nephrotic-range at 106 days, associated with an increase in serum antidonor IgG. Kidney biopsies showed antibody-mediated rejection (AMR) with C4d and thrombotic microangiopathy that eventually led to graft failure at 120 days. In addition to AMR, the xenograft had diffuse, global granular deposition of C4d and IgG along the glomerular basement membrane on days 111 and 120. Electron microscopy showed extensive amorphous subepithelial electron-dense deposits with intervening spikes along the glomerular basement membrane. These findings, in analogy to human renal allografts, are interpreted as dnMN in the xenograft superimposed on AMR. The target was not identified but is hypothesized to be a pig xenoantigen expressed on podocytes. Whether dnMN will be a significant problem in other longer-term xenokidneys remains to be determined.


Asunto(s)
Glomerulonefritis Membranosa , Enfermedades Renales , Trasplante de Riñón , Humanos , Porcinos , Animales , Glomerulonefritis Membranosa/etiología , Trasplante de Riñón/efectos adversos , Xenoinjertos , Riñón/patología , Enfermedades Renales/patología , Proteinuria/etiología , Inmunoglobulina G , Rechazo de Injerto/patología
2.
Am J Transplant ; 24(6): 1016-1026, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38341027

RESUMEN

Membranous nephropathy (MN) is a leading cause of kidney failure worldwide and frequently recurs after transplant. Available data originated from small retrospective cohort studies or registry analyses; therefore, uncertainties remain on risk factors for MN recurrence and response to therapy. Within the Post-Transplant Glomerular Disease Consortium, we conducted a retrospective multicenter cohort study examining the MN recurrence rate, risk factors, and response to treatment. This study screened 22,921 patients across 3 continents and included 194 patients who underwent a kidney transplant due to biopsy-proven MN. The cumulative incidence of MN recurrence was 31% at 10 years posttransplant. Patients with a faster progression toward end-stage kidney disease were at higher risk of developing recurrent MN (hazard ratio [HR], 0.55 per decade; 95% confidence interval [CI], 0.35-0.88). Moreover, elevated pretransplant levels of anti-phospholipase A2 receptor (PLA2R) antibodies were strongly associated with recurrence (HR, 18.58; 95% CI, 5.37-64.27). Patients receiving rituximab for MN recurrence had a higher likelihood of achieving remission than patients receiving renin-angiotensin-aldosterone system inhibition alone. In sum, MN recurs in one-third of patients posttransplant, and measurement of serum anti-PLA2R antibody levels shortly before transplant could aid in risk-stratifying patients for MN recurrence. Moreover, patients receiving rituximab had a higher rate of treatment response.


Asunto(s)
Glomerulonefritis Membranosa , Trasplante de Riñón , Recurrencia , Humanos , Glomerulonefritis Membranosa/etiología , Glomerulonefritis Membranosa/patología , Glomerulonefritis Membranosa/tratamiento farmacológico , Trasplante de Riñón/efectos adversos , Masculino , Estudios Retrospectivos , Femenino , Persona de Mediana Edad , Factores de Riesgo , Estudios de Seguimiento , Pronóstico , Adulto , Tasa de Filtración Glomerular , Fallo Renal Crónico/cirugía , Complicaciones Posoperatorias , Supervivencia de Injerto , Pruebas de Función Renal , Incidencia , Rechazo de Injerto/etiología , Rechazo de Injerto/patología , Tasa de Supervivencia
3.
J Am Soc Nephrol ; 33(1): 238-252, 2022 01.
Artículo en Inglés | MEDLINE | ID: mdl-34732507

RESUMEN

BACKGROUND: Failure of the glomerular filtration barrier, primarily by loss of slit diaphragm architecture, underlies nephrotic syndrome in minimal change disease. The etiology remains unknown. The efficacy of B cell-targeted therapies in some patients, together with the known proteinuric effect of anti-nephrin antibodies in rodent models, prompted us to hypothesize that nephrin autoantibodies may be present in patients with minimal change disease. METHODS: We evaluated sera from patients with minimal change disease, enrolled in the Nephrotic Syndrome Study Network (NEPTUNE) cohort and from our own institutions, for circulating nephrin autoantibodies by indirect ELISA and by immunoprecipitation of full-length nephrin from human glomerular extract or a recombinant purified extracellular domain of human nephrin. We also evaluated renal biopsies from our institutions for podocyte-associated punctate IgG colocalizing with nephrin by immunofluorescence. RESULTS: In two independent patient cohorts, we identified circulating nephrin autoantibodies during active disease that were significantly reduced or absent during treatment response in a subset of patients with minimal change disease. We correlated the presence of these autoantibodies with podocyte-associated punctate IgG in renal biopsies from our institutions. We also identified a patient with steroid-dependent childhood minimal change disease that progressed to end stage kidney disease; she developed a massive post-transplant recurrence of proteinuria that was associated with high pretransplant circulating nephrin autoantibodies. CONCLUSIONS: Our discovery of nephrin autoantibodies in a subset of adults and children with minimal change disease aligns with published animal studies and provides further support for an autoimmune etiology. We propose a new molecular classification of nephrin autoantibody minimal change disease to serve as a framework for instigation of precision therapeutics for these patients.


