Factors of occurrence of ocular toxoplasmosis. A review.

Acquired and congenital toxoplasmosis are frequently complicated by ocular toxoplasmosis. The diagnosis relies on clinical aspects, response to specific treatment and results of biological assays. The incidence and the prevalence of this complication are difficult to establish precisely and depend on the prevalence of the parasite infection in the general population, and are affected by factors such as type of exposure to the parasite, genetic backgrounds of the parasite and the host, and type of immune response elicited by the parasite.

Contributions of immunoblotting, real-time PCR, and the Goldmann-Witmer coefficient to diagnosis of atypical toxoplasmic retinochoroiditis.

Ocular toxoplasmosis is a major cause of posterior uveitis worldwide. The diagnosis is based mainly on ophthalmological examination. Biological diagnosis is necessary in atypical cases, and this requires aqueous humor sampling by anterior chamber paracentesis. We evaluated real-time PCR targeting the Toxoplasma gondii 529-bp repeat element, the Goldmann-Witmer coefficient (GWC), and immunoblotting for the diagnosis of toxoplasmic retinochoroiditis in 54 patients with atypical uveitis. The results of these biological tests, applied to paired aqueous humor-serum samples, were compared to the clinical findings. Combining either PCR or the GWC with immunoblotting increased the sensitivity to 73% or 70%, respectively. Together, PCR and the GWC had 80% sensitivity. If feasible, sensitivity can be increased by combining the three methods (85% sensitivity). The interval between symptom onset and anterior chamber paracentesis strongly influenced the detection of specific intraocular antibody synthesis. The sensitivity of the GWC increased from 45% to 56% when sampling was performed 10 days after symptom onset, and that of immunoblotting increased from 53% to 72% when puncture was performed 30 days after symptom onset. PCR analysis of aqueous humor samples detected toxoplasmic DNA in 55% of patients. In contrast to the results of immunoblotting and the GWC, the results of PCR were not influenced by the interval between symptom onset and paracentesis. PCR was more informative than the GWC and immunoblotting for immunocompromised patients. Acute necrotizing retinal lesions were significantly larger in PCR-positive patients, with a mean of 3.5 optic disc diameters, than in PCR-negative patients, with a mean of 1.5 optic disc diameters.

[Uveitis associated with thyroïditis in HTLV-1 carriers]. / Uvéite et thyroïdite associée à HTLV-1.

INTRODUCTION: The oncovirus HTLV-1 is aetiologically associated with uveitis and autoimmune thyroiditis in endemic areas. The association of uveitis with autoimmune thyroiditis in HTLV-1 carriers is less common moreover in non-endemic area. EXEGESE: We report two original cases of simultaneous uveitis and autoimmune thyroiditis in HTLV-1 carriers, without other disease due to HTLV-1. The visual outcome was favorable in both cases. CONCLUSION: A significant correlation exists between hyperthyroidism, uveitis and HTLV-1, but still needs to be confirmed. The autoimmune or immune mediated mecanism of HTLV-1 may be involved in the uveitis and the thyroidits.

[Distribution and enantiomorphism of higher-order ocular optical aberrations]. / Etude de la distribution et de l'énantiomorphisme des aberrations optiques oculaires de haut degré.

PURPOSE: To characterize and investigate the higher-order optical aberrations (HOAs) and their distribution and symmetry between both eyes (enantiomorphism). PATIENTS AND METHODS: Wave-front measurements were taken using Hartmann-Shack aberrometry and Zernike polynomial terms reconstruction of the total ocular wave front. Optical aberrations were quantitatively analyzed using the RMS (root mean square) Zernike coefficient term value. Symmetry between both eyes was assessed for each aberration according to two criteria: the RMS coefficient value and aberration magnitude. RESULTS: Sixty patients were included in the study. The HOAs presenting the highest magnitude were fourth-order spherical aberration (Z40; M=0.3038 microm), primary coma (Z31; magnitude M=0.2285 microm) and trefoil (Z33; M=0.1870 microm). A significant mirror symmetry between both eyes was present for high-order aberration orientations. Considering the magnitude of each aberration, symmetry was present in seven of eight aberrations (all but primary coma). HOAs were higher for ametropes than emmetropes. The hyperoptic eyes had more spherical aberrations than myopic and emmetropic eyes. CONCLUSION: Symmetry between the right and left eye is a less common characteristic of HOAs. The difference in the mean age of the studied population may account for the differences in the total high-order and spherical aberration levels found in our study.