circ_0004872 inhibits proliferation, invasion, and glycolysis of oral squamous cell carcinoma by sponged miR-424-5p.

OBJECTIVE: Oral squamous cell carcinoma (OSCC) is one of the most common oral malignant tumors. circ_0004872 can inhibit the progression of gastric cancer, but its effect on the growth and metastasis of OSCC is still unclear. METHODS: qRT-PCR was used to detect the expression levels of circ_0004872 and miR-424-5p in cancer tissues of OSCC patients and adjacent normal tissues, OSCC cell lines, and human normal oral keratinocytes (HOK). CCK-8, cell colony formation, flow cytometry, and transwell assay were used to detect cell proliferation rate, viability, apoptosis rate, and invasion ability. Use glucose/lactic acid kit to assay cell glycolysis ability. The dual-luciferase reporter gene experiment and RIP experiment verified the relationship between circ_0004872 and miR-424-5p. The protein levels were examined by Western blot. RESULTS: The expression of circ_0004872 was significantly downregulated in OSCC tissues and cells, and the overexpression of circ_0004872 inhibited the proliferation, vitality, invasion, and glycolysis of OSCC cells, and promoted apoptosis. The expression of miR-424-5p was greatly upregulated in OSCC tissues and OSCC cells. circ_0004872 can adsorb miR-424-5p in OSCC cells, and circ_0004872 can reverse the promoting effect of miR-424-5p overexpression on the process of OSCC cells. CONCLUSION: circ_0004872 suppresses the proliferation, invasion, and glycolysis of OSCC cells by sponged miR-424-5p, and promotes apoptosis, which can be used as a potential target for early diagnosis and targeted therapy of OSCC.

MiR-92b as a marker for TPF induced chemotherapy response prediction and prognosis evaluation in with advanced oral squamous cell carcinoma patients.

This study aimed to explore the clinical value of miR-92b in advanced oral squamous cell carcinoma (OSCC) and to observe the relationship between miR-92b and TPF induced chemotherapy and prognosis. Totally 114 patients with advanced OSCC admitted to our hospital were selected as the study subjects, all of whom received docetaxel, cisplatin and 5-fluorouracil (TPF) induction chemotherapy. In addition, another 80 healthy subjects who underwent physical examination in our hospital from the same period were enrolled. The serum expression of miR-92b was detected by qRT-PCR. Serum miR-92b was up-regulated in patients with advanced OSCC, and its expression was associated with higher TNM staging and lymph node metastasis. The receiver operating characteristic (ROC) curve revealed that the area under the curve (AUC) of serum miR-92b for the diagnosis of advanced OSCC was 0.931. After treatment, the miR-92b expression was significantly reduced, and the ROC curve showed an AUC value of 0.889 for predicting treatment sensitivity of serum miR-92b. What's more, Logistic indicated that TNM staging, lymph node metastasis and serum miR-92b expression were independent risk factors affecting the treatment efficacy. Survival analysis demonstrated that OSCC patients with high miR-92b expression had poorer OS and DFS compared to patients with low miR-92b expression. Multivariate Cox regression exhibited that TNM staging, lymph node metastasis, and serum miR-92b expression were self-regulating risk factors for overall survival (OS) and disease-free survival (DFS) in patients with advanced OSCC. MiR-92b is up-regulated in patients with advanced OSCC, which can be used as a marker for induction chemotherapy and prognosis evaluation of advanced OSCC.

Expression profile analysis identifies IER3 to predict overall survival and promote lymph node metastasis in tongue cancer.

BACKGROUND: Lymph node metastasis is one of the most important factors affecting the prognosis of tongue cancer, and the molecular mechanism regulating lymph node metastasis of tongue cancer is poorly known. METHODS: The gene expression dataset GSE2280 and The Cancer Genome Atlas (TCGA) tongue cancer dataset were downloaded. R software was used to identify the differentially expressed hallmark gene sets and individual genes between metastatic lymph node tissues and primary tongue cancer tissues, and the Kaplan-Meier method was used to evaluate the association with overall survival. The screening and validation of functional genes was performed using western blot, q-PCR, CCK-8, migration and invasion assays, and lymphangiogenesis was examined by using a tube formation assay. RESULTS: Thirteen common hallmark gene sets were found based on Gene Set Variation Analysis (GSVA) and then subjected to differential gene expression analysis, by which 76 deregulated genes were found. Gene coexpression network analysis and survival analysis further confirmed that IER3 was the key gene associated with the prognosis and lymph node metastasis of tongue cancer patients. Knockdown of IER3 with siRNA inhibited the proliferation, colony formation, migration and invasion of Tca-8113 cells in vitro and it also inhibited the secretion and expression of VEGF-C in these cells. The culture supernatant of Tca-8113 cells could promote lymphangiogenesis and migration of lymphatic endothelial cells, and knockdown of IER3 in Tca-8113 cells suppressed these processes. CONCLUSION: Our study demonstrated that IER3 plays important roles in lymphangiogenesis regulation and prognosis in tongue cancer and might be a potential therapeutic target.

The effect of denture restoration and dental implant restoration in the treatment of dentition defect: a systematic review and meta-analysis.

BACKGROUND: Dentition defect is a common symptom in clinical dental patients. This study compared the clinical effects of denture restoration and dental implant restoration in the treatment of dentition defects through meta-analysis. METHODS: Data retrieval was conducted through the PubMed, Web of Science, Embase, CNKI, and Wanfang databases. A total of 479 related literatures published in English or Chinese from 2013 to 2020 were included. Literature screening, data extraction and comprehensive evaluation, and analysis by meta-analysis was performed by 3 authors. RESULTS: A total of 17 studies and 1,459 patients were included. Among the 17 studies, the effective rate of treatment between the two groups was compared and the experimental group rate was significantly higher than that of the control group [odds ratio (OR) =6.149, 95% confidence interval (CI): 4.103-9.215, P<0.001]; the mastication function score was compared, and was higher in the experimental group than in the control group [standardized mean difference (SMD) =1.632, 95% CI: 1.039-2.224, P<0.001]; the retention function score was compared, and was higher in the experimental group than in the control group (SMD =1.775, 95% CI: 1.095-2.455), P<0.001); the aesthetics score was also compared, and was higher in the experimental group than in the control group (SMD =1.300, 95% CI: 0.499-2.100, P=0.001). Among 17 studies, 15 compared the comfort score, which was higher in the experimental group than in the control group (SMD =1.357, 95% CI: 0.455-2.258, P=0.003). CONCLUSIONS: Compared with denture restoration, dental implant restoration is more effective in the treatment of dentition defect with a higher comprehensive score of functional restoration.