Asunto(s)
Autoanticuerpos/sangre , Proteínas de la Membrana/inmunología , Nefrosis Lipoidea/sangre , Nefrosis Lipoidea/etiología , Adulto , Niño , Preescolar , Estudios de Cohortes , Femenino , Humanos , Masculino , Nefrosis Lipoidea/patología , Podocitos/patología
4.
Am J Kidney Dis ; 78(6): 793-803, 2021 12.
Artículo en Inglés | MEDLINE | ID: mdl-34174365

RESUMEN

RATIONALE & OBJECTIVE: B-cell depletion with rituximab has emerged as a first-line therapy for primary membranous nephropathy (MN). However, most patients do not achieve complete remission with rituximab monotherapy. In this case series, we report longer-term remission and relapse rates, anti-phospholipase A2 receptor (PLA2R) antibody levels, B-cell levels, and serious adverse events in patients with primary MN who received rituximab combined with an initial short course of low-dose oral cyclophosphamide and a course of rapidly tapered prednisone. STUDY DESIGN: Single-center retrospective case series. SETTING & PARTICIPANTS: 60 consecutive patients with primary MN treated with the combination of rituximab, low-dose cyclophosphamide, and prednisone at the Vasculitis and Glomerulonephritis Center at the Massachusetts General Hospital. FINDINGS: After treatment initiation, median follow-up was 38 (interquartile range [IQR], 25-62) months; 100% of patients achieved partial remission, defined as a urinary protein-creatinine ratio (UPCR) < 3 g/g and a 50% reduction from baseline, at a median of 3.4 months. By 2 years after treatment initiation, 83% achieved complete remission, defined as a UPCR < 0.3 g/g. The median time to complete remission was 12.4 months. Immunologic remission (defined by an anti-PLA2R titer < 14 RU/mL) was achieved by 86% and 100% of anti-PLA2R seropositive patients (n = 29) at 3 and 6 months, respectively, after treatment initiation. After 1 year, the median UPCR fell from 8.4 (IQR, 5.0-10.7) to 0.3 (IQR, 0.2-0.8) g/g (P < 0.001). No patient relapsed throughout the duration of B-cell depletion. Relapse occurred in 10% of patients at 2 years after the onset of B-cell reconstitution following the last rituximab dose. Over a combined follow-up time of 228 patient-years, 18 serious adverse events occurred. One death occurred unrelated to treatment or primary MN, and 1 patient progressed to kidney failure requiring kidney replacement therapy. LIMITATIONS: Absence of a comparison group. CONCLUSIONS: All patients with primary MN treated with combination therapy achieved partial remission and most achieved a durable complete remission with an acceptable safety profile.


Asunto(s)
Glomerulonefritis Membranosa , Ciclofosfamida/efectos adversos , Estudios de Seguimiento , Glomerulonefritis Membranosa/tratamiento farmacológico , Humanos , Inmunosupresores , Prednisona , Receptores de Fosfolipasa A2 , Estudios Retrospectivos , Rituximab , Resultado del Tratamiento
5.
J Am Soc Nephrol ; 29(2): 644-653, 2018 02.
Artículo en Inglés | MEDLINE | ID: mdl-29074737

