RESUMEN
A boy with X-linked agammaglobulinemia experienced progressive global motor decline, cerebellar syndrome, and epilepsy. All standard polymerase chain reactions for neurotropic viruses were negative on cerebrospinal fluid and brain biopsy. Next-generation sequencing allowed fast identification of a new astrovirus strain (HAstV-VA1/HMO-C-PA), which led to tailor the patient's treatment, with encouraging clinical monitoring over 1 year.
Asunto(s)
Agammaglobulinemia/complicaciones , Infecciones por Astroviridae/tratamiento farmacológico , Infecciones por Astroviridae/virología , Astroviridae/genética , Encefalitis Viral/tratamiento farmacológico , Encefalitis Viral/virología , Enfermedades Genéticas Ligadas al Cromosoma X/complicaciones , Adolescente , Agammaglobulinemia/tratamiento farmacológico , Antiinflamatorios/uso terapéutico , Antivirales/administración & dosificación , Antivirales/uso terapéutico , Astroviridae/clasificación , Astroviridae/aislamiento & purificación , Infecciones por Astroviridae/diagnóstico , Enfermedades Cerebelosas/tratamiento farmacológico , Enfermedades Cerebelosas/etiología , Encefalitis Viral/diagnóstico , Epilepsia/tratamiento farmacológico , Epilepsia/etiología , Enfermedades Genéticas Ligadas al Cromosoma X/tratamiento farmacológico , Genoma Viral , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Masculino , Análisis de Secuencia de ARNRESUMEN
Aedes aegypti is responsible for the transmission of arboviruses. The Yellow Fever, Dengue and Chikungunya viruses are transmitted to the vertebrate host by injection of infected saliva during the blood meal of its vectors. Saliva contains different components with various biochemical activities; anti-hemostatic, angiogenic, inflammatory, and immunomodulatory. This work compares the sialomes of three Ae. aegypti colonies (Rockefeller, PAEA, and Formosus), where the repertoire of salivary proteins from these colonies was analyzed by a proteomic approach. This study indicated that major proteins were detectable in the three colonies. However, differences in the abundance of some saliva proteins have been observed between the three Ae. aegypti colonies.