RESUMEN
A method to recover lymphoid cells from clotted blood for the microcytotoxicity test used in HLA typing is described. The procedure consists of disruption of the clotted blood followed by Ficoll-Hypaque gradient purification of the mononuclear cells. The results of HLA typing of lymphocytes from unclotted and clotted blood were identical.
Asunto(s)
Antígenos HLA , Prueba de Histocompatibilidad , Linfocitos/inmunología , Coagulación Sanguínea , Pruebas Inmunológicas de Citotoxicidad , Humanos , Recuento de LeucocitosRESUMEN
T and B lymphocyte populations were monitored in the peripheral blood of two long-term plateletpheresis donors. The studies showed minimal fluctuation of both populations compared to the normal controls. The evidence so far accumulated seems to indicate that long-term manual plateletpheresis has no appreciable effect on the level of the T and B cell population of the peripheral blood. Further studies are needed to evaluate larger donor pools.