RESUMEN

Primary renal tubulointerstitial disease resulting from proximal tubule antigen-specific antibodies and immune complex formation has not been well characterized in humans. We report a cohort of patients with a distinct, underappreciated kidney disease characterized by kidney antibrush border antibodies and renal failure (ABBA disease). We identified ten patients with ABBA disease who had a combination of proximal tubule damage, IgG-positive immune deposits in the tubular basement membrane, and circulating antibodies reactive with normal human kidney proximal tubular brush border. All but one of the patients also had segmental glomerular deposits on renal biopsy specimen. Patients with ABBA disease were elderly and presented with AKI and subnephrotic proteinuria. Serum from all patients but not controls recognized a high molecular weight protein in renal tubular protein extracts that we identified as LDL receptor-related protein 2 (LRP2), also known as megalin, by immunoprecipitation and mass spectrometry. Immunostaining revealed that LRP2 specifically colocalized with IgG in the tubular immune deposits on the ABBA biopsy specimen but not the control specimen analyzed. Finally, ABBA serum samples but not control samples showed reactivity against recombinantly expressed N-terminal LRP2 fragments on Western blots and immunoprecipitated the recombinantly expressed N-terminal region of LRP2. This case series details the clinicopathologic findings of patients with ABBA disease and shows that the antigenic target of these autoantibodies is LRP2. Future studies are needed to determine the disease prevalence, stimulus for ABBA, and optimal treatment.


Asunto(s)
Autoanticuerpos/sangre , Túbulos Renales Proximales/inmunología , Proteína 2 Relacionada con Receptor de Lipoproteína de Baja Densidad/inmunología , Nefritis Intersticial/inmunología , Lesión Renal Aguda/inmunología , Anciano , Anciano de 80 o más Años , Membrana Basal/metabolismo , Femenino , Humanos , Inmunoglobulina G/metabolismo , Túbulos Renales Proximales/metabolismo , Túbulos Renales Proximales/patología , Proteína 2 Relacionada con Receptor de Lipoproteína de Baja Densidad/metabolismo , Masculino , Microvellosidades/inmunología , Nefritis Intersticial/metabolismo , Nefritis Intersticial/patología
6.
J Am Soc Nephrol ; 27(2): 380-4, 2016 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-26334028

RESUMEN

Immune complex tubulointerstitial nephritis due to antibodies to brush border antigens of the proximal tubule has been demonstrated experimentally and rarely in humans. Our patient developed ESRD and early recurrence after transplantation. IgG and C3 deposits were conspicuous in the tubular basement membrane of proximal tubules, corresponding to deposits observed by electron microscopy. Rare subepithelial deposits were found in the glomeruli. The patient had no evidence of SLE and had normal complement levels. Serum samples from the patient reacted with the brush border of normal human kidney, in contrast with the negative results with 20 control serum samples. Preliminary characterization of the brush border target antigen excluded megalin, CD10, and maltase. We postulate that antibodies to brush border antigens cause direct epithelial injury, accumulate in the tubular basement membrane, and elicit an interstitial inflammatory response.


Asunto(s)
Complejo Antígeno-Anticuerpo , Autoanticuerpos/inmunología , Túbulos Renales Proximales/inmunología , Nefritis Intersticial/inmunología , Anciano , Biopsia , Estudios de Seguimiento , Humanos , Túbulos Renales Proximales/patología , Masculino , Nefritis Intersticial/patología
7.
Kidney Int ; 89(4): 897-908, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26994577

RESUMEN

Classic anti-glomerular basement membrane (GBM) disease presents with rapidly progressive glomerulonephritis (GN) with or without pulmonary hemorrhage. On biopsy typical disease displays bright polytypic linear GBM staining for IgG by immunofluorescence and diffuse crescentic/necrotizing GN on light microscopy. Here, we studied 20 patients with atypical anti-GBM nephritis typified by bright linear GBM staining for immunoglobulins but without a diffuse crescentic phenotype. Patients had hematuria, proteinuria, and mild renal insufficiency, without pulmonary hemorrhage. Light microscopy showed endocapillary proliferative GN in 9 patients, mesangial proliferative GN in 6, membranoproliferative GN in 3, and focal segmental glomerulosclerosis with mesangial hypercellularity in 2. Eight of the 20 showed features of microangiopathy. Crescents/necrosis were absent in 12 and were focal in 8 patients. Bright linear GBM staining for IgG was seen in 17 patients, IgM in 2, and IgA in 1 patient, which was polytypic in 10 patients and monotypic in 10 patients. No circulating α3NC1 antibodies were detected by commercial ELISA. The 1-year patient and renal survival rates were 93% and 85%, respectively. Thus, atypical anti-GBM nephritis is a rare variant of anti-GBM disease characterized clinically by an indolent course, no pulmonary involvement, and undetectable circulating α3NC1 antibodies. Further studies are needed to characterize the molecular architecture of GBM autoantigens in these patients and establish optimal therapy.


