RESUMEN
Chronic myeloid leukemia (CML) is characterized by BCR-ABL translocation and an increased number and migration of immature myeloid cells into the peripheral blood. The detection limit of the BCR-ABL transcript, particularly after treatment, is controversial. In the present study, we used quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) to monitor BCR-ABL expression in Moroccan CML patients undergoing imatinib treatment, and compared the results with those of conventional PCR and fluorescence in situ hybridization (FISH). The aim of this study was to establish a new molecular tool for in vitro diagnosis of CML. In a retrospective comparative analysis, 20 CML Moroccan patients who had received imatinib treatment (N = 20) were analyzed by real-time PCR, conventional RT-PCR, and FISH. Half of the samples analyzed (N = 10) were positive for BCR-ABL gene expression, while the other half (N = 10) were negative according to conventional PCR. Interestingly, 5 of the 10 samples shown to be negative by conventional PCR showed positive expression of the BCR-ABL gene according to RT-qPCR. The RT-qPCR results were confirmed by FISH, which revealed a high concordance (100%) rate. We found that real-time RT-qPCR is more reliable and should be used in Moroccan biomedical analysis laboratories to monitor CML progression, particularly for minimal residual disease, following imatinib treatment.
Asunto(s)
Proteínas de Fusión bcr-abl/genética , Leucemia Mielógena Crónica BCR-ABL Positiva/sangre , Leucemia Mielógena Crónica BCR-ABL Positiva/genética , Neoplasia Residual/sangre , Neoplasia Residual/genética , Adulto , Anciano , Femenino , Proteínas de Fusión bcr-abl/sangre , Humanos , Mesilato de Imatinib/efectos adversos , Hibridación Fluorescente in Situ , Leucemia Mielógena Crónica BCR-ABL Positiva/tratamiento farmacológico , Leucemia Mielógena Crónica BCR-ABL Positiva/patología , Masculino , Persona de Mediana Edad , Marruecos , Neoplasia Residual/inducido químicamente , Neoplasia Residual/patología , Patología Molecular , Reacción en Cadena en Tiempo Real de la Polimerasa , Estudios RetrospectivosRESUMEN
Although monitoring of BCR-ABL1 translocation has become an established practice in the management of chronic myeloid leukemia (CML), the detection limit of the BCR-ABL1 transcripts needs more standardization. The aim of the present study was to evaluate the clinical performances of a novel assay for the quantification of BCR-ABL1 fusion transcripts (e13a2 and e14a2) and ABL1 in a single reaction. This assay is based on the real-time reverse transcription polymerase chain reaction (RT-qPCR) in multiplex format. In a retrospective comparative clinical study performed in a reference laboratory, RNA was extracted from 48 CML patient blood samples with various BCR-ABL1/ABL1 ratios and RT-qPCR was performed using either MAScIR assay or the RT-qPCR simplex reference assay used in routine clinical testing. The comparative clinical results showed high qualitative and quantitative concordance (correlation coefficient >0.95) between MAScIR and the reference assays. The present study illustrates the utility of MAScIR assay as a sensitive, rapid, and cost-effective quantitative device to monitor the BCR-ABL1 ratios by RT-qPCR on whole blood of diagnosed Philadelphia chromosome-positive (Ph+) leukemia patients. This test could be used as an aid in the assessment of molecular response to available treatments.
Asunto(s)
Proteínas de Fusión bcr-abl/sangre , Leucemia Mielógena Crónica BCR-ABL Positiva/sangre , Reacción en Cadena de la Polimerasa Multiplex/métodos , ARN Mensajero/sangre , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Translocación Genética , Femenino , Proteínas de Fusión bcr-abl/genética , Humanos , Leucemia Mielógena Crónica BCR-ABL Positiva/genética , Masculino , Cromosoma Filadelfia , ARN Mensajero/genética , Estudios RetrospectivosRESUMEN
The effect of Simulium griseicolle on the blood of growing and laying birds was studied for periods of 10 and eight weeks respectively. The red cell counts and the haemoglobin concentrations were reduced but the mean corpuscular volume was increased. The packed cell volume and the mean corpuscular haemoglobin concentration were only slightly changed. The macrocytic normochromic anaemia thus observed in the infested birds might have been due to nutritional factors rather than to blood loss caused by the feeding insects. Morphological changes were observed in the erythrocytes of the infested birds and these suggest presence of in vivo haemolysis, probably caused by some haemolysing agents in the saliva of the insects.
Asunto(s)
Pollos , Dípteros , Infestaciones Ectoparasitarias/veterinaria , Enfermedades de las Aves de Corral/sangre , Animales , Infestaciones Ectoparasitarias/sangre , Recuento de Eritrocitos , Femenino , Hemoglobinas/análisisRESUMEN
1. Changes in respiratory rate (f), rectal temperature (Tr) and blood acid-base values were measured in laying hens exposed to ambient temperatures (Ta) of 32, 35, 38 or 41 degrees C. 2. At Ta 32 degrees C there was no panting. At Ta 35 degrees C panting occurred without any increase in Tr but there was a slight alkalosis (pH 7.55). 3. At Ta 38 degrees C Tr increased and panting was accompanied by moderate alkalosis (pH 7.58). 4. At Ta 41 degrees C Tr increased considerably and severe alkalosis developed (pH 7.65). 5. From the relation between Tr, f and pH it is concluded that some degree of alkalosis is a normal response to panting in the laying hen.