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1.
J Virol ; 84(18): 9463-71, 2010 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-20631139

RESUMEN

Elite controllers maintain undetectable levels of HIV-1 replication in the absence of antiretroviral therapy, but the correlates of immune protection in this patient population are ill defined. Here, we demonstrate that in comparison to patients with progressive HIV-1 infection or healthy persons not infected with HIV-1, elite controllers have circulating myeloid dendritic cells with significantly increased antigen-presenting properties, while their ability to secrete proinflammatory cytokines is substantially diminished. This unique functional profile is associated with a distinct surface expression pattern of immunomodulatory leukocyte-immunoglobulin-like receptors (LILR) and a strong and selective upregulation of LILRB1 and LILRB3. Blockade of these two receptors by monoclonal antibodies or short interfering RNA (siRNA) abrogated the specific antigen-presenting properties of dendritic cells, implying an important regulatory role of these molecules. These data reveal previously unrecognized innate components of immune protection against HIV-1 in elite controllers and offer novel perspectives for the manipulation of host immunity for the prevention and treatment of HIV-1 infection.


Asunto(s)
Presentación de Antígeno , Antígenos CD/metabolismo , Células Dendríticas/inmunología , Infecciones por VIH/inmunología , Sobrevivientes de VIH a Largo Plazo , VIH-1/inmunología , Receptores Inmunológicos/metabolismo , Adulto , Anticuerpos Monoclonales/inmunología , Antígenos CD/inmunología , Citocinas/metabolismo , Femenino , Silenciador del Gen , Humanos , Receptor Leucocitario Tipo Inmunoglobulina B1 , Masculino , Persona de Mediana Edad , Receptores Inmunológicos/antagonistas & inhibidores , Receptores Inmunológicos/inmunología , Adulto Joven
2.
Blood ; 112(9): 3679-87, 2008 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-18728248

RESUMEN

Exhaustion of virus-specific T cells may play an important role in the pathophysiology of chronic viral infections. Here, we analyzed telomere length and telomerase activity in HIV-1-specific CD8+ T cells from progressors or controllers to determine underlying molecular pathways of T-cell exhaustion and senescence. Telomere lengths of HIV-1-specific CD8+ T cells from progressors were significantly shorter compared with autologous cytomegalovirus (CMV)/Epstein-Barr virus (EBV)-specific CD8+ T cells or bulk CD8+ T cells, while telomere lengths from controllers significantly exceeded those of autologous bulk CD8+ T cells and reached a similar level as HIV-1-specific CD8+ T cells collected during primary HIV-1 infection. Telomere length stabilization in controllers corresponded to high levels of constitutive telomerase activity, which was associated with preservation of cytotoxic and proliferative properties. Conversely, limited constitutive telomerase activity was observed in HIV-1-specific CD8+ T cells from progressors, although an increase in both telomere length and telomerase activity was achieved in antigenic-peptide-stimulated cells from progressors after blocking the PD-1/PD ligand 1 (PD-L1) pathway. Collectively, these data suggest a causal role of telomere shortening for the functional deficiencies of HIV-1-specific CD8+ T cells in chronic progressive infection, while high constitutive telomerase activities appears to contribute to maintenance of polyfunctional HIV-1-specific CD8+ T cells from HIV-1 controllers.


Asunto(s)
Linfocitos T CD8-positivos/enzimología , Infecciones por VIH/enzimología , Sobrevivientes de VIH a Largo Plazo , VIH-1 , Telomerasa/metabolismo , Adulto , Antígenos CD/metabolismo , Apoptosis , Proteínas Reguladoras de la Apoptosis/antagonistas & inhibidores , Proteínas Reguladoras de la Apoptosis/metabolismo , Linfocitos T CD8-positivos/inmunología , Linfocitos T CD8-positivos/patología , Proliferación Celular , Citotoxicidad Inmunológica , Infecciones por VIH/inmunología , Infecciones por VIH/patología , VIH-1/inmunología , Humanos , Técnicas In Vitro , Ligandos , Persona de Mediana Edad , Receptor de Muerte Celular Programada 1 , Telómero/patología
3.
J Exp Med ; 206(13): 2959-66, 2009 Dec 21.
Artículo en Inglés | MEDLINE | ID: mdl-20008523

RESUMEN

A subset of HLA-B*35 alleles, B*35-Px, are strongly associated with accelerated HIV-1 disease progression for reasons that are not understood. Interestingly, the alternative set of B*35 subtypes, B*35-PY, have no detectable impact on HIV-1 disease outcomes, even though they can present identical HIV-1 epitopes as B*35-Px molecules. Thus, the differential impact of these alleles on HIV-1 disease progression may be unrelated to interactions with HIV-1-specific CD8(+) T cells. Here, we show that the B*35-Px molecule B*3503 binds with greater affinity to immunoglobulin-like transcript 4 (ILT4), an inhibitory MHC class I receptor expressed on dendritic cells, than does the B*35-PY molecule B*3501, even though these two B*35 molecules differ by only one amino acid and present identical HIV-1 epitopes. The preferential recognition of B*3503 by ILT4 was associated with significantly stronger dendritic cell dysfunction in in vitro functional assays. Moreover, HIV-1-infected carriers of B*3503 had poor dendritic cell functional properties in ex vivo assessments when compared with carriers of the B*3501 allele. Differential interactions between HLA class I allele subtypes and immunoregulatory MHC class I receptors on dendritic cells thus provide a novel perspective for the understanding of MHC class I associations with HIV-1 disease progression and for the manipulation of host immunity against HIV-1.


Asunto(s)
Síndrome de Inmunodeficiencia Adquirida/inmunología , VIH-1 , Antígenos HLA-B/inmunología , Síndrome de Inmunodeficiencia Adquirida/etiología , Células Dendríticas/inmunología , Epítopos de Linfocito T , Genes MHC Clase I , Antígenos HLA-B/genética , Humanos , Glicoproteínas de Membrana/fisiología , Receptores Inmunológicos/fisiología , Linfocitos T Citotóxicos/inmunología
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