Asunto(s)
Enfermedad por Anticuerpos Antimembrana Basal Glomerular/patología , Riñón/patología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Biopsia , Femenino , Técnica del Anticuerpo Fluorescente , Humanos , Riñón/ultraestructura , Masculino , Persona de Mediana Edad , Adulto Joven
8.
Am J Kidney Dis ; 64(6): 987-93, 2014 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25304985

RESUMEN

Glomerulopathy is an uncommon but increasingly recognized complication of hematopoietic cell transplantation. It typically manifests as membranous nephropathy, less commonly as minimal change disease, and rarely as proliferative glomerulonephritis. There is evidence to suggest that these glomerulopathies might represent manifestations of chronic graft-versus-host disease. In this report, we focus on membranous nephropathy as the most common form of glomerulopathy after hematopoietic cell transplantation. We present a case of membranous nephropathy that developed 483 days post-allogeneic hematopoietic stem cell transplantation in a patient with a history of acute graft-versus-host disease. We also share our experience with 4 other cases of membranous nephropathy occurring after allogeneic hematopoietic stem cell transplantation. Clinicopathologic correlates, including the association with graft-versus-host-disease, HLA antigen typing, glomerular deposition of immunoglobulin G (IgG) subclasses, subepithelial colocalization of IgG deposits with phospholipase A2 receptor staining, C4d deposition along the peritubular capillaries, and treatment, are discussed with references to the literature.


Asunto(s)
Complemento C4b , Glomerulonefritis Membranosa/diagnóstico , Enfermedad Injerto contra Huésped/diagnóstico , Antígenos HLA , Fragmentos de Péptidos , Receptores de Fosfolipasa A2 , Adulto , Diagnóstico Diferencial , Estudios de Seguimiento , Glomerulonefritis Membranosa/sangre , Glomerulonefritis Membranosa/complicaciones , Enfermedad Injerto contra Huésped/sangre , Enfermedad Injerto contra Huésped/complicaciones , Antígenos HLA/sangre , Trasplante de Células Madre Hematopoyéticas/efectos adversos , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina G/química , Masculino , Fragmentos de Péptidos/sangre , Estructura Secundaria de Proteína , Receptores de Fosfolipasa A2/sangre
9.
J Am Soc Nephrol ; 22(1): 176-86, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21115619

RESUMEN

Interstitial fibrosis is an outcome measure of increasing importance in clinical trials of both renal transplantation and native disease, but data on the comparative advantages of fibrosis measurement methods are limited. We compared four morphometric techniques and contrasted these with two visual fibrosis-scoring methods on trichrome-stained slides. Two morphometric methods included whole-slide digital images: collagen III immunohistochemistry and a new technique using trichrome and periodic acid-Schiff subtraction morphometry; the other two methods included Sirius Red with and without polarization on multiple digital fields. We evaluated 10 serial sections from 15 renal biopsies with a range of fibrosis extent and diagnoses on duplicate sections with each method on separate days. Three pathologists performed visual scoring on whole-slide images. Visual and morphometric techniques had good to excellent interassay reproducibility (R(2) = 0.62 to 0.96) and interobserver reproducibility (R(2) = 0.75 to 0.99, all P < 0.001). Morphometry showed less variation between observers than visual assessment (mean of 1% to 5% versus 11% to 13%). Collagen III, Sirius Red unpolarized, and visual scores had the strongest correlations (R(2) = 0.78 to 0.89), the greatest dynamic range, and the best correlation with estimated GFR (R(2) = 0.38 to 0.50, P < 0.01 to 0.001). Considering efficiency, reproducibility, and functional correlation, two current techniques stand out as potentially the best for clinical trials: collagen III morphometry and visual assessment of trichrome-stained slides.


Asunto(s)
Histocitoquímica/métodos , Inmunohistoquímica/métodos , Riñón/patología , Adolescente , Adulto , Compuestos Azo , Biopsia , Niño , Preescolar , Colágeno Tipo III/metabolismo , Eosina Amarillenta-(YS) , Fibrosis , Humanos , Riñón/metabolismo , Verde de Metilo , Persona de Mediana Edad , Reacción del Ácido Peryódico de Schiff , Reproducibilidad de los Resultados , Adulto Joven
10.
Kidney Int ; 80(8): 879-85, 2011 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-21697808

RESUMEN

Transplant glomerulopathy (TG) has received much attention in recent years as a symptom of chronic humoral rejection; however, many cases lack C4d deposition and/or circulating donor-specific antibodies (DSAs). To determine the contribution of other causes, we studied 209 consecutive renal allograft indication biopsies for chronic allograft dysfunction, of which 25 met the pathological criteria of TG. Three partially overlapping etiologies accounted for 21 (84%) cases: C4d-positive (48%), hepatitis C-positive (36%), and thrombotic microangiopathy (TMA)-positive (32%) TG. The majority of patients with confirmed TMA were also hepatitis C positive, and the majority of hepatitis C-positive patients had TMA. DSAs were significantly associated with C4d-positive but not with hepatitis C-positive TG. The prevalence of hepatitis C was significantly higher in the TG group than in 29 control patients. Within the TG cohort, those who were hepatitis C-positive developed allograft failure significantly earlier than hepatitis C-negative patients. Thus, TG is not a specific diagnosis but a pattern of pathological injury involving three major overlapping pathways. It is important to distinguish these mechanisms, as they may have different prognostic and therapeutic implications.


Asunto(s)
Rechazo de Injerto/complicaciones , Hepatitis C/complicaciones , Enfermedades Renales/etiología , Glomérulos Renales/patología , Trasplante de Riñón/efectos adversos , Microangiopatías Trombóticas/complicaciones , Enfermedad Crónica , Complemento C4b , Humanos , Enfermedades Renales/patología , Análisis Multivariante , Fragmentos de Péptidos/sangre , Donantes de Tejidos
11.
Kidney Int Suppl ; (119): S13-7, 2010 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-21116310

RESUMEN

B cells have many possible mechanisms by which they can affect allograft survival, including antigen presentation, cytokine production, immune regulation, and differentiation into alloantibody-producing plasma cells. This report reviews the last mechanism, which the authors regard as most critical for the long-term survival of allografts, namely, the promotion of chronic rejection by alloantibodies. Chronic humoral rejection characteristically arises late after transplantation and causes transplant glomerulopathy, multilamination of peritubular capillary basement membranes, and C4d deposition in PTCs and glomeruli. Circulating antidonor human leukocyte antigen class II antibodies are commonly detected and may precede the development of graft injury. Prognosis is poor, especially when recognized after graft dysfunction has developed. Improved detection and treatment are critically needed for this common cause of late graft loss.


Asunto(s)
Linfocitos B/inmunología , Rechazo de Injerto/inmunología , Enfermedades Renales/inmunología , Trasplante de Riñón/efectos adversos , Riñón/inmunología , Animales , Enfermedad Crónica , Rechazo de Injerto/prevención & control , Humanos , Inmunidad Humoral , Terapia de Inmunosupresión/métodos , Isoanticuerpos/biosíntesis , Isoantígenos/inmunología , Riñón/fisiopatología , Enfermedades Renales/fisiopatología , Enfermedades Renales/prevención & control , Factores de Tiempo , Trasplante Homólogo , Resultado del Tratamiento
12.
J Endocrinol Invest ; 31(1): 57-61, 2008 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-18296906

RESUMEN

Mice lacking the LDL receptor associated protein (RAP) have a severe defect of thyroglobulin secretion into the colloid, associated with moderately increased serum TSH levels and histological features of early goiter. RAP is expressed also in renal proximal tubule cells, where it functions as a molecular chaperone for the endocytic receptor megalin, which is responsible for reabsorption of low molecular weight proteins from the glomerular filtrate. Here we investigated whether the thyroid phenotype in RAP knockout (KO) mice is associated with kidney alterations. By immunohistochemistry, we found that in RAP KO mice megalin expression on the apical membrane of renal proximal tubule cells was markedly reduced, with intracellular retention of the receptor. The reduced expression of megalin was associated with its impaired function. Thus, urinary protein concentrations and urinary protein excretion in 24 h were higher in RAP KO than in wild-type mice. Coomassie staining of urine samples revealed an increased intensity of low molecular mass bands in the urine of RAP KO mice, indicating that they had low molecular weight proteinuria. Therefore, we concluded that disruption of the RAP gene determines not only thyroid abnormalities, but also a severe defect of megalin expression and function in the kidney.


Asunto(s)
Riñón/anomalías , Proteína Asociada a Proteínas Relacionadas con Receptor de LDL/genética , Animales , Riñón/metabolismo , Riñón/fisiología , Proteína 2 Relacionada con Receptor de Lipoproteína de Baja Densidad/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Glándula Tiroides/anomalías
13.
Hum Pathol ; 72: 71-79, 2018 02.
Artículo en Inglés | MEDLINE | ID: mdl-29133141

RESUMEN

In the past, the diagnosis and typing of amyloidosis often required an invasive biopsy of an internal organ, such as the heart or kidneys. Abdominal fat pad excisional biopsy (FPEB) offers a less invasive approach, but the sensitivity of this technique has been unclear. To determine the sensitivity of FPEB for immunoglobulin light chain (AL) and transthyretin (ATTR) amyloidosis, we performed a retrospective clinicopathologic analysis of 97 patients who had undergone FPEB, of which 16 were positive for amyloid. The most significant pretest feature predicting a positive FPEB was a serum free light chain κ/λ ratio less than .5, and in this group of patients the probability of a positive biopsy was dependent on the size of the biopsy (P=.004). In FPEBs, the amyloid was present in multiple distinct patterns: pericellular, septal, medium-sized vessel, small vessel, and nodular. For patients with AL amyloidosis for which direct typing was attempted using the FPEB tissue, the amyloid was successfully typed in the FPEB in 90% of cases. The overall sensitivity of FPEB was 79% for AL amyloidosis and 12% for ATTR amyloidosis (P=.0003). In patients with AL amyloidosis, the sensitivity of FPEB was dependent on biopsy size, with small biopsies (≤700 mm3) having a sensitivity of ~50%, and large biopsies (>700 mm3) having a sensitivity of ~100%. This study demonstrates that FPEB has high sensitivity for AL amyloidosis, and can be routinely used to type the amyloid. However, FPEB has low sensitivity for ATTR amyloidosis in our patient population.


Asunto(s)
Tejido Adiposo/patología , Amiloidosis/patología , Lipomatosis/patología , Mieloma Múltiple/patología , Adulto , Anciano , Anciano de 80 o más Años , Amiloide/metabolismo , Amiloidosis/diagnóstico , Biopsia , Femenino , Humanos , Riñón/patología , Lipomatosis/diagnóstico , Masculino , Persona de Mediana Edad , Mieloma Múltiple/diagnóstico , Estudios Retrospectivos
14.
Am J Surg Pathol ; 31(10): 1586-97, 2007 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-17895762

RESUMEN

Autoimmune pancreatitis (AIP) is a mass-forming chronic fibroinflammatory condition centered on the pancreatobiliary system and characterized by predominant immunoglobulin G4 (IgG4)-positive plasma cells. Recent reports have brought to light the multiorgan involvement of this disease. We describe a series of 5 cases of tubulointerstitial nephritis (TIN) associated with AIP and characterize the clinical, pathologic, ultrastructural, and immunopathologic features of TIN. The specimens consisted of 4 biopsies and 1 nephrectomy. The average patient age was 64 years (range 45 to 78) and the male to female ratio was 4:1. All had histologic and/or clinical and radiographic evidence of AIP, mass-forming sclerosing cholangitis, or both. The clinical impression in 4 patients was a renal mass or vasculitis. Two patients had renal insufficiency. Histologic preparations revealed a dense tubulointerstitial lymphoplasmacytic infiltrate. Eosinophils were often numerous. Tubulitis and tubular injury were present, along with tubular atrophy with focally thickened tubular basement membranes (TBMs). The histologic appearance ranged from a cellular, inflammatory pattern without tubular atrophy to a striking expansive interstitial fibrosis with tubular destruction. The nephrectomy specimen demonstrated a masslike nodular pattern of inflammation with normal renal tissue elsewhere. Glomeruli were uninvolved. By immunohistochemistry or immunofluorescence, numerous plasma cells in the infiltrate were positive for IgG4. TBM granular IgG deposits, predominantly of the IgG4 subclass, were detected in 4 of 5 cases by either immunofluorescence or immunohistochemistry. By electron microscopy, corresponding amorphous electron-dense deposits were present in the TBM in these cases. This type of TIN, typically characterized by a masslike lesion consisting of a lymphoplasmacytic infiltrate with eosinophils and prominent IgG4-positive plasma cells and immune-complex deposits in the TBM, may be part of a systemic IgG4-related disease, which we term "IgG4-associated immune complex Multiorgan Autoimmune Disease" (IMAD).


Asunto(s)
Enfermedades Autoinmunes/patología , Granuloma de Células Plasmáticas/patología , Inmunoglobulina G/análisis , Nefritis Intersticial/patología , Pancreatitis/patología , Anciano , Complejo Antígeno-Anticuerpo/inmunología , Enfermedades Autoinmunes/complicaciones , Enfermedades Autoinmunes/inmunología , Membrana Basal/ultraestructura , Femenino , Granuloma de Células Plasmáticas/complicaciones , Granuloma de Células Plasmáticas/inmunología , Humanos , Técnicas para Inmunoenzimas , Túbulos Renales/inmunología , Túbulos Renales/patología , Masculino , Persona de Mediana Edad , Nefritis Intersticial/complicaciones , Nefritis Intersticial/inmunología , Pancreatitis/complicaciones , Pancreatitis/inmunología , Células Plasmáticas/inmunología , Células Plasmáticas/patología , Células Plasmáticas/ultraestructura
15.
J Cardiovasc Transl Res ; 8(4): 264-8, 2015 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-25925232

RESUMEN

Accurate and rapid classification of cardiac amyloidosis is important for patient management. We have optimized the use of serum free light chain kappa and lambda values to differentiate immunoglobulin light chain amyloid (AL) amyloidosis from transthyretin amyloid and amyloid A using 85 cases of tissue-proven cardiac amyloidosis, in which there was direct classification of amyloidosis by mass spectrometry or immunofluorescence. The serum free light chain kappa/lambda ratios were non-overlapping for the three major groups: AL-lambda (0.01-0.41, n = 30), non-AL (0.52-2.7, n = 43), and AL-kappa (6.7-967, n = 12). A kappa/lambda ratio value between 0.5 and 5.0 had 100 % sensitivity and 100 % specificity for distinguishing AL amyloidosis from non-AL amyloidosis. This optimized range for serum light chain kappa/lambda ratio provides extremely robust classification of cardiac amyloidosis. Cases of cardiac amyloidosis in which the serum kappa/lambda free light chain ratio falls close to these new cutoff values may benefit most from direct amyloid subtyping.


Asunto(s)
Amiloidosis/sangre , Cardiomiopatías/sangre , Cadenas kappa de Inmunoglobulina/sangre , Anciano , Anciano de 80 o más Años , Neuropatías Amiloides Familiares/sangre , Neuropatías Amiloides Familiares/diagnóstico , Neuropatías Amiloides Familiares/inmunología , Amiloidosis/clasificación , Amiloidosis/diagnóstico , Amiloidosis/inmunología , Baltimore , Biomarcadores/sangre , Boston , Cardiomiopatías/clasificación , Cardiomiopatías/diagnóstico , Cardiomiopatías/inmunología , Diagnóstico Diferencial , Femenino , Humanos , Cadenas lambda de Inmunoglobulina , Masculino , Persona de Mediana Edad , Miocardio/inmunología , Valor Predictivo de las Pruebas , Proteína Amiloide A Sérica/análisis
17.
Am J Clin Pathol ; 137(1): 51-64, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22180478

RESUMEN

Extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT) lymphoma is a mature B-cell neoplasm that typically follows an indolent clinical course. Amyloid deposition associated with MALT lymphoma is uncommon. We describe the clinical and pathologic features of 20 cases of MALT lymphoma and associated amyloid deposition across diverse primary sites. Frozen section immunofluorescence performed on 4 cases suggests that these deposits are a localized form of AL amyloid. Clinical follow-up was available for 15 patients. Amyloid deposits distant from the initial site occurred in 5 cases, always at sites also involved by the underlying lymphoma. No definitive evidence of systemic amyloidosis affecting the heart, kidneys, or liver was present in any patient. Given the generally indolent clinical behavior of MALT lymphomas with associated amyloid, we do not recommend extensive follow-up testing for systemic amyloidosis or more aggressive therapy than would be indicated for other MALT lymphomas of similar clinical stage.


Asunto(s)
Amiloide/metabolismo , Amiloidosis/patología , Linfoma de Células B de la Zona Marginal/patología , Adulto , Anciano , Anciano de 80 o más Años , Amiloidosis/complicaciones , Amiloidosis/metabolismo , Femenino , Estudios de Seguimiento , Humanos , Inmunofenotipificación , Linfoma de Células B de la Zona Marginal/complicaciones , Linfoma de Células B de la Zona Marginal/metabolismo , Masculino , Persona de Mediana Edad
18.
Tex Heart Inst J ; 39(1): 71-5, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22412233

RESUMEN

Multiple precursor proteins have been shown to cause cardiac amyloidosis. The most common forms are due either to immunoglobulin light chains or to transthyretin proteins (either wild-type or mutant forms). Correct subclassification of the amyloid is paramount because treatment differs in accordance with the type of amyloidosis. Indirect diagnostic methods, including serologic analysis, can lead to misdiagnosis. Definitive diagnosis often requires analysis of amyloid in the tissue. We present a case of a woman who was diagnosed with hereditary transthyretin cardiac amyloidosis by means of immunofluorescence and genetic analysis. This case highlights the importance-in the diagnostic algorithm of cardiac amyloidosis-of direct evaluation of the tissue with immunofluorescence and of genetic testing.


Asunto(s)
Amiloide/química , Amiloidosis Familiar/diagnóstico , Cardiomiopatías/diagnóstico , Análisis Mutacional de ADN , Técnica del Anticuerpo Fluorescente , Mutación , Miocardio/química , Prealbúmina , Anciano , Amiloidosis Familiar/genética , Amiloidosis Familiar/metabolismo , Amiloidosis Familiar/terapia , Biopsia , Cardiomiopatías/genética , Cardiomiopatías/metabolismo , Cardiomiopatías/terapia , Ecocardiografía , Femenino , Humanos , Imagen por Resonancia Magnética , Miocardio/patología , Prealbúmina/análisis , Prealbúmina/genética , Valor Predictivo de las Pruebas
19.
Hum Pathol ; 42(5): 734-7, 2011 May.
Artículo en Inglés | MEDLINE | ID: mdl-21236468

RESUMEN

Tongue necrosis is a rare but dramatic clinical finding, which is most often related to infection, trauma, malignancy, or vasculitis. We describe an extremely unusual case in which systemic amyloid deposition in small arteries caused by senile systemic amyloidosis was associated with necrosis of the tongue as well as necrosis of portions of the bowel. To our knowledge, this is the first report of ischemic tongue necrosis resulting from vascular amyloidosis.


Asunto(s)
Amiloidosis/patología , Lengua/patología , Enfermedades Vasculares/patología , Anciano de 80 o más Años , Femenino , Humanos , Intestinos/patología , Miocardio/patología , Necrosis
20.
Cardiovasc Pathol ; 18(4): 205-16, 2009.
Artículo en Inglés | MEDLINE | ID: mdl-18619857

RESUMEN

BACKGROUND: At least 12 distinct forms of amyloidosis are known to involve the heart or great vessels. Patient treatment regimens require proper subtyping of amyloid deposits in small diagnostic cardiac specimens. A growing lack of confidence in immunohistochemical staining for subtyping amyloid has arisen primarily as a result of studies utilizing immunoperoxidase staining of formalin-fixed paraffin-embedded tissue. Immunofluorescence staining on fresh frozen tissue is generally considered superior to immunoperoxidase staining for subtyping amyloid; however, this technique has not previously been reported in a series of cardiac specimens. METHODS: Amyloid deposits were subtyped in 17 cardiac specimens and 23 renal specimens using an immunofluorescence panel. RESULTS: Amyloid deposits were successfully subtyped as AL, AH, or AA amyloid by immunofluorescence in 82% of cardiac specimens and 87% of renal specimens. In all cases, the amyloid classification was in good agreement with available clinical and laboratory assessments. A cross-study analysis of 163 cases of AL amyloidosis reveals probable systemic misdiagnosis of cardiac AL amyloidosis by the immunoperoxidase technique, but not by the immunofluorescence technique. CONCLUSIONS: Amyloid deposits can be reliably subtyped in small diagnostic cardiac specimens using immunofluorescence. The practical aspects of implementing an immunofluorescence approach are compared with those of other approaches for subtyping amyloid in the clinical setting.


Asunto(s)
Amiloide/análisis , Amiloidosis/diagnóstico , Técnica del Anticuerpo Fluorescente , Cardiopatías/diagnóstico , Microscopía Fluorescente , Miocardio/química , Adulto , Anciano , Anciano de 80 o más Años , Amiloidosis/clasificación , Amiloidosis/metabolismo , Biopsia , Errores Diagnósticos/prevención & control , Femenino , Cardiopatías/clasificación , Cardiopatías/metabolismo , Humanos , Técnicas para Inmunoenzimas , Riñón/química , Enfermedades Renales/diagnóstico , Enfermedades Renales/metabolismo , Masculino , Persona de Mediana Edad , Valor Predictivo de las Pruebas , Reproducibilidad de los Resultados , Proteína Amiloide A Sérica/análisis
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